(Circular) (Six-base) MAPSORT of: pFN21AE3523.seq Check: 1944 from: 1 to: 5752 pFN21AE3523 5752 bp With 182 enzymes: SgfI * November 18, 2016 17:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3095 1937 Size: 1158 4594 Acc65I G'GTAC_C Cuts at: 2643 3073 2643 Size: 430 5322 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2330 2607 2946 2952 3815 5199 5467 1632 Size: 698 277 339 6 863 1384 268 1917 Fragments arranged by size: 1917 1384 863 698 339 277 268 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 2506 4651 5628 1203 Size: 1303 2145 977 1327 Fragments arranged by size: 2145 1327 1303 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AlwNI CAG_nnn'CTG Cuts at: 3495 5067 3495 Size: 1572 4180 ApaLI G'TGCA_C Cuts at: 3809 4965 3809 Size: 1156 4596 ApoI r'AATT_y Cuts at: 1493 3061 3287 3439 5548 1493 Size: 1568 226 152 2109 1697 Fragments arranged by size: 2109 1697 1568 226 152 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 2589 3077 1870 Size: 73 646 488 4545 Fragments arranged by size: 4545 646 488 73 BamHI G'GATC_C Cuts at: 3082 3449 3082 Size: 367 5385 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2643 3073 4528 618 Size: 325 217 627 856 430 1455 1842 Fragments arranged by size: 1842 1455 856 627 430 325 217 BanII G_rGCy'C Cuts at: 1391 2565 3071 1391 Size: 1174 506 4072 Fragments arranged by size: 4072 1174 506 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2940 928 Size: 971 1041 3740 Fragments arranged by size: 3740 1041 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3943 3977 1903 Size: 34 2006 34 3678 Fragments arranged by size: 3678 2006 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2812 3644 4854 2812 Size: 832 1210 3710 Fragments arranged by size: 3710 1210 832 BclI T'GATC_A Cuts at: 1548 1548 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4363 136 Size: 107 122 71 631 605 2691 1525 Fragments arranged by size: 2691 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 2047 3813 4969 1790 Size: 257 1766 1156 2573 Fragments arranged by size: 2573 1766 1156 257 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2404 3483 4441 Size: 416 53 83 399 24 987 1079 958 Cuts at: 4441 442 Size: 1753 Fragments arranged by size: 1753 1079 987 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2801 4412 1239 Size: 1562 1611 2579 Fragments arranged by size: 2579 1611 1562 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3877 4742 5040 3877 Size: 865 298 4589 Fragments arranged by size: 4589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2964 4415 882 Size: 617 1465 1451 2219 Fragments arranged by size: 2219 1465 1451 617 BsaAI yAC'GTr Cuts at: 493 1204 3543 493 Size: 711 2339 2702 Fragments arranged by size: 2702 2339 711 BsaBI GATnn'nnATC Cuts at: 5746 5746 Size: 5752 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3559 3941 4576 275 Size: 53 83 186 936 2026 382 635 1451 Fragments arranged by size: 2026 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4185 4583 4857 5004 5415 4185 Size: 398 274 147 411 4522 Fragments arranged by size: 4522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2065 2095 2927 2957 2963 Size: 30 165 30 662 30 832 30 6 Cuts at: 2963 2993 1178 Size: 30 3937 Fragments arranged by size: 3937 832 662 165 30 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5752 BseYI C'CCAG_C Cuts at: 2176 2606 2665 2800 2911 4955 5451 2176 Size: 430 59 135 111 2044 496 2477 Fragments arranged by size: 2477 2044 496 430 135 111 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2727 1163 Size: 573 991 4188 Fragments arranged by size: 4188 991 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3202 3963 4991 1540 Size: 275 186 1201 761 1028 2301 Fragments arranged by size: 2301 1201 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 2565 3813 3898 4969 2340 Size: 225 1248 85 1071 3123 Fragments arranged by size: 3123 1248 1071 225 85 BsmI GAATG_Cn' Cuts at: 2266 3263 3356 2266 Size: 997 93 4662 Fragments arranged by size: 4662 997 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2983 2983 Size: 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2340 2565 3071 3813 3898 4969 Size: 399 257 293 225 506 742 85 1071 Cuts at: 4969 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 506 399 293 257 225 85 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 3001 3641 5371 3001 Size: 640 1730 3382 Fragments arranged by size: 3382 1730 640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3107 844 Size: 2263 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3639 1394 Size: 429 1816 3507 Fragments arranged by size: 3507 1816 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4247 4421 65 Size: 1183 2999 174 1396 Fragments arranged by size: 2999 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2646 4396 4583 1700 Size: 110 836 1750 187 2869 Fragments arranged by size: 2869 1750 836 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 3812 4824 3812 Size: 1012 4740 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5752 BstBI TT'CG_AA Cuts at: 4556 4556 Size: 5752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstYI r'GATC_y Cuts at: 1972 2101 3082 3449 3834 3851 5303 5389 5401 Size: 129 981 367 385 17 1452 86 12 Cuts at: 5401 5747 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 981 385 367 346 129 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2069 513 Size: 552 1004 4196 Fragments arranged by size: 4196 1004 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5013 Fragments arranged by size: 5013 683 56 BtsI GCAGTG_nn' Cuts at: 2870 3268 4062 4082 2870 Size: 398 794 20 4540 Fragments arranged by size: 4540 794 398 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5717 DraI TTT'AAA Cuts at: 2244 3056 2244 Size: 812 4940 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2825 3199 4088 Size: 54 1425 50 275 18 995 374 889 Cuts at: 4088 5717 8 Size: 1629 43 Fragments arranged by size: 1629 1425 995 889 374 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 1537 Size: 275 1387 4090 Fragments arranged by size: 4090 1387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2091 2123 2626 3682 1353 Size: 738 32 503 1056 3423 Fragments arranged by size: 3423 1056 738 503 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2971 4339 4713 4859 387 Size: 1064 138 1382 1368 374 146 1280 Fragments arranged by size: 1382 1368 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2563 2563 Size: 5752 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2607 2801 2946 2952 3815 4412 Size: 393 698 277 194 145 6 863 597 Cuts at: 4412 5199 5467 1239 Size: 787 268 1524 Fragments arranged by size: 1524 863 787 698 597 393 277 268 194 145 6 EcoO109I rG'GnC_Cy Cuts at: 2482 2521 2482 Size: 39 5713 EcoRI G'AATT_C Cuts at: 3061 3439 3061 Size: 378 5374 FspI TGC'GCA Cuts at: 4258 4258 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 3018 4899 1678 Size: 1340 1881 2531 Fragments arranged by size: 2531 1881 1340 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2927 2959 2961 2963 2993 Size: 32 135 32 1355 32 2 2 30 Cuts at: 2993 2995 4393 4425 4467 4499 1373 Size: 2 1398 32 42 32 2626 Fragments arranged by size: 2626 1398 1355 135 42 32 32 32 32 32 30 2 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2741 3096 3342 669 Size: 988 281 803 355 246 3079 Fragments arranged by size: 3079 988 803 355 281 246 HindIII A'AGCT_T Cuts at: 748 2298 748 Size: 1550 4202 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KpnI G_GTAC'C Cuts at: 2647 3077 2647 Size: 430 5322 MfeI C'AATT_G Cuts at: 3351 3351 Size: 5752 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2787 4866 5050 1305 Size: 1482 2079 184 2007 Fragments arranged by size: 2079 2007 1482 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3930 Fragments arranged by size: 3930 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 3710 4069 4228 518 Size: 648 1089 1455 359 159 2042 Fragments arranged by size: 2042 1455 1089 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2779 3031 3168 3845 4993 5238 1606 Size: 1173 252 137 677 1148 245 2120 Fragments arranged by size: 2120 1173 1148 677 252 245 137 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5200 NdeI CA'TA_TG Cuts at: 387 2699 387 Size: 2312 3440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 2327 2395 3110 4655 1132 Size: 97 1098 68 715 1545 2229 Fragments arranged by size: 2229 1545 1098 715 97 68 PasI CC'CwG_GG Cuts at: 1348 2022 1348 Size: 674 5078 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5752 PfoI T'CCnGG_A Cuts at: 2428 2428 Size: 5752 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2521 2521 Size: 5752 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5752 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PstI C_TGCA'G Cuts at: 830 3104 830 Size: 2274 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 PvuII CAG'CTG Cuts at: 2779 3031 2779 Size: 252 5500 SacI G_AGCT'C Cuts at: 2565 2565 Size: 5752 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SapI GCTCTTCn'nnn_ Cuts at: 2123 2123 Size: 5752 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SfcI C'TryA_G Cuts at: 826 1045 2811 3100 4235 4916 5107 826 Size: 219 1766 289 1135 681 191 1471 Fragments arranged by size: 1766 1471 1135 681 289 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 2591 3079 1872 Size: 719 488 4545 Fragments arranged by size: 4545 719 488 SmlI C'TyrA_G Cuts at: 820 1017 1943 3856 4757 5019 820 Size: 197 926 1913 901 262 1553 Fragments arranged by size: 1913 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 2233 152 Size: 2081 3671 SphI G_CATG'C Cuts at: 1132 3110 1132 Size: 1978 3774 SrfI GCCC'GGGC Cuts at: 2591 2591 Size: 5752 SspI AAT'ATT Cuts at: 5 52 2332 2681 3676 5 Size: 47 2280 349 995 2081 Fragments arranged by size: 2280 2081 995 349 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5752 StyI C'CwwG_G Cuts at: 513 1065 3191 513 Size: 552 2126 3074 Fragments arranged by size: 3074 2126 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2168 3779 3796 3949 4134 4579 325 Size: 1255 588 1611 17 153 185 445 1498 Fragments arranged by size: 1611 1498 1255 588 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3998 5710 96 Size: 274 80 33 51 1443 2021 1712 138 Fragments arranged by size: 2021 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2065 2097 2220 2252 5712 5744 2065 Size: 32 123 32 3460 32 2073 Fragments arranged by size: 3460 2073 123 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2975 1316 Size: 1659 4093 XbaI T'CTAG_A Cuts at: 3088 3088 Size: 5752 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 2589 3077 1870 Size: 719 488 4545 Fragments arranged by size: 4545 719 488 XmnI GAAnn'nnTTC Cuts at: 3881 3881 Size: 5752 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SwaI XcmI