(Circular) (Six-base) MAPSORT of: pFN21AB5005.seq Check: 3583 from: 1 to: 5959 pFN21AB5005 5959 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2143 2894 4022 5406 5674 1632 Size: 511 751 1128 1384 268 1917 Fragments arranged by size: 1917 1384 1128 751 511 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2528 1184 Size: 1344 4615 AlwNI CAG_nnn'CTG Cuts at: 2093 3702 5274 2093 Size: 1609 1572 2778 Fragments arranged by size: 2778 1609 1572 ApaLI G'TGCA_C Cuts at: 2324 4016 5172 2324 Size: 1692 1156 3111 Fragments arranged by size: 3111 1692 1156 ApoI r'AATT_y Cuts at: 1493 2642 3268 3494 3646 5755 1493 Size: 1149 626 226 152 2109 1697 Fragments arranged by size: 2109 1697 1149 626 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2561 2837 3284 1870 Size: 73 618 276 447 4545 Fragments arranged by size: 4545 618 447 276 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2594 3216 3280 4735 618 Size: 325 217 627 807 622 64 1455 1842 Fragments arranged by size: 1842 1455 807 627 622 325 217 64 BanII G_rGCy'C Cuts at: 1391 2117 2244 3278 1391 Size: 726 127 1034 4072 Fragments arranged by size: 4072 1034 726 127 BbeI G_GCGC'C Cuts at: 2598 3220 2598 Size: 622 5337 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4150 4184 1903 Size: 34 50 34 2129 34 3678 Fragments arranged by size: 3678 2129 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2153 4570 136 Size: 107 122 71 631 605 481 2417 1525 Fragments arranged by size: 2417 1525 631 605 481 122 107 71 BglII A'GATC_T Cuts at: 2877 5954 2877 Size: 3077 2882 BlpI GC'TnA_GC Cuts at: 2294 3376 2294 Size: 1082 4877 Bme1580I G_kGCm'C Cuts at: 1790 2328 4020 5176 1790 Size: 538 1692 1156 2573 Fragments arranged by size: 2573 1692 1156 538 BmgBI CAC'GTC Cuts at: 1318 2578 1318 Size: 1260 4699 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2641 3690 4648 Size: 416 53 83 399 24 1224 1049 958 Cuts at: 4648 442 Size: 1753 Fragments arranged by size: 1753 1224 1049 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2782 4619 1239 Size: 824 719 1837 2579 Fragments arranged by size: 2579 1837 824 719 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2827 3750 493 Size: 711 1623 923 2702 Fragments arranged by size: 2702 1623 923 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2273 2595 3217 3766 Size: 53 83 186 936 740 322 622 549 Cuts at: 3766 4148 4783 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 740 635 622 549 382 322 186 83 53 BsaWI w'CCGG_w Cuts at: 2718 4392 4790 5064 5211 5622 2718 Size: 1674 398 274 147 411 3055 Fragments arranged by size: 3055 1674 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2780 2810 1178 Size: 30 165 30 1377 30 4327 Fragments arranged by size: 4327 1377 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 3140 1186 Size: 1147 807 4005 Fragments arranged by size: 4005 1147 807 BseYI C'CCAG_C Cuts at: 2236 2251 2392 2414 2969 2974 5162 5658 2236 Size: 15 141 22 555 5 2188 496 2537 Fragments arranged by size: 2537 2188 555 496 141 22 15 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 2478 4020 4105 5176 2328 Size: 150 1542 85 1071 3111 Fragments arranged by size: 3111 1542 1071 150 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 BsmBI CGTCTCn'nnnn_ Cuts at: 3243 3243 Size: 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2244 2328 2478 3278 4020 4105 Size: 399 327 127 84 150 800 742 85 Cuts at: 4105 5176 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 800 742 399 327 150 127 85 84 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2706 3848 5578 2706 Size: 1142 1730 3087 Fragments arranged by size: 3087 1730 1142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2083 2961 3314 844 Size: 1239 878 353 3489 Fragments arranged by size: 3489 1239 878 353 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 2330 4603 4790 1700 Size: 110 337 183 2273 187 2869 Fragments arranged by size: 2869 2273 337 187 183 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 2372 2508 1925 Size: 447 136 5376 Fragments arranged by size: 5376 447 136 BssSI C'ACGA_G Cuts at: 2243 4019 5031 2243 Size: 1776 1012 3171 Fragments arranged by size: 3171 1776 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 2566 2941 1184 Size: 1382 375 4202 Fragments arranged by size: 4202 1382 375 BstXI CCAn_nnnn'nTGG Cuts at: 2578 2932 2578 Size: 354 5605 BstYI r'GATC_y Cuts at: 1972 2877 3289 3656 4041 4058 5510 5596 5608 Size: 905 412 367 385 17 1452 86 12 Cuts at: 5608 5954 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 905 412 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3231 3231 Size: 5959 BtgI C'CryG_G Cuts at: 513 1065 2175 2217 2268 2367 2668 2697 513 Size: 552 1110 42 51 99 301 29 3775 Fragments arranged by size: 3775 1110 552 301 99 51 42 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 2097 2199 2295 3475 4269 4289 2097 Size: 102 96 1180 794 20 3767 Fragments arranged by size: 3767 1180 794 102 96 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3055 3090 529 Size: 35 2491 35 3398 Fragments arranged by size: 3398 2491 35 35 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2272 4966 809 Size: 507 956 2694 1802 Fragments arranged by size: 2694 1802 956 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2124 2178 Size: 54 1425 50 275 18 225 69 54 Cuts at: 2178 3048 3406 4295 5924 8 Size: 870 358 889 1629 43 Fragments arranged by size: 1629 1425 889 870 358 275 225 69 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3889 1353 Size: 2536 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2143 2782 2894 4022 4619 5406 Size: 393 431 80 639 112 1128 597 787 Cuts at: 5406 5674 1239 Size: 268 1524 Fragments arranged by size: 1524 1128 787 639 597 431 393 268 112 80 EcoNI CCTnn'n_nnAGG Cuts at: 2042 2984 2042 Size: 942 5017 EcoO109I rG'GnC_Cy Cuts at: 3037 3037 Size: 5959 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2506 3028 2506 Size: 522 5437 FspI TGC'GCA Cuts at: 2447 3134 4465 2447 Size: 687 1331 3941 Fragments arranged by size: 3941 1331 687 HaeII r_GCGC'y Cuts at: 1678 2031 2148 2301 2598 2845 3220 5106 1678 Size: 353 117 153 297 247 375 1886 2531 Fragments arranged by size: 2531 1886 375 353 297 247 153 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373 Size: 32 135 32 3028 32 42 32 2626 Fragments arranged by size: 3028 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2608 3303 3549 669 Size: 988 281 670 695 246 3079 Fragments arranged by size: 3079 988 695 670 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2594 3216 2594 Size: 622 5337 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3060 5073 5257 1305 Size: 1755 2013 184 2007 Fragments arranged by size: 2013 2007 1755 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2180 3050 10 Size: 54 1425 343 348 870 2919 Fragments arranged by size: 2919 1425 870 348 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 2634 2711 2813 3917 4276 4435 Size: 648 1041 427 77 102 1104 359 159 Cuts at: 4435 518 Size: 2042 Fragments arranged by size: 2042 1104 1041 648 427 359 159 102 77 MspA1I CmG'CkG Cuts at: 1606 2308 2872 3375 4052 5200 5445 1606 Size: 702 564 503 677 1148 245 2120 Fragments arranged by size: 2120 1148 702 677 564 503 245 NaeI GCC'GGC Cuts at: 2149 2332 2149 Size: 183 5776 NarI GG'CG_CC Cuts at: 2595 3217 2595 Size: 622 5337 NcoI C'CATG_G Cuts at: 513 1065 2175 513 Size: 552 1110 4297 Fragments arranged by size: 4297 1110 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NgoMIV G'CCGG_C Cuts at: 2147 2330 2147 Size: 183 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3025 3317 4862 1132 Size: 97 1796 292 1545 2229 Fragments arranged by size: 2229 1796 1545 292 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 2176 2194 2656 2827 1108 Size: 1068 18 462 171 4240 Fragments arranged by size: 4240 1068 462 171 18 PfoI T'CCnGG_A Cuts at: 2040 2040 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PmlI CAC'GTG Cuts at: 2827 2827 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3037 3037 Size: 5959 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 2080 2517 2958 3311 830 Size: 1250 437 441 353 3478 Fragments arranged by size: 3478 1250 441 437 353 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2308 2872 2308 Size: 564 5395 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2076 2513 2954 3307 4442 5123 5314 Size: 219 1031 437 441 353 1135 681 191 Cuts at: 5314 826 Size: 1471 Fragments arranged by size: 1471 1135 1031 681 441 437 353 219 191 SfoI GGC'GCC Cuts at: 2596 3218 2596 Size: 622 5337 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2837 4063 4964 5226 820 Size: 197 926 894 1226 901 262 1553 Fragments arranged by size: 1553 1226 926 901 894 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 2727 1517 Size: 1210 4749 StyI C'CwwG_G Cuts at: 513 1065 2118 2175 2181 2283 2571 2925 3398 Size: 552 1053 57 6 102 288 354 473 Cuts at: 3398 513 Size: 3074 Fragments arranged by size: 3074 1053 552 473 354 288 102 57 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2676 2690 3203 3986 4003 4156 4341 Size: 1255 1096 14 513 783 17 153 185 Cuts at: 4341 4786 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1096 783 513 445 185 153 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4205 5917 96 Size: 274 80 33 51 1443 2228 1712 138 Fragments arranged by size: 2228 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2780 2812 5919 5951 2780 Size: 32 3107 32 2788 Fragments arranged by size: 3107 2788 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2686 1316 Size: 1370 4589 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2932 2932 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 2837 1943 Size: 894 5065 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI