Restriction map for
pFN21AB5004
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AlwI Aor51HI AscI AseI AsnI
Asp700I AvaII AviII BalI BamHI
BbrPI BbvCI BciVI BclI BfrI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BspDI
BspEI BspMI BsrBI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstYI CelII ClaI CpoI DraI
DraIII EagI EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI MvnI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 325 GACGTC GACGT^C
AccI 76 GTATAC GT^[AC][GT]AC
AcsI 958 GAATTT [GA]^AATT[CT]
AflIII 572 ACATGT A^C[GA][CT]GT
AhdI 117 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 958 GAATTT [GA]^AATT[CT]
AspEI 117 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 259 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 405 CCTAGG C^CTAGG
BcgI 692 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 705 GCCTGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 405 CCTAGG C^CTAGG
BseRI 1134 CTCCTC GAGGAG(10/8)
BsgI 527 CTGCAC GTGCAG(16/14)
BsiEI 597 CGACCG CG[GA][CT]^CG
BsmBI 1131 CGTCTC CGTCTC(1/5)
BspHI 1071 TCATGA T^CATGA
BspLU11I 572 ACATGT A^CATGT
BsrDI 305 GCAATG GCAATG(2/0)
Bst1107I 76 GTATAC GTA^TAC
BstXI 554 CCACAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 76 GTATAC GTA^TAC
EaeI 1031 TGGCCG [CT]^GGCC[GA]
Eam1105I 117 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 937 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1075 GATATC GAT^ATC
HgaI 123 GCGTC GACGC(5/10)
MaeII 326 ACGT A^CGT
MseI 812 TTAA T^TAA
NaeI 872 GCCGGC GCC^GGC
NcoI 1140 CCATGG C^CATGG
NgoMIV 872 GCCGGC G^CCGGC
PflFI 259 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 491 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 53 CTGCAG CTGCA^G
RcaI 1071 TCATGA T^CATGA
SfaNI 69 GCATC GCATC(5/9)
SfcI 53 CTGCAG C^T[GA][CT]AG
SgrAI 871 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 380 CCCGGG CCC^GGG
StuI 945 AGGCCT AGG^CCT
TfiI 1038 GATTC G^A[AT]TC
Tru9I 812 TTAA T^TAA
Tsp509I 959 AATT ^AATT
Tth111I 259 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 491 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 723 CCACTCGGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 380 CCCGGG C^CCGGG
XmaI 380 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 216 GGTACC G^GTACC
922 GGTACC
Alw44I 16 GTGCAC G^TGCAC
136 GTGCAC
AlwNI 56 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
368 CAGTTCCTG
AocI 706 CCTGAGG CC^T[ACGT]AGG
937 CCTGAGG
ApaI 31 GGGCCC GGGCC^C
377 GGGCCC
ApaLI 16 GTGCAC G^TGCAC
136 GTGCAC
Asp718I 216 GGTACC G^GTACC
922 GGTACC
AvaI 353 CCCGAG C^[CT]CG[GA]G
380 CCCGGG
BanII 31 GGGCCC G[GA]GC[CT]^C
377 GGGCCC
BbeI 713 GGCGCC GGCGC^C
875 GGCGCC
BbsI 106 GAAGAC GAAGAC(2/6)
1014 GAAGAC
BpuAI 106 GAAGAC GAAGAC(2/6)
1014 GAAGAC
BsmAI 115 GAGAC GTCTC(1/5)
1132 GTCTC
BsmI 1092 GAATGC GAATGC(1/-1)
644 GCATTC
BsoBI 353 CCCGAG C^[CT]CG[GA]G
380 CCCGGG
BsrFI 785 ACCGGC [GA]^CCGG[CT]
872 GCCGGC
BstF5I 195 CATCC GGATG(2/0)
384 GGATG
Bsu36I 706 CCTGAGG CC^T[ACGT]AGG
937 CCTGAGG
Cfr10I 785 ACCGGC [GA]^CCGG[CT]
872 GCCGGC
DpnII 414 GATC ^GATC
973 GATC
DrdI 256 GACGACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
901 GACAAGATTGTC
DsaI 198 CCGTGG C^C[GA][CT]GG
1140 CCATGG
Eco81I 706 CCTGAGG CC^T[ACGT]AGG
937 CCTGAGG
EcoT14I 405 CCTAGG C^C[AT][AT]GG
1140 CCATGG
FokI 195 CATCC GGATG(9/13)
384 GGATG
HaeII 713 GGCGCC [GA]GCGC^[CT]
875 GGCGCC
KasI 713 GGCGCC G^GCGCC
875 GGCGCC
KpnI 216 GGTACC GGTAC^C
922 GGTACC
MboI 414 GATC ^GATC
973 GATC
MboII 106 GAAGA GAAGA(8/7)
1014 GAAGA
MslI 571 CACATGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
790 CACTTCCATG
NarI 713 GGCGCC GG^CGCC
875 GGCGCC
NdeII 414 GATC ^GATC
973 GATC
NspI 224 ACATGC [GA]CATG^[CT]
572 ACATGT
Sau3AI 414 GATC ^GATC
973 GATC
SfoI 713 GGCGCC GGC^GCC
875 GGCGCC
StyI 405 CCTAGG C^C[AT][AT]GG
1140 CCATGG
TaqI 254 TCGA T^CGA
648 TCGA
Tsp45I 569 GTCAC ^GT[GC]AC
899 GTGAC
TthHB8I 254 TCGA T^CGA
648 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
BanI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SspBI
TseI TspRI