(Circular) (Six-base) MAPSORT of: pFN21AB5004.seq Check: 7256 from: 1 to: 5851 pFN21AB5004 5851 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2334 3661 278 Size: 53 83 186 936 798 1327 2468 Fragments arranged by size: 2468 1327 936 798 186 83 53 AccI GT'mk_AC Cuts at: 1937 2082 3194 1937 Size: 145 1112 4594 Fragments arranged by size: 4594 1112 145 Acc65I G'GTAC_C Cuts at: 2221 2927 3172 2221 Size: 706 245 4900 Fragments arranged by size: 4900 706 245 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3038 3914 5298 5566 1632 Size: 1406 876 1384 268 1917 Fragments arranged by size: 1917 1406 1384 876 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 2577 4750 5727 1203 Size: 1374 2173 977 1327 Fragments arranged by size: 2173 1374 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 2127 4580 2127 Size: 2453 3398 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5851 AlwNI CAG_nnn'CTG Cuts at: 2066 2378 3594 5166 2066 Size: 312 1216 1572 2751 Fragments arranged by size: 2751 1572 1216 312 ApaI G_GGCC'C Cuts at: 2040 2386 2040 Size: 346 5505 ApaLI G'TGCA_C Cuts at: 2021 2141 3908 5064 2021 Size: 120 1767 1156 2808 Fragments arranged by size: 2808 1767 1156 120 ApoI r'AATT_y Cuts at: 1493 2963 3160 3386 3538 5647 1493 Size: 1470 197 226 152 2109 1697 Fragments arranged by size: 2109 1697 1470 226 197 152 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 2358 2385 3176 1870 Size: 73 415 27 791 4545 Fragments arranged by size: 4545 791 415 73 27 AvrII C'CTAG_G Cuts at: 2410 2410 Size: 5851 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2221 2718 2880 2927 3172 Size: 325 217 627 434 497 162 47 245 Cuts at: 3172 4627 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 497 434 325 245 217 162 47 BanII G_rGCy'C Cuts at: 1391 2040 2386 3170 1391 Size: 649 346 784 4072 Fragments arranged by size: 4072 784 649 346 BbeI G_GCGC'C Cuts at: 2722 2884 2722 Size: 162 5689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2118 3026 928 Size: 971 219 908 3753 Fragments arranged by size: 3753 971 908 219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2686 2720 4042 4076 1903 Size: 34 749 34 1322 34 3678 Fragments arranged by size: 3678 1322 749 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3743 4953 3743 Size: 1210 4641 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2716 4462 136 Size: 107 122 71 631 605 1044 1746 1525 Fragments arranged by size: 1746 1525 1044 631 605 122 107 71 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 3268 3268 Size: 5851 Bme1580I G_kGCm'C Cuts at: 1790 2025 2040 2145 2386 3912 5068 1790 Size: 235 15 105 241 1526 1156 2573 Fragments arranged by size: 2573 1526 1156 241 235 105 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3582 4540 442 Size: 416 53 83 399 24 2165 958 1753 Fragments arranged by size: 2165 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2560 2685 4511 1239 Size: 809 512 125 1826 2579 Fragments arranged by size: 2579 1826 809 512 125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3976 4841 5139 3976 Size: 865 298 4688 Fragments arranged by size: 4688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4514 882 Size: 617 3015 2219 Fragments arranged by size: 3015 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3642 493 Size: 711 2438 2702 Fragments arranged by size: 2702 2438 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2331 2719 2881 3658 Size: 53 83 186 936 798 388 162 777 Cuts at: 3658 4040 4675 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 798 777 635 388 382 186 162 83 53 BsaWI w'CCGG_w Cuts at: 4284 4682 4956 5103 5514 4284 Size: 398 274 147 411 4621 Fragments arranged by size: 4621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5626 Fragments arranged by size: 5626 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3130 1186 Size: 1944 3907 BseYI C'CCAG_C Cuts at: 2566 5054 5550 2566 Size: 2488 496 2867 Fragments arranged by size: 2867 2488 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2517 1163 Size: 573 781 4497 Fragments arranged by size: 4497 781 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2605 3301 4062 5090 1540 Size: 275 186 604 696 761 1028 2301 Fragments arranged by size: 2301 1028 761 696 604 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 2145 2319 3912 3997 5068 2025 Size: 120 174 1593 85 1071 2808 Fragments arranged by size: 2808 1593 1071 174 120 85 BsmI GAATG_Cn' Cuts at: 2649 3103 3362 3455 2649 Size: 454 259 93 5045 Fragments arranged by size: 5045 454 259 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3142 3142 Size: 5851 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2040 2145 2319 2386 3170 3912 Size: 399 235 15 105 174 67 784 742 Cuts at: 3912 3997 5068 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 784 742 399 235 174 105 85 67 15 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 3076 3740 5470 3076 Size: 664 1730 3457 Fragments arranged by size: 3457 1730 664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3206 844 Size: 2362 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3738 1394 Size: 429 1915 3507 Fragments arranged by size: 3507 1915 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2317 4346 4520 65 Size: 1183 1069 2029 174 1396 Fragments arranged by size: 2029 1396 1183 1069 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2790 2877 4495 4682 1700 Size: 110 980 87 1618 187 2869 Fragments arranged by size: 2869 1618 980 187 110 87 BsrGI T'GTAC_A Cuts at: 96 2424 2516 2757 96 Size: 2328 92 241 3190 Fragments arranged by size: 3190 2328 241 92 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 3911 4923 3911 Size: 1012 4839 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5851 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5851 BstXI CCAn_nnnn'nTGG Cuts at: 2566 2566 Size: 5851 BstYI r'GATC_y Cuts at: 1972 3181 3548 3933 3950 5402 5488 5500 5846 Size: 1209 367 385 17 1452 86 12 346 Cuts at: 5846 1972 Size: 1977 Fragments arranged by size: 1977 1452 1209 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2083 2083 Size: 5851 Bsu36I CC'TnA_GG Cuts at: 2712 2943 2712 Size: 231 5620 BtgI C'CryG_G Cuts at: 513 1065 2203 3145 513 Size: 552 1138 942 3219 Fragments arranged by size: 3219 1138 942 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5112 Fragments arranged by size: 5112 683 56 BtsI GCAGTG_nn' Cuts at: 2067 2123 2163 3367 4161 4181 2067 Size: 56 40 1204 794 20 3737 Fragments arranged by size: 3737 1204 794 56 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2267 2912 4858 809 Size: 507 951 645 1946 1802 Fragments arranged by size: 1946 1802 951 645 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3036 3298 4187 Size: 54 1425 50 275 18 1206 262 889 Cuts at: 4187 5816 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1206 889 275 262 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 1537 Size: 275 1486 4090 Fragments arranged by size: 4090 1486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3781 1353 Size: 2428 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2302 2468 4438 4812 4958 387 Size: 1064 138 713 166 1970 374 146 1280 Fragments arranged by size: 1970 1280 1064 713 374 166 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2560 2685 3038 3914 4511 5298 Size: 393 416 512 125 353 876 597 787 Cuts at: 5298 5566 1239 Size: 268 1524 Fragments arranged by size: 1524 876 787 597 512 416 393 353 268 125 EcoNI CCTnn'n_nnAGG Cuts at: 2946 2946 Size: 5851 EcoO109I rG'GnC_Cy Cuts at: 2037 2304 2382 2037 Size: 267 78 5506 Fragments arranged by size: 5506 267 78 EcoRI G'AATT_C Cuts at: 3160 3538 3160 Size: 378 5473 EcoRV GAT'ATC Cuts at: 3082 3082 Size: 5851 FspI TGC'GCA Cuts at: 4357 4357 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 2722 2884 4998 1678 Size: 1044 162 2114 2531 Fragments arranged by size: 2531 2114 1044 162 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2113 2145 2491 2523 2537 Size: 32 135 32 541 32 346 32 14 Cuts at: 2537 2569 4492 4524 4566 4598 1373 Size: 32 1923 32 42 32 2626 Fragments arranged by size: 2626 1923 541 346 135 42 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 3195 3441 669 Size: 988 281 1257 246 3079 Fragments arranged by size: 3079 1257 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KasI G'GCGC_C Cuts at: 2718 2880 2718 Size: 162 5689 KpnI G_GTAC'C Cuts at: 2225 2931 3176 2225 Size: 706 245 4900 Fragments arranged by size: 4900 706 245 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2150 2649 4965 5149 1305 Size: 845 499 2316 184 2007 Fragments arranged by size: 2316 2007 845 499 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4029 Fragments arranged by size: 4029 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2580 2799 3809 4168 4327 518 Size: 648 1414 219 1010 359 159 2042 Fragments arranged by size: 2042 1414 1010 648 359 219 159 MspA1I CmG'CkG Cuts at: 1606 3267 3944 5092 5337 1606 Size: 1661 677 1148 245 2120 Fragments arranged by size: 2120 1661 1148 677 245 NaeI GCC'GGC Cuts at: 2879 2879 Size: 5851 NarI GG'CG_CC Cuts at: 2719 2881 2719 Size: 162 5689 NcoI C'CATG_G Cuts at: 513 1065 3145 513 Size: 552 2080 3219 Fragments arranged by size: 3219 2080 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NgoMIV G'CCGG_C Cuts at: 2877 2877 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 2233 2581 3209 4754 1132 Size: 97 1004 348 628 1545 2229 Fragments arranged by size: 2229 1545 1004 628 348 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5851 PciI A'CATG_T Cuts at: 2577 4750 2577 Size: 2173 3678 PflMI CCAn_nnn'nTGG Cuts at: 1108 2502 1108 Size: 1394 4457 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 2775 3421 2775 Size: 646 5205 PspOMI G'GGCC_C Cuts at: 2036 2382 2036 Size: 346 5505 PstI C_TGCA'G Cuts at: 830 2062 3203 830 Size: 1232 1141 3478 Fragments arranged by size: 3478 1232 1141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SfcI C'TryA_G Cuts at: 826 1045 2058 3199 4334 5015 5206 826 Size: 219 1013 1141 1135 681 191 1471 Fragments arranged by size: 1471 1141 1135 1013 681 219 191 SfoI GGC'GCC Cuts at: 2720 2882 2720 Size: 162 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 2877 1700 Size: 1177 4674 SmaI CCC'GGG Cuts at: 1872 2387 3178 1872 Size: 515 791 4545 Fragments arranged by size: 4545 791 515 SmlI C'TyrA_G Cuts at: 820 1017 1943 3955 4856 5118 820 Size: 197 926 2012 901 262 1553 Fragments arranged by size: 2012 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 3209 1132 Size: 2077 3774 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 2952 1517 Size: 1435 4416 StyI C'CwwG_G Cuts at: 513 1065 2410 3145 3290 513 Size: 552 1345 735 145 3074 Fragments arranged by size: 3074 1345 735 552 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3878 3895 4048 4233 4678 325 Size: 1255 2298 17 153 185 445 1498 Fragments arranged by size: 2298 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 2516 2757 Size: 274 80 33 51 1443 447 92 241 Cuts at: 2757 4097 5809 96 Size: 1340 1712 138 Fragments arranged by size: 1712 1443 1340 447 274 241 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5811 5843 5811 Size: 32 5819 Tth111I GACn'n_nGTC Cuts at: 1316 2267 1316 Size: 951 4900 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2735 2735 Size: 5851 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 2385 3176 1870 Size: 515 791 4545 Fragments arranged by size: 4545 791 515 XmnI GAAnn'nnTTC Cuts at: 3980 3980 Size: 5851 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2332 3659 276 Size: 53 83 186 936 798 1327 2468 Fragments arranged by size: 2468 1327 936 798 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI