(Circular) (Six-base) MAPSORT of: pFN21AB4987.seq Check: 3182 from: 1 to: 6118 pFN21AB4987 6118 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2973 3928 278 Size: 53 83 186 936 1437 955 2468 Fragments arranged by size: 2468 1437 955 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3461 1937 Size: 1524 4594 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 2523 2854 4181 5565 5833 1632 Size: 633 258 331 1327 1384 268 1917 Fragments arranged by size: 1917 1384 1327 633 331 268 258 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 3195 5017 5994 1203 Size: 1992 1822 977 1327 Fragments arranged by size: 1992 1822 1327 977 AgeI A'CCGG_T Cuts at: 3284 4949 3284 Size: 1665 4453 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AlwNI CAG_nnn'CTG Cuts at: 2243 2823 3861 5433 2243 Size: 580 1038 1572 2928 Fragments arranged by size: 2928 1572 1038 580 ApaI G_GGCC'C Cuts at: 2495 2495 Size: 6118 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 3427 3653 3805 5914 1493 Size: 1934 226 152 2109 1697 Fragments arranged by size: 2109 1934 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 3224 3242 3443 1870 Size: 73 1281 18 201 4545 Fragments arranged by size: 4545 1281 201 73 18 BamHI G'GATC_C Cuts at: 2944 3448 3815 2944 Size: 504 367 5247 Fragments arranged by size: 5247 504 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3038 3439 4894 618 Size: 325 217 627 1251 401 1455 1842 Fragments arranged by size: 1842 1455 1251 627 401 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2070 2495 2738 3294 3437 1391 Size: 631 48 425 243 556 143 4072 Fragments arranged by size: 4072 631 556 425 243 143 48 BbeI G_GCGC'C Cuts at: 3042 3042 Size: 6118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4309 4343 1903 Size: 34 50 34 2288 34 3678 Fragments arranged by size: 3678 2288 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4010 5220 4010 Size: 1210 4908 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BfrBI ATG'CAT Cuts at: 2512 2512 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2855 4729 136 Size: 107 122 71 631 605 1183 1874 1525 Fragments arranged by size: 1874 1525 1183 631 605 122 107 71 BglII A'GATC_T Cuts at: 2889 6113 2889 Size: 3224 2894 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 2495 2574 4179 5335 1790 Size: 705 79 1605 1156 2573 Fragments arranged by size: 2573 1605 1156 705 79 BmgBI CAC'GTC Cuts at: 1318 3196 1318 Size: 1878 4240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3849 4807 442 Size: 416 53 83 399 24 2432 958 1753 Fragments arranged by size: 2432 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3296 3296 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2178 2537 3159 4778 1239 Size: 815 124 359 622 1619 2579 Fragments arranged by size: 2579 1619 815 622 359 124 Bpu10I CC'TnA_GC Cuts at: 2737 2737 Size: 6118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3278 4243 5108 5406 3278 Size: 965 865 298 3990 Fragments arranged by size: 3990 965 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4781 882 Size: 617 3282 2219 Fragments arranged by size: 3282 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 2433 6112 2433 Size: 3679 2439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2970 3039 3925 4307 Size: 53 83 186 936 1437 69 886 382 Cuts at: 4307 4942 275 Size: 635 1451 Fragments arranged by size: 1451 1437 936 886 635 382 186 83 69 53 BsaWI w'CCGG_w Cuts at: 3284 4551 4949 5223 5370 5781 3284 Size: 1267 398 274 147 411 3621 Fragments arranged by size: 3621 1267 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3056 3086 1178 Size: 30 165 30 1653 30 4210 Fragments arranged by size: 4210 1653 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3064 3173 1186 Size: 1878 109 4131 Fragments arranged by size: 4131 1878 109 BseYI C'CCAG_C Cuts at: 2203 3025 5321 5817 2203 Size: 822 2296 496 2504 Fragments arranged by size: 2504 2296 822 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5545 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2070 2738 3165 3294 3339 4179 4264 5335 2070 Size: 668 427 129 45 840 85 1071 2853 Fragments arranged by size: 2853 1071 840 668 427 129 85 45 BsmI GAATG_Cn' Cuts at: 2662 3629 3722 2662 Size: 967 93 5058 Fragments arranged by size: 5058 967 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2070 2495 2574 2738 3165 3294 Size: 399 232 48 425 79 164 427 129 Cuts at: 3294 3339 3437 4179 4264 5335 1391 Size: 45 98 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 427 425 399 232 164 129 98 85 79 48 45 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 3384 4007 5737 3384 Size: 623 1730 3765 Fragments arranged by size: 3765 1730 623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3473 844 Size: 2629 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2514 4613 4787 65 Size: 1183 1266 2099 174 1396 Fragments arranged by size: 2099 1396 1266 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2375 3284 4762 4949 1700 Size: 110 565 909 1478 187 2869 Fragments arranged by size: 2869 1478 909 565 187 110 BsrGI T'GTAC_A Cuts at: 96 2478 96 Size: 2382 3736 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 2519 1184 Size: 1335 4783 BstYI r'GATC_y Cuts at: 1972 2889 2944 3448 3815 4200 4217 5669 5755 Size: 917 55 504 367 385 17 1452 86 Cuts at: 5755 5767 6113 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 917 504 385 367 346 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2163 2264 513 Size: 552 1098 101 4367 Fragments arranged by size: 4367 1098 552 101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2035 2656 487 Size: 683 56 809 621 3949 Fragments arranged by size: 3949 809 683 621 56 BtsI GCAGTG_nn' Cuts at: 2290 3634 4428 4448 2290 Size: 1344 794 20 3960 Fragments arranged by size: 3960 1344 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DraIII CAC_nnn'GTG Cuts at: 2547 2547 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2377 3096 3565 Size: 54 1425 50 275 18 547 719 469 Cuts at: 3565 4454 6083 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 719 547 469 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2241 2503 4048 1353 Size: 888 262 1545 3423 Fragments arranged by size: 3423 1545 888 262 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2068 2736 3292 2068 Size: 668 556 4894 Fragments arranged by size: 4894 668 556 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2178 2265 2523 2537 2854 3159 Size: 393 422 124 87 258 14 317 305 Cuts at: 3159 4181 4778 5565 5833 1239 Size: 1022 597 787 268 1524 Fragments arranged by size: 1524 1022 787 597 422 393 317 305 268 258 124 87 14 EcoNI CCTnn'n_nnAGG Cuts at: 2630 2630 Size: 6118 EcoO109I rG'GnC_Cy Cuts at: 2076 2491 2492 2617 2076 Size: 415 1 125 5577 Fragments arranged by size: 5577 415 125 1 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 EcoRV GAT'ATC Cuts at: 2911 2911 Size: 6118 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3421 3421 Size: 6118 FspI TGC'GCA Cuts at: 2402 4624 2402 Size: 2222 3896 HaeII r_GCGC'y Cuts at: 1678 3042 5265 1678 Size: 1364 2223 2531 Fragments arranged by size: 2531 2223 1364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2974 3006 3056 Size: 32 135 32 437 32 933 32 50 Cuts at: 3056 3088 3138 3170 4759 4791 4833 4865 1373 Size: 32 50 32 1589 32 42 32 2626 Fragments arranged by size: 2626 1589 933 437 135 50 50 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3708 669 Size: 988 281 1524 246 3079 Fragments arranged by size: 3079 1524 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6118 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KasI G'GCGC_C Cuts at: 3038 3038 Size: 6118 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5232 5416 1305 Size: 3927 184 2007 Fragments arranged by size: 3927 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4296 Fragments arranged by size: 4296 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 2729 4076 4435 4594 518 Size: 648 1419 144 1347 359 159 2042 Fragments arranged by size: 2042 1419 1347 648 359 159 144 MspA1I CmG'CkG Cuts at: 1606 2165 3270 3534 4211 5359 5604 1606 Size: 559 1105 264 677 1148 245 2120 Fragments arranged by size: 2120 1148 1105 677 559 264 245 NarI GG'CG_CC Cuts at: 3039 3039 Size: 6118 NcoI C'CATG_G Cuts at: 513 1065 2264 513 Size: 552 1199 4367 Fragments arranged by size: 4367 1199 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NsiI A_TGCA'T Cuts at: 2514 2514 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 2516 2777 3131 3476 5021 1132 Size: 97 1287 261 354 345 1545 2229 Fragments arranged by size: 2229 1545 1287 354 345 261 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6118 PfoI T'CCnGG_A Cuts at: 2553 2553 Size: 6118 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2076 2617 2076 Size: 541 5577 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PspOMI G'GGCC_C Cuts at: 2491 2491 Size: 6118 PspXI vC'TCGA_Gb Cuts at: 3224 3242 3224 Size: 18 6100 PstI C_TGCA'G Cuts at: 830 2122 3036 3321 3470 830 Size: 1292 914 285 149 3478 Fragments arranged by size: 3478 1292 914 285 149 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 SacI G_AGCT'C Cuts at: 2070 2738 3294 2070 Size: 668 556 4894 Fragments arranged by size: 4894 668 556 SacII CC_GC'GG Cuts at: 2166 2166 Size: 6118 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SbfI CC_TGCA'GG Cuts at: 3036 3470 3036 Size: 434 5684 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SfcI C'TryA_G Cuts at: 826 1045 2118 2638 3032 3317 3466 4601 5282 Size: 219 1073 520 394 285 149 1135 681 Cuts at: 5282 5473 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1073 681 520 394 285 219 191 149 SfoI GGC'GCC Cuts at: 3040 3040 Size: 6118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 3284 1700 Size: 1584 4534 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3224 3242 3293 4222 5123 5385 Size: 197 926 1281 18 51 929 901 262 Cuts at: 5385 820 Size: 1553 Fragments arranged by size: 1553 1281 929 926 901 262 197 51 18 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3131 3476 1132 Size: 1999 345 3774 Fragments arranged by size: 3774 1999 345 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 2636 1517 Size: 1119 4999 StyI C'CwwG_G Cuts at: 513 1065 2264 2574 2661 3114 3557 513 Size: 552 1199 310 87 453 443 3074 Fragments arranged by size: 3074 1199 552 453 443 310 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4145 4162 4315 4500 4945 325 Size: 1255 2565 17 153 185 445 1498 Fragments arranged by size: 2565 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2272 2478 4364 Size: 274 80 33 51 1443 295 206 1886 Cuts at: 4364 6076 96 Size: 1712 138 Fragments arranged by size: 1886 1712 1443 295 274 206 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2379 2411 2877 2900 2909 2932 6078 6110 2379 Size: 32 466 23 9 23 3146 32 2387 Fragments arranged by size: 3146 2387 466 32 32 23 23 9 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6118 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 3224 3242 1943 Size: 1281 18 4819 Fragments arranged by size: 4819 1281 18 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 3380 4247 3380 Size: 867 5251 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2971 3926 276 Size: 53 83 186 936 1437 955 2468 Fragments arranged by size: 2468 1437 955 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I FseI FspAI NaeI NgoMIV NruI PacI PmlI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI