(Circular) (Six-base) MAPSORT of: pFN21AB4894.seq Check: 5080 from: 1 to: 5926 pFN21AB4894 5926 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3989 5373 5641 1632 Size: 2357 1384 268 1917 Fragments arranged by size: 2357 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 2236 2639 2833 4825 5802 1203 Size: 1033 403 194 1992 977 1327 Fragments arranged by size: 1992 1327 1033 977 403 194 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 2379 1166 Size: 1213 4713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 3669 5241 3669 Size: 1572 4354 ApaI G_GGCC'C Cuts at: 2753 2753 Size: 5926 ApaLI G'TGCA_C Cuts at: 2174 3983 5139 2174 Size: 1809 1156 2961 Fragments arranged by size: 2961 1809 1156 ApoI r'AATT_y Cuts at: 1493 3235 3461 3613 5722 1493 Size: 1742 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2203 2511 2655 2691 2753 3251 1870 Size: 73 260 308 144 36 62 498 4545 Fragments arranged by size: 4545 498 308 260 144 73 62 36 BamHI G'GATC_C Cuts at: 2790 3178 3256 3623 2790 Size: 388 78 367 5093 Fragments arranged by size: 5093 388 367 78 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2272 2310 2494 2696 Size: 325 217 627 351 134 38 184 202 Cuts at: 2696 2912 3247 4702 618 Size: 216 335 1455 1842 Fragments arranged by size: 1842 1455 627 351 335 325 217 216 202 184 134 38 BanII G_rGCy'C Cuts at: 1391 2070 2204 2512 2753 3077 3245 1391 Size: 679 134 308 241 324 168 4072 Fragments arranged by size: 4072 679 324 308 241 168 134 BbeI G_GCGC'C Cuts at: 2276 2314 2700 2276 Size: 38 386 5502 Fragments arranged by size: 5502 386 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4955 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4117 4151 1903 Size: 34 50 34 2096 34 3678 Fragments arranged by size: 3678 2096 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2308 2492 2736 Size: 107 122 71 631 605 636 184 244 Cuts at: 2736 3007 3114 4537 136 Size: 271 107 1423 1525 Fragments arranged by size: 1525 1423 636 631 605 271 244 184 122 107 107 71 BglII A'GATC_T Cuts at: 5921 5921 Size: 5926 BlpI GC'TnA_GC Cuts at: 3343 3343 Size: 5926 Bme1580I G_kGCm'C Cuts at: 1790 2143 2178 2753 2785 3987 5143 1790 Size: 353 35 575 32 1202 1156 2573 Fragments arranged by size: 2573 1202 1156 575 353 35 32 BmgBI CAC'GTC Cuts at: 1318 2642 1318 Size: 1324 4602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2792 3657 4615 Size: 416 53 83 399 24 1375 865 958 Cuts at: 4615 442 Size: 1753 Fragments arranged by size: 1753 1375 958 865 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2912 2932 3004 4586 1239 Size: 893 780 20 72 1582 2579 Fragments arranged by size: 2579 1582 893 780 72 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2484 2834 4051 4916 5214 2484 Size: 350 1217 865 298 3196 Fragments arranged by size: 3196 1217 865 350 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2282 4589 882 Size: 617 783 2307 2219 Fragments arranged by size: 2307 2219 783 617 BsaAI yAC'GTr Cuts at: 493 1204 2173 2836 3717 493 Size: 711 969 663 881 2702 Fragments arranged by size: 2702 969 881 711 663 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2273 2311 2697 Size: 53 83 186 936 528 212 38 386 Cuts at: 2697 3733 4115 4750 275 Size: 1036 382 635 1451 Fragments arranged by size: 1451 1036 936 635 528 386 382 212 186 83 53 38 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2287 2317 2618 2648 1178 Size: 30 165 30 884 30 301 30 4456 Fragments arranged by size: 4456 884 301 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3009 3068 3188 1186 Size: 1823 59 120 3924 Fragments arranged by size: 3924 1823 120 59 BseYI C'CCAG_C Cuts at: 2425 2989 5129 5625 2425 Size: 564 2140 496 2726 Fragments arranged by size: 2726 2140 564 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2057 2393 2690 2801 1163 Size: 573 321 336 297 111 4288 Fragments arranged by size: 4288 573 336 321 297 111 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 2578 3376 4137 5165 1540 Size: 275 186 54 523 798 761 1028 2301 Fragments arranged by size: 2301 1028 798 761 523 275 186 54 BsiHKAI G_wGCw'C Cuts at: 2178 2210 3987 4072 5143 2178 Size: 32 1777 85 1071 2961 Fragments arranged by size: 2961 1777 1071 85 32 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2143 2178 2204 2210 2512 2753 Size: 399 280 73 35 26 6 302 241 Cuts at: 2753 2785 3077 3245 3987 4072 5143 1391 Size: 32 292 168 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 302 292 280 241 168 85 73 35 32 26 6 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3281 844 Size: 2437 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3007 3813 1394 Size: 429 1184 806 3507 Fragments arranged by size: 3507 1184 806 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2412 2743 4570 4757 1700 Size: 110 602 331 1827 187 2869 Fragments arranged by size: 2869 1827 602 331 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 2152 2154 1925 Size: 227 2 5697 Fragments arranged by size: 5697 227 2 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 3222 794 Size: 2428 3498 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstXI CCAn_nnnn'nTGG Cuts at: 2269 2269 Size: 5926 BstYI r'GATC_y Cuts at: 1972 2790 3178 3256 3623 4008 4025 5477 5563 Size: 818 388 78 367 385 17 1452 86 Cuts at: 5563 5575 5921 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 818 388 385 367 346 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2144 3012 2144 Size: 868 5058 BtgI C'CryG_G Cuts at: 513 1065 2055 2091 2545 2664 513 Size: 552 990 36 454 119 3775 Fragments arranged by size: 3775 990 552 454 119 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 2724 2783 2818 3442 4236 4256 2724 Size: 59 35 624 794 20 4394 Fragments arranged by size: 4394 794 624 59 35 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2565 2724 Size: 54 1425 50 275 18 253 482 159 Cuts at: 2724 3373 4262 5891 8 Size: 649 889 1629 43 Fragments arranged by size: 1629 1425 889 649 482 275 253 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3856 1353 Size: 2503 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2912 2932 3004 3989 4586 5373 Size: 393 500 780 20 72 985 597 787 Cuts at: 5373 5641 1239 Size: 268 1524 Fragments arranged by size: 1524 985 787 780 597 500 393 268 72 20 EcoO109I rG'GnC_Cy Cuts at: 2422 2750 2804 2994 3094 2422 Size: 328 54 190 100 5254 Fragments arranged by size: 5254 328 190 100 54 EcoRI G'AATT_C Cuts at: 3235 3613 3235 Size: 378 5548 EcoRV GAT'ATC Cuts at: 2866 2866 Size: 5926 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 2127 2276 2314 2700 2768 5073 1678 Size: 449 149 38 386 68 2305 2531 Fragments arranged by size: 2531 2305 449 386 149 68 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2287 2319 2589 2618 2621 Size: 32 135 32 715 32 270 29 3 Cuts at: 2621 2650 4567 4599 4641 4673 1373 Size: 29 1917 32 42 32 2626 Fragments arranged by size: 2626 1917 715 270 135 42 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KasI G'GCGC_C Cuts at: 2272 2310 2696 2272 Size: 38 386 5502 Fragments arranged by size: 5502 386 38 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 2236 5802 2236 Size: 3566 2360 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2022 5040 5224 1305 Size: 717 3018 184 2007 Fragments arranged by size: 3018 2007 717 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2726 10 Size: 54 1425 343 894 3210 Fragments arranged by size: 3210 1425 894 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2379 3884 4243 4402 518 Size: 648 1213 1505 359 159 2042 Fragments arranged by size: 2042 1505 1213 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2057 2338 2761 3342 4019 5167 5412 1606 Size: 451 281 423 581 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 581 451 423 281 245 NarI GG'CG_CC Cuts at: 2273 2311 2697 2273 Size: 38 386 5502 Fragments arranged by size: 5502 386 38 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5374 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 3284 4829 1132 Size: 97 2055 1545 2229 Fragments arranged by size: 2229 2055 1545 97 PasI CC'CwG_GG Cuts at: 1348 2418 1348 Size: 1070 4856 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 2092 1108 Size: 984 4942 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PmlI CAC'GTG Cuts at: 2173 2836 2173 Size: 663 5263 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2309 2341 3995 4027 5508 5540 2309 Size: 32 1654 32 1481 32 2695 Fragments arranged by size: 2695 1654 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2422 2804 3094 2422 Size: 382 290 5254 Fragments arranged by size: 5254 382 290 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PspOMI G'GGCC_C Cuts at: 2749 2749 Size: 5926 PspXI vC'TCGA_Gb Cuts at: 2203 2203 Size: 5926 PstI C_TGCA'G Cuts at: 830 3278 830 Size: 2448 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2338 2338 Size: 5926 SacII CC_GC'GG Cuts at: 2058 2058 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SanDI GG'GwC_CC Cuts at: 2422 2422 Size: 5926 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 3029 4174 5886 3029 Size: 1145 1712 3069 Fragments arranged by size: 3069 1712 1145 SfcI C'TryA_G Cuts at: 826 1045 2227 3274 4409 5090 5281 826 Size: 219 1182 1047 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 1047 681 219 191 SfoI GGC'GCC Cuts at: 2274 2312 2698 2274 Size: 38 386 5502 Fragments arranged by size: 5502 386 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 2755 3253 1872 Size: 883 498 4545 Fragments arranged by size: 4545 883 498 SmlI C'TyrA_G Cuts at: 820 1017 1943 2203 2499 2849 4030 4931 5193 Size: 197 926 260 296 350 1181 901 262 Cuts at: 5193 820 Size: 1553 Fragments arranged by size: 1553 1181 926 901 350 296 262 260 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 3284 1132 Size: 2152 3774 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 3063 3365 513 Size: 552 1998 302 3074 Fragments arranged by size: 3074 1998 552 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2126 2198 3953 3970 4123 4308 4753 Size: 1255 546 72 1755 17 153 185 445 Cuts at: 4753 325 Size: 1498 Fragments arranged by size: 1755 1498 1255 546 445 185 153 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3027 3165 4172 Size: 274 80 33 51 1443 1050 138 1007 Cuts at: 4172 5884 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1050 1007 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2775 2807 5886 5918 2775 Size: 32 3079 32 2783 Fragments arranged by size: 3079 2783 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2185 1316 Size: 869 5057 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2887 2974 2887 Size: 87 5839 XhoI C'TCGA_G Cuts at: 1943 2203 1943 Size: 260 5666 XmaI C'CCGG_G Cuts at: 1870 2753 3251 1870 Size: 883 498 4545 Fragments arranged by size: 4545 883 498 XmnI GAAnn'nnTTC Cuts at: 2027 2447 4055 2027 Size: 420 1608 3898 Fragments arranged by size: 3898 1608 420 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI