Restriction map for
pFN21AB4894
(Linear) (Six-base) MAPSORT of: KIBB4894.seq Check: 4931 from: 1 to: 1221
KIBB4894.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AflIII A'CryG_T
Cuts at: 0 231 634 828 1221
Size: 231 403 194 393
Fragments arranged by size:
403 393 231 194
AleI CACnn'nnGTG
Cuts at: 0 374 1221
Size: 374 847
ApaI G_GGCC'C
Cuts at: 0 748 1221
Size: 748 473
ApaLI G'TGCA_C
Cuts at: 0 169 1221
Size: 169 1052
AvaI C'yCGr_G
Cuts at: 0 198 506 650 686 748 1221
Size: 198 308 144 36 62 473
Fragments arranged by size:
473 308 198 144 62 36
BamHI G'GATC_C
Cuts at: 0 785 1173 1221
Size: 785 388 48
Fragments arranged by size:
785 388 48
BanI G'GyrC_C
Cuts at: 0 133 267 305 489 691 907 1221
Size: 133 134 38 184 202 216 314
Fragments arranged by size:
314 216 202 184 134 133 38
BanII G_rGCy'C
Cuts at: 0 65 199 507 748 1072 1221
Size: 65 134 308 241 324 149
Fragments arranged by size:
324 308 241 149 134 65
BbeI G_GCGC'C
Cuts at: 0 271 309 695 1221
Size: 271 38 386 526
Fragments arranged by size:
526 386 271 38
BglI GCCn_nnn'nGGC
Cuts at: 0 303 487 731 1002 1109 1221
Size: 303 184 244 271 107 112
Fragments arranged by size:
303 271 244 184 112 107
Bme1580I G_kGCm'C
Cuts at: 0 138 173 748 780 1221
Size: 138 35 575 32 441
Fragments arranged by size:
575 441 138 35 32
BmgBI CAC'GTC
Cuts at: 0 637 1221
Size: 637 584
BmrI ACTGGGnnnn_n'
Cuts at: 0 787 1221
Size: 787 434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 907 927 999 1221
Size: 127 780 20 72 222
Fragments arranged by size:
780 222 127 72 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 829 1221
Size: 479 350 392
Fragments arranged by size:
479 392 350
BsaI GGTCTCn'nnnn_
Cuts at: 0 277 1221
Size: 277 944
BsaAI yAC'GTr
Cuts at: 0 168 831 1221
Size: 168 663 390
Fragments arranged by size:
663 390 168
BsaHI Gr'CG_yC
Cuts at: 0 56 268 306 692 1221
Size: 56 212 38 386 529
Fragments arranged by size:
529 386 212 56 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 282 312 613 643 1221
Size: 282 30 301 30 578
Fragments arranged by size:
578 301 282 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1004 1063 1183 1221
Size: 1004 59 120 38
Fragments arranged by size:
1004 120 59 38
BseYI C'CCAG_C
Cuts at: 0 420 984 1221
Size: 420 564 237
Fragments arranged by size:
564 420 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 388 685 796 1221
Size: 52 336 297 111 425
Fragments arranged by size:
425 336 297 111 52
BsiEI CG_ry'CG
Cuts at: 0 50 573 1221
Size: 50 523 648
Fragments arranged by size:
648 523 50
BsiHKAI G_wGCw'C
Cuts at: 0 173 205 1221
Size: 173 32 1016
Fragments arranged by size:
1016 173 32
Bsp1286I G_dGCh'C
Cuts at: 0 65 138 173 199 205 507 748 780
Size: 65 73 35 26 6 302 241 32
Cuts at: 780 1072 1221
Size: 292 149
Fragments arranged by size:
302 292 241 149 73 65 35 32
26 6
BsrBI CCG'CTC
Cuts at: 0 1002 1221
Size: 1002 219
BsrFI r'CCGG_y
Cuts at: 0 407 738 1221
Size: 407 331 483
Fragments arranged by size:
483 407 331
BssHII G'CGCG_C
Cuts at: 0 147 149 1221
Size: 147 2 1072
Fragments arranged by size:
1072 147 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1217 1221
Size: 1217 4
BstXI CCAn_nnnn'nTGG
Cuts at: 0 264 1221
Size: 264 957
BstYI r'GATC_y
Cuts at: 0 785 1173 1221
Size: 785 388 48
Fragments arranged by size:
785 388 48
Bsu36I CC'TnA_GG
Cuts at: 0 139 1007 1221
Size: 139 868 214
Fragments arranged by size:
868 214 139
BtgI C'CryG_G
Cuts at: 0 50 86 540 659 1221
Size: 50 36 454 119 562
Fragments arranged by size:
562 454 119 50 36
BtsI GCAGTG_nn'
Cuts at: 0 719 778 813 1221
Size: 719 59 35 408
Fragments arranged by size:
719 408 59 35
EaeI y'GGCC_r
Cuts at: 0 78 560 719 1221
Size: 78 482 159 502
Fragments arranged by size:
502 482 159 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 907 927 999 1221
Size: 127 780 20 72 222
Fragments arranged by size:
780 222 127 72 20
EcoO109I rG'GnC_Cy
Cuts at: 0 417 745 799 989 1089 1221
Size: 417 328 54 190 100 132
Fragments arranged by size:
417 328 190 132 100 54
EcoRV GAT'ATC
Cuts at: 0 861 1221
Size: 861 360
HaeII r_GCGC'y
Cuts at: 0 122 271 309 695 763 1221
Size: 122 149 38 386 68 458
Fragments arranged by size:
458 386 149 122 68 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 282 314 584 613 616 645 1221
Size: 282 32 270 29 3 29 576
Fragments arranged by size:
576 282 270 32 29 29 3
KasI G'GCGC_C
Cuts at: 0 267 305 691 1221
Size: 267 38 386 530
Fragments arranged by size:
530 386 267 38
MluI A'CGCG_T
Cuts at: 0 231 1221
Size: 231 990
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 1221
Size: 17 1204
MscI TGG'CCA
Cuts at: 0 721 1221
Size: 721 500
MslI CAynn'nnrTG
Cuts at: 0 374 1221
Size: 374 847
MspA1I CmG'CkG
Cuts at: 0 52 333 756 1221
Size: 52 281 423 465
Fragments arranged by size:
465 423 281 52
NarI GG'CG_CC
Cuts at: 0 268 306 692 1221
Size: 268 38 386 529
Fragments arranged by size:
529 386 268 38
PasI CC'CwG_GG
Cuts at: 0 413 1221
Size: 413 808
PflMI CCAn_nnn'nTGG
Cuts at: 0 87 1221
Size: 87 1134
PmlI CAC'GTG
Cuts at: 0 168 831 1221
Size: 168 663 390
Fragments arranged by size:
663 390 168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 1221
Size: 304 32 885
Fragments arranged by size:
885 304 32
PpuMI rG'GwC_Cy
Cuts at: 0 417 799 1089 1221
Size: 417 382 290 132
Fragments arranged by size:
417 382 290 132
PspOMI G'GGCC_C
Cuts at: 0 744 1221
Size: 744 477
PspXI vC'TCGA_Gb
Cuts at: 0 198 1221
Size: 198 1023
PvuII CAG'CTG
Cuts at: 0 333 1221
Size: 333 888
SacII CC_GC'GG
Cuts at: 0 53 1221
Size: 53 1168
SanDI GG'GwC_CC
Cuts at: 0 417 1221
Size: 417 804
ScaI AGT'ACT
Cuts at: 0 1024 1221
Size: 1024 197
SfcI C'TryA_G
Cuts at: 0 222 1221
Size: 222 999
SfoI GGC'GCC
Cuts at: 0 269 307 693 1221
Size: 269 38 386 528
Fragments arranged by size:
528 386 269 38
SmaI CCC'GGG
Cuts at: 0 750 1221
Size: 750 471
SmlI C'TyrA_G
Cuts at: 0 198 494 844 1221
Size: 198 296 350 377
Fragments arranged by size:
377 350 296 198
StyI C'CwwG_G
Cuts at: 0 1058 1221
Size: 1058 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 121 193 1221
Size: 121 72 1028
Fragments arranged by size:
1028 121 72
TatI w'GTAC_w
Cuts at: 0 1022 1160 1221
Size: 1022 138 61
Fragments arranged by size:
1022 138 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 770 802 1221
Size: 770 32 419
Fragments arranged by size:
770 419 32
Tth111I GACn'n_nGTC
Cuts at: 0 180 1221
Size: 180 1041
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 882 969 1221
Size: 882 87 252
Fragments arranged by size:
882 252 87
XhoI C'TCGA_G
Cuts at: 0 198 1221
Size: 198 1023
XmaI C'CCGG_G
Cuts at: 0 748 1221
Size: 748 473
XmnI GAAnn'nnTTC
Cuts at: 0 22 442 1221
Size: 22 420 779
Fragments arranged by size:
779 420 22
Enzymes that do cut:
AflIII AleI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrBI BsrFI BssHII BstAPI BstXI BstYI Bsu36I
BtgI BtsI EaeI Eco57MI EcoO109I EcoRV HaeII Hin4I
KasI MluI MmeI MscI MslI MspA1I NarI PasI
PflMI PmlI PpiI PpuMI PspOMI PspXI PvuII SacII
SanDI ScaI SfcI SfoI SmaI SmlI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmtI BplI Bpu10I BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PshAI PsiI
PsrI PstI PvuI RsrII SacI SalI SapI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI ZraI