(Circular) (Six-base) MAPSORT of: pFN21AB4708.seq Check: 4932 from: 1 to: 6037 pFN21AB4708 6037 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3101 3101 Size: 6037 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3847 278 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2565 3380 1937 Size: 628 815 4594 Fragments arranged by size: 4594 815 628 Acc65I G'GTAC_C Cuts at: 3358 3358 Size: 6037 AclI AA'CG_TT Cuts at: 1563 4164 4537 1563 Size: 2601 373 3063 Fragments arranged by size: 3063 2601 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2050 3314 4100 5484 5752 1632 Size: 418 1264 786 1384 268 1917 Fragments arranged by size: 1917 1384 1264 786 418 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5840 AflIII A'CryG_T Cuts at: 1203 2394 2715 4936 5913 1203 Size: 1191 321 2221 977 1327 Fragments arranged by size: 2221 1327 1191 977 321 AgeI A'CCGG_T Cuts at: 4868 4868 Size: 6037 AhdI GACnn_n'nnGTC Cuts at: 4766 4766 Size: 6037 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6037 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6037 AlwNI CAG_nnn'CTG Cuts at: 3780 5352 3780 Size: 1572 4465 ApaI G_GGCC'C Cuts at: 2350 3271 2350 Size: 921 5116 ApaLI G'TGCA_C Cuts at: 4094 5250 4094 Size: 1156 4881 ApoI r'AATT_y Cuts at: 1493 2243 2600 3346 3572 3724 5833 1493 Size: 750 357 746 226 152 2109 1697 Fragments arranged by size: 2109 1697 750 746 357 226 152 AseI AT'TA_AT Cuts at: 160 1032 4591 160 Size: 872 3559 1606 Fragments arranged by size: 3559 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 AvaI C'yCGr_G Cuts at: 1870 1943 2164 3362 1870 Size: 73 221 1198 4545 Fragments arranged by size: 4545 1198 221 73 BamHI G'GATC_C Cuts at: 2692 3367 3734 2692 Size: 675 367 4995 Fragments arranged by size: 4995 675 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2624 3358 4813 618 Size: 325 217 627 269 568 734 1455 1842 Fragments arranged by size: 1842 1455 734 627 568 325 269 217 BanII G_rGCy'C Cuts at: 1391 2301 2350 2962 3271 3356 1391 Size: 910 49 612 309 85 4072 Fragments arranged by size: 4072 910 612 309 85 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2363 928 Size: 971 464 4602 Fragments arranged by size: 4602 971 464 BbvCI CC'TCA_GC Cuts at: 2364 2364 Size: 6037 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4228 4262 1903 Size: 34 2291 34 3678 Fragments arranged by size: 3678 2291 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3929 5139 3929 Size: 1210 4827 BclI T'GATC_A Cuts at: 1548 1548 Size: 6037 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4648 136 Size: 107 122 71 631 605 2976 1525 Fragments arranged by size: 2976 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6032 6032 Size: 6037 BlpI GC'TnA_GC Cuts at: 3454 3454 Size: 6037 Bme1580I G_kGCm'C Cuts at: 1790 2061 2190 2350 3271 4098 5254 1790 Size: 271 129 160 921 827 1156 2573 Fragments arranged by size: 2573 1156 921 827 271 160 129 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6037 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2890 3768 4726 Size: 416 53 83 399 24 1473 878 958 Cuts at: 4726 442 Size: 1753 Fragments arranged by size: 1753 1473 958 878 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6037 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2217 2453 3011 4697 1239 Size: 978 236 558 1686 2579 Fragments arranged by size: 2579 1686 978 558 236 Bpu10I CC'TnA_GC Cuts at: 2364 2364 Size: 6037 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4162 5027 5325 4162 Size: 865 298 4874 Fragments arranged by size: 4874 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4700 882 Size: 617 3201 2219 Fragments arranged by size: 3201 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2395 2716 3828 493 Size: 711 1191 321 1112 2702 Fragments arranged by size: 2702 1191 1112 711 321 BsaBI GATnn'nnATC Cuts at: 6031 6031 Size: 6037 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3321 3844 4226 4861 Size: 53 83 186 936 1788 523 382 635 Cuts at: 4861 275 Size: 1451 Fragments arranged by size: 1788 1451 936 635 523 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2916 4470 4868 5142 5289 5700 2916 Size: 1554 398 274 147 411 3253 Fragments arranged by size: 3253 1554 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5812 Fragments arranged by size: 5812 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2309 2396 2399 1186 Size: 904 219 87 3 4824 Fragments arranged by size: 4824 904 219 87 3 BseYI C'CCAG_C Cuts at: 2070 2300 5240 5736 2070 Size: 230 2940 496 2371 Fragments arranged by size: 2940 2371 496 230 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2696 1163 Size: 573 960 4504 Fragments arranged by size: 4504 960 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3487 4248 5276 1540 Size: 275 186 1486 761 1028 2301 Fragments arranged by size: 2301 1486 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3159 4098 4183 5254 3159 Size: 939 85 1071 3942 Fragments arranged by size: 3942 1071 939 85 BsmI GAATG_Cn' Cuts at: 3548 3641 3548 Size: 93 5944 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2190 2301 2350 2962 3159 3271 Size: 399 271 129 111 49 612 197 112 Cuts at: 3271 3356 4098 4183 5254 1391 Size: 85 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 612 399 271 197 129 112 111 85 85 49 BspEI T'CCGG_A Cuts at: 5700 5700 Size: 6037 BspHI T'CATG_A Cuts at: 2856 3926 5656 2856 Size: 1070 1730 3237 Fragments arranged by size: 3237 1730 1070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2103 2781 3101 3392 844 Size: 1259 678 320 291 3489 Fragments arranged by size: 3489 1259 678 320 291 BsrBI CCG'CTC Cuts at: 1394 1823 3924 1394 Size: 429 2101 3507 Fragments arranged by size: 3507 2101 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2439 2476 4532 4706 65 Size: 1183 1191 37 2056 174 1396 Fragments arranged by size: 2056 1396 1191 1183 174 37 BsrFI r'CCGG_y Cuts at: 1700 1810 4681 4868 1700 Size: 110 2871 187 2869 Fragments arranged by size: 2871 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6037 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6037 BssSI C'ACGA_G Cuts at: 4097 5109 4097 Size: 1012 5025 BstAPI GCAn_nnn'nTGC Cuts at: 794 2095 2213 794 Size: 1301 118 4618 Fragments arranged by size: 4618 1301 118 BstBI TT'CG_AA Cuts at: 4841 4841 Size: 6037 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6037 BstYI r'GATC_y Cuts at: 1972 2692 3367 3734 4119 4136 5588 5674 5686 Size: 720 675 367 385 17 1452 86 12 Cuts at: 5686 6032 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 720 675 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2351 2351 Size: 6037 BtgI C'CryG_G Cuts at: 513 1065 2004 2311 513 Size: 552 939 307 4239 Fragments arranged by size: 4239 939 552 307 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2410 2633 487 Size: 683 56 1184 223 3891 Fragments arranged by size: 3891 1184 683 223 56 BtsI GCAGTG_nn' Cuts at: 3553 4347 4367 3553 Size: 794 20 5223 Fragments arranged by size: 5223 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6002 DraI TTT'AAA Cuts at: 3341 3341 Size: 6037 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5044 809 Size: 507 3728 1802 Fragments arranged by size: 3728 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2551 2611 3484 Size: 54 1425 50 275 18 721 60 873 Cuts at: 3484 4373 6002 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 873 721 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3484 1537 Size: 275 1672 4090 Fragments arranged by size: 4090 1672 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3170 3967 1353 Size: 1817 797 3423 Fragments arranged by size: 3423 1817 797 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4624 4998 5144 387 Size: 1064 138 3035 374 146 1280 Fragments arranged by size: 3035 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 2217 2453 3011 3314 4100 4697 Size: 393 418 167 236 558 303 786 597 Cuts at: 4697 5484 5752 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 786 597 558 418 393 303 268 236 167 EcoO109I rG'GnC_Cy Cuts at: 2347 3267 3268 3288 2347 Size: 920 1 20 5096 Fragments arranged by size: 5096 920 20 1 EcoRI G'AATT_C Cuts at: 2243 3346 3724 2243 Size: 1103 378 4556 Fragments arranged by size: 4556 1103 378 FspI TGC'GCA Cuts at: 2099 4543 2099 Size: 2444 3593 HaeII r_GCGC'y Cuts at: 1678 5184 1678 Size: 3506 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4678 4710 4752 4784 1373 Size: 32 135 32 3106 32 42 32 2626 Fragments arranged by size: 3106 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3381 3627 669 Size: 988 281 1443 246 3079 Fragments arranged by size: 3079 1443 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6037 HpaI GTT'AAC Cuts at: 3627 3627 Size: 6037 KpnI G_GTAC'C Cuts at: 3362 3362 Size: 6037 MfeI C'AATT_G Cuts at: 3636 3636 Size: 6037 MluI A'CGCG_T Cuts at: 5913 5913 Size: 6037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5151 5335 1305 Size: 3846 184 2007 Fragments arranged by size: 3846 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2553 2613 10 Size: 54 1425 343 721 60 3434 Fragments arranged by size: 3434 1425 721 343 60 54 MslI CAynn'nnrTG Cuts at: 518 1166 2547 2831 2861 3020 3035 3995 4354 Size: 648 1381 284 30 159 15 960 359 Cuts at: 4354 4513 518 Size: 159 2042 Fragments arranged by size: 2042 1381 960 648 359 284 159 159 30 15 MspA1I CmG'CkG Cuts at: 1606 2070 2176 2821 3082 3208 3453 4130 5278 Size: 464 106 645 261 126 245 677 1148 Cuts at: 5278 5523 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 645 464 261 245 245 126 106 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4546 Fragments arranged by size: 4546 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6037 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6037 NotI GC'GGCC_GC Cuts at: 3484 3484 Size: 6037 NspI r_CATG'y Cuts at: 1132 1229 2710 3395 4940 1132 Size: 97 1481 685 1545 2229 Fragments arranged by size: 2229 1545 1481 685 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6037 PciI A'CATG_T Cuts at: 4936 4936 Size: 6037 PflMI CCAn_nnn'nTGG Cuts at: 1108 2640 1108 Size: 1532 4505 PfoI T'CCnGG_A Cuts at: 2988 2988 Size: 6037 PmeI GTTT'AAAC Cuts at: 3341 3341 Size: 6037 PmlI CAC'GTG Cuts at: 2395 2716 2395 Size: 321 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4106 4138 5619 5651 4106 Size: 32 1481 32 4492 Fragments arranged by size: 4492 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3288 3288 Size: 6037 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6037 PsiI TTA'TAA Cuts at: 3607 3607 Size: 6037 PspOMI G'GGCC_C Cuts at: 2346 3267 2346 Size: 921 5116 PstI C_TGCA'G Cuts at: 830 3389 830 Size: 2559 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6037 PvuII CAG'CTG Cuts at: 2070 3082 2070 Size: 1012 5025 SalI G'TCGA_C Cuts at: 1936 3379 1936 Size: 1443 4594 SbfI CC_TGCA'GG Cuts at: 3389 3389 Size: 6037 ScaI AGT'ACT Cuts at: 4285 5997 4285 Size: 1712 4325 SfcI C'TryA_G Cuts at: 826 1045 2566 3385 4520 5201 5392 826 Size: 219 1521 819 1135 681 191 1471 Fragments arranged by size: 1521 1471 1135 819 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6037 SmaI CCC'GGG Cuts at: 1872 2166 3364 1872 Size: 294 1198 4545 Fragments arranged by size: 4545 1198 294 SmlI C'TyrA_G Cuts at: 820 1017 1943 4141 5042 5304 820 Size: 197 926 2198 901 262 1553 Fragments arranged by size: 2198 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3828 493 Size: 3335 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6037 SphI G_CATG'C Cuts at: 1132 3395 1132 Size: 2263 3774 SspI AAT'ATT Cuts at: 5 52 3961 5 Size: 47 3909 2081 Fragments arranged by size: 3909 2081 47 StuI AGG'CCT Cuts at: 1517 3056 1517 Size: 1539 4498 StyI C'CwwG_G Cuts at: 513 1065 2004 3476 513 Size: 552 939 1472 3074 Fragments arranged by size: 3074 1472 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2872 4064 4081 4234 4419 4864 325 Size: 1255 1292 1192 17 153 185 445 1498 Fragments arranged by size: 1498 1292 1255 1192 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4283 5995 96 Size: 274 80 33 51 1443 2306 1712 138 Fragments arranged by size: 2306 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5997 6029 5997 Size: 32 6005 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6037 XbaI T'CTAG_A Cuts at: 3373 3373 Size: 6037 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3097 3097 Size: 6037 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6037 XmaI C'CCGG_G Cuts at: 1870 2164 3362 1870 Size: 294 1198 4545 Fragments arranged by size: 4545 1198 294 XmnI GAAnn'nnTTC Cuts at: 2378 4166 2378 Size: 1788 4249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3845 276 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI