(Circular) (Six-base) MAPSORT of: pFN21AB4659.seq Check: 8026 from: 1 to: 6145 pFN21AB4659 6145 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2840 3955 278 Size: 53 83 186 936 1304 1115 2468 Fragments arranged by size: 2468 1304 1115 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2232 3488 1937 Size: 295 1256 4594 Fragments arranged by size: 4594 1256 295 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2570 4208 5592 5860 1632 Size: 938 1638 1384 268 1917 Fragments arranged by size: 1917 1638 1384 938 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 5044 6021 1203 Size: 3841 977 1327 Fragments arranged by size: 3841 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 3150 3164 1166 Size: 1984 14 4147 Fragments arranged by size: 4147 1984 14 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3086 3118 3086 Size: 32 6113 AlwNI CAG_nnn'CTG Cuts at: 2197 2283 2334 2596 2806 3091 3253 3888 5460 Size: 86 51 262 210 285 162 635 1572 Cuts at: 5460 2197 Size: 2882 Fragments arranged by size: 2882 1572 635 285 262 210 162 86 51 ApaI G_GGCC'C Cuts at: 2087 2136 2461 3022 2087 Size: 49 325 561 5210 Fragments arranged by size: 5210 561 325 49 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 3454 3680 3832 5941 1493 Size: 1961 226 152 2109 1697 Fragments arranged by size: 2109 1961 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2080 2127 2186 2462 3470 1870 Size: 73 119 18 47 59 276 1008 4545 Fragments arranged by size: 4545 1008 276 119 73 59 47 18 BamHI G'GATC_C Cuts at: 3475 3842 3475 Size: 367 5778 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2286 2337 2692 3001 3466 Size: 325 217 627 499 51 355 309 465 Cuts at: 3466 4921 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 499 465 355 325 309 217 51 BanII G_rGCy'C Cuts at: 1391 2040 2087 2116 2136 2461 2586 2922 2998 Size: 649 47 29 20 325 125 336 76 Cuts at: 2998 3022 3464 1391 Size: 24 442 4072 Fragments arranged by size: 4072 649 442 336 325 125 76 47 29 24 20 BbeI G_GCGC'C Cuts at: 2290 2341 2290 Size: 51 6094 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2558 2961 3045 3311 928 Size: 971 659 403 84 266 3762 Fragments arranged by size: 3762 971 659 403 266 84 BbvCI CC'TCA_GC Cuts at: 2902 2902 Size: 6145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4336 4370 1903 Size: 34 2399 34 3678 Fragments arranged by size: 3678 2399 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2357 2690 4756 Size: 107 122 71 631 605 685 333 2066 Cuts at: 4756 136 Size: 1525 Fragments arranged by size: 2066 1525 685 631 605 333 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 2087 2136 2461 3022 4206 5362 1790 Size: 297 49 325 561 1184 1156 2573 Fragments arranged by size: 2573 1184 1156 561 325 297 49 BmgBI CAC'GTC Cuts at: 1318 2776 1318 Size: 1458 4687 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3876 4834 442 Size: 416 53 83 399 24 2459 958 1753 Fragments arranged by size: 2459 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2130 3012 2130 Size: 882 5263 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2891 2936 3275 3355 3404 4805 1239 Size: 986 666 45 339 80 49 1401 2579 Fragments arranged by size: 2579 1401 986 666 339 80 49 45 Bpu10I CC'TnA_GC Cuts at: 2902 2997 2902 Size: 95 6050 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2024 3251 4270 5135 5433 2024 Size: 1227 1019 865 298 2736 Fragments arranged by size: 2736 1227 1019 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3367 3936 493 Size: 711 2163 569 2702 Fragments arranged by size: 2702 2163 711 569 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2287 2338 2837 Size: 53 83 186 936 537 217 51 499 Cuts at: 2837 3952 4334 4969 275 Size: 1115 382 635 1451 Fragments arranged by size: 1451 1115 936 635 537 499 382 217 186 83 53 51 BsaWI w'CCGG_w Cuts at: 4578 4976 5250 5397 5808 4578 Size: 398 274 147 411 4915 Fragments arranged by size: 4915 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2609 2639 1178 Size: 30 165 30 1206 30 4684 Fragments arranged by size: 4684 1206 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2171 2617 2861 1186 Size: 922 63 446 244 4470 Fragments arranged by size: 4470 922 446 244 63 BseYI C'CCAG_C Cuts at: 2135 2443 5348 5844 2135 Size: 308 2905 496 2436 Fragments arranged by size: 2905 2436 496 308 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2243 2276 2291 1163 Size: 573 507 33 15 5017 Fragments arranged by size: 5017 573 507 33 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3595 4356 5384 1540 Size: 275 186 1594 761 1028 2301 Fragments arranged by size: 2301 1594 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2040 2565 2586 3171 4206 4291 5362 2040 Size: 525 21 585 1035 85 1071 2823 Fragments arranged by size: 2823 1071 1035 585 525 85 21 BsmI GAATG_Cn' Cuts at: 3656 3749 3656 Size: 93 6052 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2087 2116 2136 2461 2565 2586 Size: 399 250 47 29 20 325 104 21 Cuts at: 2586 2922 2998 3022 3171 3464 4206 4291 5362 Size: 336 76 24 149 293 742 85 1071 Cuts at: 5362 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 336 325 293 250 149 104 85 76 47 29 24 21 20 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2058 2307 3500 844 Size: 1214 249 1193 3489 Fragments arranged by size: 3489 1214 1193 249 BsrBI CCG'CTC Cuts at: 1394 1823 3259 3404 4032 1394 Size: 429 1436 145 628 3507 Fragments arranged by size: 3507 1436 628 429 145 BsrDI GCAATG_nn' Cuts at: 65 1248 2346 4640 4814 65 Size: 1183 1098 2294 174 1396 Fragments arranged by size: 2294 1396 1183 1098 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2555 4789 4976 1700 Size: 110 745 2234 187 2869 Fragments arranged by size: 2869 2234 745 187 110 BsrGI T'GTAC_A Cuts at: 96 3321 96 Size: 3225 2920 BssHII G'CGCG_C Cuts at: 1925 3389 1925 Size: 1464 4681 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 2299 2806 794 Size: 1505 507 4133 Fragments arranged by size: 4133 1505 507 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 2426 2893 1184 Size: 1242 467 4436 Fragments arranged by size: 4436 1242 467 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6145 BstYI r'GATC_y Cuts at: 1972 3475 3842 4227 4244 5696 5782 5794 6140 Size: 1503 367 385 17 1452 86 12 346 Cuts at: 6140 1972 Size: 1977 Fragments arranged by size: 1977 1503 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2658 3075 2658 Size: 417 5728 BtgI C'CryG_G Cuts at: 513 1065 2151 2513 3043 513 Size: 552 1086 362 530 3615 Fragments arranged by size: 3615 1086 552 530 362 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3121 487 Size: 683 56 1895 3511 Fragments arranged by size: 3511 1895 683 56 BtsI GCAGTG_nn' Cuts at: 2250 2611 3196 3661 4455 4475 2250 Size: 361 585 465 794 20 3920 Fragments arranged by size: 3920 794 585 465 361 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 2403 2510 Size: 54 1425 50 275 18 495 78 107 Cuts at: 2510 2577 3592 4481 6110 8 Size: 67 1015 889 1629 43 Fragments arranged by size: 1629 1425 1015 889 495 275 107 78 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3592 1537 Size: 275 1780 4090 Fragments arranged by size: 4090 1780 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3335 4075 1353 Size: 1982 740 3423 Fragments arranged by size: 3423 1982 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 3335 4732 5106 5252 387 Size: 1064 138 441 1305 1397 374 146 1280 Fragments arranged by size: 1397 1305 1280 1064 441 374 146 138 EcoICRI GAG'CTC Cuts at: 2038 2584 2038 Size: 546 5599 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2570 2891 2936 3275 3355 3404 Size: 393 593 345 321 45 339 80 49 Cuts at: 3404 4208 4805 5592 5860 1239 Size: 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 597 593 393 345 339 321 268 80 49 45 EcoNI CCTnn'n_nnAGG Cuts at: 2168 2657 2168 Size: 489 5656 EcoO109I rG'GnC_Cy Cuts at: 2084 2123 2132 2161 2458 2734 2084 Size: 39 9 29 297 276 5495 Fragments arranged by size: 5495 297 276 39 29 9 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FspI TGC'GCA Cuts at: 2474 4651 2474 Size: 2177 3968 HaeII r_GCGC'y Cuts at: 1678 2142 2290 2341 2786 5292 1678 Size: 464 148 51 445 2506 2531 Fragments arranged by size: 2531 2506 464 445 148 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2822 2854 4786 4818 4860 Size: 32 135 32 1250 32 1932 32 42 Cuts at: 4860 4892 1373 Size: 32 2626 Fragments arranged by size: 2626 1932 1250 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3489 3735 669 Size: 988 281 1551 246 3079 Fragments arranged by size: 3079 1551 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6145 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KasI G'GCGC_C Cuts at: 2286 2337 2286 Size: 51 6094 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5259 5443 1305 Size: 3954 184 2007 Fragments arranged by size: 3954 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 10 Size: 54 1425 343 495 3828 Fragments arranged by size: 3828 1425 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2150 2313 3140 3150 3164 4103 Size: 648 843 141 163 827 10 14 939 Cuts at: 4103 4462 4621 518 Size: 359 159 2042 Fragments arranged by size: 2042 939 843 827 648 359 163 159 141 14 10 MspA1I CmG'CkG Cuts at: 1606 2806 2906 3064 3088 3561 4238 5386 5631 Size: 1200 100 158 24 473 677 1148 245 Cuts at: 5631 1606 Size: 2120 Fragments arranged by size: 2120 1200 1148 677 473 245 158 100 24 NarI GG'CG_CC Cuts at: 2287 2338 2287 Size: 51 6094 NcoI C'CATG_G Cuts at: 513 1065 2513 3043 513 Size: 552 1448 530 3615 Fragments arranged by size: 3615 1448 552 530 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 2800 2941 3397 3503 5048 1132 Size: 97 1571 141 456 106 1545 2229 Fragments arranged by size: 2229 1571 1545 456 141 106 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 2283 2334 3253 1108 Size: 1175 51 919 4000 Fragments arranged by size: 4000 1175 919 51 PfoI T'CCnGG_A Cuts at: 2913 3252 3381 2913 Size: 339 129 5677 Fragments arranged by size: 5677 339 129 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PmlI CAC'GTG Cuts at: 3367 3367 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2161 2734 2161 Size: 573 5572 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PspOMI G'GGCC_C Cuts at: 2083 2132 2457 3018 2083 Size: 49 325 561 5210 Fragments arranged by size: 5210 561 325 49 PspXI vC'TCGA_Gb Cuts at: 2127 2186 2127 Size: 59 6086 PstI C_TGCA'G Cuts at: 830 2418 2811 3497 830 Size: 1588 393 686 3478 Fragments arranged by size: 3478 1588 686 393 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2806 2906 3088 2806 Size: 100 182 5863 Fragments arranged by size: 5863 182 100 SacI G_AGCT'C Cuts at: 2040 2586 2040 Size: 546 5599 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SanDI GG'GwC_CC Cuts at: 2734 2734 Size: 6145 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SexAI A'CCwGG_T Cuts at: 2385 2385 Size: 6145 SfcI C'TryA_G Cuts at: 826 1045 2414 2807 3115 3493 4628 5309 5500 Size: 219 1369 393 308 378 1135 681 191 Cuts at: 5500 826 Size: 1471 Fragments arranged by size: 1471 1369 1135 681 393 378 308 219 191 SfoI GGC'GCC Cuts at: 2288 2339 2288 Size: 51 6094 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 2064 3472 1872 Size: 192 1408 4545 Fragments arranged by size: 4545 1408 192 SmlI C'TyrA_G Cuts at: 820 1017 1943 2039 2127 2186 3266 4249 5150 Size: 197 926 96 88 59 1080 983 901 Cuts at: 5150 5412 820 Size: 262 1553 Fragments arranged by size: 1553 1080 983 926 901 262 197 96 88 59 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 2800 3397 3503 1132 Size: 1668 597 106 3774 Fragments arranged by size: 3774 1668 597 106 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 2543 1517 Size: 1026 5119 StyI C'CwwG_G Cuts at: 513 1065 2322 2513 2526 3043 3584 513 Size: 552 1257 191 13 517 541 3074 Fragments arranged by size: 3074 1257 552 541 517 191 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2624 2946 4172 4189 4342 4527 4972 Size: 1255 1044 322 1226 17 153 185 445 Cuts at: 4972 325 Size: 1498 Fragments arranged by size: 1498 1255 1226 1044 445 322 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3321 4391 6103 Size: 274 80 33 51 1443 1344 1070 1712 Cuts at: 6103 96 Size: 138 Fragments arranged by size: 1712 1443 1344 1070 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2009 2041 2302 2334 2555 2587 2609 2641 6105 Size: 32 261 32 221 32 22 32 3464 Cuts at: 6105 6137 2009 Size: 32 2017 Fragments arranged by size: 3464 2017 261 221 32 32 32 32 32 22 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3203 3203 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 2127 2186 1943 Size: 184 59 5902 Fragments arranged by size: 5902 184 59 XmaI C'CCGG_G Cuts at: 1870 2062 3470 1870 Size: 192 1408 4545 Fragments arranged by size: 4545 1408 192 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2838 3953 276 Size: 53 83 186 936 1304 1115 2468 Fragments arranged by size: 2468 1304 1115 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI RsrII SacII SapI SfiI SrfI SwaI