(Circular) (Six-base) MAPSORT of: pFN21AB4651.seq Check: 4078 from: 1 to: 5539 pFN21AB4651 5539 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2094 2094 Size: 5539 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3349 278 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2143 2882 1937 Size: 206 739 4594 Fragments arranged by size: 4594 739 206 Acc65I G'GTAC_C Cuts at: 2860 2860 Size: 5539 AclI AA'CG_TT Cuts at: 1563 3666 4039 1563 Size: 2103 373 3063 Fragments arranged by size: 3063 2103 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3602 4986 5254 1632 Size: 1970 1384 268 1917 Fragments arranged by size: 1970 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5342 AflIII A'CryG_T Cuts at: 1203 4438 5415 1203 Size: 3235 977 1327 Fragments arranged by size: 3235 1327 977 AgeI A'CCGG_T Cuts at: 4370 4370 Size: 5539 AhdI GACnn_n'nnGTC Cuts at: 4268 4268 Size: 5539 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5539 AlwNI CAG_nnn'CTG Cuts at: 2032 3282 4854 2032 Size: 1250 1572 2717 Fragments arranged by size: 2717 1572 1250 ApaLI G'TGCA_C Cuts at: 3596 4752 3596 Size: 1156 4383 ApoI r'AATT_y Cuts at: 1493 2535 2580 2808 2848 3074 3226 5335 1493 Size: 1042 45 228 40 226 152 2109 1697 Fragments arranged by size: 2109 1697 1042 228 226 152 45 40 AseI AT'TA_AT Cuts at: 160 1032 4093 160 Size: 872 3061 1606 Fragments arranged by size: 3061 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 AvaI C'yCGr_G Cuts at: 1870 1943 2864 1870 Size: 73 921 4545 Fragments arranged by size: 4545 921 73 BamHI G'GATC_C Cuts at: 2869 3236 2869 Size: 367 5172 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2860 4315 618 Size: 325 217 627 1073 1455 1842 Fragments arranged by size: 1842 1455 1073 627 325 217 BanII G_rGCy'C Cuts at: 1391 2644 2858 1391 Size: 1253 214 4072 Fragments arranged by size: 4072 1253 214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4568 BbvCI CC'TCA_GC Cuts at: 2589 2589 Size: 5539 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3730 3764 1903 Size: 34 50 34 1709 34 3678 Fragments arranged by size: 3678 1709 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3431 4641 3431 Size: 1210 4329 BclI T'GATC_A Cuts at: 1548 1548 Size: 5539 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2192 4150 136 Size: 107 122 71 631 605 520 1958 1525 Fragments arranged by size: 1958 1525 631 605 520 122 107 71 BglII A'GATC_T Cuts at: 5534 5534 Size: 5539 BlpI GC'TnA_GC Cuts at: 2956 2956 Size: 5539 Bme1580I G_kGCm'C Cuts at: 1790 3600 4756 1790 Size: 1810 1156 2573 Fragments arranged by size: 2573 1810 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5539 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3270 4228 442 Size: 416 53 83 399 24 1853 958 1753 Fragments arranged by size: 1853 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4199 1239 Size: 2960 2579 Bpu10I CC'TnA_GC Cuts at: 2589 2589 Size: 5539 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3664 4529 4827 3664 Size: 865 298 4376 Fragments arranged by size: 4376 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2089 4202 882 Size: 617 590 2113 2219 Fragments arranged by size: 2219 2113 617 590 BsaAI yAC'GTr Cuts at: 493 1204 3330 493 Size: 711 2126 2702 Fragments arranged by size: 2702 2126 711 BsaBI GATnn'nnATC Cuts at: 5533 5533 Size: 5539 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3346 3728 4363 275 Size: 53 83 186 936 1813 382 635 1451 Fragments arranged by size: 1813 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3972 4370 4644 4791 5202 3972 Size: 398 274 147 411 4309 Fragments arranged by size: 4309 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2631 2661 1178 Size: 30 165 30 1228 30 4056 Fragments arranged by size: 4056 1228 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5539 BseYI C'CCAG_C Cuts at: 2046 2151 2484 4742 5238 2046 Size: 105 333 2258 496 2347 Fragments arranged by size: 2347 2258 496 333 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2128 1163 Size: 573 392 4574 Fragments arranged by size: 4574 573 392 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2989 3750 4778 1540 Size: 275 186 988 761 1028 2301 Fragments arranged by size: 2301 1028 988 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3600 3685 4756 3600 Size: 85 1071 4383 Fragments arranged by size: 4383 1071 85 BsmI GAATG_Cn' Cuts at: 3050 3143 3050 Size: 93 5446 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2644 2858 3600 3685 4756 1391 Size: 399 854 214 742 85 1071 2174 Fragments arranged by size: 2174 1071 854 742 399 214 85 BspEI T'CCGG_A Cuts at: 5202 5202 Size: 5539 BspHI T'CATG_A Cuts at: 3428 5158 3428 Size: 1730 3809 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2094 2362 2894 844 Size: 1250 268 532 3489 Fragments arranged by size: 3489 1250 532 268 BsrBI CCG'CTC Cuts at: 1394 1823 3426 1394 Size: 429 1603 3507 Fragments arranged by size: 3507 1603 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2215 4034 4208 65 Size: 1183 967 1819 174 1396 Fragments arranged by size: 1819 1396 1183 967 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4183 4370 1700 Size: 110 2373 187 2869 Fragments arranged by size: 2869 2373 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5539 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5539 BssSI C'ACGA_G Cuts at: 3599 4611 3599 Size: 1012 4527 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5539 BstBI TT'CG_AA Cuts at: 4343 4343 Size: 5539 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5539 BstYI r'GATC_y Cuts at: 1972 2114 2263 2869 3236 3621 3638 5090 5176 Size: 142 149 606 367 385 17 1452 86 Cuts at: 5176 5188 5534 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 606 385 367 346 149 142 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4987 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4800 Fragments arranged by size: 4800 683 56 BtsI GCAGTG_nn' Cuts at: 2364 3055 3849 3869 2364 Size: 691 794 20 4034 Fragments arranged by size: 4034 794 691 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5539 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5504 DraI TTT'AAA Cuts at: 2541 2761 2843 2541 Size: 220 82 5237 Fragments arranged by size: 5237 220 82 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4546 809 Size: 507 3230 1802 Fragments arranged by size: 3230 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504 Size: 54 1425 50 275 18 1156 889 1629 Cuts at: 5504 8 Size: 43 Fragments arranged by size: 1629 1425 1156 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2986 1537 Size: 275 1174 4090 Fragments arranged by size: 4090 1174 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3469 1353 Size: 2116 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4126 4500 4646 387 Size: 1064 138 2537 374 146 1280 Fragments arranged by size: 2537 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3602 4199 4986 5254 1239 Size: 393 1970 597 787 268 1524 Fragments arranged by size: 1970 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2580 2848 3226 2580 Size: 268 378 4893 Fragments arranged by size: 4893 378 268 FspI TGC'GCA Cuts at: 4045 4045 Size: 5539 HaeII r_GCGC'y Cuts at: 1678 4686 1678 Size: 3008 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2401 2433 2551 2583 2600 Size: 32 135 32 829 32 118 32 17 Cuts at: 2600 2631 2632 2663 4180 4212 4254 4286 1373 Size: 31 1 31 1517 32 42 32 2626 Fragments arranged by size: 2626 1517 829 135 118 42 32 32 32 32 32 32 31 31 17 1 HincII GTy'rAC Cuts at: 669 1657 1938 2698 2883 3129 669 Size: 988 281 760 185 246 3079 Fragments arranged by size: 3079 988 760 281 246 185 HindIII A'AGCT_T Cuts at: 748 748 Size: 5539 HpaI GTT'AAC Cuts at: 2698 3129 2698 Size: 431 5108 KpnI G_GTAC'C Cuts at: 2864 2864 Size: 5539 MfeI C'AATT_G Cuts at: 3138 3138 Size: 5539 MluI A'CGCG_T Cuts at: 5415 5415 Size: 5539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4653 4837 1305 Size: 3348 184 2007 Fragments arranged by size: 3348 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3717 Fragments arranged by size: 3717 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2104 3497 3856 4015 518 Size: 648 938 1393 359 159 2042 Fragments arranged by size: 2042 1393 938 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2342 2588 2955 3632 4780 5025 1606 Size: 478 258 246 367 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 478 367 258 246 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4987 NdeI CA'TA_TG Cuts at: 387 387 Size: 5539 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5539 NotI GC'GGCC_GC Cuts at: 2986 2986 Size: 5539 NspI r_CATG'y Cuts at: 1132 1229 2199 2897 4442 1132 Size: 97 970 698 1545 2229 Fragments arranged by size: 2229 1545 970 698 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5539 PciI A'CATG_T Cuts at: 4438 4438 Size: 5539 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5539 PmeI GTTT'AAAC Cuts at: 2843 2843 Size: 5539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3608 3640 5121 5153 3608 Size: 32 1481 32 3994 Fragments arranged by size: 3994 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5539 PsiI TTA'TAA Cuts at: 3109 3109 Size: 5539 PstI C_TGCA'G Cuts at: 830 2308 2891 830 Size: 1478 583 3478 Fragments arranged by size: 3478 1478 583 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5539 PvuII CAG'CTG Cuts at: 2084 2342 2588 2084 Size: 258 246 5035 Fragments arranged by size: 5035 258 246 SalI G'TCGA_C Cuts at: 1936 2881 1936 Size: 945 4594 SbfI CC_TGCA'GG Cuts at: 2891 2891 Size: 5539 ScaI AGT'ACT Cuts at: 3787 5499 3787 Size: 1712 3827 SfcI C'TryA_G Cuts at: 826 1045 2021 2304 2887 4022 4703 4894 826 Size: 219 976 283 583 1135 681 191 1471 Fragments arranged by size: 1471 1135 976 681 583 283 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5539 SmaI CCC'GGG Cuts at: 1872 2866 1872 Size: 994 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3643 4544 4806 820 Size: 197 926 1700 901 262 1553 Fragments arranged by size: 1700 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3330 493 Size: 2837 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5539 SphI G_CATG'C Cuts at: 1132 2897 1132 Size: 1765 3774 SspI AAT'ATT Cuts at: 5 52 2786 3463 5 Size: 47 2734 677 2081 Fragments arranged by size: 2734 2081 677 47 StuI AGG'CCT Cuts at: 1517 2138 1517 Size: 621 4918 StyI C'CwwG_G Cuts at: 513 1065 2978 513 Size: 552 1913 3074 Fragments arranged by size: 3074 1913 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2080 2143 2714 3566 3583 3736 3921 Size: 1255 500 63 571 852 17 153 185 Cuts at: 3921 4366 325 Size: 445 1498 Fragments arranged by size: 1498 1255 852 571 500 445 185 153 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2180 3785 5497 Size: 274 80 33 51 1443 203 1605 1712 Cuts at: 5497 96 Size: 138 Fragments arranged by size: 1712 1605 1443 274 203 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5499 5531 5499 Size: 32 5507 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5539 XbaI T'CTAG_A Cuts at: 2875 2875 Size: 5539 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5539 XmaI C'CCGG_G Cuts at: 1870 2864 1870 Size: 994 4545 XmnI GAAnn'nnTTC Cuts at: 3668 3668 Size: 5539 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3347 276 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI