(Circular) (Six-base) MAPSORT of: pFN21AB4569.seq Check: 5457 from: 1 to: 6349 pFN21AB4569 6349 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2356 3401 3605 2356 Size: 1045 204 5100 Fragments arranged by size: 5100 1045 204 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3357 3692 1937 Size: 1420 335 4594 Fragments arranged by size: 4594 1420 335 Acc65I G'GTAC_C Cuts at: 3670 3670 Size: 6349 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2079 2483 4412 5796 6064 1632 Size: 447 404 1929 1384 268 1917 Fragments arranged by size: 1929 1917 1384 447 404 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 5248 6225 1203 Size: 4045 977 1327 Fragments arranged by size: 4045 1327 977 AgeI A'CCGG_T Cuts at: 5180 5180 Size: 6349 AhdI GACnn_n'nnGTC Cuts at: 5078 5078 Size: 6349 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6349 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3266 1184 Size: 2082 4267 AlwNI CAG_nnn'CTG Cuts at: 3492 4092 5664 3492 Size: 600 1572 4177 Fragments arranged by size: 4177 1572 600 ApaI G_GGCC'C Cuts at: 3379 3379 Size: 6349 ApaLI G'TGCA_C Cuts at: 4406 5562 4406 Size: 1156 5193 ApoI r'AATT_y Cuts at: 1493 2048 2246 3658 3884 4036 6145 1493 Size: 555 198 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 555 226 198 152 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 3674 1870 Size: 73 1731 4545 Fragments arranged by size: 4545 1731 73 BamHI G'GATC_C Cuts at: 2529 3679 4046 2529 Size: 1150 367 4832 Fragments arranged by size: 4832 1150 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 3389 3670 5125 618 Size: 325 217 627 539 1063 281 1455 1842 Fragments arranged by size: 1842 1455 1063 627 539 325 281 217 BanII G_rGCy'C Cuts at: 1391 2866 3379 3668 1391 Size: 1475 513 289 4072 Fragments arranged by size: 4072 1475 513 289 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2910 3212 928 Size: 971 1011 302 4065 Fragments arranged by size: 4065 1011 971 302 BbvCI CC'TCA_GC Cuts at: 2315 2315 Size: 6349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4540 4574 1903 Size: 34 2603 34 3678 Fragments arranged by size: 3678 2603 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 1548 Size: 6349 BfrBI ATG'CAT Cuts at: 2987 2987 Size: 6349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2553 4960 136 Size: 107 122 71 631 605 881 2407 1525 Fragments arranged by size: 2407 1525 881 631 605 122 107 71 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 3766 3766 Size: 6349 Bme1580I G_kGCm'C Cuts at: 1790 2175 3379 3392 4410 5566 1790 Size: 385 1204 13 1018 1156 2573 Fragments arranged by size: 2573 1204 1156 1018 385 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2572 4080 5038 Size: 416 53 83 399 24 1155 1508 958 Cuts at: 5038 442 Size: 1753 Fragments arranged by size: 1753 1508 1155 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2878 3040 3339 5009 1239 Size: 1639 162 299 1670 2579 Fragments arranged by size: 2579 1670 1639 299 162 Bpu10I CC'TnA_GC Cuts at: 2027 2315 2027 Size: 288 6061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4474 5339 5637 4474 Size: 865 298 5186 Fragments arranged by size: 5186 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3530 5012 882 Size: 617 2031 1482 2219 Fragments arranged by size: 2219 2031 1482 617 BsaAI yAC'GTr Cuts at: 493 1204 2131 4140 493 Size: 711 927 2009 2702 Fragments arranged by size: 2702 2009 927 711 BsaBI GATnn'nnATC Cuts at: 2309 6343 2309 Size: 4034 2315 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4156 4538 5173 275 Size: 53 83 186 936 2623 382 635 1451 Fragments arranged by size: 2623 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4782 5180 5454 5601 6012 4782 Size: 398 274 147 411 5119 Fragments arranged by size: 5119 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6124 Fragments arranged by size: 6124 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2583 2738 2741 3128 1186 Size: 838 559 155 3 387 4407 Fragments arranged by size: 4407 838 559 387 155 3 BseYI C'CCAG_C Cuts at: 2702 2875 5552 6048 2702 Size: 173 2677 496 3003 Fragments arranged by size: 3003 2677 496 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3633 1163 Size: 573 1897 3879 Fragments arranged by size: 3879 1897 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 3799 4560 5588 1540 Size: 275 186 1597 201 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 201 186 BsiHKAI G_wGCw'C Cuts at: 2541 2866 3282 3421 4410 4495 5566 2541 Size: 325 416 139 989 85 1071 3324 Fragments arranged by size: 3324 1071 989 416 325 139 85 BsmI GAATG_Cn' Cuts at: 2987 3609 3860 3953 2987 Size: 622 251 93 5383 Fragments arranged by size: 5383 622 251 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2541 2866 3282 3379 3392 3421 Size: 399 385 366 325 416 97 13 29 Cuts at: 3421 3668 4410 4495 5566 1391 Size: 247 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 416 399 385 366 325 247 97 85 29 13 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 4238 5968 4238 Size: 1730 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2356 3401 3605 3704 844 Size: 1512 1045 204 99 3489 Fragments arranged by size: 3489 1512 1045 204 99 BsrBI CCG'CTC Cuts at: 1394 1823 2746 4236 1394 Size: 429 923 1490 3507 Fragments arranged by size: 3507 1490 923 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2338 2372 3297 4844 5018 65 Size: 1183 1090 34 925 1547 174 1396 Fragments arranged by size: 1547 1396 1183 1090 925 174 34 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 4993 5180 1700 Size: 110 228 2955 187 2869 Fragments arranged by size: 2955 2869 228 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6349 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6349 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstXI CCAn_nnnn'nTGG Cuts at: 2875 2875 Size: 6349 BstYI r'GATC_y Cuts at: 1972 2529 3679 4046 4431 4448 5900 5986 5998 Size: 557 1150 367 385 17 1452 86 12 Cuts at: 5998 6344 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1150 557 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3351 3351 Size: 6349 BtgI C'CryG_G Cuts at: 513 1065 2004 2298 3210 3363 513 Size: 552 939 294 912 153 3499 Fragments arranged by size: 3499 939 912 552 294 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3421 487 Size: 683 56 2195 3415 Fragments arranged by size: 3415 2195 683 56 BtsI GCAGTG_nn' Cuts at: 2229 3865 4659 4679 2229 Size: 1636 794 20 3899 Fragments arranged by size: 3899 1636 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6314 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5356 809 Size: 507 4040 1802 Fragments arranged by size: 4040 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3595 3796 4685 Size: 54 1425 50 275 18 1765 201 889 Cuts at: 4685 6314 8 Size: 1629 43 Fragments arranged by size: 1765 1629 1425 889 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 3796 1537 Size: 275 1783 201 4090 Fragments arranged by size: 4090 1783 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 3032 3299 4279 1353 Size: 1679 267 980 3423 Fragments arranged by size: 3423 1679 980 267 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4936 5310 5456 387 Size: 1064 138 3347 374 146 1280 Fragments arranged by size: 3347 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2864 2864 Size: 6349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2483 2878 3040 3339 4412 5009 Size: 393 447 404 395 162 299 1073 597 Cuts at: 5009 5796 6064 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1073 787 597 447 404 395 393 299 268 162 EcoNI CCTnn'n_nnAGG Cuts at: 2839 3110 2839 Size: 271 6078 EcoO109I rG'GnC_Cy Cuts at: 2545 2923 3376 2545 Size: 378 453 5518 Fragments arranged by size: 5518 453 378 EcoRI G'AATT_C Cuts at: 3658 4036 3658 Size: 378 5971 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 5496 1678 Size: 3818 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2789 2821 4990 5022 5064 Size: 32 135 32 1217 32 2169 32 42 Cuts at: 5064 5096 1373 Size: 32 2626 Fragments arranged by size: 2626 2169 1217 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3693 3939 669 Size: 988 281 1755 246 3079 Fragments arranged by size: 3079 1755 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6349 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KpnI G_GTAC'C Cuts at: 3674 3674 Size: 6349 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3041 3247 5463 5647 1305 Size: 1736 206 2216 184 2007 Fragments arranged by size: 2216 2007 1736 206 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4527 Fragments arranged by size: 4527 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2303 4307 4666 4825 518 Size: 648 1137 2004 359 159 2042 Fragments arranged by size: 2042 2004 1137 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 2168 2335 2738 2875 3399 3765 4442 Size: 429 133 167 403 137 524 366 677 Cuts at: 4442 5590 5835 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 524 429 403 366 245 167 137 133 NcoI C'CATG_G Cuts at: 513 1065 2004 2298 3210 513 Size: 552 939 294 912 3652 Fragments arranged by size: 3652 939 912 552 294 NdeI CA'TA_TG Cuts at: 387 3337 387 Size: 2950 3399 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NsiI A_TGCA'T Cuts at: 2989 2989 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 3707 5252 1132 Size: 97 2478 1545 2229 Fragments arranged by size: 2478 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6349 PciI A'CATG_T Cuts at: 5248 5248 Size: 6349 PflMI CCAn_nnn'nTGG Cuts at: 1108 2421 3589 1108 Size: 1313 1168 3868 Fragments arranged by size: 3868 1313 1168 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PmlI CAC'GTG Cuts at: 2131 2131 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3623 3655 4418 4450 5931 5963 3623 Size: 32 763 32 1481 32 4009 Fragments arranged by size: 4009 1481 763 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2923 2923 Size: 6349 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PspOMI G'GGCC_C Cuts at: 3375 3375 Size: 6349 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2585 2617 2585 Size: 32 6317 PstI C_TGCA'G Cuts at: 830 3245 3398 3558 3701 830 Size: 2415 153 160 143 3478 Fragments arranged by size: 3478 2415 160 153 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 2035 2168 2335 2738 2035 Size: 133 167 403 5646 Fragments arranged by size: 5646 403 167 133 SacI G_AGCT'C Cuts at: 2866 2866 Size: 6349 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SexAI A'CCwGG_T Cuts at: 3623 3623 Size: 6349 SfcI C'TryA_G Cuts at: 826 1045 3072 3241 3394 3554 3697 4832 5513 Size: 219 2027 169 153 160 143 1135 681 Cuts at: 5513 5704 826 Size: 191 1471 Fragments arranged by size: 2027 1471 1135 681 219 191 169 160 153 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 3676 1872 Size: 1804 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4453 5354 5616 820 Size: 197 926 2510 901 262 1553 Fragments arranged by size: 2510 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3707 1132 Size: 2575 3774 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6349 StyI C'CwwG_G Cuts at: 513 1065 2004 2175 2298 3210 3788 513 Size: 552 939 171 123 912 578 3074 Fragments arranged by size: 3074 939 912 578 552 171 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2922 3404 4376 4393 4546 4731 5176 Size: 1255 1342 482 972 17 153 185 445 Cuts at: 5176 325 Size: 1498 Fragments arranged by size: 1498 1342 1255 972 482 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 4595 6307 Size: 274 80 33 51 1443 510 2108 1712 Cuts at: 6307 96 Size: 138 Fragments arranged by size: 2108 1712 1443 510 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3119 3151 3261 3293 6309 6341 3119 Size: 32 110 32 3016 32 3127 Fragments arranged by size: 3127 3016 110 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6349 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 3674 1870 Size: 1804 4545 XmnI GAAnn'nnTTC Cuts at: 4478 4478 Size: 6349 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI