(Circular) (Six-base) MAPSORT of: pFN21AB4374.seq Check: 2483 from: 1 to: 5947 pFN21AB4374 5947 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2786 3290 1937 Size: 849 504 4594 Fragments arranged by size: 4594 849 504 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4010 5394 5662 1632 Size: 2378 1384 268 1917 Fragments arranged by size: 2378 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 4846 5823 1203 Size: 3643 977 1327 Fragments arranged by size: 3643 1327 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5947 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5947 AlwNI CAG_nnn'CTG Cuts at: 3690 5262 3690 Size: 1572 4375 ApaLI G'TGCA_C Cuts at: 4004 5160 4004 Size: 1156 4791 ApoI r'AATT_y Cuts at: 1493 2277 2846 2889 3007 3242 3256 3482 3634 Size: 784 569 43 118 235 14 226 152 Cuts at: 3634 5743 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 784 569 235 226 152 118 43 14 AseI AT'TA_AT Cuts at: 160 1032 2403 2457 4501 160 Size: 872 1371 54 2044 1606 Fragments arranged by size: 2044 1606 1371 872 54 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 3272 1870 Size: 73 1329 4545 Fragments arranged by size: 4545 1329 73 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3268 4723 618 Size: 325 217 627 1481 1455 1842 Fragments arranged by size: 1842 1481 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3266 1391 Size: 1875 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2931 928 Size: 971 1032 3944 Fragments arranged by size: 3944 1032 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2873 2907 4138 4172 1903 Size: 34 936 34 1231 34 3678 Fragments arranged by size: 3678 1231 936 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3839 5049 3839 Size: 1210 4737 BclI T'GATC_A Cuts at: 1548 1548 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4558 136 Size: 107 122 71 631 605 2886 1525 Fragments arranged by size: 2886 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 2880 3364 2880 Size: 484 5463 Bme1580I G_kGCm'C Cuts at: 1790 2268 4008 5164 1790 Size: 478 1740 1156 2573 Fragments arranged by size: 2573 1740 1156 478 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2384 2587 3678 Size: 416 53 83 399 24 967 203 1091 Cuts at: 3678 4636 442 Size: 958 1753 Fragments arranged by size: 1753 1091 967 958 416 399 203 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3068 1056 Size: 2012 3935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4607 1239 Size: 3368 2579 Bpu10I CC'TnA_GC Cuts at: 2726 2726 Size: 5947 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4072 4937 5235 4072 Size: 865 298 4784 Fragments arranged by size: 4784 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2305 4610 882 Size: 617 806 2305 2219 Fragments arranged by size: 2305 2219 806 617 BsaAI yAC'GTr Cuts at: 493 1204 3738 493 Size: 711 2534 2702 Fragments arranged by size: 2702 2534 711 BsaBI GATnn'nnATC Cuts at: 5941 5941 Size: 5947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3754 4136 4771 275 Size: 53 83 186 936 2221 382 635 1451 Fragments arranged by size: 2221 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4380 4778 5052 5199 5610 4380 Size: 398 274 147 411 4717 Fragments arranged by size: 4717 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2048 2078 3113 3143 1178 Size: 30 165 30 645 30 1035 30 3982 Fragments arranged by size: 3982 1035 645 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3085 3242 1186 Size: 1899 157 3891 Fragments arranged by size: 3891 1899 157 BseYI C'CCAG_C Cuts at: 5150 5646 5150 Size: 496 5451 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 2507 1163 Size: 573 483 288 4603 Fragments arranged by size: 4603 573 483 288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3397 4158 5186 1540 Size: 275 186 1396 761 1028 2301 Fragments arranged by size: 2301 1396 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4008 4093 5164 4008 Size: 85 1071 4791 Fragments arranged by size: 4791 1071 85 BsmI GAATG_Cn' Cuts at: 3044 3159 3458 3551 3044 Size: 115 299 93 5440 Fragments arranged by size: 5440 299 115 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 3266 4008 4093 5164 1391 Size: 399 478 998 742 85 1071 2174 Fragments arranged by size: 2174 1071 998 742 478 399 85 BspEI T'CCGG_A Cuts at: 5610 5610 Size: 5947 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3302 844 Size: 2458 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3834 1394 Size: 429 2011 3507 Fragments arranged by size: 3507 2011 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2021 2221 4442 4616 65 Size: 1183 773 200 2221 174 1396 Fragments arranged by size: 2221 1396 1183 773 200 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4591 4778 1700 Size: 110 2781 187 2869 Fragments arranged by size: 2869 2781 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5947 BssSI C'ACGA_G Cuts at: 4007 5019 4007 Size: 1012 4935 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5947 BstBI TT'CG_AA Cuts at: 3048 4751 3048 Size: 1703 4244 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstXI CCAn_nnnn'nTGG Cuts at: 2374 2374 Size: 5947 BstYI r'GATC_y Cuts at: 1972 3175 3277 3644 4029 4046 5498 5584 5596 Size: 1203 102 367 385 17 1452 86 12 Cuts at: 5596 5942 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1203 385 367 346 102 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2564 2564 Size: 5947 BtgI C'CryG_G Cuts at: 513 1065 2052 3171 513 Size: 552 987 1119 3289 Fragments arranged by size: 3289 1119 987 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5208 Fragments arranged by size: 5208 683 56 BtsI GCAGTG_nn' Cuts at: 3463 4257 4277 3463 Size: 794 20 5133 Fragments arranged by size: 5133 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 2466 3251 2466 Size: 785 5162 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2482 3394 4283 Size: 54 1425 50 275 18 652 912 889 Cuts at: 4283 5912 8 Size: 1629 43 Fragments arranged by size: 1629 1425 912 889 652 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3394 1537 Size: 275 1582 4090 Fragments arranged by size: 4090 1582 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2091 3877 1353 Size: 738 1786 3423 Fragments arranged by size: 3423 1786 738 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4534 4908 5054 387 Size: 1064 138 2945 374 146 1280 Fragments arranged by size: 2945 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4010 4607 5394 5662 1239 Size: 393 2378 597 787 268 1524 Fragments arranged by size: 2378 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2097 3038 2097 Size: 941 5006 EcoO109I rG'GnC_Cy Cuts at: 2102 2806 2917 2102 Size: 704 111 5132 Fragments arranged by size: 5132 704 111 EcoRI G'AATT_C Cuts at: 2277 3256 3634 2277 Size: 979 378 4590 Fragments arranged by size: 4590 979 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2686 2686 Size: 5947 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 5094 1678 Size: 3416 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2100 2132 2948 Size: 32 135 32 476 32 20 32 816 Cuts at: 2948 2980 4588 4620 4662 4694 1373 Size: 32 1608 32 42 32 2626 Fragments arranged by size: 2626 1608 816 476 135 42 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 3291 3537 669 Size: 988 281 1353 246 3079 Fragments arranged by size: 3079 1353 988 281 246 HindIII A'AGCT_T Cuts at: 748 2663 2691 3112 748 Size: 1915 28 421 3583 Fragments arranged by size: 3583 1915 421 28 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 3546 3546 Size: 5947 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 2220 5061 5245 1305 Size: 813 102 2841 184 2007 Fragments arranged by size: 2841 2007 813 184 102 MscI TGG'CCA Cuts at: 10 64 1489 1832 2484 10 Size: 54 1425 343 652 3473 Fragments arranged by size: 3473 1425 652 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 3905 4264 4423 518 Size: 648 1251 1488 359 159 2042 Fragments arranged by size: 2042 1488 1251 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3363 4040 5188 5433 1606 Size: 1757 677 1148 245 2120 Fragments arranged by size: 2120 1757 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5395 NdeI CA'TA_TG Cuts at: 387 387 Size: 5947 NheI G'CTAG_C Cuts at: 1052 3064 1052 Size: 2012 3935 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 3305 4850 1132 Size: 97 2076 1545 2229 Fragments arranged by size: 2229 2076 1545 97 PasI CC'CwG_GG Cuts at: 1348 3034 1348 Size: 1686 4261 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2125 2176 1108 Size: 902 115 51 4879 Fragments arranged by size: 4879 902 115 51 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2102 2806 2917 2102 Size: 704 111 5132 Fragments arranged by size: 5132 704 111 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5947 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PstI C_TGCA'G Cuts at: 830 3299 830 Size: 2469 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 2642 4195 5907 2642 Size: 1553 1712 2682 Fragments arranged by size: 2682 1712 1553 SfcI C'TryA_G Cuts at: 826 1045 2282 2346 2787 3295 4430 5111 5302 Size: 219 1237 64 441 508 1135 681 191 Cuts at: 5302 826 Size: 1471 Fragments arranged by size: 1471 1237 1135 681 508 441 219 191 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 3274 1872 Size: 1402 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4051 4952 5214 820 Size: 197 926 2108 901 262 1553 Fragments arranged by size: 2108 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 3305 1132 Size: 2173 3774 SspI AAT'ATT Cuts at: 5 52 3871 5 Size: 47 3819 2081 Fragments arranged by size: 3819 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5947 StyI C'CwwG_G Cuts at: 513 1065 3386 513 Size: 552 2321 3074 Fragments arranged by size: 3074 2321 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2541 2670 3974 3991 4144 4329 4774 Size: 1255 961 129 1304 17 153 185 445 Cuts at: 4774 325 Size: 1498 Fragments arranged by size: 1498 1304 1255 961 445 185 153 129 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2640 4193 5905 Size: 274 80 33 51 1443 663 1553 1712 Cuts at: 5905 96 Size: 138 Fragments arranged by size: 1712 1553 1443 663 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5907 5939 5907 Size: 32 5915 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 3272 1870 Size: 1402 4545 XmnI GAAnn'nnTTC Cuts at: 4076 4076 Size: 5947 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI