(Circular) (Six-base) MAPSORT of: pFN21AB4278.seq Check: 7370 from: 1 to: 5737 pFN21AB4278 5737 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3547 278 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2073 3080 1937 Size: 136 1007 4594 Fragments arranged by size: 4594 1007 136 Acc65I G'GTAC_C Cuts at: 3058 3058 Size: 5737 AclI AA'CG_TT Cuts at: 1563 3864 4237 1563 Size: 2301 373 3063 Fragments arranged by size: 3063 2301 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2508 3800 5184 5452 1632 Size: 876 1292 1384 268 1917 Fragments arranged by size: 1917 1384 1292 876 268 AflII C'TTAA_G Cuts at: 820 1017 2798 820 Size: 197 1781 3759 Fragments arranged by size: 3759 1781 197 AflIII A'CryG_T Cuts at: 1203 4636 5613 1203 Size: 3433 977 1327 Fragments arranged by size: 3433 1327 977 AgeI A'CCGG_T Cuts at: 4568 4568 Size: 5737 AhdI GACnn_n'nnGTC Cuts at: 4466 4466 Size: 5737 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5737 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5737 AlwNI CAG_nnn'CTG Cuts at: 3480 5052 3480 Size: 1572 4165 ApaLI G'TGCA_C Cuts at: 2986 3794 4950 2986 Size: 808 1156 3773 Fragments arranged by size: 3773 1156 808 ApoI r'AATT_y Cuts at: 1493 2679 3046 3272 3424 5533 1493 Size: 1186 367 226 152 2109 1697 Fragments arranged by size: 2109 1697 1186 367 226 152 AseI AT'TA_AT Cuts at: 160 1032 2712 4291 160 Size: 872 1680 1579 1606 Fragments arranged by size: 1680 1606 1579 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2103 3062 1870 Size: 73 114 46 959 4545 Fragments arranged by size: 4545 959 114 73 46 BamHI G'GATC_C Cuts at: 3067 3434 3067 Size: 367 5370 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3058 4513 618 Size: 325 217 627 1271 1455 1842 Fragments arranged by size: 1842 1455 1271 627 325 217 BanII G_rGCy'C Cuts at: 1391 3056 1391 Size: 1665 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2355 928 Size: 971 456 4310 Fragments arranged by size: 4310 971 456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3928 3962 1903 Size: 34 1991 34 3678 Fragments arranged by size: 3678 1991 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3629 4839 3629 Size: 1210 4527 BclI T'GATC_A Cuts at: 1548 2977 1548 Size: 1429 4308 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4348 136 Size: 107 122 71 631 605 2676 1525 Fragments arranged by size: 2676 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2794 5732 2794 Size: 2938 2799 BlpI GC'TnA_GC Cuts at: 2565 3154 2565 Size: 589 5148 Bme1580I G_kGCm'C Cuts at: 1790 2990 3798 4954 1790 Size: 1200 808 1156 2573 Fragments arranged by size: 2573 1200 1156 808 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5737 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2967 3468 4426 Size: 416 53 83 399 24 1550 501 958 Cuts at: 4426 442 Size: 1753 Fragments arranged by size: 1753 1550 958 501 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2755 4397 1239 Size: 1516 1642 2579 Fragments arranged by size: 2579 1642 1516 Bpu10I CC'TnA_GC Cuts at: 2868 2868 Size: 5737 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3862 4727 5025 3862 Size: 865 298 4574 Fragments arranged by size: 4574 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4400 882 Size: 617 2901 2219 Fragments arranged by size: 2901 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3528 493 Size: 711 2324 2702 Fragments arranged by size: 2702 2324 711 BsaBI GATnn'nnATC Cuts at: 5731 5731 Size: 5737 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3544 3926 4561 275 Size: 53 83 186 936 2011 382 635 1451 Fragments arranged by size: 2011 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2382 4170 4568 4842 4989 5400 2382 Size: 1788 398 274 147 411 2719 Fragments arranged by size: 2719 1788 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5512 Fragments arranged by size: 5512 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2958 2963 1186 Size: 1772 5 3960 Fragments arranged by size: 3960 1772 5 BseYI C'CCAG_C Cuts at: 4940 5436 4940 Size: 496 5241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3187 3948 4976 1540 Size: 275 186 1186 761 1028 2301 Fragments arranged by size: 2301 1186 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 2990 3798 3883 4954 2028 Size: 962 808 85 1071 2811 Fragments arranged by size: 2811 1071 962 808 85 BsmI GAATG_Cn' Cuts at: 3248 3341 3248 Size: 93 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2990 3056 3798 3883 4954 1391 Size: 399 238 962 66 742 85 1071 2174 Fragments arranged by size: 2174 1071 962 742 399 238 85 66 BspEI T'CCGG_A Cuts at: 2382 5400 2382 Size: 3018 2719 BspHI T'CATG_A Cuts at: 2038 2371 3626 5356 2038 Size: 333 1255 1730 2419 Fragments arranged by size: 2419 1730 1255 333 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2024 3092 844 Size: 1180 1068 3489 Fragments arranged by size: 3489 1180 1068 BsrBI CCG'CTC Cuts at: 1394 1823 3624 1394 Size: 429 1801 3507 Fragments arranged by size: 3507 1801 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4232 4406 65 Size: 1183 2984 174 1396 Fragments arranged by size: 2984 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4381 4568 1700 Size: 110 2571 187 2869 Fragments arranged by size: 2869 2571 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5737 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5737 BssSI C'ACGA_G Cuts at: 3797 4809 3797 Size: 1012 4725 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5737 BstBI TT'CG_AA Cuts at: 4541 4541 Size: 5737 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5737 BstYI r'GATC_y Cuts at: 1972 2216 2794 3067 3434 3819 3836 5288 5374 Size: 244 578 273 367 385 17 1452 86 Cuts at: 5374 5386 5732 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 578 385 367 346 273 244 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2886 2886 Size: 5737 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5185 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4998 Fragments arranged by size: 4998 683 56 BtsI GCAGTG_nn' Cuts at: 3253 4047 4067 3253 Size: 794 20 4923 Fragments arranged by size: 4923 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5737 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5702 DraI TTT'AAA Cuts at: 3041 3041 Size: 5737 DraIII CAC_nnn'GTG Cuts at: 2157 2157 Size: 5737 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4744 809 Size: 507 3428 1802 Fragments arranged by size: 3428 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3184 4073 5702 Size: 54 1425 50 275 18 1354 889 1629 Cuts at: 5702 8 Size: 43 Fragments arranged by size: 1629 1425 1354 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3184 1537 Size: 275 1372 4090 Fragments arranged by size: 4090 1372 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 3667 1353 Size: 729 1585 3423 Fragments arranged by size: 3423 1585 729 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4324 4698 4844 387 Size: 1064 138 2735 374 146 1280 Fragments arranged by size: 2735 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2508 2755 3800 4397 5184 5452 1239 Size: 393 876 247 1045 597 787 268 1524 Fragments arranged by size: 1524 1045 876 787 597 393 268 247 EcoRI G'AATT_C Cuts at: 3046 3424 3046 Size: 378 5359 FspI TGC'GCA Cuts at: 4243 4243 Size: 5737 HaeII r_GCGC'y Cuts at: 1678 4884 1678 Size: 3206 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4378 4410 4452 4484 1373 Size: 32 135 32 2806 32 42 32 2626 Fragments arranged by size: 2806 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3327 669 Size: 988 281 1143 246 3079 Fragments arranged by size: 3079 1143 988 281 246 HindIII A'AGCT_T Cuts at: 748 2574 748 Size: 1826 3911 HpaI GTT'AAC Cuts at: 3327 3327 Size: 5737 KpnI G_GTAC'C Cuts at: 3062 3062 Size: 5737 MfeI C'AATT_G Cuts at: 3336 3336 Size: 5737 MluI A'CGCG_T Cuts at: 5613 5613 Size: 5737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2074 2122 4851 5035 1305 Size: 769 48 2729 184 2007 Fragments arranged by size: 2729 2007 769 184 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3915 Fragments arranged by size: 3915 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3695 4054 4213 518 Size: 648 2529 359 159 2042 Fragments arranged by size: 2529 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2569 3153 3830 4978 5223 1606 Size: 963 584 677 1148 245 2120 Fragments arranged by size: 2120 1148 963 677 584 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5185 NdeI CA'TA_TG Cuts at: 387 387 Size: 5737 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5737 NotI GC'GGCC_GC Cuts at: 3184 3184 Size: 5737 NspI r_CATG'y Cuts at: 1132 1229 3095 4640 1132 Size: 97 1866 1545 2229 Fragments arranged by size: 2229 1866 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5737 PciI A'CATG_T Cuts at: 4636 4636 Size: 5737 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5737 PfoI T'CCnGG_A Cuts at: 2161 2161 Size: 5737 PmeI GTTT'AAAC Cuts at: 3041 3041 Size: 5737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3806 3838 5319 5351 3806 Size: 32 1481 32 4192 Fragments arranged by size: 4192 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5737 PsiI TTA'TAA Cuts at: 3307 3307 Size: 5737 PstI C_TGCA'G Cuts at: 830 3089 830 Size: 2259 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5737 PvuII CAG'CTG Cuts at: 2569 2569 Size: 5737 SalI G'TCGA_C Cuts at: 1936 3079 1936 Size: 1143 4594 SbfI CC_TGCA'GG Cuts at: 3089 3089 Size: 5737 ScaI AGT'ACT Cuts at: 3985 5697 3985 Size: 1712 4025 SfcI C'TryA_G Cuts at: 826 1045 2857 3085 4220 4901 5092 826 Size: 219 1812 228 1135 681 191 1471 Fragments arranged by size: 1812 1471 1135 681 228 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5737 SmaI CCC'GGG Cuts at: 1872 3064 1872 Size: 1192 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2103 2798 3841 4742 5004 820 Size: 197 926 160 695 1043 901 262 1553 Fragments arranged by size: 1553 1043 926 901 695 262 197 160 SnaBI TAC'GTA Cuts at: 493 3528 493 Size: 3035 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5737 SphI G_CATG'C Cuts at: 1132 3095 1132 Size: 1963 3774 SspI AAT'ATT Cuts at: 5 52 2251 2359 3661 5 Size: 47 2199 108 1302 2081 Fragments arranged by size: 2199 2081 1302 108 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5737 StyI C'CwwG_G Cuts at: 513 1065 3176 513 Size: 552 2111 3074 Fragments arranged by size: 3074 2111 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3764 3781 3934 4119 4564 325 Size: 1255 2184 17 153 185 445 1498 Fragments arranged by size: 2184 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2402 3983 5695 Size: 274 80 33 51 1443 425 1581 1712 Cuts at: 5695 96 Size: 138 Fragments arranged by size: 1712 1581 1443 425 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2143 2175 5697 5729 2143 Size: 32 3522 32 2151 Fragments arranged by size: 3522 2151 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5737 XbaI T'CTAG_A Cuts at: 3073 3073 Size: 5737 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5737 XhoI C'TCGA_G Cuts at: 1943 2103 1943 Size: 160 5577 XmaI C'CCGG_G Cuts at: 1870 3062 1870 Size: 1192 4545 XmnI GAAnn'nnTTC Cuts at: 3866 3866 Size: 5737 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3545 276 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI