Restriction map for
pFN21AB4223
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BanII BbrPI BbsI BcgI
BciVI BfaI BfrI BglI BlnI
BmyI BpuAI BsaAI BsaBI BsaI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoT22I FseI FspI HindIII
HinfI HpaI KpnI KspI MaeI
MamI MfeI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1039 GACGTC GACGT^C
AccIII 969 TCCGGA T^CCGGA
AlwI 123 GATCC GGATC(4/5)
AspI 450 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1094 TGGCCA TGG^CCA
BanI 39 GGCGCC G^G[CT][GA]CC
BbeI 39 GGCGCC GGCGC^C
BclI 339 TGATCA T^GATCA
BglII 1181 AGATCT A^GATCT
BpmI 781 CTGGAG CTGGAG(16/14)
BsaWI 969 TCCGGA [AT]^CCGG[AT]
BseAI 969 TCCGGA T^CCGGA
BsiEI 429 CGACCG CG[GA][CT]^CG
BsmFI 797 GGGAC GGGAC(10/14)
BspEI 969 TCCGGA T^CCGGA
BspMI 263 ACCTGC ACCTGC(4/8)
BsrDI 173 GCAATG GCAATG(2/0)
BsrFI 431 ACCGGC [GA]^CCGG[CT]
BsrI 504 CCAGT ACTGG(1/-1)
BstEII 597 GGTTACC G^GT[ACGT]ACC
BstPI 597 GGTTACC G^GT[ACGT]ACC
BstXI 23 CCACGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1181 AGATCT [GA]^GATC[CT]
Cfr10I 431 ACCGGC [GA]^CCGG[CT]
DraIII 856 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1094 TGGCCA [CT]^GGCC[GA]
EarI 247 CTCTTC CTCTTC(1/4)
EcoO65I 597 GGTTACC G^GT[ACGT]ACC
EcoRV 1105 GATATC GAT^ATC
FbaI 339 TGATCA T^GATCA
HincII 1036 GTTGAC GT[CT]^[GA]AC
HindII 1036 GTTGAC GT[CT]^[GA]AC
KasI 39 GGCGCC G^GCGCC
Ksp632I 247 CTCTTC CTCTTC(1/4)
MflI 1181 AGATCT [GA]^GATC[CT]
MluI 409 ACGCGT A^CGCGT
MluNI 1094 TGGCCA TGG^CCA
MroI 969 TCCGGA T^CCGGA
MscI 1094 TGGCCA TGG^CCA
MspA1I 817 CAGCTG C[AC]G^C[GT]G
NarI 39 GGCGCC GG^CGCC
NcoI 299 CCATGG C^CATGG
PflFI 450 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 855 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 817 CAGCTG CAG^CTG
ScaI 1205 AGTACT AGT^ACT
SfoI 39 GGCGCC GGC^GCC
SmlI 649 CTCAAG C^T[CT][GA]AG
Tth111I 450 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 855 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1181 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 365 CGCG CG^CG
410 CGCG
AcsI 354 GAATTC [GA]^AATT[CT]
1021 GAATTC
AcyI 39 GGCGCC G[GA]^CG[CT]C
1039 GACGTC
AfaI 558 GTAC GT^AC
1206 GTAC
AflIII 409 ACGCGT A^C[GA][CT]GT
608 ACGTGT
AlwNI 544 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
742 CAGAAGCTG
ApoI 354 GAATTC [GA]^AATT[CT]
1021 GAATTC
AvaII 864 GGACC G^G[AT]CC
1012 GGTCC
BbvCI 126 CCTCAGC CCTCAGC(-5/-2)
990 CCTCAGC
BcnI 108 CCGGG CC^[GC]GG
867 CCGGG
BlpI 45 GCTCAGC GC^T[ACGT]AGC
168 GCTGAGC
Bpu1102I 45 GCTCAGC GC^T[ACGT]AGC
168 GCTGAGC
BsaHI 39 GGCGCC G[GA]^CG[CT]C
1039 GACGTC
BsgI 12 CTGCAC GTGCAG(16/14)
1139 CTGCAC
BsrBI 43 CCGCTC CCGCTC(-3/-3)
898 GAGCGG
BstAPI 741 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1141 GCACATGCTGC
BstUI 365 CGCG CG^CG
410 CGCG
CelII 45 GCTCAGC GC^T[ACGT]AGC
168 GCTGAGC
EcoRI 354 GAATTC G^AATTC
1021 GAATTC
EcoT14I 299 CCATGG C^C[AT][AT]GG
695 CCAAGG
FauI 795 GCGGG CCCGC(4/6)
900 GCGGG
HgaI 363 GACGC GACGC(5/10)
131 GCGTC
Hin1I 39 GGCGCC G[GA]^CG[CT]C
1039 GACGTC
MslI 24 CACGGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
192 CATGCTAATG
MvnI 365 CGCG CG^CG
410 CGCG
NciI 108 CCGGG CC^[GC]GG
867 CCGGG
NlaIV 39 GGCGCC GG[ACGT]^[ACGT]CC
1174 GGGGCC
RsaI 558 GTAC GT^AC
1206 GTAC
SfcI 219 CTACAG C^T[GA][CT]AG
834 CTACAG
StyI 299 CCATGG C^C[AT][AT]GG
695 CCAAGG
TaqI 1025 TCGA T^CGA
1186 TCGA
Tsp45I 361 GTGAC ^GT[GC]AC
386 GTGAC
TthHB8I 1025 TCGA T^CGA
1186 TCGA
XcmI 393 CCAGGGTCGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
458 CCAGCGAGGACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI Asp700I BbvI BsaJI
BseRI BsiYI BslI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NspI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp509I TspRI XmnI