(Circular) (Six-base) MAPSORT of: pFN21AB4190.seq Check: 9476 from: 1 to: 5797 pFN21AB4190 5797 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2531 2531 Size: 5797 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 2744 3860 5244 5512 1632 Size: 410 702 1116 1384 268 1917 Fragments arranged by size: 1917 1384 1116 702 410 268 AfeI AGC'GCT Cuts at: 2281 2281 Size: 5797 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 4696 5673 1203 Size: 3493 977 1327 Fragments arranged by size: 3493 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 2657 1166 Size: 1491 4306 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2372 1184 Size: 1188 4609 AlwNI CAG_nnn'CTG Cuts at: 2248 2685 2767 3030 3540 5112 2248 Size: 437 82 263 510 1572 2933 Fragments arranged by size: 2933 1572 510 437 263 82 ApaI G_GGCC'C Cuts at: 2017 2017 Size: 5797 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 3106 3332 3484 5593 1493 Size: 1613 226 152 2109 1697 Fragments arranged by size: 2109 1697 1613 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 2750 3122 1870 Size: 73 807 372 4545 Fragments arranged by size: 4545 807 372 73 BamHI G'GATC_C Cuts at: 2034 3127 3494 2034 Size: 1093 367 4337 Fragments arranged by size: 4337 1093 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2579 2597 2688 3118 Size: 325 217 627 516 276 18 91 430 Cuts at: 3118 4573 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 516 430 325 276 217 91 18 BanII G_rGCy'C Cuts at: 1391 2017 3060 3116 1391 Size: 626 1043 56 4072 Fragments arranged by size: 4072 1043 626 56 BbeI G_GCGC'C Cuts at: 2692 2692 Size: 5797 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2365 2452 2596 3050 928 Size: 971 466 87 144 454 3675 Fragments arranged by size: 3675 971 466 454 144 87 BbvCI CC'TCA_GC Cuts at: 2276 2276 Size: 5797 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2587 2621 3988 4022 1903 Size: 34 650 34 1367 34 3678 Fragments arranged by size: 3678 1367 650 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 2385 1548 Size: 837 4960 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2832 4408 136 Size: 107 122 71 631 605 1160 1576 1525 Fragments arranged by size: 1576 1525 1160 631 605 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 2017 2600 2797 2906 2923 3858 5014 1790 Size: 227 583 197 109 17 935 1156 2573 Fragments arranged by size: 2573 1156 935 583 227 197 109 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2158 3528 4486 Size: 416 53 83 399 24 741 1370 958 Cuts at: 4486 442 Size: 1753 Fragments arranged by size: 1753 1370 958 741 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2622 1056 Size: 1566 4231 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4457 1239 Size: 3218 2579 Bpu10I CC'TnA_GC Cuts at: 2276 2276 Size: 5797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2393 2474 3922 4787 5085 2393 Size: 81 1448 865 298 3105 Fragments arranged by size: 3105 1448 865 298 81 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2143 2948 4460 882 Size: 617 644 805 1512 2219 Fragments arranged by size: 2219 1512 805 644 617 BsaAI yAC'GTr Cuts at: 493 1204 2851 3588 493 Size: 711 1647 737 2702 Fragments arranged by size: 2702 1647 737 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2689 3604 3986 4621 Size: 53 83 186 936 1156 915 382 635 Cuts at: 4621 275 Size: 1451 Fragments arranged by size: 1451 1156 936 915 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3049 4230 4628 4902 5049 5460 3049 Size: 1181 398 274 147 411 3386 Fragments arranged by size: 3386 1181 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2186 2216 1178 Size: 30 165 30 783 30 4759 Fragments arranged by size: 4759 783 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2966 2995 3068 1186 Size: 1780 29 73 3915 Fragments arranged by size: 3915 1780 73 29 BseYI C'CCAG_C Cuts at: 2318 2593 2781 2785 2818 2995 3030 3059 5000 Size: 275 188 4 33 177 35 29 1941 Cuts at: 5000 5496 2318 Size: 496 2619 Fragments arranged by size: 2619 1941 496 275 188 177 35 33 29 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2024 1163 Size: 573 288 4936 Fragments arranged by size: 4936 573 288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2664 2943 3858 3943 5014 2664 Size: 279 915 85 1071 3447 Fragments arranged by size: 3447 1071 915 279 85 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2600 2664 2797 2906 2923 2943 Size: 399 227 583 64 133 109 17 20 Cuts at: 2943 3060 3116 3858 3943 5014 1391 Size: 117 56 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 583 399 227 133 117 109 85 64 56 20 17 BspEI T'CCGG_A Cuts at: 3049 5460 3049 Size: 2411 3386 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2196 2490 2531 3152 844 Size: 1352 294 41 621 3489 Fragments arranged by size: 3489 1352 621 294 41 BsrBI CCG'CTC Cuts at: 1394 1823 2445 3684 1394 Size: 429 622 1239 3507 Fragments arranged by size: 3507 1239 622 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2117 4292 4466 65 Size: 1183 869 2175 174 1396 Fragments arranged by size: 2175 1396 1183 869 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4441 4628 1700 Size: 110 2631 187 2869 Fragments arranged by size: 2869 2631 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 2152 2251 1184 Size: 968 99 4730 Fragments arranged by size: 4730 968 99 BstXI CCAn_nnnn'nTGG Cuts at: 2167 3094 2167 Size: 927 4870 BstYI r'GATC_y Cuts at: 1972 2034 3127 3494 3879 3896 5348 5434 5446 Size: 62 1093 367 385 17 1452 86 12 Cuts at: 5446 5792 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1093 385 367 346 86 62 17 12 Bsu36I CC'TnA_GG Cuts at: 2336 2336 Size: 5797 BtgI C'CryG_G Cuts at: 513 1065 2166 2307 3087 513 Size: 552 1101 141 780 3223 Fragments arranged by size: 3223 1101 780 552 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 2797 3313 4107 4127 2797 Size: 516 794 20 4467 Fragments arranged by size: 4467 794 516 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DraIII CAC_nnn'GTG Cuts at: 2896 2896 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 2907 2934 Size: 54 1425 50 275 18 456 621 27 Cuts at: 2934 3244 4133 5762 8 Size: 310 889 1629 43 Fragments arranged by size: 1629 1425 889 621 456 310 275 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2732 3074 3727 1353 Size: 1379 342 653 3423 Fragments arranged by size: 3423 1379 653 342 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2998 4384 4758 4904 387 Size: 1064 138 1409 1386 374 146 1280 Fragments arranged by size: 1409 1386 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2744 3860 4457 5244 5512 1239 Size: 393 410 702 1116 597 787 268 1524 Fragments arranged by size: 1524 1116 787 702 597 410 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3022 3022 Size: 5797 EcoO109I rG'GnC_Cy Cuts at: 2013 2014 2058 2764 2013 Size: 1 44 706 5046 Fragments arranged by size: 5046 706 44 1 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2051 2283 2692 3087 4944 1678 Size: 373 232 409 395 1857 2531 Fragments arranged by size: 2531 1857 409 395 373 232 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2850 2882 4438 Size: 32 135 32 614 32 632 32 1556 Cuts at: 4438 4470 4512 4544 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1556 632 614 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2713 3141 3387 669 Size: 988 281 775 428 246 3079 Fragments arranged by size: 3079 988 775 428 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KasI G'GCGC_C Cuts at: 2688 2688 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4911 5095 1305 Size: 3606 184 2007 Fragments arranged by size: 3606 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3975 Fragments arranged by size: 3975 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2381 2657 2990 3092 3755 4114 Size: 648 999 216 276 333 102 663 359 Cuts at: 4114 4273 518 Size: 159 2042 Fragments arranged by size: 2042 999 663 648 359 333 276 216 159 102 MspA1I CmG'CkG Cuts at: 1606 2785 2884 3213 3890 5038 5283 1606 Size: 1179 99 329 677 1148 245 2120 Fragments arranged by size: 2120 1179 1148 677 329 245 99 NarI GG'CG_CC Cuts at: 2689 2689 Size: 5797 NcoI C'CATG_G Cuts at: 513 1065 2166 3087 513 Size: 552 1101 921 3223 Fragments arranged by size: 3223 1101 921 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 2618 1052 Size: 1566 4231 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 3155 4700 1132 Size: 97 1926 1545 2229 Fragments arranged by size: 2229 1926 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 1108 Size: 1140 4657 PfoI T'CCnGG_A Cuts at: 2030 2030 Size: 5797 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2013 2013 Size: 5797 PstI C_TGCA'G Cuts at: 830 2077 2865 3149 830 Size: 1247 788 284 3478 Fragments arranged by size: 3478 1247 788 284 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2785 2785 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SapI GCTCTTCn'nnn_ Cuts at: 3074 3074 Size: 5797 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 2073 2861 3145 4280 4961 5152 826 Size: 219 1028 788 284 1135 681 191 1471 Fragments arranged by size: 1471 1135 1028 788 681 284 219 191 SfoI GGC'GCC Cuts at: 2690 2690 Size: 5797 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 2489 3901 4802 5064 820 Size: 197 926 465 81 1412 901 262 1553 Fragments arranged by size: 1553 1412 926 901 465 262 197 81 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 2274 2915 1517 Size: 757 641 4399 Fragments arranged by size: 4399 757 641 StyI C'CwwG_G Cuts at: 513 1065 2166 2910 3087 3236 513 Size: 552 1101 744 177 149 3074 Fragments arranged by size: 3074 1101 744 552 177 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3824 3841 3994 4179 4624 325 Size: 1255 2244 17 153 185 445 1498 Fragments arranged by size: 2244 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5755 96 Size: 274 80 33 51 1443 2066 1712 138 Fragments arranged by size: 2066 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2326 2326 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI