Restriction map for
pFN21AB4120
(Linear) (Six-base) MAPSORT of: KIBB4120.seq Check: 156 from: 1 to: 1353
KIBB4120.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 387 1353
Size: 387 966
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 510 542 1353
Size: 510 32 811
Fragments arranged by size:
811 510 32
AlwNI CAG_nnn'CTG
Cuts at: 0 75 225 835 1353
Size: 75 150 610 518
Fragments arranged by size:
610 518 150 75
ApaI G_GGCC'C
Cuts at: 0 1158 1353
Size: 1158 195
ApoI r'AATT_y
Cuts at: 0 987 1353
Size: 987 366
AvaI C'yCGr_G
Cuts at: 0 80 822 1353
Size: 80 742 531
Fragments arranged by size:
742 531 80
BamHI G'GATC_C
Cuts at: 0 1143 1353
Size: 1143 210
BanII G_rGCy'C
Cuts at: 0 829 1158 1353
Size: 829 329 195
Fragments arranged by size:
829 329 195
BbsI GAAGACnn'nnnn_
Cuts at: 0 254 1353
Size: 254 1099
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 853 887 1353
Size: 853 34 466
Fragments arranged by size:
853 466 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 904 1353
Size: 904 449
BfrBI ATG'CAT
Cuts at: 0 199 1353
Size: 199 1154
BlpI GC'TnA_GC
Cuts at: 0 828 950 1353
Size: 828 122 403
Fragments arranged by size:
828 403 122
Bme1580I G_kGCm'C
Cuts at: 0 1158 1353
Size: 1158 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 583 837 1353
Size: 583 254 516
Fragments arranged by size:
583 516 254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1353
Size: 202 1151
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 696 1353
Size: 139 557 657
Fragments arranged by size:
657 557 139
BsaBI GATnn'nnATC
Cuts at: 0 1142 1353
Size: 1142 211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 510 540 1353
Size: 22 30 458 30 813
Fragments arranged by size:
813 458 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 43 157 1114 1353
Size: 40 3 114 957 239
Fragments arranged by size:
957 239 114 40 3
BsmI GAATG_Cn'
Cuts at: 0 773 1353
Size: 773 580
Bsp1286I G_dGCh'C
Cuts at: 0 829 1158 1353
Size: 829 329 195
Fragments arranged by size:
829 329 195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 387 1353
Size: 387 966
BsrDI GCAATG_nn'
Cuts at: 0 779 1353
Size: 779 574
BstYI r'GATC_y
Cuts at: 0 32 1143 1353
Size: 32 1111 210
Fragments arranged by size:
1111 210 32
BtgI C'CryG_G
Cuts at: 0 450 1353
Size: 450 903
ClaI AT'CG_AT
Cuts at: 0 422 1353
Size: 422 931
DraI TTT'AAA
Cuts at: 0 695 1353
Size: 695 658
DraIII CAC_nnn'GTG
Cuts at: 0 718 1353
Size: 718 635
EaeI y'GGCC_r
Cuts at: 0 203 1353
Size: 203 1150
EarI CTCTTCn'nnn_
Cuts at: 0 312 1353
Size: 312 1041
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1353
Size: 202 1151
EcoO109I rG'GnC_Cy
Cuts at: 0 1155 1353
Size: 1155 198
EcoRV GAT'ATC
Cuts at: 0 231 869 1353
Size: 231 638 484
Fragments arranged by size:
638 484 231
HaeII r_GCGC'y
Cuts at: 0 55 396 1353
Size: 55 341 957
Fragments arranged by size:
957 341 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 507 539 1207 1239 1353
Size: 22 32 453 32 668 32 114
Fragments arranged by size:
668 453 114 32 32 32 22
HindIII A'AGCT_T
Cuts at: 0 134 1353
Size: 134 1219
MfeI C'AATT_G
Cuts at: 0 992 1353
Size: 992 361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 1353
Size: 1030 323
MscI TGG'CCA
Cuts at: 0 205 1353
Size: 205 1148
MslI CAynn'nnrTG
Cuts at: 0 784 962 1042 1353
Size: 784 178 80 311
Fragments arranged by size:
784 311 178 80
NcoI C'CATG_G
Cuts at: 0 450 1353
Size: 450 903
NdeI CA'TA_TG
Cuts at: 0 1131 1353
Size: 1131 222
NsiI A_TGCA'T
Cuts at: 0 201 1353
Size: 201 1152
PasI CC'CwG_GG
Cuts at: 0 1315 1353
Size: 1315 38
PflMI CCAn_nnn'nTGG
Cuts at: 0 225 1353
Size: 225 1128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 510 542 1353
Size: 510 32 811
Fragments arranged by size:
811 510 32
PsiI TTA'TAA
Cuts at: 0 593 1255 1353
Size: 593 662 98
Fragments arranged by size:
662 593 98
PspOMI G'GGCC_C
Cuts at: 0 1154 1353
Size: 1154 199
PstI C_TGCA'G
Cuts at: 0 14 1110 1353
Size: 14 1096 243
Fragments arranged by size:
1096 243 14
SfcI C'TryA_G
Cuts at: 0 10 119 1106 1353
Size: 10 109 987 247
Fragments arranged by size:
987 247 109 10
SmaI CCC'GGG
Cuts at: 0 824 1353
Size: 824 529
StyI C'CwwG_G
Cuts at: 0 450 706 1353
Size: 450 256 647
Fragments arranged by size:
647 450 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 819 1353
Size: 819 534
TatI w'GTAC_w
Cuts at: 0 213 1353
Size: 213 1140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 702 734 1353
Size: 702 32 619
Fragments arranged by size:
702 619 32
XmaI C'CCGG_G
Cuts at: 0 822 1353
Size: 822 531
Enzymes that do cut:
AarI AloI AlwNI ApaI ApoI AvaI BamHI BanII
BbsI BcgI BciVI BfrBI BlpI Bme1580I BmrI BpmI
BsaI BsaBI BsaXI BseRI BsmI Bsp1286I BspMI BsrDI
BstYI BtgI ClaI DraI DraIII EaeI EarI Eco57MI
EcoO109I EcoRV HaeII Hin4I HindIII MfeI MmeI MscI
MslI NcoI NdeI NsiI PasI PflMI PpiI PsiI
PspOMI PstI SfcI SmaI StyI TaqII TatI TstI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BclI BglI BglII
BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI CspCI DrdI EagI
EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HincII HpaI KasI KpnI MluI MspA1I NaeI NarI
NgoMIV NheI NotI NruI NspI PacI PciI PfoI
PmeI PmlI PpuMI PshAI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmnI ZraI