(Circular) (Six-base) MAPSORT of: pFN21AB4099.seq Check: 2748 from: 1 to: 6061 pFN21AB4099 6061 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3190 3190 Size: 6061 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 2220 4188 4561 1563 Size: 657 1968 373 3063 Fragments arranged by size: 3063 1968 657 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2053 2121 2664 4124 5508 5776 1632 Size: 421 68 543 1460 1384 268 1917 Fragments arranged by size: 1917 1460 1384 543 421 268 68 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 2101 4790 2101 Size: 2689 3372 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3011 3043 3011 Size: 32 6029 AlwNI CAG_nnn'CTG Cuts at: 2855 3804 5376 2855 Size: 949 1572 3540 Fragments arranged by size: 3540 1572 949 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 2197 3370 3596 3748 5857 1493 Size: 704 1173 226 152 2109 1697 Fragments arranged by size: 2109 1697 1173 704 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 3270 3386 1870 Size: 73 1327 116 4545 Fragments arranged by size: 4545 1327 116 73 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2420 3382 4837 618 Size: 325 217 627 633 962 1455 1842 Fragments arranged by size: 1842 1455 962 633 627 325 217 BanII G_rGCy'C Cuts at: 1391 3380 1391 Size: 1989 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3070 3096 928 Size: 971 1171 26 3893 Fragments arranged by size: 3893 1171 971 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4252 4286 1903 Size: 34 2315 34 3678 Fragments arranged by size: 3678 2315 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 4122 5278 1790 Size: 2332 1156 2573 Fragments arranged by size: 2573 2332 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2656 2879 3792 Size: 416 53 83 399 24 1239 223 913 Cuts at: 3792 4750 442 Size: 958 1753 Fragments arranged by size: 1753 1239 958 913 416 399 223 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2083 2196 2594 2899 3013 4721 1239 Size: 844 113 398 305 114 1708 2579 Fragments arranged by size: 2579 1708 844 398 305 114 113 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4186 5051 5349 4186 Size: 865 298 4898 Fragments arranged by size: 4898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2941 4724 882 Size: 617 1442 1783 2219 Fragments arranged by size: 2219 1783 1442 617 BsaAI yAC'GTr Cuts at: 493 1204 3852 493 Size: 711 2648 2702 Fragments arranged by size: 2702 2648 711 BsaBI GATnn'nnATC Cuts at: 2789 2957 6055 2789 Size: 168 3098 2795 Fragments arranged by size: 3098 2795 168 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3868 4250 4885 275 Size: 53 83 186 936 2335 382 635 1451 Fragments arranged by size: 2335 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 2116 2146 3011 Size: 30 165 30 678 30 5 30 865 Cuts at: 3011 3041 1178 Size: 30 4198 Fragments arranged by size: 4198 865 678 165 30 30 30 30 30 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6061 BseYI C'CCAG_C Cuts at: 5264 5760 5264 Size: 496 5565 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5488 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2487 4122 4207 5278 2487 Size: 1635 85 1071 3270 Fragments arranged by size: 3270 1635 1071 85 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 BsmBI CGTCTCn'nnnn_ Cuts at: 3031 3031 Size: 6061 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2487 3380 4122 4207 5278 1391 Size: 399 697 893 742 85 1071 2174 Fragments arranged by size: 2174 1071 893 742 697 399 85 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 3190 3416 844 Size: 1894 452 226 3489 Fragments arranged by size: 3489 1894 452 226 BsrBI CCG'CTC Cuts at: 1394 1823 3948 1394 Size: 429 2125 3507 Fragments arranged by size: 3507 2125 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4556 4730 65 Size: 1183 3308 174 1396 Fragments arranged by size: 3308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2010 4705 4892 1700 Size: 110 200 2695 187 2869 Fragments arranged by size: 2869 2695 200 187 110 BsrGI T'GTAC_A Cuts at: 96 2561 2699 2787 96 Size: 2465 138 88 3370 Fragments arranged by size: 3370 2465 138 88 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 3099 4121 5133 3099 Size: 1022 1012 4027 Fragments arranged by size: 4027 1022 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2855 794 Size: 2061 4000 BstBI TT'CG_AA Cuts at: 2468 4865 2468 Size: 2397 3664 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstYI r'GATC_y Cuts at: 1972 3391 3758 4143 4160 5612 5698 5710 6056 Size: 1419 367 385 17 1452 86 12 346 Cuts at: 6056 1972 Size: 1977 Fragments arranged by size: 1977 1452 1419 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2271 2523 513 Size: 552 1206 252 4051 Fragments arranged by size: 4051 1206 552 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 3139 3577 4371 4391 3139 Size: 438 794 20 4809 Fragments arranged by size: 4809 794 438 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 2203 3365 2203 Size: 1162 4899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026 Size: 54 1425 50 275 18 1678 889 1629 Cuts at: 6026 8 Size: 43 Fragments arranged by size: 1678 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2673 3065 3991 1353 Size: 1320 392 926 3423 Fragments arranged by size: 3423 1320 926 392 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4648 5022 5168 387 Size: 1064 138 3059 374 146 1280 Fragments arranged by size: 3059 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2083 2121 2196 2594 2664 2899 Size: 393 421 30 38 75 398 70 235 Cuts at: 2899 3013 4124 4721 5508 5776 1239 Size: 114 1111 597 787 268 1524 Fragments arranged by size: 1524 1111 787 597 421 398 393 268 235 114 75 70 38 30 EcoO109I rG'GnC_Cy Cuts at: 2207 2207 Size: 6061 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 EcoRV GAT'ATC Cuts at: 3267 3267 Size: 6061 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 5208 1678 Size: 3530 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2087 2113 2116 2119 Size: 32 135 32 509 6 26 3 3 Cuts at: 2119 2148 3207 3239 3251 3283 4702 4734 4776 Size: 29 1059 32 12 32 1419 32 42 Cuts at: 4776 4808 1373 Size: 32 2626 Fragments arranged by size: 2626 1419 1059 509 135 42 32 32 32 32 32 32 29 26 12 6 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5175 5359 1305 Size: 3870 184 2007 Fragments arranged by size: 3870 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4239 Fragments arranged by size: 4239 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2963 3345 4019 4378 4537 518 Size: 648 1797 382 674 359 159 2042 Fragments arranged by size: 2042 1797 674 648 382 359 159 MspA1I CmG'CkG Cuts at: 1606 2572 3014 3167 3477 4154 5302 5547 1606 Size: 966 442 153 310 677 1148 245 2120 Fragments arranged by size: 2120 1148 966 677 442 310 245 153 NcoI C'CATG_G Cuts at: 513 1065 2271 2523 513 Size: 552 1206 252 4051 Fragments arranged by size: 4051 1206 552 252 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 3419 4964 1132 Size: 97 2190 1545 2229 Fragments arranged by size: 2229 2190 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 3212 1108 Size: 2104 3957 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3011 3043 4130 4162 5643 5675 3011 Size: 32 1087 32 1481 32 3397 Fragments arranged by size: 3397 1481 1087 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2207 2207 Size: 6061 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PstI C_TGCA'G Cuts at: 830 3413 830 Size: 2583 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 2572 3167 2572 Size: 595 5466 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 2488 3409 4544 5225 5416 826 Size: 219 1443 921 1135 681 191 1471 Fragments arranged by size: 1471 1443 1135 921 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3270 4165 5066 5328 820 Size: 197 926 1327 895 901 262 1553 Fragments arranged by size: 1553 1327 926 901 895 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 2233 2290 3985 5 Size: 47 2181 57 1695 2081 Fragments arranged by size: 2181 2081 1695 57 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2271 2523 3500 513 Size: 552 1206 252 977 3074 Fragments arranged by size: 3074 1206 977 552 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2915 4088 4105 4258 4443 4888 325 Size: 1255 1335 1173 17 153 185 445 1498 Fragments arranged by size: 1498 1335 1255 1173 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2561 2699 2787 Size: 274 80 33 51 1443 584 138 88 Cuts at: 2787 4307 6019 96 Size: 1520 1712 138 Fragments arranged by size: 1712 1520 1443 584 274 138 138 88 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6021 6053 6021 Size: 32 6029 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2866 3155 2866 Size: 289 5772 XhoI C'TCGA_G Cuts at: 1943 3270 1943 Size: 1327 4734 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI