(Circular) (Six-base) MAPSORT of: pFN21AB4098.seq Check: 2868 from: 1 to: 5938 pFN21AB4098 5938 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3748 278 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3281 1937 Size: 1344 4594 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2231 2238 4001 5385 5653 1632 Size: 599 7 1763 1384 268 1917 Fragments arranged by size: 1917 1763 1384 599 268 7 AfeI AGC'GCT Cuts at: 2035 2035 Size: 5938 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 4837 5814 1203 Size: 3634 977 1327 Fragments arranged by size: 3634 1327 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5938 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2880 2912 2880 Size: 32 5906 AlwNI CAG_nnn'CTG Cuts at: 2125 2290 3681 5253 2125 Size: 165 1391 1572 2810 Fragments arranged by size: 2810 1572 1391 165 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 2306 2702 3247 3473 3625 5734 1493 Size: 813 396 545 226 152 2109 1697 Fragments arranged by size: 2109 1697 813 545 396 226 152 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 2067 3263 1870 Size: 73 124 1196 4545 Fragments arranged by size: 4545 1196 124 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3175 3208 3175 Size: 33 5905 BamHI G'GATC_C Cuts at: 3268 3635 3268 Size: 367 5571 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2062 3259 4714 618 Size: 325 217 627 264 11 1197 1455 1842 Fragments arranged by size: 1842 1455 1197 627 325 264 217 11 BanII G_rGCy'C Cuts at: 1391 3173 3257 1391 Size: 1782 84 4072 Fragments arranged by size: 4072 1782 84 BbeI G_GCGC'C Cuts at: 2055 2055 Size: 5938 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2865 3099 928 Size: 971 966 234 3767 Fragments arranged by size: 3767 971 966 234 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3203 3830 5040 3203 Size: 627 1210 4101 Fragments arranged by size: 4101 1210 627 BclI T'GATC_A Cuts at: 1548 2650 1548 Size: 1102 4836 BfrBI ATG'CAT Cuts at: 2147 2147 Size: 5938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4549 136 Size: 107 122 71 631 605 337 2540 1525 Fragments arranged by size: 2540 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3043 5933 3043 Size: 2890 3048 BlpI GC'TnA_GC Cuts at: 3355 3355 Size: 5938 Bme1580I G_kGCm'C Cuts at: 1790 2067 3999 5155 1790 Size: 277 1932 1156 2573 Fragments arranged by size: 2573 1932 1156 277 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5938 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2444 3669 4627 Size: 416 53 83 399 24 1027 1225 958 Cuts at: 4627 442 Size: 1753 Fragments arranged by size: 1753 1225 1027 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2655 4598 1239 Size: 1416 1943 2579 Fragments arranged by size: 2579 1943 1416 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4063 4928 5226 4063 Size: 865 298 4775 Fragments arranged by size: 4775 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4601 882 Size: 617 3102 2219 Fragments arranged by size: 3102 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3729 493 Size: 711 2525 2702 Fragments arranged by size: 2702 2525 711 BsaBI GATnn'nnATC Cuts at: 3048 5932 3048 Size: 2884 3054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 3745 4127 4762 Size: 53 83 186 936 519 1693 382 635 Cuts at: 4762 275 Size: 1451 Fragments arranged by size: 1693 1451 936 635 519 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4371 4769 5043 5190 5601 4371 Size: 398 274 147 411 4708 Fragments arranged by size: 4708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2880 2910 1178 Size: 30 165 30 1477 30 4206 Fragments arranged by size: 4206 1477 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2901 1186 Size: 1715 4223 BseYI C'CCAG_C Cuts at: 5141 5637 5141 Size: 496 5442 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2928 1163 Size: 573 1192 4173 Fragments arranged by size: 4173 1192 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3388 4149 5177 1540 Size: 275 186 1387 761 1028 2301 Fragments arranged by size: 2301 1387 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3173 3999 4084 5155 3173 Size: 826 85 1071 3956 Fragments arranged by size: 3956 1071 826 85 BsmI GAATG_Cn' Cuts at: 2782 2980 3449 3542 2782 Size: 198 469 93 5178 Fragments arranged by size: 5178 469 198 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 3173 3257 3999 4084 5155 1391 Size: 399 277 1106 84 742 85 1071 2174 Fragments arranged by size: 2174 1106 1071 742 399 277 85 84 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 3827 5557 3827 Size: 1730 4208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3293 844 Size: 2449 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3825 1394 Size: 429 2002 3507 Fragments arranged by size: 3507 2002 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2787 4433 4607 65 Size: 1183 1539 1646 174 1396 Fragments arranged by size: 1646 1539 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2054 4582 4769 1700 Size: 110 244 2528 187 2869 Fragments arranged by size: 2869 2528 244 187 110 BsrGI T'GTAC_A Cuts at: 96 2438 96 Size: 2342 3596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5938 BstBI TT'CG_AA Cuts at: 2374 4742 2374 Size: 2368 3570 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstYI r'GATC_y Cuts at: 1972 3043 3268 3635 4020 4037 5489 5575 5587 Size: 1071 225 367 385 17 1452 86 12 Cuts at: 5587 5933 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1071 385 367 346 225 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3234 513 Size: 552 2169 3217 Fragments arranged by size: 3217 2169 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5199 Fragments arranged by size: 5199 683 56 BtsI GCAGTG_nn' Cuts at: 2616 2940 3454 4248 4268 2616 Size: 324 514 794 20 4286 Fragments arranged by size: 4286 794 514 324 20 ClaI AT'CG_AT Cuts at: 1554 2485 1554 Size: 931 5007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3210 3245 529 Size: 35 2646 35 3222 Fragments arranged by size: 3222 2646 35 35 DraI TTT'AAA Cuts at: 3242 3242 Size: 5938 DraIII CAC_nnn'GTG Cuts at: 2728 2728 Size: 5938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3231 3385 4274 Size: 54 1425 50 275 18 1401 154 889 Cuts at: 4274 5903 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1401 889 275 154 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2257 3868 1353 Size: 904 1611 3423 Fragments arranged by size: 3423 1611 904 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4525 4899 5045 387 Size: 1064 138 2936 374 146 1280 Fragments arranged by size: 2936 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3171 3171 Size: 5938 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2238 2655 4001 4598 5385 5653 Size: 393 599 7 417 1346 597 787 268 Cuts at: 5653 1239 Size: 1524 Fragments arranged by size: 1524 1346 787 599 597 417 393 268 7 EcoRI G'AATT_C Cuts at: 2306 3247 3625 2306 Size: 941 378 4619 Fragments arranged by size: 4619 941 378 EcoRV GAT'ATC Cuts at: 2224 2224 Size: 5938 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2178 2879 3166 2178 Size: 701 287 4950 Fragments arranged by size: 4950 701 287 FspI TGC'GCA Cuts at: 2030 4444 2030 Size: 2414 3524 HaeII r_GCGC'y Cuts at: 1678 2037 2055 5085 1678 Size: 359 18 3030 2531 Fragments arranged by size: 3030 2531 359 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4579 4611 4653 4685 1373 Size: 32 135 32 3007 32 42 32 2626 Fragments arranged by size: 3007 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2446 3282 3528 669 Size: 988 281 508 836 246 3079 Fragments arranged by size: 3079 988 836 508 281 246 HindIII A'AGCT_T Cuts at: 748 2681 748 Size: 1933 4005 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KasI G'GCGC_C Cuts at: 2051 2051 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 2261 3537 2261 Size: 1276 4662 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5052 5236 1305 Size: 3747 184 2007 Fragments arranged by size: 3747 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3233 10 Size: 54 1425 343 1401 2715 Fragments arranged by size: 2715 1425 1401 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2388 3896 4255 4414 518 Size: 648 1222 1508 359 159 2042 Fragments arranged by size: 2042 1508 1222 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 3354 4031 5179 5424 1606 Size: 501 1247 677 1148 245 2120 Fragments arranged by size: 2120 1247 1148 677 501 245 NaeI GCC'GGC Cuts at: 2056 2056 Size: 5938 NarI GG'CG_CC Cuts at: 2052 2052 Size: 5938 NcoI C'CATG_G Cuts at: 513 1065 3234 513 Size: 552 2169 3217 Fragments arranged by size: 3217 2169 552 NdeI CA'TA_TG Cuts at: 387 2754 387 Size: 2367 3571 NgoMIV G'CCGG_C Cuts at: 2054 2054 Size: 5938 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NsiI A_TGCA'T Cuts at: 2149 2149 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 2151 3296 4841 1132 Size: 97 922 1145 1545 2229 Fragments arranged by size: 2229 1545 1145 922 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5938 PciI A'CATG_T Cuts at: 4837 4837 Size: 5938 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5938 PfoI T'CCnGG_A Cuts at: 2969 2969 Size: 5938 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2880 2912 4007 4039 5520 5552 2880 Size: 32 1095 32 1481 32 3266 Fragments arranged by size: 3266 1481 1095 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 3508 3508 Size: 5938 PstI C_TGCA'G Cuts at: 830 3290 830 Size: 2460 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 SacI G_AGCT'C Cuts at: 3173 3173 Size: 5938 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 3032 4186 5898 3032 Size: 1154 1712 3072 Fragments arranged by size: 3072 1712 1154 SfcI C'TryA_G Cuts at: 826 1045 2092 3117 3286 4421 5102 5293 826 Size: 219 1047 1025 169 1135 681 191 1471 Fragments arranged by size: 1471 1135 1047 1025 681 219 191 169 SfoI GGC'GCC Cuts at: 2053 2053 Size: 5938 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4042 4943 5205 820 Size: 197 926 2099 901 262 1553 Fragments arranged by size: 2099 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SspI AAT'ATT Cuts at: 5 52 2474 3862 5 Size: 47 2422 1388 2081 Fragments arranged by size: 2422 2081 1388 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5938 StyI C'CwwG_G Cuts at: 513 1065 3234 3377 513 Size: 552 2169 143 3074 Fragments arranged by size: 3074 2169 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3965 3982 4135 4320 4765 325 Size: 1255 2385 17 153 185 445 1498 Fragments arranged by size: 2385 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2438 3030 4184 Size: 274 80 33 51 1443 461 592 1154 Cuts at: 4184 5896 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1154 592 461 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2632 2664 5898 5930 2632 Size: 32 3234 32 2640 Fragments arranged by size: 3234 2640 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2450 1316 Size: 1134 4804 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 3126 4067 3126 Size: 941 4997 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3746 276 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I FseI FspAI NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI