Restriction map for
pFN21AB4086
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BanII BbeI
BbrPI BcgI BciVI BfrI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FseI FspI HaeII HhaI HinP1I
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 1403 GGCGTC G[GA]^CG[CT]C
Asp700I 840 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 37 TGGCCA TGG^CCA
BanI 1254 GGCACC G^G[CT][GA]CC
BbvCI 1084 CCTCAGC CCTCAGC(-5/-2)
BclI 93 TGATCA T^GATCA
BcnI 1180 CCGGG CC^[GC]GG
BglII 68 AGATCT A^GATCT
BsaHI 1403 GGCGTC G[GA]^CG[CT]C
BsgI 174 CTGCAC GTGCAG(16/14)
BsmAI 283 GAGAC GTCTC(1/5)
Bsp1407I 1031 TGTACA T^GTACA
BspHI 779 TCATGA T^CATGA
BsrGI 1031 TGTACA T^GTACA
Bst1107I 138 GTATAC GTA^TAC
BstXI 143 CCATTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 138 GTATAC GTA^TAC
EaeI 37 TGGCCA [CT]^GGCC[GA]
EcoT22I 380 ATGCAT ATGCA^T
FbaI 93 TGATCA T^GATCA
HapII 1180 CCGG C^CGG
HgaI 1404 GCGTC GACGC(5/10)
Hin1I 1403 GGCGTC G[GA]^CG[CT]C
HindIII 29 AAGCTT A^AGCTT
HpaI 418 GTTAAC GTT^AAC
HpaII 1180 CCGG C^CGG
MaeII 1354 ACGT A^CGT
MaeIII 1162 GTCAC ^GT[ACGT]AC
MluNI 37 TGGCCA TGG^CCA
MscI 37 TGGCCA TGG^CCA
MspI 1180 CCGG C^CGG
NciI 1180 CCGGG CC^[GC]GG
NdeI 589 CATATG CA^TATG
NheI 964 GCTAGC G^CTAGC
NsiI 380 ATGCAT ATGCA^T
RcaI 779 TCATGA T^CATGA
ScaI 1076 AGTACT AGT^ACT
SmlI 64 CTTGAG C^T[CT][GA]AG
SphI 382 GCATGC GCATG^C
SspBI 1031 TGTACA T^GTACA
Tsp45I 1162 GTCAC ^GT[GC]AC
XmnI 840 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 138 GTATAC GT^[AC][GT]AC
703 GTCTAC
AciI 690 CCGC CCGC(-3/-1)
264 GCGG
AlwI 717 GATCC GGATC(4/5)
7 GGATC
AlwNI 398 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1523 CAGAACCTG
BsmFI 583 GGGAC GGGAC(10/14)
1406 GTCCC
BsmI 21 GAATGC GAATGC(1/-1)
1452 GCATTC
BstYI 68 AGATCT [GA]^GATC[CT]
716 AGATCC
Cfr13I 153 GGGCC G^G[ACGT]CC
544 GGGCC
HincII 418 GTTAAC GT[CT]^[GA]AC
1309 GTCAAC
HindII 418 GTTAAC GT[CT]^[GA]AC
1309 GTCAAC
HphI 793 TCACC GGTGA(8/7)
1081 TCACC
MflI 68 AGATCT [GA]^GATC[CT]
716 AGATCC
MslI 207 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
598 CATGCCTATG
NlaIV 1051 GGTTCC GG[ACGT]^[ACGT]CC
1254 GGCACC
NspI 382 GCATGC [GA]CATG^[CT]
597 ACATGC
PflMI 434 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1299 CCATCATTTGG
Sau96I 153 GGGCC G^G[ACGT]CC
544 GGGCC
TaqI 293 TCGA T^CGA
368 TCGA
TthHB8I 293 TCGA T^CGA
368 TCGA
Van91I 434 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1299 CCATCATTTGG
XhoII 68 AGATCT [GA]^GATC[CT]
716 AGATCC
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI BbsI BbvI BfaI
BpuAI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII PleI
PvuII RsaI Sau3AI ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp509I
TspRI