(Circular) (Six-base) MAPSORT of: pFN21AB4086.seq Check: 5215 from: 1 to: 6265 pFN21AB4086 6265 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2144 2709 3608 1937 Size: 207 565 899 4594 Fragments arranged by size: 4594 899 565 207 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2968 3030 3555 4328 5712 5980 1632 Size: 1336 62 525 773 1384 268 1917 Fragments arranged by size: 1917 1384 1336 773 525 268 62 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 3562 5164 6141 1203 Size: 2359 1602 977 1327 Fragments arranged by size: 2359 1602 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2053 1184 Size: 869 5396 AlwNI CAG_nnn'CTG Cuts at: 2408 3533 4008 5580 2408 Size: 1125 475 1572 3093 Fragments arranged by size: 3093 1572 1125 475 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 3590 1870 Size: 73 1647 4545 Fragments arranged by size: 4545 1647 73 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3259 3586 5041 618 Size: 325 217 627 1472 327 1455 1842 Fragments arranged by size: 1842 1472 1455 627 327 325 217 BanII G_rGCy'C Cuts at: 1391 3584 1391 Size: 2193 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2324 2404 2931 3262 928 Size: 971 425 80 527 331 3931 Fragments arranged by size: 3931 971 527 425 331 80 BbvCI CC'TCA_GC Cuts at: 3090 3090 Size: 6265 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4456 4490 1903 Size: 34 50 34 2435 34 3678 Fragments arranged by size: 3678 2435 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 2098 1548 Size: 550 5715 BfrBI ATG'CAT Cuts at: 2387 2387 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4876 136 Size: 107 122 71 631 605 3204 1525 Fragments arranged by size: 3204 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2073 6260 2073 Size: 4187 2078 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 4326 5482 1790 Size: 2536 1156 2573 Fragments arranged by size: 2573 2536 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2725 3996 4954 Size: 416 53 83 399 24 1308 1271 958 Cuts at: 4954 442 Size: 1753 Fragments arranged by size: 1753 1308 1271 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2973 1056 Size: 1917 4348 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2458 2458 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4925 1239 Size: 3686 2579 Bpu10I CC'TnA_GC Cuts at: 3090 3090 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2090 4390 5255 5553 2090 Size: 2300 865 298 2802 Fragments arranged by size: 2802 2300 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3409 4072 4454 5089 Size: 53 83 186 936 1876 663 382 635 Cuts at: 5089 275 Size: 1451 Fragments arranged by size: 1876 1451 936 663 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3495 3525 1178 Size: 30 165 30 2092 30 3918 Fragments arranged by size: 3918 2092 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6265 BseYI C'CCAG_C Cuts at: 5468 5964 5468 Size: 496 5769 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2164 1163 Size: 573 428 5264 Fragments arranged by size: 5264 573 428 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4326 4411 5482 4326 Size: 85 1071 5109 Fragments arranged by size: 5109 1071 85 BsmI GAATG_Cn' Cuts at: 2032 3457 3776 3869 2032 Size: 1425 319 93 4428 Fragments arranged by size: 4428 1425 319 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3584 4326 4411 5482 1391 Size: 399 1794 742 85 1071 2174 Fragments arranged by size: 2174 1794 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 2784 4154 5884 2784 Size: 1370 1730 3165 Fragments arranged by size: 3165 1730 1370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3620 844 Size: 2776 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4152 1394 Size: 429 2329 3507 Fragments arranged by size: 3507 2329 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4909 5096 1700 Size: 110 3099 187 2869 Fragments arranged by size: 3099 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3036 96 Size: 2940 3325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6265 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstXI CCAn_nnnn'nTGG Cuts at: 2155 2155 Size: 6265 BstYI r'GATC_y Cuts at: 1972 2073 2721 3595 3962 4347 4364 5816 5902 Size: 101 648 874 367 385 17 1452 86 Cuts at: 5902 5914 6260 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 874 648 385 367 346 101 86 17 12 BstZ17I GTA'TAC Cuts at: 2145 2145 Size: 6265 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5713 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 3781 4575 4595 3781 Size: 794 20 5451 Fragments arranged by size: 5451 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2034 2069 529 Size: 35 1470 35 4725 Fragments arranged by size: 4725 1470 35 35 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2042 3712 4601 Size: 54 1425 50 275 18 212 1670 889 Cuts at: 4601 6230 8 Size: 1629 43 Fragments arranged by size: 1670 1629 1425 889 275 212 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2337 2987 3101 4195 1353 Size: 984 650 114 1094 3423 Fragments arranged by size: 3423 1094 984 650 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4852 5226 5372 387 Size: 1064 138 3263 374 146 1280 Fragments arranged by size: 3263 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2968 3030 3555 4328 4925 5712 5980 Size: 393 1336 62 525 773 597 787 268 Cuts at: 5980 1239 Size: 1524 Fragments arranged by size: 1524 1336 787 773 597 525 393 268 62 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2191 2191 Size: 6265 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 5412 1678 Size: 3734 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2618 2650 3215 Size: 32 135 32 614 32 400 32 565 Cuts at: 3215 3247 4906 4938 4980 5012 1373 Size: 32 1659 32 42 32 2626 Fragments arranged by size: 2626 1659 614 565 400 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 3316 3609 3855 669 Size: 988 281 487 891 293 246 3079 Fragments arranged by size: 3079 988 891 487 293 281 246 HindIII A'AGCT_T Cuts at: 748 2034 748 Size: 1286 4979 HpaI GTT'AAC Cuts at: 2425 3855 2425 Size: 1430 4835 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 2260 2506 5379 5563 1305 Size: 786 169 246 2873 184 2007 Fragments arranged by size: 2873 2007 786 246 184 169 MscI TGG'CCA Cuts at: 10 64 1489 1832 2044 10 Size: 54 1425 343 212 4231 Fragments arranged by size: 4231 1425 343 212 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2607 4223 4582 4741 518 Size: 648 1050 391 1616 359 159 2042 Fragments arranged by size: 2042 1616 1050 648 391 359 159 MspA1I CmG'CkG Cuts at: 1606 2335 2405 2697 3542 3681 4358 5506 5751 Size: 729 70 292 845 139 677 1148 245 Cuts at: 5751 1606 Size: 2120 Fragments arranged by size: 2120 1148 845 729 677 292 245 139 70 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5713 NdeI CA'TA_TG Cuts at: 387 2595 387 Size: 2208 4057 NheI G'CTAG_C Cuts at: 1052 2969 1052 Size: 1917 4348 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NsiI A_TGCA'T Cuts at: 2389 2389 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2391 2606 3566 3623 5168 1132 Size: 97 1162 215 960 57 1545 2229 Fragments arranged by size: 2229 1545 1162 960 215 97 57 PasI CC'CwG_GG Cuts at: 1348 3328 1348 Size: 1980 4285 PciI A'CATG_T Cuts at: 3562 5164 3562 Size: 1602 4663 PflMI CCAn_nnn'nTGG Cuts at: 1108 2445 3310 1108 Size: 1337 865 4063 Fragments arranged by size: 4063 1337 865 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3388 3420 4334 4366 5847 5879 3388 Size: 32 914 32 1481 32 3774 Fragments arranged by size: 3774 1481 914 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PstI C_TGCA'G Cuts at: 830 3617 830 Size: 2787 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2335 2405 3542 2335 Size: 70 1137 5058 Fragments arranged by size: 5058 1137 70 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 3083 4513 6225 3083 Size: 1430 1712 3123 Fragments arranged by size: 3123 1712 1430 SfcI C'TryA_G Cuts at: 826 1045 2514 2646 2887 3613 4748 5429 5620 Size: 219 1469 132 241 726 1135 681 191 Cuts at: 5620 826 Size: 1471 Fragments arranged by size: 1471 1469 1135 726 681 241 219 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 4369 5270 5532 820 Size: 197 926 126 2300 901 262 1553 Fragments arranged by size: 2300 1553 926 901 262 197 126 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 2391 3623 1132 Size: 1259 1232 3774 Fragments arranged by size: 3774 1259 1232 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6265 StyI C'CwwG_G Cuts at: 513 1065 3704 513 Size: 552 2639 3074 Fragments arranged by size: 3074 2639 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3317 4292 4309 4462 4647 5092 325 Size: 1255 1737 975 17 153 185 445 1498 Fragments arranged by size: 1737 1498 1255 975 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3036 3081 3153 Size: 274 80 33 51 1443 1059 45 72 Cuts at: 3153 4511 6223 96 Size: 1358 1712 138 Fragments arranged by size: 1712 1443 1358 1059 274 138 80 72 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 2849 4394 2849 Size: 1545 4720 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI