(Circular) (Six-base) MAPSORT of: pFN21AB4080.seq Check: 6130 from: 1 to: 5719 pFN21AB4080 5719 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3529 278 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2680 3062 1937 Size: 743 382 4594 Fragments arranged by size: 4594 743 382 Acc65I G'GTAC_C Cuts at: 2618 3040 2618 Size: 422 5297 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3782 5166 5434 1632 Size: 2150 1384 268 1917 Fragments arranged by size: 2150 1917 1384 268 AfeI AGC'GCT Cuts at: 2309 2309 Size: 5719 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 2088 2142 4618 5595 1203 Size: 885 54 2476 977 1327 Fragments arranged by size: 2476 1327 977 885 54 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 4448 4448 Size: 5719 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5719 AlwNI CAG_nnn'CTG Cuts at: 3462 5034 3462 Size: 1572 4147 ApaLI G'TGCA_C Cuts at: 3776 4932 3776 Size: 1156 4563 ApoI r'AATT_y Cuts at: 1493 2246 2508 2517 2630 2781 2920 3028 3254 Size: 753 262 9 113 151 139 108 226 Cuts at: 3254 3406 5515 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 753 262 226 152 151 139 113 108 9 AseI AT'TA_AT Cuts at: 160 1032 2595 4273 160 Size: 872 1563 1678 1606 Fragments arranged by size: 1678 1606 1563 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 2844 3044 1870 Size: 73 901 200 4545 Fragments arranged by size: 4545 901 200 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2394 2427 2394 Size: 33 5686 BamHI G'GATC_C Cuts at: 3049 3416 3049 Size: 367 5352 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2618 3040 4495 618 Size: 325 217 627 831 422 1455 1842 Fragments arranged by size: 1842 1455 831 627 422 325 217 BanII G_rGCy'C Cuts at: 1391 2326 3038 1391 Size: 935 712 4072 Fragments arranged by size: 4072 935 712 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2486 928 Size: 971 587 4161 Fragments arranged by size: 4161 971 587 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3910 3944 1903 Size: 34 1973 34 3678 Fragments arranged by size: 3678 1973 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2149 3611 4821 2149 Size: 1462 1210 3047 Fragments arranged by size: 3047 1462 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4330 136 Size: 107 122 71 631 605 2658 1525 Fragments arranged by size: 2658 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2961 5714 2961 Size: 2753 2966 BlpI GC'TnA_GC Cuts at: 2562 3136 2562 Size: 574 5145 Bme1580I G_kGCm'C Cuts at: 1790 3780 4936 1790 Size: 1990 1156 2573 Fragments arranged by size: 2573 1990 1156 BmgBI CAC'GTC Cuts at: 1318 2091 1318 Size: 773 4946 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2095 2398 3450 Size: 416 53 83 399 24 678 303 1052 Cuts at: 3450 4408 442 Size: 958 1753 Fragments arranged by size: 1753 1052 958 678 416 399 303 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4379 1239 Size: 3140 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2391 2552 3844 4709 5007 2391 Size: 161 1292 865 298 3103 Fragments arranged by size: 3103 1292 865 298 161 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4382 882 Size: 617 2883 2219 Fragments arranged by size: 2883 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3510 493 Size: 711 2306 2702 Fragments arranged by size: 2702 2306 711 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3526 3908 4543 275 Size: 53 83 186 936 1993 382 635 1451 Fragments arranged by size: 1993 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4152 4550 4824 4971 5382 4152 Size: 398 274 147 411 4489 Fragments arranged by size: 4489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2861 2891 1178 Size: 30 165 30 1458 30 4006 Fragments arranged by size: 4006 1458 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5719 BseYI C'CCAG_C Cuts at: 4922 5418 4922 Size: 496 5223 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2417 1163 Size: 573 681 4465 Fragments arranged by size: 4465 681 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3169 3930 4958 1540 Size: 275 186 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3780 3865 4936 3780 Size: 85 1071 4563 Fragments arranged by size: 4563 1071 85 BsmI GAATG_Cn' Cuts at: 2649 3230 3323 2649 Size: 581 93 5045 Fragments arranged by size: 5045 581 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2326 3038 3780 3865 4936 1391 Size: 399 536 712 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 712 536 399 85 BspEI T'CCGG_A Cuts at: 5382 5382 Size: 5719 BspHI T'CATG_A Cuts at: 3608 5338 3608 Size: 1730 3989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3074 844 Size: 2230 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3606 1394 Size: 429 1783 3507 Fragments arranged by size: 3507 1783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4214 4388 65 Size: 1183 2966 174 1396 Fragments arranged by size: 2966 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4363 4550 1700 Size: 110 2553 187 2869 Fragments arranged by size: 2869 2553 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 3779 4791 3779 Size: 1012 4707 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5719 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5719 BstXI CCAn_nnnn'nTGG Cuts at: 2388 2786 2388 Size: 398 5321 BstYI r'GATC_y Cuts at: 1972 2961 3049 3416 3801 3818 5270 5356 5368 Size: 989 88 367 385 17 1452 86 12 Cuts at: 5368 5714 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 989 385 367 346 88 86 17 12 BstZ17I GTA'TAC Cuts at: 2681 2681 Size: 5719 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5167 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4980 Fragments arranged by size: 4980 683 56 BtsI GCAGTG_nn' Cuts at: 2188 2632 3235 4029 4049 2188 Size: 444 603 794 20 3858 Fragments arranged by size: 3858 794 603 444 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3166 4055 5684 Size: 54 1425 50 275 18 1336 889 1629 Cuts at: 5684 8 Size: 43 Fragments arranged by size: 1629 1425 1336 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3166 1537 Size: 275 1354 4090 Fragments arranged by size: 4090 1354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3649 1353 Size: 2296 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4306 4680 4826 387 Size: 1064 138 2717 374 146 1280 Fragments arranged by size: 2717 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3782 4379 5166 5434 1239 Size: 393 2150 597 787 268 1524 Fragments arranged by size: 2150 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2508 2517 3028 3406 2508 Size: 9 511 378 4821 Fragments arranged by size: 4821 511 378 9 EcoRV GAT'ATC Cuts at: 2191 2191 Size: 5719 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2281 2326 2281 Size: 45 5674 FspI TGC'GCA Cuts at: 4225 4225 Size: 5719 HaeII r_GCGC'y Cuts at: 1678 2311 4866 1678 Size: 633 2555 2531 Fragments arranged by size: 2555 2531 633 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2473 2505 4360 4392 4434 Size: 32 135 32 901 32 1855 32 42 Cuts at: 4434 4466 1373 Size: 32 2626 Fragments arranged by size: 2626 1855 901 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3063 3309 669 Size: 988 281 1125 246 3079 Fragments arranged by size: 3079 1125 988 281 246 HindIII A'AGCT_T Cuts at: 748 2200 748 Size: 1452 4267 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KpnI G_GTAC'C Cuts at: 2622 3044 2622 Size: 422 5297 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4833 5017 1305 Size: 3528 184 2007 Fragments arranged by size: 3528 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3897 Fragments arranged by size: 3897 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2375 3677 4036 4195 518 Size: 648 1209 1302 359 159 2042 Fragments arranged by size: 2042 1302 1209 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2806 2934 3135 3812 4960 5205 1606 Size: 1200 128 201 677 1148 245 2120 Fragments arranged by size: 2120 1200 1148 677 245 201 128 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5167 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 2146 2890 3077 4622 1132 Size: 97 917 744 187 1545 2229 Fragments arranged by size: 2229 1545 917 744 187 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5719 PciI A'CATG_T Cuts at: 2142 4618 2142 Size: 2476 3243 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5719 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PspXI vC'TCGA_Gb Cuts at: 2844 2844 Size: 5719 PstI C_TGCA'G Cuts at: 830 3071 830 Size: 2241 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 PvuII CAG'CTG Cuts at: 2934 2934 Size: 5719 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 3002 3967 5679 3002 Size: 965 1712 3042 Fragments arranged by size: 3042 1712 965 SfcI C'TryA_G Cuts at: 826 1045 3067 4202 4883 5074 826 Size: 219 2022 1135 681 191 1471 Fragments arranged by size: 2022 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 3046 1872 Size: 1174 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2406 2567 2844 3823 4724 4986 Size: 197 926 463 161 277 979 901 262 Cuts at: 4986 820 Size: 1553 Fragments arranged by size: 1553 979 926 901 463 277 262 197 161 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 3077 1132 Size: 1945 3774 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5719 StyI C'CwwG_G Cuts at: 513 1065 3158 513 Size: 552 2093 3074 Fragments arranged by size: 3074 2093 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3746 3763 3916 4101 4546 325 Size: 1255 2166 17 153 185 445 1498 Fragments arranged by size: 2166 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3000 3965 5677 Size: 274 80 33 51 1443 1023 965 1712 Cuts at: 5677 96 Size: 138 Fragments arranged by size: 1712 1443 1023 965 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5679 5711 5679 Size: 32 5687 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XhoI C'TCGA_G Cuts at: 1943 2844 1943 Size: 901 4818 XmaI C'CCGG_G Cuts at: 1870 3044 1870 Size: 1174 4545 XmnI GAAnn'nnTTC Cuts at: 2774 3848 2774 Size: 1074 4645 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3527 276 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI