(Circular) (Six-base) MAPSORT of: pFN21AB4055.seq Check: 4124 from: 1 to: 5704 pFN21AB4055 5704 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2551 3514 278 Size: 53 83 186 936 1015 963 2468 Fragments arranged by size: 2468 1015 963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3047 1937 Size: 1110 4594 Acc65I G'GTAC_C Cuts at: 3025 3025 Size: 5704 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2685 2774 3767 5151 5419 1632 Size: 1053 89 993 1384 268 1917 Fragments arranged by size: 1917 1384 1053 993 268 89 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 4603 5580 1203 Size: 3400 977 1327 Fragments arranged by size: 3400 1327 977 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 2592 4433 2592 Size: 1841 3863 AleI CACnn'nnGTG Cuts at: 1166 2738 1166 Size: 1572 4132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2284 1184 Size: 1100 4604 AlwNI CAG_nnn'CTG Cuts at: 3447 5019 3447 Size: 1572 4132 ApaLI G'TGCA_C Cuts at: 3761 4917 3761 Size: 1156 4548 ApoI r'AATT_y Cuts at: 1493 2110 2126 2330 2351 2378 3013 3239 3391 Size: 617 16 204 21 27 635 226 152 Cuts at: 3391 5500 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 635 617 226 204 152 27 21 16 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 3029 1870 Size: 73 1086 4545 Fragments arranged by size: 4545 1086 73 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2940 3025 4480 618 Size: 325 217 627 1153 85 1455 1842 Fragments arranged by size: 1842 1455 1153 627 325 217 85 BanII G_rGCy'C Cuts at: 1391 2862 2981 3023 1391 Size: 1471 119 42 4072 Fragments arranged by size: 4072 1471 119 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2893 928 Size: 971 994 3739 Fragments arranged by size: 3739 994 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3895 3929 1903 Size: 34 1958 34 3678 Fragments arranged by size: 3678 1958 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 1548 Size: 5704 BfrBI ATG'CAT Cuts at: 2709 2709 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4315 136 Size: 107 122 71 631 605 2643 1525 Fragments arranged by size: 2643 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5699 5699 Size: 5704 BlpI GC'TnA_GC Cuts at: 2810 3121 2810 Size: 311 5393 Bme1580I G_kGCm'C Cuts at: 1790 2943 3765 4921 1790 Size: 1153 822 1156 2573 Fragments arranged by size: 2573 1156 1153 822 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2856 3435 4393 Size: 416 53 83 399 24 1439 579 958 Cuts at: 4393 442 Size: 1753 Fragments arranged by size: 1753 1439 958 579 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4364 1239 Size: 3125 2579 Bpu10I CC'TnA_GC Cuts at: 2882 2882 Size: 5704 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3829 4694 4992 3829 Size: 865 298 4541 Fragments arranged by size: 4541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 882 Size: 617 2868 2219 Fragments arranged by size: 2868 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3495 493 Size: 711 2291 2702 Fragments arranged by size: 2702 2291 711 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2548 3511 3893 4528 Size: 53 83 186 936 1015 963 382 635 Cuts at: 4528 275 Size: 1451 Fragments arranged by size: 1451 1015 963 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2760 4137 4535 4809 4956 5367 2760 Size: 1377 398 274 147 411 3097 Fragments arranged by size: 3097 1377 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5479 Fragments arranged by size: 5479 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2779 1186 Size: 1102 491 4111 Fragments arranged by size: 4111 1102 491 BseYI C'CCAG_C Cuts at: 2040 4907 5403 2040 Size: 2867 496 2341 Fragments arranged by size: 2867 2341 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5131 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3154 3915 4943 1540 Size: 275 186 1153 761 1028 2301 Fragments arranged by size: 2301 1153 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3765 3850 4921 3765 Size: 85 1071 4548 Fragments arranged by size: 4548 1071 85 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2862 2943 2981 3023 3765 3850 4921 Size: 399 1072 81 38 42 742 85 1071 Cuts at: 4921 1391 Size: 2174 Fragments arranged by size: 2174 1072 1071 742 399 85 81 42 38 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 2487 3593 5323 2487 Size: 1106 1730 2868 Fragments arranged by size: 2868 1730 1106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3059 844 Size: 2215 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3591 1394 Size: 429 1768 3507 Fragments arranged by size: 3507 1768 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4348 4535 1700 Size: 110 2538 187 2869 Fragments arranged by size: 2869 2538 187 110 BsrGI T'GTAC_A Cuts at: 96 2949 96 Size: 2853 2851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5704 BssSI C'ACGA_G Cuts at: 3764 4776 3764 Size: 1012 4692 BstAPI GCAn_nnn'nTGC Cuts at: 794 2513 2696 794 Size: 1719 183 3802 Fragments arranged by size: 3802 1719 183 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstYI r'GATC_y Cuts at: 1972 2481 2536 3034 3401 3786 3803 5255 5341 Size: 509 55 498 367 385 17 1452 86 Cuts at: 5341 5353 5699 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 509 498 385 367 346 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4213 Fragments arranged by size: 4213 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 3220 4014 4034 3220 Size: 794 20 4890 Fragments arranged by size: 4890 794 20 ClaI AT'CG_AT Cuts at: 1554 2084 1554 Size: 530 5174 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DraIII CAC_nnn'GTG Cuts at: 2947 2947 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2803 4711 809 Size: 507 1487 1908 1802 Fragments arranged by size: 1908 1802 1487 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2215 2820 3151 Size: 54 1425 50 275 18 385 605 331 Cuts at: 3151 4040 5669 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 605 385 331 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3151 1537 Size: 275 1339 4090 Fragments arranged by size: 4090 1339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3634 1353 Size: 2281 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4291 4665 4811 387 Size: 1064 138 2702 374 146 1280 Fragments arranged by size: 2702 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2685 2774 3767 4364 5151 5419 1239 Size: 393 1053 89 993 597 787 268 1524 Fragments arranged by size: 1524 1053 993 787 597 393 268 89 EcoNI CCTnn'n_nnAGG Cuts at: 2139 2139 Size: 5704 EcoO109I rG'GnC_Cy Cuts at: 2434 2434 Size: 5704 EcoRI G'AATT_C Cuts at: 2110 2351 3013 3391 2110 Size: 241 662 378 4423 Fragments arranged by size: 4423 662 378 241 EcoRV GAT'ATC Cuts at: 2326 2326 Size: 5704 FspI TGC'GCA Cuts at: 4210 4210 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 2643 4851 1678 Size: 965 2208 2531 Fragments arranged by size: 2531 2208 965 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2578 2610 2697 2729 4345 Size: 32 135 32 1006 32 87 32 1616 Cuts at: 4345 4377 4419 4451 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1616 1006 135 87 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3048 3294 669 Size: 988 281 1110 246 3079 Fragments arranged by size: 3079 1110 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KpnI G_GTAC'C Cuts at: 3029 3029 Size: 5704 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2476 2564 4818 5002 1305 Size: 1171 88 2254 184 2007 Fragments arranged by size: 2254 2007 1171 184 88 MscI TGG'CCA Cuts at: 10 64 1489 1832 2217 2822 10 Size: 54 1425 343 385 605 2892 Fragments arranged by size: 2892 1425 605 385 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2738 3662 4021 4180 518 Size: 648 843 729 924 359 159 2042 Fragments arranged by size: 2042 924 843 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2838 2928 3120 3797 4945 5190 1606 Size: 1232 90 192 677 1148 245 2120 Fragments arranged by size: 2120 1232 1148 677 245 192 90 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4213 Fragments arranged by size: 4213 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NsiI A_TGCA'T Cuts at: 2711 2711 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 3062 4607 1132 Size: 97 1833 1545 2229 Fragments arranged by size: 2229 1833 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5704 PfoI T'CCnGG_A Cuts at: 2958 2958 Size: 5704 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 2232 3274 2232 Size: 1042 4662 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2978 3010 2978 Size: 32 5672 PstI C_TGCA'G Cuts at: 830 3056 830 Size: 2226 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 2036 3952 5664 2036 Size: 1916 1712 2076 Fragments arranged by size: 2076 1916 1712 SfcI C'TryA_G Cuts at: 826 1045 3052 4187 4868 5059 826 Size: 219 2007 1135 681 191 1471 Fragments arranged by size: 2007 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 3031 1872 Size: 1159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3808 4709 4971 820 Size: 197 926 1865 901 262 1553 Fragments arranged by size: 1865 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 2228 3628 5 Size: 47 2176 1400 2081 Fragments arranged by size: 2176 2081 1400 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5704 StyI C'CwwG_G Cuts at: 513 1065 2004 3143 513 Size: 552 939 1139 3074 Fragments arranged by size: 3074 1139 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2842 3731 3748 3901 4086 4531 325 Size: 1255 1262 889 17 153 185 445 1498 Fragments arranged by size: 1498 1262 1255 889 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2034 2949 3950 Size: 274 80 33 51 1443 57 915 1001 Cuts at: 3950 5662 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1001 915 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2012 2044 5664 5696 2012 Size: 32 3620 32 2020 Fragments arranged by size: 3620 2020 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2897 1316 Size: 1581 4123 XbaI T'CTAG_A Cuts at: 2374 3040 2374 Size: 666 5038 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 3029 1870 Size: 1159 4545 XmnI GAAnn'nnTTC Cuts at: 2581 3833 2581 Size: 1252 4452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2549 3512 276 Size: 53 83 186 936 1015 963 2468 Fragments arranged by size: 2468 1015 963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI