Restriction map for
pFN21AB4017
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AlwI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbrPI BbvCI BciVI BfrI
BlnI BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MluI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SspBI SspI SwaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 1043 TCCGGA T^CCGGA
AflIII 1085 ACGTGT A^C[GA][CT]GT
AocI 1014 CCTCAGG CC^T[ACGT]AGG
Aor51HI 578 AGCGCT AGC^GCT
ApaI 81 GGGCCC GGGCC^C
AspI 115 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 101 GGCGCC GGCGC^C
BcgI 807 CGACTTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1307 TGATCA T^GATCA
BfaI 37 CTAG C^TAG
BlpI 688 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 688 GCTGAGC GC^T[ACGT]AGC
BsaI 43 GAGACC GGTCTC(1/5)
BsaWI 1043 TCCGGA [AT]^CCGG[AT]
BseAI 1043 TCCGGA T^CCGGA
BsmAI 43 GAGAC GTCTC(1/5)
BspEI 1043 TCCGGA T^CCGGA
BsrBI 51 CCGCTC CCGCTC(-3/-3)
BsrDI 478 CATTGC GCAATG(2/0)
Bsu36I 1014 CCTCAGG CC^T[ACGT]AGG
CelII 688 GCTGAGC GC^T[ACGT]AGC
DsaI 93 CCACGG C^C[GA][CT]GG
Eco47III 578 AGCGCT AGC^GCT
Eco81I 1014 CCTCAGG CC^T[ACGT]AGG
EcoNI 1315 CCTAACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1307 TGATCA T^GATCA
HgaI 1140 GACGC GACGC(5/10)
KasI 101 GGCGCC G^GCGCC
MaeI 37 CTAG C^TAG
MfeI 819 CAATTG C^AATTG
MroI 1043 TCCGGA T^CCGGA
MseI 1252 TTAA T^TAA
MunI 819 CAATTG C^AATTG
NaeI 698 GCCGGC GCC^GGC
NarI 101 GGCGCC GG^CGCC
NgoMIV 698 GCCGGC G^CCGGC
PflFI 115 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 477 CCATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 931 GACTC GAGTC(4/5)
PshAI 1185 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 657 GCTCTTC GCTCTTC(1/4)
SbfI 75 CCTGCAGG CCTGCA^GG
SfiI 1113 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 101 GGCGCC GGC^GCC
SphI 653 GCATGC GCATG^C
Sse8387I 75 CCTGCAGG CCTGCA^GG
TfiI 148 GAATC G^A[AT]TC
Tru9I 1252 TTAA T^TAA
Tth111I 115 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 477 CCATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 36 TCTAGA T^CTAGA
XcmI 941 CCACTGGCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccII 199 CGCG CG^CG
559 CGCG
AcyI 101 GGCGCC G[GA]^CG[CT]C
1140 GACGCC
AspHI 88 GAGCTC G[AT]GC[AT]^C
1270 GAGCTC
AvaI 85 CCCGAG C^[CT]CG[GA]G
1127 CTCGGG
BalI 59 TGGCCA TGG^CCA
218 TGGCCA
BanI 101 GGCGCC G^G[CT][GA]CC
630 GGTGCC
BbsI 1137 GAAGAC GAAGAC(2/6)
16 GTCTTC
BglII 320 AGATCT A^GATCT
563 AGATCT
BpmI 933 CTCCAG CTGGAG(16/14)
327 CTGGAG
BpuAI 1137 GAAGAC GAAGAC(2/6)
16 GTCTTC
BsaHI 101 GGCGCC G[GA]^CG[CT]C
1140 GACGCC
BsgI 1203 CTGCAC GTGCAG(16/14)
966 GTGCAG
BsiEI 201 CGACCG CG[GA][CT]^CG
624 CGACCG
BsiHKAI 88 GAGCTC G[AT]GC[AT]^C
1270 GAGCTC
BsoBI 85 CCCGAG C^[CT]CG[GA]G
1127 CTCGGG
BspMI 758 ACCTGC ACCTGC(4/8)
1201 ACCTGC
BsrFI 387 GCCGGT [GA]^CCGG[CT]
698 GCCGGC
BstAPI 309 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
473 GCAACCATTGC
BstEII 390 GGTGACC G^GT[ACGT]ACC
843 GGTCACC
BstPI 390 GGTGACC G^GT[ACGT]ACC
843 GGTCACC
BstUI 199 CGCG CG^CG
559 CGCG
BstXI 746 CCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1056 CCAGGCCTTTGG
BstYI 320 AGATCT [GA]^GATC[CT]
563 AGATCT
Cfr10I 387 GCCGGT [GA]^CCGG[CT]
698 GCCGGC
DrdI 451 GACCCGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1356 GACCGTGCTGTC
EarI 25 CTCTTC CTCTTC(1/4)
658 CTCTTC
EcoO65I 390 GGTGACC G^GT[ACGT]ACC
843 GGTCACC
EcoT14I 242 CCTTGG C^C[AT][AT]GG
494 CCAAGG
Hin1I 101 GGCGCC G[GA]^CG[CT]C
1140 GACGCC
HinfI 148 GAATC G^A[ACGT]TC
931 GACTC
Ksp632I 25 CTCTTC CTCTTC(1/4)
658 CTCTTC
MflI 320 AGATCT [GA]^GATC[CT]
563 AGATCT
MluNI 59 TGGCCA TGG^CCA
218 TGGCCA
MscI 59 TGGCCA TGG^CCA
218 TGGCCA
MslI 948 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1074 CACGGACGTG
MvnI 199 CGCG CG^CG
559 CGCG
NspI 653 GCATGC [GA]CATG^[CT]
1256 GCATGT
SacI 88 GAGCTC GAGCT^C
1270 GAGCTC
StuI 497 AGGCCT AGG^CCT
1058 AGGCCT
StyI 242 CCTTGG C^C[AT][AT]GG
494 CCAAGG
TaqI 623 TCGA T^CGA
825 TCGA
Tsp509I 820 AATT ^AATT
865 AATT
TthHB8I 623 TCGA T^CGA
825 TCGA
XhoII 320 AGATCT [GA]^GATC[CT]
563 AGATCT
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanII BbvI BcnI BglI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp45I TspRI