(Circular) (Six-base) MAPSORT of: pFN21AB4017.seq Check: 6275 from: 1 to: 6097 pFN21AB4017 6097 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3215 3215 Size: 6097 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3440 1937 Size: 1503 4594 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2404 3254 4160 5544 5812 1632 Size: 772 850 906 1384 268 1917 Fragments arranged by size: 1917 1384 906 850 772 268 AfeI AGC'GCT Cuts at: 2585 2585 Size: 6097 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 3090 4996 5973 1203 Size: 1887 1906 977 1327 Fragments arranged by size: 1906 1887 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 3083 1166 Size: 1917 4180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2234 2549 1184 Size: 1050 315 4732 Fragments arranged by size: 4732 1050 315 AlwNI CAG_nnn'CTG Cuts at: 2194 2320 3232 3840 5412 2194 Size: 126 912 608 1572 2879 Fragments arranged by size: 2879 1572 912 608 126 ApaI G_GGCC'C Cuts at: 2090 2090 Size: 6097 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 3406 3632 3784 5893 1493 Size: 1913 226 152 2109 1697 Fragments arranged by size: 2109 1913 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2090 3132 3422 1870 Size: 73 147 1042 290 4545 Fragments arranged by size: 4545 1042 290 147 73 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2106 2635 3418 4873 618 Size: 325 217 627 319 529 783 1455 1842 Fragments arranged by size: 1842 1455 783 627 529 325 319 217 BanII G_rGCy'C Cuts at: 1391 2090 2097 2270 2891 3279 3416 1391 Size: 699 7 173 621 388 137 4072 Fragments arranged by size: 4072 699 621 388 173 137 7 BbeI G_GCGC'C Cuts at: 2110 2110 Size: 6097 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2014 3149 928 Size: 971 115 1135 3876 Fragments arranged by size: 3876 1135 971 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2801 2835 4288 4322 1903 Size: 34 864 34 1453 34 3678 Fragments arranged by size: 3678 1453 864 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 3312 1548 Size: 1764 4333 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2199 2949 3062 Size: 107 122 71 631 605 527 750 113 Cuts at: 3062 3125 4708 136 Size: 63 1583 1525 Fragments arranged by size: 1583 1525 750 631 605 527 122 113 107 71 63 BglII A'GATC_T Cuts at: 2325 2568 6092 2325 Size: 243 3524 2330 Fragments arranged by size: 3524 2330 243 BlpI GC'TnA_GC Cuts at: 2694 3514 2694 Size: 820 5277 Bme1580I G_kGCm'C Cuts at: 1790 2090 2640 4158 5314 1790 Size: 300 550 1518 1156 2573 Fragments arranged by size: 2573 1518 1156 550 300 BmgBI CAC'GTC Cuts at: 1318 3085 3213 1318 Size: 1767 128 4202 Fragments arranged by size: 4202 1767 128 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2108 3828 4786 Size: 416 53 83 399 24 691 1720 958 Cuts at: 4786 442 Size: 1753 Fragments arranged by size: 1753 1720 958 691 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2353 2923 4757 1239 Size: 1114 570 1834 2579 Fragments arranged by size: 2579 1834 1114 570 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2042 4760 882 Size: 617 543 2718 2219 Fragments arranged by size: 2718 2219 617 543 BsaAI yAC'GTr Cuts at: 493 1204 3888 493 Size: 711 2684 2702 Fragments arranged by size: 2702 2684 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2107 3146 3904 4286 Size: 53 83 186 936 574 1039 758 382 Cuts at: 4286 4921 275 Size: 635 1451 Fragments arranged by size: 1451 1039 936 758 635 574 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3048 4530 4928 5202 5349 5760 3048 Size: 1482 398 274 147 411 3385 Fragments arranged by size: 3385 1482 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2300 2330 2363 2393 2990 Size: 30 165 30 897 30 33 30 597 Cuts at: 2990 3020 1178 Size: 30 4255 Fragments arranged by size: 4255 897 597 165 33 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 2530 3014 3017 1186 Size: 1192 152 484 3 4266 Fragments arranged by size: 4266 1192 484 152 3 BseYI C'CCAG_C Cuts at: 2639 2720 3332 5300 5796 2639 Size: 81 612 1968 496 2940 Fragments arranged by size: 2940 1968 612 496 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2992 3193 1163 Size: 573 1256 201 4067 Fragments arranged by size: 4067 1256 573 201 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2209 2632 3547 4308 5336 1540 Size: 275 186 208 423 915 761 1028 2301 Fragments arranged by size: 2301 1028 915 761 423 275 208 186 BsiHKAI G_wGCw'C Cuts at: 2097 3279 4158 4243 5314 2097 Size: 1182 879 85 1071 2880 Fragments arranged by size: 2880 1182 1071 879 85 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2097 2270 2640 2891 3279 3416 Size: 399 300 7 173 370 251 388 137 Cuts at: 3416 4158 4243 5314 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 388 370 300 251 173 137 85 7 BspEI T'CCGG_A Cuts at: 3048 5760 3048 Size: 2712 3385 BspHI T'CATG_A Cuts at: 3986 5716 3986 Size: 1730 4367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2772 3215 3452 844 Size: 1928 443 237 3489 Fragments arranged by size: 3489 1928 443 237 BsrBI CCG'CTC Cuts at: 1394 1823 2058 3984 1394 Size: 429 235 1926 3507 Fragments arranged by size: 3507 1926 429 235 BsrDI GCAATG_nn' Cuts at: 65 1248 2482 4592 4766 65 Size: 1183 1234 2110 174 1396 Fragments arranged by size: 2110 1396 1234 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2392 2703 4741 4928 1700 Size: 110 582 311 2038 187 2869 Fragments arranged by size: 2869 2038 582 311 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 2320 2484 794 Size: 1526 164 4407 Fragments arranged by size: 4407 1526 164 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 2395 2848 1184 Size: 1211 453 4433 Fragments arranged by size: 4433 1211 453 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2758 3068 2011 Size: 747 310 5040 Fragments arranged by size: 5040 747 310 BstYI r'GATC_y Cuts at: 1972 2325 2568 3427 3794 4179 4196 5648 5734 Size: 353 243 859 367 385 17 1452 86 Cuts at: 5734 5746 6092 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 859 385 367 353 346 243 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3020 3020 Size: 6097 BtgI C'CryG_G Cuts at: 513 1065 2098 513 Size: 552 1033 4512 Fragments arranged by size: 4512 1033 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 2195 2321 3613 4407 4427 2195 Size: 126 1292 794 20 3865 Fragments arranged by size: 3865 1292 794 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2462 3367 5104 809 Size: 507 1146 905 1737 1802 Fragments arranged by size: 1802 1737 1146 905 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2223 2808 Size: 54 1425 50 275 18 234 159 585 Cuts at: 2808 3544 4433 6062 8 Size: 736 889 1629 43 Fragments arranged by size: 1629 1425 889 736 585 275 234 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 2669 4027 1353 Size: 683 633 1358 3423 Fragments arranged by size: 3423 1358 683 633 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2095 3277 2095 Size: 1182 4915 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2353 2404 2923 3254 4160 4757 5544 Size: 393 721 51 519 331 906 597 787 Cuts at: 5544 5812 1239 Size: 268 1524 Fragments arranged by size: 1524 906 787 721 597 519 393 331 268 51 EcoNI CCTnn'n_nnAGG Cuts at: 3324 3324 Size: 6097 EcoO109I rG'GnC_Cy Cuts at: 2086 2087 2611 3104 3127 3329 2086 Size: 1 524 493 23 202 4854 Fragments arranged by size: 4854 524 493 202 23 1 EcoRI G'AATT_C Cuts at: 3406 3784 3406 Size: 378 5719 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 2110 2472 2587 2701 5244 1678 Size: 432 362 115 114 2543 2531 Fragments arranged by size: 2543 2531 432 362 115 114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4738 4770 4812 4844 1373 Size: 32 135 32 3166 32 42 32 2626 Fragments arranged by size: 3166 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3441 3687 669 Size: 988 281 1503 246 3079 Fragments arranged by size: 3079 1503 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KasI G'GCGC_C Cuts at: 2106 2106 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 2824 3696 2824 Size: 872 5225 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2181 5211 5395 1305 Size: 876 3030 184 2007 Fragments arranged by size: 3030 2007 876 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2066 2225 10 Size: 54 1425 343 234 159 3882 Fragments arranged by size: 3882 1425 343 234 159 54 MslI CAynn'nnrTG Cuts at: 518 1166 2957 3083 4055 4414 4573 518 Size: 648 1791 126 972 359 159 2042 Fragments arranged by size: 2042 1791 972 648 359 159 126 MspA1I CmG'CkG Cuts at: 1606 2272 2286 2724 2856 2976 2988 3513 4190 Size: 666 14 438 132 120 12 525 677 Cuts at: 4190 5338 5583 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 666 525 438 245 132 120 14 12 NaeI GCC'GGC Cuts at: 2705 2705 Size: 6097 NarI GG'CG_CC Cuts at: 2107 2107 Size: 6097 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5545 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NgoMIV G'CCGG_C Cuts at: 2703 2703 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 2662 3265 3455 5000 1132 Size: 97 1433 603 190 1545 2229 Fragments arranged by size: 2229 1545 1433 603 190 97 PasI CC'CwG_GG Cuts at: 1348 3296 1348 Size: 1948 4149 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 2488 1108 Size: 1380 4717 PfoI T'CCnGG_A Cuts at: 3219 3219 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3194 1613 Size: 1581 4516 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PspOMI G'GGCC_C Cuts at: 2086 2086 Size: 6097 PstI C_TGCA'G Cuts at: 830 2085 2165 2316 2325 3449 830 Size: 1255 80 151 9 1124 3478 Fragments arranged by size: 3478 1255 1124 151 80 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 2724 2856 2976 2724 Size: 132 120 5845 Fragments arranged by size: 5845 132 120 SacI G_AGCT'C Cuts at: 2097 3279 2097 Size: 1182 4915 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SapI GCTCTTCn'nnn_ Cuts at: 2669 2669 Size: 6097 SbfI CC_TGCA'GG Cuts at: 2085 3449 2085 Size: 1364 4733 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 2081 2161 2312 2321 3445 4580 5261 Size: 219 1036 80 151 9 1124 1135 681 Cuts at: 5261 5452 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1124 1036 681 219 191 151 80 9 SfiI GGCCn_nnn'nGGCC Cuts at: 3125 3125 Size: 6097 SfoI GGC'GCC Cuts at: 2108 2108 Size: 6097 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4201 5102 5364 820 Size: 197 926 2258 901 262 1553 Fragments arranged by size: 2258 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 2662 3455 1132 Size: 1530 793 3774 Fragments arranged by size: 3774 1530 793 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 2504 3065 1517 Size: 987 561 4549 Fragments arranged by size: 4549 987 561 StyI C'CwwG_G Cuts at: 513 1065 2247 2499 3393 3536 513 Size: 552 1182 252 894 143 3074 Fragments arranged by size: 3074 1182 894 552 252 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2369 2787 3137 4124 4141 4294 4479 Size: 1255 789 418 350 987 17 153 185 Cuts at: 4479 4924 325 Size: 445 1498 Fragments arranged by size: 1498 1255 987 789 445 418 350 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2646 4343 6055 Size: 274 80 33 51 1443 669 1697 1712 Cuts at: 6055 96 Size: 138 Fragments arranged by size: 1712 1697 1443 669 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2300 2332 2990 3022 6057 6089 2300 Size: 32 658 32 3035 32 2308 Fragments arranged by size: 3035 2308 658 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2123 1316 Size: 807 5290 XbaI T'CTAG_A Cuts at: 2041 3433 2041 Size: 1392 4705 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2953 2953 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI