Restriction map for
pFN21AB4017
(Linear) (Six-base) MAPSORT of: KIBB4017.seq Check: 8748 from: 1 to: 1392
KIBB4017.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1210 1392
Size: 1210 182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 1249 1392
Size: 399 850 143
Fragments arranged by size:
850 399 143
AfeI AGC'GCT
Cuts at: 0 580 1392
Size: 580 812
AflIII A'CryG_T
Cuts at: 0 1085 1392
Size: 1085 307
AleI CACnn'nnGTG
Cuts at: 0 1078 1392
Size: 1078 314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 229 544 1392
Size: 229 315 848
Fragments arranged by size:
848 315 229
AlwNI CAG_nnn'CTG
Cuts at: 0 189 315 1227 1392
Size: 189 126 912 165
Fragments arranged by size:
912 189 165 126
ApaI G_GGCC'C
Cuts at: 0 85 1392
Size: 85 1307
AvaI C'yCGr_G
Cuts at: 0 85 1127 1392
Size: 85 1042 265
Fragments arranged by size:
1042 265 85
BanI G'GyrC_C
Cuts at: 0 101 630 1392
Size: 101 529 762
Fragments arranged by size:
762 529 101
BanII G_rGCy'C
Cuts at: 0 85 92 265 886 1274 1392
Size: 85 7 173 621 388 118
Fragments arranged by size:
621 388 173 118 85 7
BbeI G_GCGC'C
Cuts at: 0 105 1392
Size: 105 1287
BbsI GAAGACnn'nnnn_
Cuts at: 0 9 1144 1392
Size: 9 1135 248
Fragments arranged by size:
1135 248 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 796 830 1392
Size: 796 34 562
Fragments arranged by size:
796 562 34
BclI T'GATC_A
Cuts at: 0 1307 1392
Size: 1307 85
BglI GCCn_nnn'nGGC
Cuts at: 0 194 944 1057 1120 1392
Size: 194 750 113 63 272
Fragments arranged by size:
750 272 194 113 63
BglII A'GATC_T
Cuts at: 0 320 563 1392
Size: 320 243 829
Fragments arranged by size:
829 320 243
BlpI GC'TnA_GC
Cuts at: 0 689 1392
Size: 689 703
Bme1580I G_kGCm'C
Cuts at: 0 85 635 1392
Size: 85 550 757
Fragments arranged by size:
757 550 85
BmgBI CAC'GTC
Cuts at: 0 1080 1208 1392
Size: 1080 128 184
Fragments arranged by size:
1080 184 128
BmrI ACTGGGnnnn_n'
Cuts at: 0 103 1392
Size: 103 1289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 918 1392
Size: 348 570 474
Fragments arranged by size:
570 474 348
BsaI GGTCTCn'nnnn_
Cuts at: 0 37 1392
Size: 37 1355
BsaHI Gr'CG_yC
Cuts at: 0 102 1141 1392
Size: 102 1039 251
Fragments arranged by size:
1039 251 102
BsaWI w'CCGG_w
Cuts at: 0 1043 1392
Size: 1043 349
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 358 388 985 1015 1392
Size: 295 30 33 30 597 30 377
Fragments arranged by size:
597 377 295 33 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 525 1009 1012 1392
Size: 373 152 484 3 380
Fragments arranged by size:
484 380 373 152 3
BseYI C'CCAG_C
Cuts at: 0 634 715 1327 1392
Size: 634 81 612 65
Fragments arranged by size:
634 612 81 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 1188 1392
Size: 987 201 204
Fragments arranged by size:
987 204 201
BsiEI CG_ry'CG
Cuts at: 0 204 627 1392
Size: 204 423 765
Fragments arranged by size:
765 423 204
BsiHKAI G_wGCw'C
Cuts at: 0 92 1274 1392
Size: 92 1182 118
Fragments arranged by size:
1182 118 92
Bsp1286I G_dGCh'C
Cuts at: 0 85 92 265 635 886 1274 1392
Size: 85 7 173 370 251 388 118
Fragments arranged by size:
388 370 251 173 118 85 7
BspEI T'CCGG_A
Cuts at: 0 1043 1392
Size: 1043 349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 767 1210 1392
Size: 767 443 182
Fragments arranged by size:
767 443 182
BsrBI CCG'CTC
Cuts at: 0 53 1392
Size: 53 1339
BsrDI GCAATG_nn'
Cuts at: 0 477 1392
Size: 477 915
BsrFI r'CCGG_y
Cuts at: 0 387 698 1392
Size: 387 311 694
Fragments arranged by size:
694 387 311
BstAPI GCAn_nnn'nTGC
Cuts at: 0 315 479 1392
Size: 315 164 913
Fragments arranged by size:
913 315 164
BstEII G'GTnAC_C
Cuts at: 0 390 843 1392
Size: 390 453 549
Fragments arranged by size:
549 453 390
BstXI CCAn_nnnn'nTGG
Cuts at: 0 753 1063 1392
Size: 753 310 329
Fragments arranged by size:
753 329 310
BstYI r'GATC_y
Cuts at: 0 320 563 1392
Size: 320 243 829
Fragments arranged by size:
829 320 243
Bsu36I CC'TnA_GG
Cuts at: 0 1015 1392
Size: 1015 377
BtgI C'CryG_G
Cuts at: 0 93 1392
Size: 93 1299
BtsI GCAGTG_nn'
Cuts at: 0 190 316 1392
Size: 190 126 1076
Fragments arranged by size:
1076 190 126
DrdI GACnn_nn'nnGTC
Cuts at: 0 457 1362 1392
Size: 457 905 30
Fragments arranged by size:
905 457 30
EaeI y'GGCC_r
Cuts at: 0 59 218 803 1392
Size: 59 159 585 589
Fragments arranged by size:
589 585 159 59
EarI CTCTTCn'nnn_
Cuts at: 0 31 664 1392
Size: 31 633 728
Fragments arranged by size:
728 633 31
EcoICRI GAG'CTC
Cuts at: 0 90 1272 1392
Size: 90 1182 120
Fragments arranged by size:
1182 120 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 399 918 1249 1392
Size: 348 51 519 331 143
Fragments arranged by size:
519 348 331 143 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1319 1392
Size: 1319 73
EcoO109I rG'GnC_Cy
Cuts at: 0 81 82 606 1099 1122 1324 1392
Size: 81 1 524 493 23 202 68
Fragments arranged by size:
524 493 202 81 68 23 1
HaeII r_GCGC'y
Cuts at: 0 105 467 582 696 1392
Size: 105 362 115 114 696
Fragments arranged by size:
696 362 115 114 105
KasI G'GCGC_C
Cuts at: 0 101 1392
Size: 101 1291
MfeI C'AATT_G
Cuts at: 0 819 1392
Size: 819 573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1392
Size: 176 1216
MscI TGG'CCA
Cuts at: 0 61 220 1392
Size: 61 159 1172
Fragments arranged by size:
1172 159 61
MslI CAynn'nnrTG
Cuts at: 0 952 1078 1392
Size: 952 126 314
Fragments arranged by size:
952 314 126
MspA1I CmG'CkG
Cuts at: 0 267 281 719 851 971 983 1392
Size: 267 14 438 132 120 12 409
Fragments arranged by size:
438 409 267 132 120 14 12
NaeI GCC'GGC
Cuts at: 0 700 1392
Size: 700 692
NarI GG'CG_CC
Cuts at: 0 102 1392
Size: 102 1290
NgoMIV G'CCGG_C
Cuts at: 0 698 1392
Size: 698 694
NspI r_CATG'y
Cuts at: 0 657 1260 1392
Size: 657 603 132
Fragments arranged by size:
657 603 132
PasI CC'CwG_GG
Cuts at: 0 1291 1392
Size: 1291 101
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 1392
Size: 483 909
PfoI T'CCnGG_A
Cuts at: 0 1214 1392
Size: 1214 178
PshAI GACnn'nnGTC
Cuts at: 0 1189 1392
Size: 1189 203
PspOMI G'GGCC_C
Cuts at: 0 81 1392
Size: 81 1311
PstI C_TGCA'G
Cuts at: 0 80 160 311 320 1392
Size: 80 80 151 9 1072
Fragments arranged by size:
1072 151 80 80 9
PvuII CAG'CTG
Cuts at: 0 719 851 971 1392
Size: 719 132 120 421
Fragments arranged by size:
719 421 132 120
SacI G_AGCT'C
Cuts at: 0 92 1274 1392
Size: 92 1182 118
Fragments arranged by size:
1182 118 92
SapI GCTCTTCn'nnn_
Cuts at: 0 664 1392
Size: 664 728
SbfI CC_TGCA'GG
Cuts at: 0 80 1392
Size: 80 1312
SfcI C'TryA_G
Cuts at: 0 76 156 307 316 1392
Size: 76 80 151 9 1076
Fragments arranged by size:
1076 151 80 76 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1120 1392
Size: 1120 272
SfoI GGC'GCC
Cuts at: 0 103 1392
Size: 103 1289
SphI G_CATG'C
Cuts at: 0 657 1392
Size: 657 735
StuI AGG'CCT
Cuts at: 0 499 1060 1392
Size: 499 561 332
Fragments arranged by size:
561 499 332
StyI C'CwwG_G
Cuts at: 0 242 494 1392
Size: 242 252 898
Fragments arranged by size:
898 252 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 364 782 1132 1392
Size: 364 418 350 260
Fragments arranged by size:
418 364 350 260
TatI w'GTAC_w
Cuts at: 0 641 1392
Size: 641 751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 295 327 985 1017 1392
Size: 295 32 658 32 375
Fragments arranged by size:
658 375 295 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 118 1392
Size: 118 1274
XbaI T'CTAG_A
Cuts at: 0 36 1392
Size: 36 1356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 948 1392
Size: 948 444
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI
PfoI PshAI PspOMI PstI PvuII SacI SapI SbfI
SfcI SfiI SfoI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BfrBI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsiWI BsmI BsmBI BspHI BsrGI BssHII BssSI
BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MluI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PpuMI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI SwaI XhoI XmaI XmnI ZraI