Restriction map for
pFN21AB3998
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaJI BseRI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MscI
MunI MvaI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 537 TCCGGA T^CCGGA
AciI 205 CCGC CCGC(-3/-1)
AcsI 79 AAATTT [GA]^AATT[CT]
AfaI 97 GTAC GT^AC
AflIII 49 ACATGT A^C[GA][CT]GT
AlwNI 416 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 79 AAATTT [GA]^AATT[CT]
AvaI 529 CTCGAG C^[CT]CG[GA]G
BanI 291 GGTGCC G^G[CT][GA]CC
BbvI 523 GCTGC GCAGC(8/12)
BpmI 413 CTCCAG CTGGAG(16/14)
BsaI 252 GGTCTC GGTCTC(1/5)
BsaWI 537 TCCGGA [AT]^CCGG[AT]
BseAI 537 TCCGGA T^CCGGA
BsgI 473 CTGCAC GTGCAG(16/14)
BsiEI 202 CGACCG CG[GA][CT]^CG
BsmFI 340 GGGAC GGGAC(10/14)
BsoBI 529 CTCGAG C^[CT]CG[GA]G
BspEI 537 TCCGGA T^CCGGA
BspLU11I 49 ACATGT A^CATGT
Cac8I 432 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 435 GGCCC G^G[ACGT]CC
DpnII 352 GATC ^GATC
EcoRV 60 GATATC GAT^ATC
Fnu4HI 523 GCTGC GC^[ACGT]GC
HaeIII 435 GGCC GG^CC
HapII 538 CCGG C^CGG
HpaII 538 CCGG C^CGG
HphI 306 TCACC GGTGA(8/7)
ItaI 523 GCTGC GC^[ACGT]GC
MaeII 275 ACGT A^CGT
MboI 352 GATC ^GATC
MroI 537 TCCGGA T^CCGGA
MseI 11 TTAA T^TAA
MslI 126 CACAGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 205 CCGCTG C[AC]G^C[GT]G
MspI 538 CCGG C^CGG
MwoI 514 GCTATCCTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 352 GATC ^GATC
NlaIII 50 CATG CATG^
NlaIV 291 GGTGCC GG[ACGT]^[ACGT]CC
NspI 49 ACATGT [GA]CATG^[CT]
PaeR7I 529 CTCGAG C^TCGAG
PstI 173 CTGCAG CTGCA^G
RsaI 97 GTAC GT^AC
Sau3AI 352 GATC ^GATC
Sau96I 435 GGCCC G^G[ACGT]CC
SfcI 173 CTGCAG C^T[GA][CT]AG
SmlI 529 CTCGAG C^T[CT][GA]AG
TaqI 530 TCGA T^CGA
Tru9I 11 TTAA T^TAA
TseI 523 GCTGC G^C[AT]GC
TthHB8I 530 TCGA T^CGA
XhoI 529 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 9 enzymes) #
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AluI 431 AGCT AG^CT
522 AGCT
BfaI 235 CTAG C^TAG
520 CTAG
HinfI 321 GACTC G^A[ACGT]TC
342 GACTC
MaeI 235 CTAG C^TAG
520 CTAG
MaeIII 124 GTCAC ^GT[ACGT]AC
440 GTGAC
MboII 495 GAAGA GAAGA(8/7)
262 TCTTC
PleI 321 GACTC GAGTC(4/5)
342 GACTC
Tsp45I 124 GTCAC ^GT[GC]AC
440 GTGAC
TspRI 248 CAGTG CA[GC]TG[ACGT][ACGT]^
310 CACTG
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
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BsmAI DdeI MnlI Tsp509I