(Circular) (Six-base) MAPSORT of: pFN21AB3968.seq Check: 5829 from: 1 to: 6040 pFN21AB3968 6040 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2352 3189 3383 1937 Size: 415 837 194 4594 Fragments arranged by size: 4594 837 415 194 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4103 5487 5755 1632 Size: 2471 1384 268 1917 Fragments arranged by size: 2471 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 2589 4939 5916 1203 Size: 1386 2350 977 1327 Fragments arranged by size: 2350 1386 1327 977 AgeI A'CCGG_T Cuts at: 2502 4871 2502 Size: 2369 3671 AhdI GACnn_n'nnGTC Cuts at: 2062 4769 2062 Size: 2707 3333 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 2144 3166 3244 3783 5355 2144 Size: 1022 78 539 1572 2829 Fragments arranged by size: 2829 1572 1022 539 78 ApaI G_GGCC'C Cuts at: 2179 2179 Size: 6040 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 3349 3575 3727 5836 1493 Size: 1856 226 152 2109 1697 Fragments arranged by size: 2109 1856 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 2051 3365 1870 Size: 73 108 1314 4545 Fragments arranged by size: 4545 1314 108 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3361 4816 618 Size: 325 217 627 1574 1455 1842 Fragments arranged by size: 1842 1574 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2179 2922 3359 1391 Size: 788 743 437 4072 Fragments arranged by size: 4072 788 743 437 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2184 2783 928 Size: 971 285 599 4185 Fragments arranged by size: 4185 971 599 285 BbvCI CC'TCA_GC Cuts at: 2015 2015 Size: 6040 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 1548 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3135 4651 136 Size: 107 122 71 631 605 1463 1516 1525 Fragments arranged by size: 1525 1516 1463 631 605 122 107 71 BglII A'GATC_T Cuts at: 6035 6035 Size: 6040 BlpI GC'TnA_GC Cuts at: 2387 3457 2387 Size: 1070 4970 Bme1580I G_kGCm'C Cuts at: 1790 2179 4101 5257 1790 Size: 389 1922 1156 2573 Fragments arranged by size: 2573 1922 1156 389 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3771 4729 442 Size: 416 53 83 399 24 2354 958 1753 Fragments arranged by size: 2354 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4700 1239 Size: 3461 2579 Bpu10I CC'TnA_GC Cuts at: 2015 2015 Size: 6040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4165 5030 5328 4165 Size: 865 298 4877 Fragments arranged by size: 4877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3297 4703 882 Size: 617 1798 1406 2219 Fragments arranged by size: 2219 1798 1406 617 BsaAI yAC'GTr Cuts at: 493 1204 3831 493 Size: 711 2627 2702 Fragments arranged by size: 2702 2627 711 BsaBI GATnn'nnATC Cuts at: 2291 6034 2291 Size: 3743 2297 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3847 4229 4864 275 Size: 53 83 186 936 2314 382 635 1451 Fragments arranged by size: 2314 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2502 2994 4473 4871 5145 5292 5703 2502 Size: 492 1479 398 274 147 411 2839 Fragments arranged by size: 2839 1479 492 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2054 2084 1178 Size: 30 165 30 651 30 5134 Fragments arranged by size: 5134 651 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6040 BseYI C'CCAG_C Cuts at: 5243 5739 5243 Size: 496 5544 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5467 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3490 4251 5279 1540 Size: 275 186 1489 761 1028 2301 Fragments arranged by size: 2301 1489 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4101 4186 5257 4101 Size: 85 1071 4884 Fragments arranged by size: 4884 1071 85 BsmI GAATG_Cn' Cuts at: 3551 3644 3551 Size: 93 5947 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2179 2922 3359 4101 4186 5257 1391 Size: 399 389 743 437 742 85 1071 2174 Fragments arranged by size: 2174 1071 743 742 437 399 389 85 BspEI T'CCGG_A Cuts at: 2994 5703 2994 Size: 2709 3331 BspHI T'CATG_A Cuts at: 3929 5659 3929 Size: 1730 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3395 844 Size: 2551 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3927 1394 Size: 429 2104 3507 Fragments arranged by size: 3507 2104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2612 4535 4709 65 Size: 1183 1364 1923 174 1396 Fragments arranged by size: 1923 1396 1364 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 2502 4684 4871 1700 Size: 110 228 464 2182 187 2869 Fragments arranged by size: 2869 2182 464 228 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 4100 5112 4100 Size: 1012 5028 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6040 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstYI r'GATC_y Cuts at: 1972 3370 3737 4122 4139 5591 5677 5689 6035 Size: 1398 367 385 17 1452 86 12 346 Cuts at: 6035 1972 Size: 1977 Fragments arranged by size: 1977 1452 1398 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3190 3190 Size: 6040 BtgI C'CryG_G Cuts at: 513 1065 3130 513 Size: 552 2065 3423 Fragments arranged by size: 3423 2065 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3007 3178 487 Size: 683 56 1781 171 3349 Fragments arranged by size: 3349 1781 683 171 56 BtsI GCAGTG_nn' Cuts at: 3556 4350 4370 3556 Size: 794 20 5226 Fragments arranged by size: 5226 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 3097 3344 3097 Size: 247 5793 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2235 3127 3171 Size: 54 1425 50 275 18 405 892 44 Cuts at: 3171 3254 3487 4376 6005 8 Size: 83 233 889 1629 43 Fragments arranged by size: 1629 1425 892 889 405 275 233 83 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3487 1537 Size: 275 1675 4090 Fragments arranged by size: 4090 1675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2466 3970 1353 Size: 1113 1504 3423 Fragments arranged by size: 3423 1504 1113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4627 5001 5147 387 Size: 1064 138 3038 374 146 1280 Fragments arranged by size: 3038 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4103 4700 5487 5755 1239 Size: 393 2471 597 787 268 1524 Fragments arranged by size: 2471 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2377 2377 Size: 6040 EcoO109I rG'GnC_Cy Cuts at: 2164 2175 2176 3212 2164 Size: 11 1 1036 4992 Fragments arranged by size: 4992 1036 11 1 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 5187 1678 Size: 3509 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2054 2080 2084 2086 Size: 32 135 32 476 6 26 4 2 Cuts at: 2086 2116 2850 2882 4681 4713 4755 4787 1373 Size: 30 734 32 1799 32 42 32 2626 Fragments arranged by size: 2626 1799 734 476 135 42 32 32 32 32 32 30 26 6 4 2 HincII GTy'rAC Cuts at: 669 1657 1938 3384 3630 669 Size: 988 281 1446 246 3079 Fragments arranged by size: 3079 1446 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 2726 5154 5338 1305 Size: 903 518 2428 184 2007 Fragments arranged by size: 2428 2007 903 518 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2237 3129 3173 10 Size: 54 1425 343 405 892 44 2877 Fragments arranged by size: 2877 1425 892 405 343 54 44 MslI CAynn'nnrTG Cuts at: 518 1166 2361 3998 4357 4516 518 Size: 648 1195 1637 359 159 2042 Fragments arranged by size: 2042 1637 1195 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2391 3456 4133 5281 5526 1606 Size: 785 1065 677 1148 245 2120 Fragments arranged by size: 2120 1148 1065 785 677 245 NcoI C'CATG_G Cuts at: 513 1065 3130 513 Size: 552 2065 3423 Fragments arranged by size: 3423 2065 552 NdeI CA'TA_TG Cuts at: 387 2362 387 Size: 1975 4065 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 2545 2593 3398 4943 1132 Size: 97 1316 48 805 1545 2229 Fragments arranged by size: 2229 1545 1316 805 97 48 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 2589 4939 2589 Size: 2350 3690 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6040 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2164 2164 Size: 6040 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PspOMI G'GGCC_C Cuts at: 2175 2175 Size: 6040 PstI C_TGCA'G Cuts at: 830 2149 2583 2943 3392 830 Size: 1319 434 360 449 3478 Fragments arranged by size: 3478 1319 449 434 360 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 2391 2391 Size: 6040 RsrII CG'GwC_CG Cuts at: 2992 2992 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SapI GCTCTTCn'nnn_ Cuts at: 2466 2466 Size: 6040 SbfI CC_TGCA'GG Cuts at: 2943 3392 2943 Size: 449 5591 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 2145 2579 2939 3388 4523 5204 5395 Size: 219 1100 434 360 449 1135 681 191 Cuts at: 5395 826 Size: 1471 Fragments arranged by size: 1471 1135 1100 681 449 434 360 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4144 5045 5307 820 Size: 197 926 2201 901 262 1553 Fragments arranged by size: 2201 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6040 StyI C'CwwG_G Cuts at: 513 1065 3130 3479 513 Size: 552 2065 349 3074 Fragments arranged by size: 3074 2065 552 349 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2203 4067 4084 4237 4422 4867 325 Size: 1255 623 1864 17 153 185 445 1498 Fragments arranged by size: 1864 1498 1255 623 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2548 2880 2946 Size: 274 80 33 51 1443 571 332 66 Cuts at: 2946 4286 5998 96 Size: 1340 1712 138 Fragments arranged by size: 1712 1443 1340 571 332 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2975 2975 Size: 6040 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 4169 4169 Size: 6040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI