(Circular) (Six-base) MAPSORT of: pFN21AB3919.seq Check: 7940 from: 1 to: 6442 pFN21AB3919 6442 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2875 4252 278 Size: 53 83 186 936 1339 1377 2468 Fragments arranged by size: 2468 1377 1339 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3785 1937 Size: 1848 4594 Acc65I G'GTAC_C Cuts at: 3763 3763 Size: 6442 AclI AA'CG_TT Cuts at: 1563 4569 4942 1563 Size: 3006 373 3063 Fragments arranged by size: 3063 3006 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 2775 3126 3159 3527 4505 5889 6157 Size: 605 538 351 33 368 978 1384 268 Cuts at: 6157 1632 Size: 1917 Fragments arranged by size: 1917 1384 978 605 538 368 351 268 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6245 AflIII A'CryG_T Cuts at: 1203 2121 3008 5341 6318 1203 Size: 918 887 2333 977 1327 Fragments arranged by size: 2333 1327 977 918 887 AgeI A'CCGG_T Cuts at: 5273 5273 Size: 6442 AhdI GACnn_n'nnGTC Cuts at: 5171 5171 Size: 6442 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6442 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6442 AlwNI CAG_nnn'CTG Cuts at: 4185 5757 4185 Size: 1572 4870 ApaI G_GGCC'C Cuts at: 2155 3171 2155 Size: 1016 5426 ApaLI G'TGCA_C Cuts at: 4499 5655 4499 Size: 1156 5286 ApoI r'AATT_y Cuts at: 1493 2440 2819 3411 3547 3660 3669 3751 3977 Size: 947 379 592 136 113 9 82 226 Cuts at: 3977 4129 6238 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 947 592 379 226 152 136 113 82 9 AseI AT'TA_AT Cuts at: 160 1032 4996 160 Size: 872 3964 1606 Fragments arranged by size: 3964 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 2508 3767 1870 Size: 73 565 1259 4545 Fragments arranged by size: 4545 1259 565 73 BamHI G'GATC_C Cuts at: 2561 3772 4139 2561 Size: 1211 367 4864 Fragments arranged by size: 4864 1211 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2648 2759 3257 3763 5218 Size: 325 217 627 861 111 498 506 1455 Cuts at: 5218 618 Size: 1842 Fragments arranged by size: 1842 1455 861 627 506 498 325 217 111 BanII G_rGCy'C Cuts at: 1391 2155 2540 3171 3761 1391 Size: 764 385 631 590 4072 Fragments arranged by size: 4072 764 631 590 385 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2117 928 Size: 971 218 5253 Fragments arranged by size: 5253 971 218 BbvCI CC'TCA_GC Cuts at: 2030 3021 2030 Size: 991 5451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2225 2259 3281 3315 4633 4667 1903 Size: 34 288 34 1022 34 1318 34 3678 Fragments arranged by size: 3678 1318 1022 288 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2578 4334 5544 2578 Size: 1756 1210 3476 Fragments arranged by size: 3476 1756 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6442 BfrBI ATG'CAT Cuts at: 2797 2797 Size: 6442 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2159 2168 5053 Size: 107 122 71 631 605 487 9 2885 Cuts at: 5053 136 Size: 1525 Fragments arranged by size: 2885 1525 631 605 487 122 107 71 9 BglII A'GATC_T Cuts at: 6437 6437 Size: 6442 BlpI GC'TnA_GC Cuts at: 3859 3859 Size: 6442 Bme1580I G_kGCm'C Cuts at: 1790 2155 3171 4503 5659 1790 Size: 365 1016 1332 1156 2573 Fragments arranged by size: 2573 1332 1156 1016 365 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6442 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2674 4173 5131 Size: 416 53 83 399 24 1257 1499 958 Cuts at: 5131 442 Size: 1753 Fragments arranged by size: 1753 1499 1257 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2541 2807 3315 3330 5102 1239 Size: 1302 266 508 15 1772 2579 Fragments arranged by size: 2579 1772 1302 508 266 15 Bpu10I CC'TnA_GC Cuts at: 2030 3021 3402 2030 Size: 991 381 5070 Fragments arranged by size: 5070 991 381 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2141 2527 2793 4567 5432 5730 2141 Size: 386 266 1774 865 298 2853 Fragments arranged by size: 2853 1774 865 386 298 266 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2559 5105 882 Size: 617 1060 2546 2219 Fragments arranged by size: 2546 2219 1060 617 BsaAI yAC'GTr Cuts at: 493 1204 4233 493 Size: 711 3029 2702 Fragments arranged by size: 3029 2702 711 BsaBI GATnn'nnATC Cuts at: 2566 2738 6436 2566 Size: 172 3698 2572 Fragments arranged by size: 3698 2572 172 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 2872 4249 4631 Size: 53 83 186 936 543 796 1377 382 Cuts at: 4631 5266 275 Size: 635 1451 Fragments arranged by size: 1451 1377 936 796 635 543 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4875 5273 5547 5694 6105 4875 Size: 398 274 147 411 5212 Fragments arranged by size: 5212 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2926 2956 1178 Size: 30 165 30 1523 30 4664 Fragments arranged by size: 4664 1523 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2530 3038 3041 3177 1186 Size: 1344 508 3 136 4451 Fragments arranged by size: 4451 1344 508 136 3 BseYI C'CCAG_C Cuts at: 2148 2484 3241 5645 6141 2148 Size: 336 757 2404 496 2449 Fragments arranged by size: 2449 2404 757 496 336 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 3579 1163 Size: 573 441 1402 4026 Fragments arranged by size: 4026 1402 573 441 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3892 4653 5681 1540 Size: 275 186 1891 761 1028 2301 Fragments arranged by size: 2301 1891 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 4503 4588 5659 2220 Size: 2283 85 1071 3003 Fragments arranged by size: 3003 2283 1071 85 BsmI GAATG_Cn' Cuts at: 3953 4046 3953 Size: 93 6349 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2155 2220 2540 3171 3761 4503 4588 Size: 399 365 65 320 631 590 742 85 Cuts at: 4588 5659 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 631 590 399 365 320 85 65 BspEI T'CCGG_A Cuts at: 6105 6105 Size: 6442 BspHI T'CATG_A Cuts at: 3033 4331 6061 3033 Size: 1298 1730 3414 Fragments arranged by size: 3414 1730 1298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3797 844 Size: 2953 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4329 1394 Size: 429 2506 3507 Fragments arranged by size: 3507 2506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2945 3004 4937 5111 65 Size: 1183 1697 59 1933 174 1396 Fragments arranged by size: 1933 1697 1396 1183 174 59 BsrFI r'CCGG_y Cuts at: 1700 1810 5086 5273 1700 Size: 110 3276 187 2869 Fragments arranged by size: 3276 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6442 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6442 BssSI C'ACGA_G Cuts at: 3576 4502 5514 3576 Size: 926 1012 4504 Fragments arranged by size: 4504 1012 926 BstAPI GCAn_nnn'nTGC Cuts at: 794 3347 794 Size: 2553 3889 BstBI TT'CG_AA Cuts at: 5246 5246 Size: 6442 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6442 BstXI CCAn_nnnn'nTGG Cuts at: 2624 2624 Size: 6442 BstYI r'GATC_y Cuts at: 1972 2082 2561 2832 3772 4139 4524 4541 5993 Size: 110 479 271 940 367 385 17 1452 Cuts at: 5993 6079 6091 6437 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 940 479 385 367 346 271 110 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3163 3163 Size: 6442 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5890 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5703 Fragments arranged by size: 5703 683 56 BtsI GCAGTG_nn' Cuts at: 3958 4752 4772 3958 Size: 794 20 5628 Fragments arranged by size: 5628 794 20 ClaI AT'CG_AT Cuts at: 1554 2691 1554 Size: 1137 5305 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2602 2637 529 Size: 35 2038 35 4334 Fragments arranged by size: 4334 2038 35 35 DraI TTT'AAA Cuts at: 3746 3746 Size: 6442 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5449 809 Size: 507 4133 1802 Fragments arranged by size: 4133 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2478 3889 4778 Size: 54 1425 50 275 18 648 1411 889 Cuts at: 4778 6407 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1411 889 648 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3889 1537 Size: 275 2077 4090 Fragments arranged by size: 4090 2077 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2823 3450 4372 1353 Size: 1470 627 922 3423 Fragments arranged by size: 3423 1470 922 627 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5029 5403 5549 387 Size: 1064 138 3440 374 146 1280 Fragments arranged by size: 3440 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2541 2775 2807 3126 3159 3315 Size: 393 605 304 234 32 319 33 156 Cuts at: 3315 3330 3527 4505 5102 5889 6157 1239 Size: 15 197 978 597 787 268 1524 Fragments arranged by size: 1524 978 787 605 597 393 319 304 268 234 197 156 33 32 15 EcoO109I rG'GnC_Cy Cuts at: 2096 2152 3167 3168 2096 Size: 56 1015 1 5370 Fragments arranged by size: 5370 1015 56 1 EcoRI G'AATT_C Cuts at: 3751 4129 3751 Size: 378 6064 EcoRV GAT'ATC Cuts at: 2940 2940 Size: 6442 FspI TGC'GCA Cuts at: 4948 4948 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 2174 5589 1678 Size: 496 3415 2531 Fragments arranged by size: 3415 2531 496 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2836 2868 3139 3171 3214 Size: 32 135 32 1264 32 271 32 43 Cuts at: 3214 3246 5083 5115 5157 5189 1373 Size: 32 1837 32 42 32 2626 Fragments arranged by size: 2626 1837 1264 271 135 43 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3786 4032 669 Size: 988 281 1848 246 3079 Fragments arranged by size: 3079 1848 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6442 HpaI GTT'AAC Cuts at: 4032 4032 Size: 6442 KpnI G_GTAC'C Cuts at: 3767 3767 Size: 6442 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 2121 6318 2121 Size: 4197 2245 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2605 2848 5556 5740 1305 Size: 1300 243 2708 184 2007 Fragments arranged by size: 2708 2007 1300 243 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4620 Fragments arranged by size: 4620 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3633 4400 4759 4918 518 Size: 648 2467 767 359 159 2042 Fragments arranged by size: 2467 2042 767 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3335 3858 4535 5683 5928 1606 Size: 1729 523 677 1148 245 2120 Fragments arranged by size: 2120 1729 1148 677 523 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5890 NdeI CA'TA_TG Cuts at: 387 387 Size: 6442 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6442 NotI GC'GGCC_GC Cuts at: 3889 3889 Size: 6442 NsiI A_TGCA'T Cuts at: 2799 2799 Size: 6442 NspI r_CATG'y Cuts at: 1132 1229 3012 3800 5345 1132 Size: 97 1783 788 1545 2229 Fragments arranged by size: 2229 1783 1545 788 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6442 PciI A'CATG_T Cuts at: 3008 5341 3008 Size: 2333 4109 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6442 PfoI T'CCnGG_A Cuts at: 2557 3100 2557 Size: 543 5899 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2336 2368 3377 3409 4511 4543 6024 6056 2336 Size: 32 1009 32 1102 32 1481 32 2722 Fragments arranged by size: 2722 1481 1102 1009 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2096 2096 Size: 6442 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6442 PsiI TTA'TAA Cuts at: 4012 4012 Size: 6442 PspOMI G'GGCC_C Cuts at: 2151 3167 2151 Size: 1016 5426 PstI C_TGCA'G Cuts at: 830 2365 3794 830 Size: 1535 1429 3478 Fragments arranged by size: 3478 1535 1429 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 SalI G'TCGA_C Cuts at: 1936 3784 1936 Size: 1848 4594 SanDI GG'GwC_CC Cuts at: 2096 2096 Size: 6442 SbfI CC_TGCA'GG Cuts at: 3794 3794 Size: 6442 ScaI AGT'ACT Cuts at: 4690 6402 4690 Size: 1712 4730 SfcI C'TryA_G Cuts at: 826 1045 2361 3790 4925 5606 5797 826 Size: 219 1316 1429 1135 681 191 1471 Fragments arranged by size: 1471 1429 1316 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2159 2159 Size: 6442 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6442 SmaI CCC'GGG Cuts at: 1872 3769 1872 Size: 1897 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2156 2542 2808 4546 5447 5709 Size: 197 926 213 386 266 1738 901 262 Cuts at: 5709 820 Size: 1553 Fragments arranged by size: 1738 1553 926 901 386 266 262 213 197 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 2305 152 Size: 2153 4289 SphI G_CATG'C Cuts at: 1132 3800 1132 Size: 2668 3774 SspI AAT'ATT Cuts at: 5 52 4366 5 Size: 47 4314 2081 Fragments arranged by size: 4314 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6442 StyI C'CwwG_G Cuts at: 513 1065 3120 3881 513 Size: 552 2055 761 3074 Fragments arranged by size: 3074 2055 761 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2212 2630 3151 4469 4486 4639 4824 Size: 1255 632 418 521 1318 17 153 185 Cuts at: 4824 5269 325 Size: 445 1498 Fragments arranged by size: 1498 1318 1255 632 521 445 418 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4688 6400 96 Size: 274 80 33 51 1443 2711 1712 138 Fragments arranged by size: 2711 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2926 2958 6402 6434 2926 Size: 32 3444 32 2934 Fragments arranged by size: 3444 2934 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2680 1316 Size: 1364 5078 XbaI T'CTAG_A Cuts at: 3778 3778 Size: 6442 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6442 XmaI C'CCGG_G Cuts at: 1870 3767 1870 Size: 1897 4545 XmnI GAAnn'nnTTC Cuts at: 4571 4571 Size: 6442 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2873 4250 276 Size: 53 83 186 936 1339 1377 2468 Fragments arranged by size: 2468 1377 1339 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfoI SrfI SwaI XcmI