Restriction map for
pFN21AB3906
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BpmI
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 8 CGCG CG^CG
AcsI 544 GAATTT [GA]^AATT[CT]
ApoI 544 GAATTT [GA]^AATT[CT]
AseI 1084 ATTAAT AT^TAAT
AsnI 1084 ATTAAT AT^TAAT
AspHI 903 GAGCAC G[AT]GC[AT]^C
AvaI 53 CCCGGG C^[CT]CG[GA]G
BlpI 397 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 397 GCTCAGC GC^T[ACGT]AGC
BsaAI 628 TACGTG [CT]AC^GT[GA]
BsiHKAI 903 GAGCAC G[AT]GC[AT]^C
BsiWI 215 CGTACG C^GTACG
BsoBI 53 CCCGGG C^[CT]CG[GA]G
Bsp1407I 440 TGTACA T^GTACA
BspMI 161 ACCTGC ACCTGC(4/8)
BsrDI 637 GCAATG GCAATG(2/0)
BsrGI 440 TGTACA T^GTACA
BstUI 8 CGCG CG^CG
CelII 397 GCTCAGC GC^T[ACGT]AGC
DpnII 792 GATC ^GATC
DraIII 121 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 317 GACTTGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 142 GATATC GAT^ATC
EcoT22I 1 ATGCAT ATGCA^T
FauI 183 CCCGC CCCGC(4/6)
HphI 126 GGTGA GGTGA(8/7)
KspI 7 CCGCGG CCGC^GG
MboI 792 GATC ^GATC
MslI 747 CATGGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 8 CGCG CG^CG
NdeII 792 GATC ^GATC
NsiI 1 ATGCAT ATGCA^T
NspI 1194 GCATGC [GA]CATG^[CT]
PshBI 1084 ATTAAT AT^TAAT
SacII 7 CCGCGG CCGC^GG
SapI 177 GAAGAGC GCTCTTC(1/4)
Sau3AI 792 GATC ^GATC
SmaI 53 CCCGGG CCC^GGG
SphI 1194 GCATGC GCATG^C
SspBI 440 TGTACA T^GTACA
SspI 433 AATATT AAT^ATT
TaqI 690 TCGA T^CGA
TthHB8I 690 TCGA T^CGA
XmaCI 53 CCCGGG C^CCGGG
XmaI 53 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 11 GGCGCC G[GA]^CG[CT]C
76 GGCGCC
Asp700I 601 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1072 GAAGTGCTTC
BanII 180 GAGCCC G[GA]GC[CT]^C
470 GAGCCC
BbeI 11 GGCGCC GGCGC^C
76 GGCGCC
BsaHI 11 GGCGCC G[GA]^CG[CT]C
76 GGCGCC
BsmAI 1109 GAGAC GTCTC(1/5)
326 GTCTC
BsmFI 56 GGGAC GGGAC(10/14)
462 GGGAC
BstNI 114 CCTGG CC^[AT]GG
507 CCAGG
CfoI 12 GCGC GCG^C
77 GCGC
DsaI 7 CCGCGG C^C[GA][CT]GG
227 CCACGG
EarI 177 GAAGAG CTCTTC(1/4)
734 GAAGAG
EcoRII 114 CCTGG ^CC[AT]GG
507 CCAGG
EcoT14I 559 CCAAGG C^C[AT][AT]GG
1062 CCTTGG
HaeII 11 GGCGCC [GA]GCGC^[CT]
76 GGCGCC
HaeIII 118 GGCC GG^CC
223 GGCC
HhaI 12 GCGC GCG^C
77 GCGC
Hin1I 11 GGCGCC G[GA]^CG[CT]C
76 GGCGCC
HinP1I 12 GCGC G^CGC
77 GCGC
KasI 11 GGCGCC G^GCGCC
76 GGCGCC
Ksp632I 177 GAAGAG CTCTTC(1/4)
734 GAAGAG
MaeII 629 ACGT A^CGT
983 ACGT
MaeIII 127 GTGAC ^GT[ACGT]AC
514 GTCAC
MvaI 114 CCTGG CC^[AT]GG
507 CCAGG
NarI 11 GGCGCC GG^CGCC
76 GGCGCC
PpuMI 91 AGGTCCT [GA]G^G[AT]CC[CT]
462 GGGACCT
PvuII 286 CAGCTG CAG^CTG
1199 CAGCTG
SfaNI 931 GATGC GCATC(5/9)
3 GCATC
SfoI 11 GGCGCC GGC^GCC
76 GGCGCC
StyI 559 CCAAGG C^C[AT][AT]GG
1062 CCTTGG
Tsp45I 127 GTGAC ^GT[GC]AC
514 GTCAC
XmnI 601 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1072 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BcnI BfaI BmyI BsaJI
BsgI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I Cac8I Cfr13I DdeI
DraII EcoO109I Fnu4HI FokI HapII
HinfI HpaII ItaI MaeI MboII
MnlI MseI MspA1I MspI MwoI
NciI NlaIII NlaIV PleI RsaI
Sau96I ScrFI Tru9I TseI Tsp509I
TspRI