Restriction map for
pFN21AB3738
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BbsI BciVI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI DraIII EagI EarI EclXI
Eco47III Eco52I EcoO65I FbaI FseI
FspI HincII HindII HpaI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MslI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI SacI SacII
SalI SapI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SspBI
SspI SwaI Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AcyI 454 GGCGCC G[GA]^CG[CT]C
AfaI 844 GTAC GT^AC
AflIII 905 ACACGT A^C[GA][CT]GT
AhdI 518 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 685 CCTCAGG CC^T[ACGT]AGG
AspEI 518 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 433 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 296 CCCGAG C^[CT]CG[GA]G
BamHI 482 GGATCC G^GATCC
BanI 454 GGCGCC G^G[CT][GA]CC
BbeI 454 GGCGCC GGCGC^C
BbrPI 906 CACGTG CAC^GTG
BbvCI 108 GCTGAGG CCTCAGC(-5/-2)
BglII 668 AGATCT A^GATCT
BsaAI 906 CACGTG [CT]AC^GT[GA]
BsaHI 454 GGCGCC G[GA]^CG[CT]C
BsmI 117 GCATTC GAATGC(1/-1)
BsoBI 296 CCCGAG C^[CT]CG[GA]G
BsrDI 580 GCAATG GCAATG(2/0)
BsrFI 389 GCCGGT [GA]^CCGG[CT]
Bsu36I 685 CCTCAGG CC^T[ACGT]AGG
Cfr10I 389 GCCGGT [GA]^CCGG[CT]
CpoI 45 CGGACCG CG^G[AT]CCG
DraI 1012 TTTAAA TTT^AAA
DrdI 606 GACTGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 194 CCATGG C^C[GA][CT]GG
Eam1105I 518 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 685 CCTCAGG CC^T[ACGT]AGG
EcoNI 82 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 310 GAATTC G^AATTC
EcoRV 877 GATATC GAT^ATC
EcoT22I 115 ATGCAT ATGCA^T
FauI 775 GCGGG CCCGC(4/6)
HaeII 454 GGCGCC [GA]GCGC^[CT]
HgaI 934 GCGTC GACGC(5/10)
Hin1I 454 GGCGCC G[GA]^CG[CT]C
KasI 454 GGCGCC G^GCGCC
MaeII 907 ACGT A^CGT
MseI 1013 TTAA T^TAA
MspA1I 715 CAGCTG C[AC]G^C[GT]G
NarI 454 GGCGCC GG^CGCC
NcoI 194 CCATGG C^CATGG
NsiI 115 ATGCAT ATGCA^T
NspI 855 GCATGC [GA]CATG^[CT]
PflFI 433 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 906 CACGTG CAC^GTG
PmlI 906 CACGTG CAC^GTG
PstI 492 CTGCAG CTGCA^G
PvuII 715 CAGCTG CAG^CTG
RsaI 844 GTAC GT^AC
RsrII 45 CGGACCG CG^G[AT]CCG
SbfI 491 CCTGCAGG CCTGCA^GG
SexAI 261 ACCAGGT A^CC[AT]GGT
SfcI 492 CTGCAG C^T[GA][CT]AG
SfoI 454 GGCGCC GGC^GCC
SphI 855 GCATGC GCATG^C
Sse8387I 491 CCTGCAGG CCTGCA^GG
Tru9I 1013 TTAA T^TAA
Tth111I 433 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 863 CCATCCGGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 174 GAATTT [GA]^AATT[CT]
310 GAATTC
Alw44I 40 GTGCAC G^TGCAC
747 GTGCAC
ApaLI 40 GTGCAC G^TGCAC
747 GTGCAC
ApoI 174 GAATTT [GA]^AATT[CT]
310 GAATTC
BalI 572 TGGCCA TGG^CCA
910 TGGCCA
BanII 681 GAGCCC G[GA]GC[CT]^C
825 GGGCTC
BcgI 282 CGAGCCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
920 CGATTTCGATGC
BcnI 49 CCGGG CC^[GC]GG
867 CCGGG
BssSI 568 CTCGTG CACGAG(-5/-1)
808 CTCGTG
BstXI 194 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
575 CCAAAGCAATGG
BstYI 482 GGATCC [GA]^GATC[CT]
668 AGATCT
CfoI 60 GCGC GCG^C
455 GCGC
DraII 524 AGGTCCT [GA]G^G[ACGT]CC[CT]
802 GGGACCC
EcoO109I 524 AGGTCCT [GA]G^G[ACGT]CC[CT]
802 GGGACCC
HhaI 60 GCGC GCG^C
455 GCGC
HinP1I 60 GCGC G^CGC
455 GCGC
HindIII 212 AAGCTT A^AGCTT
424 AAGCTT
HinfI 128 GAATC G^A[ACGT]TC
1065 GAATC
MboII 350 TCTTC GAAGA(8/7)
353 TCTTC
MflI 482 GGATCC [GA]^GATC[CT]
668 AGATCT
MluNI 572 TGGCCA TGG^CCA
910 TGGCCA
MscI 572 TGGCCA TGG^CCA
910 TGGCCA
NciI 49 CCGGG CC^[GC]GG
867 CCGGG
PpuMI 524 AGGTCCT [GA]G^G[AT]CC[CT]
802 GGGACCC
SmlI 593 CTCAAG C^T[CT][GA]AG
1042 CTCAAG
StuI 761 AGGCCT AGG^CCT
998 AGGCCT
TfiI 128 GAATC G^A[AT]TC
1065 GAATC
TspRI 914 CAGTG CA[GC]TG[ACGT][ACGT]^
1056 CAGTG
XhoII 482 GGATCC [GA]^GATC[CT]
668 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeIII MboI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I Tsp509I
TthHB8I