Restriction map for
pFN21AB3738
(Linear) (Six-base) MAPSORT of: KIBB3738.seq Check: 724 from: 1 to: 1071
KIBB3738.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AflIII A'CryG_T
Cuts at: 0 905 1071
Size: 905 166
AhdI GACnn_n'nnGTC
Cuts at: 0 523 1071
Size: 523 548
ApaLI G'TGCA_C
Cuts at: 0 40 747 1071
Size: 40 707 324
Fragments arranged by size:
707 324 40
ApoI r'AATT_y
Cuts at: 0 174 310 1071
Size: 174 136 761
Fragments arranged by size:
761 174 136
AvaI C'yCGr_G
Cuts at: 0 296 1071
Size: 296 775
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 827 860 1071
Size: 827 33 211
Fragments arranged by size:
827 211 33
BamHI G'GATC_C
Cuts at: 0 482 1071
Size: 482 589
BanI G'GyrC_C
Cuts at: 0 454 1071
Size: 454 617
BanII G_rGCy'C
Cuts at: 0 685 829 1071
Size: 685 144 242
Fragments arranged by size:
685 242 144
BbeI G_GCGC'C
Cuts at: 0 458 1071
Size: 458 613
BbvCI CC'TCA_GC
Cuts at: 0 109 1071
Size: 109 962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 271 305 909 943 1071
Size: 271 34 604 34 128
Fragments arranged by size:
604 271 128 34 34
BfrBI ATG'CAT
Cuts at: 0 117 1071
Size: 117 954
BglII A'GATC_T
Cuts at: 0 668 1071
Size: 668 403
Bme1580I G_kGCm'C
Cuts at: 0 44 751 1071
Size: 44 707 320
Fragments arranged by size:
707 320 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 162 1049 1071
Size: 162 887 22
Fragments arranged by size:
887 162 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 649 1003 1015 1071
Size: 85 564 354 12 56
Fragments arranged by size:
564 354 85 56 12
Bpu10I CC'TnA_GC
Cuts at: 0 109 1071
Size: 109 962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 1027 1071
Size: 578 449 44
Fragments arranged by size:
578 449 44
BsaAI yAC'GTr
Cuts at: 0 908 1071
Size: 908 163
BsaHI Gr'CG_yC
Cuts at: 0 455 1071
Size: 455 616
BseYI C'CCAG_C
Cuts at: 0 201 541 717 822 1071
Size: 201 340 176 105 249
Fragments arranged by size:
340 249 201 176 105
BsiHKAI G_wGCw'C
Cuts at: 0 44 294 751 1071
Size: 44 250 457 320
Fragments arranged by size:
457 320 250 44
BsmI GAATG_Cn'
Cuts at: 0 117 1071
Size: 117 954
Bsp1286I G_dGCh'C
Cuts at: 0 44 294 685 751 829 1071
Size: 44 250 391 66 78 242
Fragments arranged by size:
391 250 242 78 66 44
BsrDI GCAATG_nn'
Cuts at: 0 587 1071
Size: 587 484
BsrFI r'CCGG_y
Cuts at: 0 389 1071
Size: 389 682
BssSI C'ACGA_G
Cuts at: 0 568 808 1071
Size: 568 240 263
Fragments arranged by size:
568 263 240
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 582 1071
Size: 201 381 489
Fragments arranged by size:
489 381 201
BstYI r'GATC_y
Cuts at: 0 482 668 1071
Size: 482 186 403
Fragments arranged by size:
482 403 186
Bsu36I CC'TnA_GG
Cuts at: 0 686 1071
Size: 686 385
BtgI C'CryG_G
Cuts at: 0 194 1071
Size: 194 877
DraI TTT'AAA
Cuts at: 0 1014 1071
Size: 1014 57
DrdI GACnn_nn'nnGTC
Cuts at: 0 612 1071
Size: 612 459
EaeI y'GGCC_r
Cuts at: 0 11 572 910 1071
Size: 11 561 338 161
Fragments arranged by size:
561 338 161 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 518 1071
Size: 518 553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 649 1003 1015 1071
Size: 85 564 354 12 56
Fragments arranged by size:
564 354 85 56 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 86 1071
Size: 86 985
EcoO109I rG'GnC_Cy
Cuts at: 0 525 803 1071
Size: 525 278 268
Fragments arranged by size:
525 278 268
EcoRI G'AATT_C
Cuts at: 0 310 1071
Size: 310 761
EcoRV GAT'ATC
Cuts at: 0 879 1071
Size: 879 192
HaeII r_GCGC'y
Cuts at: 0 458 1071
Size: 458 613
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 509 541 1071
Size: 509 32 530
Fragments arranged by size:
530 509 32
HindIII A'AGCT_T
Cuts at: 0 212 424 1071
Size: 212 212 647
Fragments arranged by size:
647 212 212
KasI G'GCGC_C
Cuts at: 0 454 1071
Size: 454 617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 1071
Size: 105 966
MscI TGG'CCA
Cuts at: 0 574 912 1071
Size: 574 338 159
Fragments arranged by size:
574 338 159
MspA1I CmG'CkG
Cuts at: 0 717 1071
Size: 717 354
NarI GG'CG_CC
Cuts at: 0 455 1071
Size: 455 616
NcoI C'CATG_G
Cuts at: 0 194 1071
Size: 194 877
NsiI A_TGCA'T
Cuts at: 0 119 1071
Size: 119 952
NspI r_CATG'y
Cuts at: 0 859 1071
Size: 859 212
PasI CC'CwG_GG
Cuts at: 0 513 1071
Size: 513 558
PfoI T'CCnGG_A
Cuts at: 0 866 1071
Size: 866 205
PmlI CAC'GTG
Cuts at: 0 908 1071
Size: 908 163
PpuMI rG'GwC_Cy
Cuts at: 0 525 803 1071
Size: 525 278 268
Fragments arranged by size:
525 278 268
PstI C_TGCA'G
Cuts at: 0 496 1071
Size: 496 575
PvuII CAG'CTG
Cuts at: 0 717 1071
Size: 717 354
RsrII CG'GwC_CG
Cuts at: 0 46 1071
Size: 46 1025
SanDI GG'GwC_CC
Cuts at: 0 803 1071
Size: 803 268
SbfI CC_TGCA'GG
Cuts at: 0 496 1071
Size: 496 575
SexAI A'CCwGG_T
Cuts at: 0 261 1071
Size: 261 810
SfcI C'TryA_G
Cuts at: 0 492 1071
Size: 492 579
SfoI GGC'GCC
Cuts at: 0 456 1071
Size: 456 615
SmlI C'TyrA_G
Cuts at: 0 593 1042 1071
Size: 593 449 29
Fragments arranged by size:
593 449 29
SphI G_CATG'C
Cuts at: 0 859 1071
Size: 859 212
StuI AGG'CCT
Cuts at: 0 763 1000 1071
Size: 763 237 71
Fragments arranged by size:
763 237 71
StyI C'CwwG_G
Cuts at: 0 194 638 674 1071
Size: 194 444 36 397
Fragments arranged by size:
444 397 194 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 982 1071
Size: 982 89
Tth111I GACn'n_nGTC
Cuts at: 0 436 1071
Size: 436 635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 870 1071
Size: 870 201
Enzymes that do cut:
AflIII AhdI ApaLI ApoI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BcgI BfrBI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaAI BsaHI BseYI BsiHKAI BsmI
Bsp1286I BsrDI BsrFI BssSI BstXI BstYI Bsu36I BtgI
DraI DrdI EaeI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I HindIII KasI MmeI MscI MspA1I
NarI NcoI NsiI NspI PasI PfoI PmlI PpuMI
PstI PvuII RsrII SanDI SbfI SexAI SfcI SfoI
SmlI SphI StuI StyI TaqII Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvrII BbsI BciVI BclI BglI BlpI BmgBI
BmtI BplI BsaI BsaBI BsaWI BsaXI BseRI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraIII EagI EarI EcoICRI FalI FseI FspI
FspAI HincII HpaI KpnI MfeI MluI MslI NaeI
NdeI NgoMIV NheI NotI NruI PacI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
SacI SacII SalI SapI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TatI TstI
XbaI XhoI XmaI XmnI ZraI