(Circular) (Six-base) MAPSORT of: pFN21AB3737.seq Check: 3425 from: 1 to: 5956 pFN21AB3737 5956 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2160 2770 3227 2160 Size: 610 457 4889 Fragments arranged by size: 4889 610 457 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3166 4019 5403 5671 1632 Size: 1534 853 1384 268 1917 Fragments arranged by size: 1917 1534 1384 853 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AlwNI CAG_nnn'CTG Cuts at: 3699 5271 3699 Size: 1572 4384 ApaI G_GGCC'C Cuts at: 2024 3035 2024 Size: 1011 4945 ApaLI G'TGCA_C Cuts at: 2147 2538 4013 5169 2147 Size: 391 1475 1156 2934 Fragments arranged by size: 2934 1475 1156 391 ApoI r'AATT_y Cuts at: 1493 3265 3491 3643 5752 1493 Size: 1772 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2826 3084 3277 4732 618 Size: 325 217 627 1039 258 193 1455 1842 Fragments arranged by size: 1842 1455 1039 627 325 258 217 193 BanII G_rGCy'C Cuts at: 1391 2024 3035 3231 3275 1391 Size: 633 1011 196 44 4072 Fragments arranged by size: 4072 1011 633 196 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4985 BbvCI CC'TCA_GC Cuts at: 2119 2119 Size: 5956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2545 3848 5058 2545 Size: 1303 1210 3443 Fragments arranged by size: 3443 1303 1210 BclI T'GATC_A Cuts at: 1548 3042 1548 Size: 1494 4462 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2453 4567 136 Size: 107 122 71 631 605 781 2114 1525 Fragments arranged by size: 2114 1525 781 631 605 122 107 71 BglII A'GATC_T Cuts at: 2876 5951 2876 Size: 3075 2881 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2024 2151 2270 2464 2542 2725 2829 3035 Size: 234 127 119 194 78 183 104 206 Cuts at: 3035 3109 4017 5173 1790 Size: 74 908 1156 2573 Fragments arranged by size: 2573 1156 908 234 206 194 183 127 119 104 78 74 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2027 2428 3208 Size: 416 53 83 399 24 610 401 780 Cuts at: 3208 3687 4645 442 Size: 479 958 1753 Fragments arranged by size: 1753 958 780 610 479 416 401 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2701 2816 2962 4616 1239 Size: 1462 115 146 1654 2579 Fragments arranged by size: 2579 1654 1462 146 115 Bpu10I CC'TnA_GC Cuts at: 2119 2969 2119 Size: 850 5106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2347 4619 882 Size: 617 848 2272 2219 Fragments arranged by size: 2272 2219 848 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3763 4145 4780 275 Size: 53 83 186 936 2230 382 635 1451 Fragments arranged by size: 2230 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2871 4389 4787 5061 5208 5619 2871 Size: 1518 398 274 147 411 3208 Fragments arranged by size: 3208 1518 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2381 2411 1178 Size: 30 165 30 978 30 4723 Fragments arranged by size: 4723 978 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 2813 3002 3005 1186 Size: 1219 408 189 3 4137 Fragments arranged by size: 4137 1219 408 189 3 BseYI C'CCAG_C Cuts at: 2023 2035 2371 2411 2611 2732 3204 5159 5655 Size: 12 336 40 200 121 472 1955 496 Cuts at: 5655 2023 Size: 2324 Fragments arranged by size: 2324 1955 496 472 336 200 121 40 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2930 2949 1163 Size: 573 1194 19 4170 Fragments arranged by size: 4170 1194 573 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2659 3406 4167 5195 1540 Size: 275 186 658 747 761 1028 2301 Fragments arranged by size: 2301 1028 761 747 658 275 186 BsiHKAI G_wGCw'C Cuts at: 2151 2542 2958 4017 4102 5173 2151 Size: 391 416 1059 85 1071 2934 Fragments arranged by size: 2934 1071 1059 416 391 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2151 2270 2464 2542 2725 2829 Size: 399 234 127 119 194 78 183 104 Cuts at: 2829 2958 3035 3109 3231 3275 4017 4102 5173 Size: 129 77 74 122 44 742 85 1071 Cuts at: 5173 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 234 194 183 129 127 122 119 104 85 78 77 74 44 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2160 2458 2770 3227 3311 844 Size: 1316 298 312 457 84 3489 Fragments arranged by size: 3489 1316 457 312 298 84 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2629 4451 4625 65 Size: 1183 1381 1822 174 1396 Fragments arranged by size: 1822 1396 1381 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2654 2945 4600 4787 1700 Size: 110 844 291 1655 187 2869 Fragments arranged by size: 2869 1655 844 291 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 2327 794 Size: 1533 4423 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2017 2017 Size: 5956 BstYI r'GATC_y Cuts at: 1972 2337 2876 3115 3286 3653 4038 4055 5507 Size: 365 539 239 171 367 385 17 1452 Cuts at: 5507 5593 5605 5951 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 539 385 367 365 346 239 171 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2490 2490 Size: 5956 BtgI C'CryG_G Cuts at: 513 1065 2185 2584 3088 3094 513 Size: 552 1120 399 504 6 3375 Fragments arranged by size: 3375 1120 552 504 399 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2774 3077 487 Size: 683 56 1548 303 3366 Fragments arranged by size: 3366 1548 683 303 56 BtsI GCAGTG_nn' Cuts at: 2382 3472 4266 4286 2382 Size: 1090 794 20 4052 Fragments arranged by size: 4052 1090 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DraIII CAC_nnn'GTG Cuts at: 2129 2129 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2188 2656 Size: 54 1425 50 275 18 232 126 468 Cuts at: 2656 3091 3149 3403 4292 5921 8 Size: 435 58 254 889 1629 43 Fragments arranged by size: 1629 1425 889 468 435 275 254 232 126 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2656 3403 1537 Size: 275 844 747 4090 Fragments arranged by size: 4090 844 747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2732 3886 1353 Size: 1379 1154 3423 Fragments arranged by size: 3423 1379 1154 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2849 4543 4917 5063 387 Size: 1064 138 1260 1694 374 146 1280 Fragments arranged by size: 1694 1280 1260 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2701 2816 2962 3166 4019 4616 5403 Size: 393 1069 115 146 204 853 597 787 Cuts at: 5403 5671 1239 Size: 268 1524 Fragments arranged by size: 1524 1069 853 787 597 393 268 204 146 115 EcoNI CCTnn'n_nnAGG Cuts at: 2075 2798 2894 2075 Size: 723 96 5137 Fragments arranged by size: 5137 723 96 EcoO109I rG'GnC_Cy Cuts at: 2080 2965 3032 2080 Size: 885 67 5004 Fragments arranged by size: 5004 885 67 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 3103 5103 1678 Size: 1425 2000 2531 Fragments arranged by size: 2531 2000 1425 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3161 3193 4597 4629 4671 Size: 32 135 32 1589 32 1404 32 42 Cuts at: 4671 4703 1373 Size: 32 2626 Fragments arranged by size: 2626 1589 1404 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2670 2809 5070 5254 1305 Size: 1365 139 2261 184 2007 Fragments arranged by size: 2261 2007 1365 184 139 MscI TGG'CCA Cuts at: 10 64 1489 1832 2064 2190 3093 3151 10 Size: 54 1425 343 232 126 903 58 2815 Fragments arranged by size: 2815 1425 903 343 232 126 58 54 MslI CAynn'nnrTG Cuts at: 518 1166 2921 3006 3914 4273 4432 518 Size: 648 1755 85 908 359 159 2042 Fragments arranged by size: 2042 1755 908 648 359 159 85 MspA1I CmG'CkG Cuts at: 1606 2661 3082 3186 3372 4049 5197 5442 1606 Size: 1055 421 104 186 677 1148 245 2120 Fragments arranged by size: 2120 1148 1055 677 421 245 186 104 NaeI GCC'GGC Cuts at: 2947 2947 Size: 5956 NcoI C'CATG_G Cuts at: 513 1065 2584 3088 513 Size: 552 1519 504 3381 Fragments arranged by size: 3381 1519 552 504 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NgoMIV G'CCGG_C Cuts at: 2945 2945 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3314 4859 1132 Size: 97 2085 1545 2229 Fragments arranged by size: 2229 2085 1545 97 PasI CC'CwG_GG Cuts at: 1348 2262 1348 Size: 914 5042 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2618 1108 Size: 1510 4446 PfoI T'CCnGG_A Cuts at: 3118 3118 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2965 2080 Size: 885 5071 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PspOMI G'GGCC_C Cuts at: 2020 3031 2020 Size: 1011 4945 PstI C_TGCA'G Cuts at: 830 3074 3308 830 Size: 2244 234 3478 Fragments arranged by size: 3478 2244 234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 3186 3186 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SexAI A'CCwGG_T Cuts at: 3015 3015 Size: 5956 SfcI C'TryA_G Cuts at: 826 1045 3070 3304 4439 5120 5311 826 Size: 219 2025 234 1135 681 191 1471 Fragments arranged by size: 2025 1471 1135 681 234 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4060 4961 5223 820 Size: 197 926 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 2510 2777 2804 3235 1517 Size: 993 267 27 431 4238 Fragments arranged by size: 4238 993 431 267 27 StyI C'CwwG_G Cuts at: 513 1065 2584 3027 3088 3163 3395 513 Size: 552 1519 443 61 75 232 3074 Fragments arranged by size: 3074 1519 552 443 232 75 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2764 3983 4000 4153 4338 4783 325 Size: 1255 1184 1219 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1219 1184 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4202 5914 96 Size: 274 80 33 51 1443 2225 1712 138 Fragments arranged by size: 2225 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2381 2413 5916 5948 2381 Size: 32 3503 32 2389 Fragments arranged by size: 3503 2389 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3061 3086 3061 Size: 25 5931 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 3135 3179 4085 3135 Size: 44 906 5006 Fragments arranged by size: 5006 906 44 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI