(Circular) (Six-base) MAPSORT of: pFN21AB3671.seq Check: 7278 from: 1 to: 5833 pFN21AB3671 5833 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 2312 3960 4333 1563 Size: 749 1648 373 3063 Fragments arranged by size: 3063 1648 749 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 2372 3896 5280 5548 1632 Size: 618 122 1524 1384 268 1917 Fragments arranged by size: 1917 1524 1384 618 268 122 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 2085 4732 5709 1203 Size: 882 2647 977 1327 Fragments arranged by size: 2647 1327 977 882 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 2231 1166 Size: 1065 4768 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3043 1184 Size: 1859 3974 AlwNI CAG_nnn'CTG Cuts at: 2176 2257 2653 3051 3576 5148 2176 Size: 81 396 398 525 1572 2861 Fragments arranged by size: 2861 1572 525 398 396 81 ApaLI G'TGCA_C Cuts at: 2948 3890 5046 2948 Size: 942 1156 3735 Fragments arranged by size: 3735 1156 942 ApoI r'AATT_y Cuts at: 1493 2816 3142 3368 3520 5629 1493 Size: 1323 326 226 152 2109 1697 Fragments arranged by size: 2109 1697 1323 326 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 3158 1870 Size: 73 1215 4545 Fragments arranged by size: 4545 1215 73 AvrII C'CTAG_G Cuts at: 2134 2134 Size: 5833 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2981 3154 4609 618 Size: 325 217 627 1194 173 1455 1842 Fragments arranged by size: 1842 1455 1194 627 325 217 173 BanII G_rGCy'C Cuts at: 1391 2721 3152 1391 Size: 1330 431 4072 Fragments arranged by size: 4072 1330 431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 2321 928 Size: 971 303 119 4440 Fragments arranged by size: 4440 971 303 119 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4024 4058 1903 Size: 34 2087 34 3678 Fragments arranged by size: 3678 2087 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4444 136 Size: 107 122 71 631 605 2772 1525 Fragments arranged by size: 2772 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5828 5828 Size: 5833 BlpI GC'TnA_GC Cuts at: 2346 2681 2720 3250 2346 Size: 335 39 530 4929 Fragments arranged by size: 4929 530 335 39 Bme1580I G_kGCm'C Cuts at: 1790 2952 3894 5050 1790 Size: 1162 942 1156 2573 Fragments arranged by size: 2573 1162 1156 942 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4522 442 Size: 416 53 83 399 24 2147 958 1753 Fragments arranged by size: 2147 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2675 4493 1239 Size: 1436 1818 2579 Fragments arranged by size: 2579 1818 1436 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3958 4823 5121 3958 Size: 865 298 4670 Fragments arranged by size: 4670 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2416 2919 4496 882 Size: 617 917 503 1577 2219 Fragments arranged by size: 2219 1577 917 617 503 BsaAI yAC'GTr Cuts at: 493 1204 2629 3624 493 Size: 711 1425 995 2702 Fragments arranged by size: 2702 1425 995 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3640 4022 4657 275 Size: 53 83 186 936 2107 382 635 1451 Fragments arranged by size: 2107 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2597 2627 1178 Size: 30 165 30 1194 30 4384 Fragments arranged by size: 4384 1194 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2101 2588 1186 Size: 915 487 4431 Fragments arranged by size: 4431 915 487 BseYI C'CCAG_C Cuts at: 5036 5532 5036 Size: 496 5337 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2609 1163 Size: 573 873 4387 Fragments arranged by size: 4387 873 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2721 2952 3894 3979 5050 2721 Size: 231 942 85 1071 3504 Fragments arranged by size: 3504 1071 942 231 85 BsmI GAATG_Cn' Cuts at: 3102 3344 3437 3102 Size: 242 93 5498 Fragments arranged by size: 5498 242 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2721 2952 3152 3894 3979 5050 1391 Size: 399 931 231 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 931 742 399 231 200 85 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2265 3108 3188 844 Size: 1421 843 80 3489 Fragments arranged by size: 3489 1421 843 80 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2916 4328 4502 65 Size: 1183 1668 1412 174 1396 Fragments arranged by size: 1668 1412 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4477 4664 1700 Size: 110 2667 187 2869 Fragments arranged by size: 2869 2667 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 2641 2643 1925 Size: 716 2 5115 Fragments arranged by size: 5115 716 2 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5833 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstYI r'GATC_y Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828 Size: 1191 367 385 17 1452 86 12 346 Cuts at: 5828 1972 Size: 1977 Fragments arranged by size: 1977 1452 1191 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2124 513 Size: 552 939 120 4222 Fragments arranged by size: 4222 939 552 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3111 487 Size: 683 56 1885 3209 Fragments arranged by size: 3209 1885 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4143 4163 3349 Size: 794 20 5019 Fragments arranged by size: 5019 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 2052 2184 2560 2821 3137 2052 Size: 132 376 261 316 4748 Fragments arranged by size: 4748 376 316 261 132 DraIII CAC_nnn'GTG Cuts at: 2086 2086 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2268 3280 Size: 54 1425 50 275 18 291 147 1012 Cuts at: 3280 4169 5798 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1012 889 291 275 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2376 2433 2780 3763 1353 Size: 1023 57 347 983 3423 Fragments arranged by size: 3423 1023 983 347 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2719 2719 Size: 5833 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2372 2675 3896 4493 5280 5548 Size: 393 618 122 303 1221 597 787 268 Cuts at: 5548 1239 Size: 1524 Fragments arranged by size: 1524 1221 787 618 597 393 303 268 122 EcoNI CCTnn'n_nnAGG Cuts at: 2668 2668 Size: 5833 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2049 2760 2825 2917 2049 Size: 711 65 92 4965 Fragments arranged by size: 4965 711 92 65 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 4980 1678 Size: 3302 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2047 2079 2584 2616 2831 Size: 32 135 32 475 32 505 32 215 Cuts at: 2831 2863 4474 4506 4548 4580 1373 Size: 32 1611 32 42 32 2626 Fragments arranged by size: 2626 1611 505 475 215 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 2460 3432 2460 Size: 972 4861 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2908 4947 5131 1305 Size: 1603 2039 184 2007 Fragments arranged by size: 2039 2007 1603 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2123 10 Size: 54 1425 343 291 3720 Fragments arranged by size: 3720 1425 343 291 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 3791 4150 4309 518 Size: 648 1065 1560 359 159 2042 Fragments arranged by size: 2042 1560 1065 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2650 2918 2993 3249 3926 5074 5319 1606 Size: 1044 268 75 256 677 1148 245 2120 Fragments arranged by size: 2120 1148 1044 677 268 256 245 75 NcoI C'CATG_G Cuts at: 513 1065 2004 2124 513 Size: 552 939 120 4222 Fragments arranged by size: 4222 939 552 120 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 3191 4736 1132 Size: 97 1962 1545 2229 Fragments arranged by size: 2229 1962 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5833 PfoI T'CCnGG_A Cuts at: 2387 2387 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PmlI CAC'GTG Cuts at: 2629 2629 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2931 1613 Size: 1318 4515 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PstI C_TGCA'G Cuts at: 830 2380 3185 830 Size: 1550 805 3478 Fragments arranged by size: 3478 1550 805 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 PvuII CAG'CTG Cuts at: 2650 2650 Size: 5833 SacI G_AGCT'C Cuts at: 2721 2721 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 2791 4081 5793 2791 Size: 1290 1712 2831 Fragments arranged by size: 2831 1712 1290 SfcI C'TryA_G Cuts at: 826 1045 2376 3181 4316 4997 5188 826 Size: 219 1331 805 1135 681 191 1471 Fragments arranged by size: 1471 1331 1135 805 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3937 4838 5100 820 Size: 197 926 1994 901 262 1553 Fragments arranged by size: 1994 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 2004 2124 2134 3272 513 Size: 552 939 120 10 1138 3074 Fragments arranged by size: 3074 1138 939 552 120 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2438 3860 3877 4030 4215 4660 325 Size: 1255 858 1422 17 153 185 445 1498 Fragments arranged by size: 1498 1422 1255 858 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2789 4079 5791 Size: 274 80 33 51 1443 812 1290 1712 Cuts at: 5791 96 Size: 138 Fragments arranged by size: 1712 1443 1290 812 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5793 5825 5793 Size: 32 5801 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 2199 3962 2199 Size: 1763 4070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI