################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI AspEI AviII AvrII BalI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI Tsp509I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 529 GGTACC G^GTACC AccII 286 CGCG CG^CG AflIII 1208 ACGTGT A^C[GA][CT]GT AocI 718 CCTGAGG CC^T[ACGT]AGG ApaI 906 GGGCCC GGGCC^C Asp718I 529 GGTACC G^GTACC AspI 1006 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 217 CCCGAG C^[CT]CG[GA]G BamHI 302 GGATCC G^GATCC BfaI 107 CTAG C^TAG BsaAI 1207 TACGTG [CT]AC^GT[GA] BsaWI 1346 ACCGGA [AT]^CCGG[AT] BsmFI 1301 GGGAC GGGAC(10/14) BsmI 1407 GAATGC GAATGC(1/-1) BsoBI 217 CCCGAG C^[CT]CG[GA]G BsrBI 1495 GAGCGG CCGCTC(-3/-3) BstUI 286 CGCG CG^CG BstXI 903 CCAGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 718 CCTGAGG CC^T[ACGT]AGG DraI 694 TTTAAA TTT^AAA DrdI 787 GACTGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 338 TGGCCG [CT]^GGCC[GA] Eco81I 718 CCTGAGG CC^T[ACGT]AGG EcoNI 499 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 1353 GACGC GACGC(5/10) HindIII 1563 AAGCTT A^AGCTT HphI 60 GGTGA GGTGA(8/7) KpnI 529 GGTACC GGTAC^C MaeI 107 CTAG C^TAG MaeII 1208 ACGT A^CGT MaeIII 739 GTGAC ^GT[ACGT]AC MvnI 286 CGCG CG^CG NdeI 1454 CATATG CA^TATG NspI 735 GCATGT [GA]CATG^[CT] PflFI 1006 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 103 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 1311 GAAGAGC GCTCTTC(1/4) SgrAI 1426 CACCGGCG C[GA]^CCGG[CT]G Tsp45I 739 GTGAC ^GT[GC]AC Tth111I 1006 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 150 CCAGGAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 530 GTAC GT^AC 1164 GTAC AlwNI 238 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG 1330 CAGCACCTG BbsI 844 GAAGAC GAAGAC(2/6) 1599 GAAGAC BcnI 306 CCGGG CC^[GC]GG 1361 CCGGG BglI 947 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1411 GCCAATCTGGC BpuAI 844 GAAGAC GAAGAC(2/6) 1599 GAAGAC BsaI 638 GAGACC GGTCTC(1/5) 795 GGTCTC BsgI 1212 GTGCAG GTGCAG(16/14) 1627 GTGCAG BsrFI 1427 ACCGGC [GA]^CCGG[CT] 1589 ACCGGC BstYI 239 AGATCC [GA]^GATC[CT] 302 GGATCC Cfr10I 1427 ACCGGC [GA]^CCGG[CT] 1589 ACCGGC DsaI 82 CCATGG C^C[GA][CT]GG 344 CCATGG EarI 1111 GAAGAG CTCTTC(1/4) 1311 GAAGAG HaeII 436 GGCGCT [GA]GCGC^[CT] 858 GGCGCT Ksp632I 1111 GAAGAG CTCTTC(1/4) 1311 GAAGAG MflI 239 AGATCC [GA]^GATC[CT] 302 GGATCC MseI 5 TTAA T^TAA 695 TTAA MslI 1426 CACCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1454 CATATGGGTG NciI 306 CCGGG CC^[GC]GG 1361 CCGGG NcoI 82 CCATGG C^CATGG 344 CCATGG RsaI 530 GTAC GT^AC 1164 GTAC SacI 184 GAGCTC GAGCT^C 1077 GAGCTC SbfI 949 CCTGCAGG CCTGCA^GG 1335 CCTGCAGG Sse8387I 949 CCTGCAGG CCTGCA^GG 1335 CCTGCAGG Tru9I 5 TTAA T^TAA 695 TTAA XhoII 239 AGATCC [GA]^GATC[CT] 302 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AluI Alw44I AlwI ApaLI Asp700I AspHI AvaII BanI BanII BbvCI BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI TspRI Van91I XmnI