(Circular) (Six-base) MAPSORT of: pFN21AB7845.seq Check: 9181 from: 1 to: 6337 pFN21AB7845 6337 bp With 182 enzymes: SgfI * March 28, 2012 14:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3334 3348 3392 3334 Size: 14 44 6279 Fragments arranged by size: 6279 44 14 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4147 278 Size: 53 83 186 936 2611 2468 Fragments arranged by size: 2611 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3680 1937 Size: 1743 4594 Acc65I G'GTAC_C Cuts at: 2534 3658 2534 Size: 1124 5213 AclI AA'CG_TT Cuts at: 1563 4464 4837 1563 Size: 2901 373 3063 Fragments arranged by size: 3063 2901 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3223 3307 3388 4400 5784 6052 1632 Size: 1591 84 81 1012 1384 268 1917 Fragments arranged by size: 1917 1591 1384 1012 268 84 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6140 AflIII A'CryG_T Cuts at: 1203 3213 5236 6213 1203 Size: 2010 2023 977 1327 Fragments arranged by size: 2023 2010 1327 977 AgeI A'CCGG_T Cuts at: 5168 5168 Size: 6337 AhdI GACnn_n'nnGTC Cuts at: 5066 5066 Size: 6337 AleI CACnn'nnGTG Cuts at: 1166 3435 1166 Size: 2269 4068 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3453 1184 Size: 2269 4068 AlwNI CAG_nnn'CTG Cuts at: 2248 3340 4080 5652 2248 Size: 1092 740 1572 2933 Fragments arranged by size: 2933 1572 1092 740 ApaI G_GGCC'C Cuts at: 2915 2915 Size: 6337 ApaLI G'TGCA_C Cuts at: 3263 3347 3428 4394 5550 3263 Size: 84 81 966 1156 4050 Fragments arranged by size: 4050 1156 966 84 81 ApoI r'AATT_y Cuts at: 1493 3646 3872 4024 6133 1493 Size: 2153 226 152 2109 1697 Fragments arranged by size: 2153 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4891 160 Size: 872 3859 1606 Fragments arranged by size: 3859 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6337 AvaI C'yCGr_G Cuts at: 1870 1943 2222 3662 1870 Size: 73 279 1440 4545 Fragments arranged by size: 4545 1440 279 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2518 2551 2518 Size: 33 6304 BamHI G'GATC_C Cuts at: 2307 3667 4034 2307 Size: 1360 367 4610 Fragments arranged by size: 4610 1360 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2534 2993 3099 3465 3597 Size: 325 217 627 747 459 106 366 132 Cuts at: 3597 3658 5113 618 Size: 61 1455 1842 Fragments arranged by size: 1842 1455 747 627 459 366 325 217 132 106 61 BanII G_rGCy'C Cuts at: 1391 2193 2915 2922 3086 3656 1391 Size: 802 722 7 164 570 4072 Fragments arranged by size: 4072 802 722 570 164 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2856 3611 928 Size: 971 957 755 3654 Fragments arranged by size: 3654 971 957 755 BbvCI CC'TCA_GC Cuts at: 2040 2132 2216 2040 Size: 92 84 6161 Fragments arranged by size: 6161 92 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4528 4562 1903 Size: 34 50 34 2507 34 3678 Fragments arranged by size: 3678 2507 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4229 5439 4229 Size: 1210 5127 BclI T'GATC_A Cuts at: 1548 1548 Size: 6337 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2958 3422 4948 Size: 107 122 71 631 605 1286 464 1526 Cuts at: 4948 136 Size: 1525 Fragments arranged by size: 1526 1525 1286 631 605 464 122 107 71 BglII A'GATC_T Cuts at: 6332 6332 Size: 6337 BlpI GC'TnA_GC Cuts at: 3754 3754 Size: 6337 Bme1580I G_kGCm'C Cuts at: 1790 2915 3056 3267 3351 3432 3442 4398 5554 Size: 1125 141 211 84 81 10 956 1156 Cuts at: 5554 1790 Size: 2573 Fragments arranged by size: 2573 1156 1125 956 211 141 84 81 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6337 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3291 4068 5026 Size: 416 53 83 399 24 1874 777 958 Cuts at: 5026 442 Size: 1753 Fragments arranged by size: 1874 1753 958 777 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2177 2270 2285 2399 2583 2790 2891 Size: 854 84 93 15 114 184 207 101 Cuts at: 2891 3278 4997 1239 Size: 387 1719 2579 Fragments arranged by size: 2579 1719 854 387 207 184 114 101 93 84 15 Bpu10I CC'TnA_GC Cuts at: 2040 2132 2216 2040 Size: 92 84 6161 Fragments arranged by size: 6161 92 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4462 5327 5625 4462 Size: 865 298 5174 Fragments arranged by size: 5174 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2637 2806 5000 882 Size: 617 1138 169 2194 2219 Fragments arranged by size: 2219 2194 1138 617 169 BsaAI yAC'GTr Cuts at: 493 1204 3214 4128 493 Size: 711 2010 914 2702 Fragments arranged by size: 2702 2010 914 711 BsaBI GATnn'nnATC Cuts at: 6331 6331 Size: 6337 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4144 4526 5161 275 Size: 53 83 186 936 2611 382 635 1451 Fragments arranged by size: 2611 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3351 4770 5168 5442 5589 6000 3351 Size: 1419 398 274 147 411 3688 Fragments arranged by size: 3688 1419 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 1178 Size: 30 165 30 663 30 5419 Fragments arranged by size: 5419 663 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2028 2555 2741 2744 2970 1186 Size: 842 527 186 3 226 4553 Fragments arranged by size: 4553 842 527 226 186 3 BseYI C'CCAG_C Cuts at: 2464 2660 2749 2985 3609 5540 6036 2464 Size: 196 89 236 624 1931 496 2765 Fragments arranged by size: 2765 1931 624 496 236 196 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3238 3653 1163 Size: 573 1502 415 3847 Fragments arranged by size: 3847 1502 573 415 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3787 4548 5576 1540 Size: 275 186 1786 761 1028 2301 Fragments arranged by size: 2301 1786 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 2377 3086 3267 3351 3432 4398 4483 5554 Size: 184 709 181 84 81 966 85 1071 Cuts at: 5554 2193 Size: 2976 Fragments arranged by size: 2976 1071 966 709 184 181 85 84 81 BsmI GAATG_Cn' Cuts at: 3418 3848 3941 3418 Size: 430 93 5814 Fragments arranged by size: 5814 430 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2377 2915 2922 3056 3086 3267 Size: 399 403 184 538 7 134 30 181 Cuts at: 3267 3351 3432 3442 3656 4398 4483 5554 1391 Size: 84 81 10 214 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 538 403 399 214 184 181 134 85 84 81 30 10 7 BspEI T'CCGG_A Cuts at: 6000 6000 Size: 6337 BspHI T'CATG_A Cuts at: 4226 5956 4226 Size: 1730 4607 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3334 3348 3392 3625 3692 844 Size: 2490 14 44 233 67 3489 Fragments arranged by size: 3489 2490 233 67 44 14 BsrBI CCG'CTC Cuts at: 1394 1823 3502 4224 1394 Size: 429 1679 722 3507 Fragments arranged by size: 3507 1679 722 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4832 5006 65 Size: 1183 3584 174 1396 Fragments arranged by size: 3584 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3432 3594 4981 5168 1700 Size: 110 1622 162 1387 187 2869 Fragments arranged by size: 2869 1622 1387 187 162 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6337 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6337 BssSI C'ACGA_G Cuts at: 4397 5409 4397 Size: 1012 5325 BstAPI GCAn_nnn'nTGC Cuts at: 794 2963 3225 3340 794 Size: 2169 262 115 3791 Fragments arranged by size: 3791 2169 262 115 BstBI TT'CG_AA Cuts at: 5141 5141 Size: 6337 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6337 BstXI CCAn_nnnn'nTGG Cuts at: 2915 2915 Size: 6337 BstYI r'GATC_y Cuts at: 1972 2244 2307 3667 4034 4419 4436 5888 5974 Size: 272 63 1360 367 385 17 1452 86 Cuts at: 5974 5986 6332 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1360 385 367 346 272 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2724 2724 Size: 6337 BtgI C'CryG_G Cuts at: 513 1065 2087 2349 513 Size: 552 1022 262 4501 Fragments arranged by size: 4501 1022 552 262 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5598 Fragments arranged by size: 5598 683 56 BtsI GCAGTG_nn' Cuts at: 2637 2968 3853 4647 4667 2637 Size: 331 885 794 20 4307 Fragments arranged by size: 4307 885 794 331 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6337 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6302 DraI TTT'AAA Cuts at: 2701 3641 2701 Size: 940 5397 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2798 5344 809 Size: 507 1482 2546 1802 Fragments arranged by size: 2546 1802 1482 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2343 3784 4673 Size: 54 1425 50 275 18 513 1441 889 Cuts at: 4673 6302 8 Size: 1629 43 Fragments arranged by size: 1629 1441 1425 889 513 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3784 1537 Size: 275 1972 4090 Fragments arranged by size: 4090 1972 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3111 3311 4267 1353 Size: 1758 200 956 3423 Fragments arranged by size: 3423 1758 956 200 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2045 4924 5298 5444 387 Size: 1064 138 456 2879 374 146 1280 Fragments arranged by size: 2879 1280 1064 456 374 146 138 EcoICRI GAG'CTC Cuts at: 2191 3084 2191 Size: 893 5444 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2177 2270 2285 2399 2583 2790 Size: 393 461 84 93 15 114 184 207 Cuts at: 2790 2891 3223 3278 3307 3388 4400 4997 5784 Size: 101 332 55 29 81 1012 597 787 Cuts at: 5784 6052 1239 Size: 268 1524 Fragments arranged by size: 1524 1012 787 597 461 393 332 268 207 184 114 101 93 84 81 55 29 15 EcoNI CCTnn'n_nnAGG Cuts at: 2508 2508 Size: 6337 EcoO109I rG'GnC_Cy Cuts at: 2107 2338 2359 2557 2615 2911 2912 3207 2107 Size: 231 21 198 58 296 1 295 5237 Fragments arranged by size: 5237 296 295 231 198 58 21 1 EcoRI G'AATT_C Cuts at: 3646 4024 3646 Size: 378 5959 FspI TGC'GCA Cuts at: 4843 4843 Size: 6337 HaeII r_GCGC'y Cuts at: 1678 2445 2867 5484 1678 Size: 767 422 2617 2531 Fragments arranged by size: 2617 2531 767 422 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3073 3105 4978 5010 5052 Size: 32 135 32 1501 32 1873 32 42 Cuts at: 5052 5084 1373 Size: 32 2626 Fragments arranged by size: 2626 1873 1501 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3681 3927 669 Size: 988 281 1743 246 3079 Fragments arranged by size: 3079 1743 988 281 246 HindIII A'AGCT_T Cuts at: 748 3568 748 Size: 2820 3517 HpaI GTT'AAC Cuts at: 3927 3927 Size: 6337 KpnI G_GTAC'C Cuts at: 2538 3662 2538 Size: 1124 5213 MfeI C'AATT_G Cuts at: 3936 3936 Size: 6337 MluI A'CGCG_T Cuts at: 6213 6213 Size: 6337 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3059 3535 5451 5635 1305 Size: 1754 476 1916 184 2007 Fragments arranged by size: 2007 1916 1754 476 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4515 Fragments arranged by size: 4515 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3435 3463 4295 4654 4813 518 Size: 648 2269 28 832 359 159 2042 Fragments arranged by size: 2269 2042 832 648 359 159 28 MspA1I CmG'CkG Cuts at: 1606 2165 2464 2660 3628 3753 4430 5578 5823 Size: 559 299 196 968 125 677 1148 245 Cuts at: 5823 1606 Size: 2120 Fragments arranged by size: 2120 1148 968 677 559 299 245 196 125 NcoI C'CATG_G Cuts at: 513 1065 2087 2349 513 Size: 552 1022 262 4501 Fragments arranged by size: 4501 1022 552 262 NdeI CA'TA_TG Cuts at: 387 3460 387 Size: 3073 3264 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6337 NotI GC'GGCC_GC Cuts at: 3784 3784 Size: 6337 NspI r_CATG'y Cuts at: 1132 1229 2744 3695 5240 1132 Size: 97 1515 951 1545 2229 Fragments arranged by size: 2229 1545 1515 951 97 PasI CC'CwG_GG Cuts at: 1348 2504 2915 1348 Size: 1156 411 4770 Fragments arranged by size: 4770 1156 411 PciI A'CATG_T Cuts at: 5236 5236 Size: 6337 PflMI CCAn_nnn'nTGG Cuts at: 1108 2330 2653 2813 1108 Size: 1222 323 160 4632 Fragments arranged by size: 4632 1222 323 160 PfoI T'CCnGG_A Cuts at: 2193 2247 2310 2583 2193 Size: 54 63 273 5947 Fragments arranged by size: 5947 273 63 54 PmeI GTTT'AAAC Cuts at: 3641 3641 Size: 6337 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4406 4438 5919 5951 4406 Size: 32 1481 32 4792 Fragments arranged by size: 4792 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2107 2359 2557 2615 2107 Size: 252 198 58 5829 Fragments arranged by size: 5829 252 198 58 PshAI GACnn'nnGTC Cuts at: 1613 2112 1613 Size: 499 5838 PsiI TTA'TAA Cuts at: 3907 3907 Size: 6337 PspOMI G'GGCC_C Cuts at: 2911 2911 Size: 6337 PstI C_TGCA'G Cuts at: 830 2610 2798 2959 3345 3689 830 Size: 1780 188 161 386 344 3478 Fragments arranged by size: 3478 1780 386 344 188 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6337 PvuII CAG'CTG Cuts at: 2165 2464 2660 2165 Size: 299 196 5842 Fragments arranged by size: 5842 299 196 SacI G_AGCT'C Cuts at: 2193 3086 2193 Size: 893 5444 SalI G'TCGA_C Cuts at: 1936 3679 1936 Size: 1743 4594 SapI GCTCTTCn'nnn_ Cuts at: 3311 3311 Size: 6337 SbfI CC_TGCA'GG Cuts at: 2959 3345 3689 2959 Size: 386 344 5607 Fragments arranged by size: 5607 386 344 ScaI AGT'ACT Cuts at: 4585 6297 4585 Size: 1712 4625 SfcI C'TryA_G Cuts at: 826 1045 2606 2794 2955 3341 3685 4820 5501 Size: 219 1561 188 161 386 344 1135 681 Cuts at: 5501 5692 826 Size: 191 1471 Fragments arranged by size: 1561 1471 1135 681 386 344 219 191 188 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6337 SgrAI Cr'CCGG_yG Cuts at: 1700 3432 1700 Size: 1732 4605 SmaI CCC'GGG Cuts at: 1872 3664 1872 Size: 1792 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4441 5342 5604 820 Size: 197 926 2498 901 262 1553 Fragments arranged by size: 2498 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4128 493 Size: 3635 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6337 SphI G_CATG'C Cuts at: 1132 3695 1132 Size: 2563 3774 SspI AAT'ATT Cuts at: 5 52 4261 5 Size: 47 4209 2081 Fragments arranged by size: 4209 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6337 StyI C'CwwG_G Cuts at: 513 1065 2087 2235 2349 2385 3776 513 Size: 552 1022 148 114 36 1391 3074 Fragments arranged by size: 3074 1391 1022 552 148 114 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3453 4364 4381 4534 4719 5164 325 Size: 1255 1873 911 17 153 185 445 1498 Fragments arranged by size: 1873 1498 1255 911 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4583 6295 96 Size: 274 80 33 51 1443 2606 1712 138 Fragments arranged by size: 2606 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2565 2597 6297 6329 2565 Size: 32 3700 32 2573 Fragments arranged by size: 3700 2573 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3014 1316 Size: 1698 4639 XbaI T'CTAG_A Cuts at: 3673 3673 Size: 6337 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2162 2162 Size: 6337 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6337 XmaI C'CCGG_G Cuts at: 1870 3662 1870 Size: 1792 4545 XmnI GAAnn'nnTTC Cuts at: 2514 3044 3071 3236 3320 3485 4466 2514 Size: 530 27 165 84 165 981 4385 Fragments arranged by size: 4385 981 530 165 165 84 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4145 276 Size: 53 83 186 936 2611 2468 Fragments arranged by size: 2611 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI