Restriction map for
pFN21AB7845
(Linear) (Six-base) MAPSORT of: KIBB7845.seq Check: 4179 from: 1 to: 1632
KIBB7845.orf
With 182 enzymes: SgfI *
March 22, 2012 15:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1329 1343 1387 1632
Size: 1329 14 44 245
Fragments arranged by size:
1329 245 44 14
Acc65I G'GTAC_C
Cuts at: 0 529 1632
Size: 529 1103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1218 1302 1383 1632
Size: 1218 84 81 249
Fragments arranged by size:
1218 249 84 81
AflIII A'CryG_T
Cuts at: 0 1208 1632
Size: 1208 424
AleI CACnn'nnGTG
Cuts at: 0 1430 1632
Size: 1430 202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1448 1632
Size: 1448 184
AlwNI CAG_nnn'CTG
Cuts at: 0 243 1335 1632
Size: 243 1092 297
Fragments arranged by size:
1092 297 243
ApaI G_GGCC'C
Cuts at: 0 910 1632
Size: 910 722
ApaLI G'TGCA_C
Cuts at: 0 1258 1342 1423 1632
Size: 1258 84 81 209
Fragments arranged by size:
1258 209 84 81
AvaI C'yCGr_G
Cuts at: 0 217 1632
Size: 217 1415
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 513 546 1632
Size: 513 33 1086
Fragments arranged by size:
1086 513 33
BamHI G'GATC_C
Cuts at: 0 302 1632
Size: 302 1330
BanI G'GyrC_C
Cuts at: 0 529 988 1094 1460 1592 1632
Size: 529 459 106 366 132 40
Fragments arranged by size:
529 459 366 132 106 40
BanII G_rGCy'C
Cuts at: 0 188 910 917 1081 1632
Size: 188 722 7 164 551
Fragments arranged by size:
722 551 188 164 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 851 1606 1632
Size: 851 755 26
Fragments arranged by size:
851 755 26
BbvCI CC'TCA_GC
Cuts at: 0 35 127 211 1632
Size: 35 92 84 1421
Fragments arranged by size:
1421 92 84 35
BglI GCCn_nnn'nGGC
Cuts at: 0 953 1417 1632
Size: 953 464 215
Fragments arranged by size:
953 464 215
Bme1580I G_kGCm'C
Cuts at: 0 910 1051 1262 1346 1427 1437 1632
Size: 910 141 211 84 81 10 195
Fragments arranged by size:
910 211 195 141 84 81 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 1286 1632
Size: 1286 346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 172 265 280 394 578 785 886
Size: 88 84 93 15 114 184 207 101
Cuts at: 886 1273 1632
Size: 387 359
Fragments arranged by size:
387 359 207 184 114 101 93 88
84 15
Bpu10I CC'TnA_GC
Cuts at: 0 35 127 211 1632
Size: 35 92 84 1421
Fragments arranged by size:
1421 92 84 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 632 801 1632
Size: 632 169 831
Fragments arranged by size:
831 632 169
BsaAI yAC'GTr
Cuts at: 0 1209 1632
Size: 1209 423
BsaWI w'CCGG_w
Cuts at: 0 1346 1632
Size: 1346 286
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 1632
Size: 61 30 1541
Fragments arranged by size:
1541 61 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 23 550 736 739 965 1632
Size: 23 527 186 3 226 667
Fragments arranged by size:
667 527 226 186 23 3
BseYI C'CCAG_C
Cuts at: 0 459 655 744 980 1604 1632
Size: 459 196 89 236 624 28
Fragments arranged by size:
624 459 236 196 89 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1233 1632
Size: 1233 399
BsiHKAI G_wGCw'C
Cuts at: 0 188 372 1081 1262 1346 1427 1632
Size: 188 184 709 181 84 81 205
Fragments arranged by size:
709 205 188 184 181 84 81
BsmI GAATG_Cn'
Cuts at: 0 1413 1632
Size: 1413 219
Bsp1286I G_dGCh'C
Cuts at: 0 188 372 910 917 1051 1081 1262 1346
Size: 188 184 538 7 134 30 181 84
Cuts at: 1346 1427 1437 1632
Size: 81 10 195
Fragments arranged by size:
538 195 188 184 181 134 84 81
30 10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1329 1343 1387 1632
Size: 1329 14 44 245
Fragments arranged by size:
1329 245 44 14
BsrBI CCG'CTC
Cuts at: 0 1497 1632
Size: 1497 135
BsrFI r'CCGG_y
Cuts at: 0 1427 1589 1632
Size: 1427 162 43
Fragments arranged by size:
1427 162 43
BstAPI GCAn_nnn'nTGC
Cuts at: 0 958 1220 1335 1632
Size: 958 262 115 297
Fragments arranged by size:
958 297 262 115
BstXI CCAn_nnnn'nTGG
Cuts at: 0 910 1632
Size: 910 722
BstYI r'GATC_y
Cuts at: 0 239 302 1632
Size: 239 63 1330
Fragments arranged by size:
1330 239 63
Bsu36I CC'TnA_GG
Cuts at: 0 719 1632
Size: 719 913
BtgI C'CryG_G
Cuts at: 0 82 344 1632
Size: 82 262 1288
Fragments arranged by size:
1288 262 82
BtsI GCAGTG_nn'
Cuts at: 0 632 963 1632
Size: 632 331 669
Fragments arranged by size:
669 632 331
DraI TTT'AAA
Cuts at: 0 696 1632
Size: 696 936
DrdI GACnn_nn'nnGTC
Cuts at: 0 793 1632
Size: 793 839
EaeI y'GGCC_r
Cuts at: 0 338 1632
Size: 338 1294
EarI CTCTTCn'nnn_
Cuts at: 0 1106 1306 1632
Size: 1106 200 326
Fragments arranged by size:
1106 326 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 1632
Size: 40 1592
EcoICRI GAG'CTC
Cuts at: 0 186 1079 1632
Size: 186 893 553
Fragments arranged by size:
893 553 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 172 265 280 394 578 785 886
Size: 88 84 93 15 114 184 207 101
Cuts at: 886 1218 1273 1302 1383 1632
Size: 332 55 29 81 249
Fragments arranged by size:
332 249 207 184 114 101 93 88
84 81 55 29 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 503 1632
Size: 503 1129
EcoO109I rG'GnC_Cy
Cuts at: 0 102 333 354 552 610 906 907 1202
Size: 102 231 21 198 58 296 1 295
Cuts at: 1202 1632
Size: 430
Fragments arranged by size:
430 296 295 231 198 102 58 21
1
HaeII r_GCGC'y
Cuts at: 0 440 862 1632
Size: 440 422 770
Fragments arranged by size:
770 440 422
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1632
Size: 1068 32 532
Fragments arranged by size:
1068 532 32
HindIII A'AGCT_T
Cuts at: 0 1563 1632
Size: 1563 69
KpnI G_GTAC'C
Cuts at: 0 533 1632
Size: 533 1099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1054 1530 1632
Size: 1054 476 102
Fragments arranged by size:
1054 476 102
MslI CAynn'nnrTG
Cuts at: 0 1430 1458 1632
Size: 1430 28 174
Fragments arranged by size:
1430 174 28
MspA1I CmG'CkG
Cuts at: 0 160 459 655 1623 1632
Size: 160 299 196 968 9
Fragments arranged by size:
968 299 196 160 9
NcoI C'CATG_G
Cuts at: 0 82 344 1632
Size: 82 262 1288
Fragments arranged by size:
1288 262 82
NdeI CA'TA_TG
Cuts at: 0 1455 1632
Size: 1455 177
NspI r_CATG'y
Cuts at: 0 739 1632
Size: 739 893
PasI CC'CwG_GG
Cuts at: 0 499 910 1632
Size: 499 411 722
Fragments arranged by size:
722 499 411
PflMI CCAn_nnn'nTGG
Cuts at: 0 325 648 808 1632
Size: 325 323 160 824
Fragments arranged by size:
824 325 323 160
PfoI T'CCnGG_A
Cuts at: 0 188 242 305 578 1632
Size: 188 54 63 273 1054
Fragments arranged by size:
1054 273 188 63 54
PpuMI rG'GwC_Cy
Cuts at: 0 102 354 552 610 1632
Size: 102 252 198 58 1022
Fragments arranged by size:
1022 252 198 102 58
PshAI GACnn'nnGTC
Cuts at: 0 107 1632
Size: 107 1525
PspOMI G'GGCC_C
Cuts at: 0 906 1632
Size: 906 726
PstI C_TGCA'G
Cuts at: 0 605 793 954 1340 1632
Size: 605 188 161 386 292
Fragments arranged by size:
605 386 292 188 161
PvuII CAG'CTG
Cuts at: 0 160 459 655 1632
Size: 160 299 196 977
Fragments arranged by size:
977 299 196 160
SacI G_AGCT'C
Cuts at: 0 188 1081 1632
Size: 188 893 551
Fragments arranged by size:
893 551 188
SapI GCTCTTCn'nnn_
Cuts at: 0 1306 1632
Size: 1306 326
SbfI CC_TGCA'GG
Cuts at: 0 954 1340 1632
Size: 954 386 292
Fragments arranged by size:
954 386 292
SfcI C'TryA_G
Cuts at: 0 601 789 950 1336 1632
Size: 601 188 161 386 296
Fragments arranged by size:
601 386 296 188 161
SgrAI Cr'CCGG_yG
Cuts at: 0 1427 1632
Size: 1427 205
StyI C'CwwG_G
Cuts at: 0 82 230 344 380 1632
Size: 82 148 114 36 1252
Fragments arranged by size:
1252 148 114 82 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1448 1632
Size: 1448 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 560 592 1632
Size: 560 32 1040
Fragments arranged by size:
1040 560 32
Tth111I GACn'n_nGTC
Cuts at: 0 1009 1632
Size: 1009 623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 157 1632
Size: 157 1475
XmnI GAAnn'nnTTC
Cuts at: 0 509 1039 1066 1231 1315 1480 1632
Size: 509 530 27 165 84 165 152
Fragments arranged by size:
530 509 165 165 152 84 27
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII KpnI MmeI MslI MspA1I
NcoI NdeI NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuII SacI SapI SbfI SfcI SgrAI
StyI TaqII TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BbeI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI
BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraIII EagI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI MfeI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XhoI XmaI ZraI