(Linear) (Six-base) MAPSORT of: KIEE4565.seq Check: 6248 from: 1 to: 2106 KIEE4565.orf With 182 enzymes: SgfI * March 22, 2012 18:23 .. AccI GT'mk_AC Cuts at: 0 818 1382 1498 2106 Size: 818 564 116 608 Fragments arranged by size: 818 608 564 116 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 818 2068 2106 Size: 818 1250 38 Fragments arranged by size: 1250 818 38 AleI CACnn'nnGTG Cuts at: 0 239 2106 Size: 239 1867 AlwNI CAG_nnn'CTG Cuts at: 0 1986 2106 Size: 1986 120 ApaI G_GGCC'C Cuts at: 0 276 2106 Size: 276 1830 ApoI r'AATT_y Cuts at: 0 161 593 614 882 1624 1933 2106 Size: 161 432 21 268 742 309 173 Fragments arranged by size: 742 432 309 268 173 161 21 AvaI C'yCGr_G Cuts at: 0 31 276 2106 Size: 31 245 1830 Fragments arranged by size: 1830 245 31 BamHI G'GATC_C Cuts at: 0 415 534 2106 Size: 415 119 1572 Fragments arranged by size: 1572 415 119 BanII G_rGCy'C Cuts at: 0 276 2106 Size: 276 1830 BbsI GAAGACnn'nnnn_ Cuts at: 0 1364 1852 2106 Size: 1364 488 254 Fragments arranged by size: 1364 488 254 BbvCI CC'TCA_GC Cuts at: 0 1232 2106 Size: 1232 874 BciVI GTATCCnnnnn_n' Cuts at: 0 518 685 2106 Size: 518 167 1421 Fragments arranged by size: 1421 518 167 BclI T'GATC_A Cuts at: 0 1440 2106 Size: 1440 666 BglI GCCn_nnn'nGGC Cuts at: 0 64 2106 Size: 64 2042 BglII A'GATC_T Cuts at: 0 455 2106 Size: 455 1651 Bme1580I G_kGCm'C Cuts at: 0 77 276 2106 Size: 77 199 1830 Fragments arranged by size: 1830 199 77 BmgBI CAC'GTC Cuts at: 0 72 2106 Size: 72 2034 BmrI ACTGGGnnnn_n' Cuts at: 0 540 2106 Size: 540 1566 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2092 2106 Size: 2092 14 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 323 972 1514 2106 Size: 115 208 649 542 592 Fragments arranged by size: 649 592 542 208 115 Bpu10I CC'TnA_GC Cuts at: 0 1232 2106 Size: 1232 874 BsaAI yAC'GTr Cuts at: 0 1809 2106 Size: 1809 297 BsaBI GATnn'nnATC Cuts at: 0 715 2106 Size: 715 1391 BsaHI Gr'CG_yC Cuts at: 0 230 2034 2106 Size: 230 1804 72 Fragments arranged by size: 1804 230 72 BseYI C'CCAG_C Cuts at: 0 992 2106 Size: 992 1114 BsiHKAI G_wGCw'C Cuts at: 0 1264 2106 Size: 1264 842 BsmI GAATG_Cn' Cuts at: 0 561 701 1253 2106 Size: 561 140 552 853 Fragments arranged by size: 853 561 552 140 Bsp1286I G_dGCh'C Cuts at: 0 77 276 1264 2106 Size: 77 199 988 842 Fragments arranged by size: 988 842 199 77 BspHI T'CATG_A Cuts at: 0 954 2106 Size: 954 1152 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 131 1846 2106 Size: 131 1715 260 Fragments arranged by size: 1715 260 131 BsrDI GCAATG_nn' Cuts at: 0 669 1418 1838 1921 2106 Size: 669 749 420 83 185 Fragments arranged by size: 749 669 420 185 83 BsrGI T'GTAC_A Cuts at: 0 2054 2106 Size: 2054 52 BssSI C'ACGA_G Cuts at: 0 403 2106 Size: 403 1703 BstEII G'GTnAC_C Cuts at: 0 748 2106 Size: 748 1358 BstXI CCAn_nnnn'nTGG Cuts at: 0 93 1325 2106 Size: 93 1232 781 Fragments arranged by size: 1232 781 93 BstYI r'GATC_y Cuts at: 0 415 455 534 2094 2106 Size: 415 40 79 1560 12 Fragments arranged by size: 1560 415 79 40 12 BstZ17I GTA'TAC Cuts at: 0 1383 1499 2106 Size: 1383 116 607 Fragments arranged by size: 1383 607 116 BtgI C'CryG_G Cuts at: 0 20 125 475 580 833 1098 1307 2106 Size: 20 105 350 105 253 265 209 799 Fragments arranged by size: 799 350 265 253 209 105 105 20 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 470 505 2051 2086 2106 Size: 470 35 1546 35 20 Fragments arranged by size: 1546 470 35 35 20 DraI TTT'AAA Cuts at: 0 1350 2106 Size: 1350 756 DraIII CAC_nnn'GTG Cuts at: 0 1208 2106 Size: 1208 898 EaeI y'GGCC_r Cuts at: 0 83 2106 Size: 83 2023 EarI CTCTTCn'nnn_ Cuts at: 0 794 2106 Size: 794 1312 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 323 818 972 1514 2068 2106 Size: 115 208 495 154 542 554 38 Fragments arranged by size: 554 542 495 208 154 115 38 EcoO109I rG'GnC_Cy Cuts at: 0 273 2106 Size: 273 1833 HaeII r_GCGC'y Cuts at: 0 52 1404 2106 Size: 52 1352 702 Fragments arranged by size: 1352 702 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 447 479 942 974 1225 1257 1425 1457 Size: 447 32 463 32 251 32 168 32 Cuts at: 1457 2106 Size: 649 Fragments arranged by size: 649 463 447 251 168 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 762 2106 Size: 762 1344 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 298 1016 1893 2106 Size: 298 718 877 213 Fragments arranged by size: 877 718 298 213 MscI TGG'CCA Cuts at: 0 85 2106 Size: 85 2021 MslI CAynn'nnrTG Cuts at: 0 239 548 869 1097 2106 Size: 239 309 321 228 1009 Fragments arranged by size: 1009 321 309 239 228 MspA1I CmG'CkG Cuts at: 0 22 992 1963 2106 Size: 22 970 971 143 Fragments arranged by size: 971 970 143 22 NcoI C'CATG_G Cuts at: 0 833 1098 1307 2106 Size: 833 265 209 799 Fragments arranged by size: 833 799 265 209 NdeI CA'TA_TG Cuts at: 0 549 2106 Size: 549 1557 NspI r_CATG'y Cuts at: 0 662 730 2106 Size: 662 68 1376 Fragments arranged by size: 1376 662 68 PasI CC'CwG_GG Cuts at: 0 1275 2106 Size: 1275 831 PflMI CCAn_nnn'nTGG Cuts at: 0 1104 2106 Size: 1104 1002 PfoI T'CCnGG_A Cuts at: 0 92 2106 Size: 92 2014 PsiI TTA'TAA Cuts at: 0 647 2106 Size: 647 1459 PspOMI G'GGCC_C Cuts at: 0 272 2106 Size: 272 1834 PvuII CAG'CTG Cuts at: 0 992 2106 Size: 992 1114 SacII CC_GC'GG Cuts at: 0 23 2106 Size: 23 2083 SapI GCTCTTCn'nnn_ Cuts at: 0 794 2106 Size: 794 1312 SmaI CCC'GGG Cuts at: 0 278 2106 Size: 278 1828 SpeI A'CTAG_T Cuts at: 0 439 2106 Size: 439 1667 SphI G_CATG'C Cuts at: 0 730 2106 Size: 730 1376 SspI AAT'ATT Cuts at: 0 1138 2106 Size: 1138 968 StuI AGG'CCT Cuts at: 0 686 1804 2106 Size: 686 1118 302 Fragments arranged by size: 1118 686 302 StyI C'CwwG_G Cuts at: 0 687 833 1098 1307 2106 Size: 687 146 265 209 799 Fragments arranged by size: 799 687 265 209 146 TatI w'GTAC_w Cuts at: 0 399 666 2054 2106 Size: 399 267 1388 52 Fragments arranged by size: 1388 399 267 52 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1194 1226 2106 Size: 1194 32 880 Fragments arranged by size: 1194 880 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1282 2106 Size: 1282 824 XmaI C'CCGG_G Cuts at: 0 276 2106 Size: 276 1830 XmnI GAAnn'nnTTC Cuts at: 0 618 2106 Size: 618 1488 Enzymes that do cut: AccI AcuI AleI AlwNI ApaI ApoI AvaI BamHI BanII BbsI BbvCI BciVI BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstEII BstXI BstYI BstZ17I BtgI CspCI DraI DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI NdeI NspI PasI PflMI PfoI PsiI PspOMI PvuII SacII SapI SmaI SpeI SphI SspI StuI StyI TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BfrBI BlpI BmtI BpuEI BsaI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstAPI BstBI Bsu36I BtgZI BtsI ClaI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SrfI SwaI TaqII Tth111I XbaI XhoI ZraI