(Circular) (Six-base) MAPSORT of: pFN21AB3479.seq Check: 4012 from: 1 to: 5968 pFN21AB3479 5968 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2822 2822 Size: 5968 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3778 278 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2219 2942 3311 1937 Size: 282 723 369 4594 Fragments arranged by size: 4594 723 369 282 Acc65I G'GTAC_C Cuts at: 3289 3289 Size: 5968 AclI AA'CG_TT Cuts at: 1563 4095 4468 1563 Size: 2532 373 3063 Fragments arranged by size: 3063 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4031 5415 5683 1632 Size: 2399 1384 268 1917 Fragments arranged by size: 2399 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5771 AflIII A'CryG_T Cuts at: 1203 4867 5844 1203 Size: 3664 977 1327 Fragments arranged by size: 3664 1327 977 AgeI A'CCGG_T Cuts at: 4799 4799 Size: 5968 AhdI GACnn_n'nnGTC Cuts at: 4697 4697 Size: 5968 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5968 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2484 1184 Size: 1300 4668 AlwNI CAG_nnn'CTG Cuts at: 2300 2814 3711 5283 2300 Size: 514 897 1572 2985 Fragments arranged by size: 2985 1572 897 514 ApaLI G'TGCA_C Cuts at: 4025 5181 4025 Size: 1156 4812 ApoI r'AATT_y Cuts at: 1493 2253 3277 3503 3655 5764 1493 Size: 760 1024 226 152 2109 1697 Fragments arranged by size: 2109 1697 1024 760 226 152 AseI AT'TA_AT Cuts at: 160 1032 4522 160 Size: 872 3490 1606 Fragments arranged by size: 3490 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 AvaI C'yCGr_G Cuts at: 1870 1943 3293 1870 Size: 73 1350 4545 Fragments arranged by size: 4545 1350 73 BamHI G'GATC_C Cuts at: 3298 3665 3298 Size: 367 5601 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2787 2967 3289 4744 618 Size: 325 217 627 1000 180 322 1455 1842 Fragments arranged by size: 1842 1455 1000 627 325 322 217 180 BanII G_rGCy'C Cuts at: 1391 2065 2347 3287 1391 Size: 674 282 940 4072 Fragments arranged by size: 4072 940 674 282 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2214 928 Size: 971 315 4682 Fragments arranged by size: 4682 971 315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4159 4193 1903 Size: 34 2222 34 3678 Fragments arranged by size: 3678 2222 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3860 5070 3860 Size: 1210 4758 BclI T'GATC_A Cuts at: 1548 1548 Size: 5968 BfrBI ATG'CAT Cuts at: 3155 3155 Size: 5968 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2765 4579 136 Size: 107 122 71 631 605 1093 1814 1525 Fragments arranged by size: 1814 1525 1093 631 605 122 107 71 BglII A'GATC_T Cuts at: 5963 5963 Size: 5968 BlpI GC'TnA_GC Cuts at: 2685 3385 2685 Size: 700 5268 Bme1580I G_kGCm'C Cuts at: 1790 2434 4029 5185 1790 Size: 644 1595 1156 2573 Fragments arranged by size: 2573 1595 1156 644 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5968 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3699 4657 442 Size: 416 53 83 399 24 2282 958 1753 Fragments arranged by size: 2282 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5968 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2577 2577 Size: 5968 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2134 2652 4628 1239 Size: 895 518 1976 2579 Fragments arranged by size: 2579 1976 895 518 Bpu10I CC'TnA_GC Cuts at: 2038 2038 Size: 5968 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2188 4093 4958 5256 2188 Size: 1905 865 298 2900 Fragments arranged by size: 2900 1905 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4631 882 Size: 617 3132 2219 Fragments arranged by size: 3132 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3025 3759 493 Size: 711 1821 734 2702 Fragments arranged by size: 2702 1821 734 711 BsaBI GATnn'nnATC Cuts at: 5962 5962 Size: 5968 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3775 4157 4792 275 Size: 53 83 186 936 2242 382 635 1451 Fragments arranged by size: 2242 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2439 4401 4799 5073 5220 5631 2439 Size: 1962 398 274 147 411 2776 Fragments arranged by size: 2776 1962 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5743 Fragments arranged by size: 5743 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2337 1186 Size: 1151 4817 BseYI C'CCAG_C Cuts at: 2650 2746 2791 3011 5171 5667 2650 Size: 96 45 220 2160 496 2951 Fragments arranged by size: 2951 2160 496 220 96 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2090 2454 3140 1163 Size: 573 354 364 686 3991 Fragments arranged by size: 3991 686 573 364 354 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3418 4179 5207 1540 Size: 275 186 1417 761 1028 2301 Fragments arranged by size: 2301 1417 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 3179 4029 4114 5185 2691 Size: 488 850 85 1071 3474 Fragments arranged by size: 3474 1071 850 488 85 BsmI GAATG_Cn' Cuts at: 3003 3479 3572 3003 Size: 476 93 5399 Fragments arranged by size: 5399 476 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2065 2347 2434 2691 3179 3287 4029 Size: 399 275 282 87 257 488 108 742 Cuts at: 4029 4114 5185 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 488 399 282 275 257 108 87 85 BspEI T'CCGG_A Cuts at: 5631 5631 Size: 5968 BspHI T'CATG_A Cuts at: 3857 5587 3857 Size: 1730 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2822 3323 844 Size: 1978 501 3489 Fragments arranged by size: 3489 1978 501 BsrBI CCG'CTC Cuts at: 1394 1823 3855 1394 Size: 429 2032 3507 Fragments arranged by size: 3507 2032 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2179 2278 2941 4463 4637 65 Size: 1183 931 99 663 1522 174 1396 Fragments arranged by size: 1522 1396 1183 931 663 174 99 BsrFI r'CCGG_y Cuts at: 1700 1810 4612 4799 1700 Size: 110 2802 187 2869 Fragments arranged by size: 2869 2802 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5968 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5968 BssSI C'ACGA_G Cuts at: 2045 4028 5040 2045 Size: 1983 1012 2973 Fragments arranged by size: 2973 1983 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2383 2814 3057 3192 794 Size: 1589 431 243 135 3570 Fragments arranged by size: 3570 1589 431 243 135 BstBI TT'CG_AA Cuts at: 2100 4772 2100 Size: 2672 3296 BstEII G'GTnAC_C Cuts at: 1184 2741 1184 Size: 1557 4411 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5968 BstYI r'GATC_y Cuts at: 1972 3298 3665 4050 4067 5519 5605 5617 5963 Size: 1326 367 385 17 1452 86 12 346 Cuts at: 5963 1972 Size: 1977 Fragments arranged by size: 1977 1452 1326 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2220 2943 2220 Size: 723 5245 BtgI C'CryG_G Cuts at: 513 1065 2004 2667 513 Size: 552 939 663 3814 Fragments arranged by size: 3814 939 663 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5229 Fragments arranged by size: 5229 683 56 BtsI GCAGTG_nn' Cuts at: 2664 3484 4278 4298 2664 Size: 820 794 20 4334 Fragments arranged by size: 4334 820 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5968 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2212 2247 529 Size: 35 1648 35 4250 Fragments arranged by size: 4250 1648 35 35 DraI TTT'AAA Cuts at: 2073 3272 2073 Size: 1199 4769 DraIII CAC_nnn'GTG Cuts at: 3079 3165 3079 Size: 86 5882 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4975 809 Size: 507 3659 1802 Fragments arranged by size: 3659 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2370 3415 Size: 54 1425 50 275 18 313 227 1045 Cuts at: 3415 4304 5933 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1045 889 313 275 227 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3415 1537 Size: 275 1603 4090 Fragments arranged by size: 4090 1603 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 2172 3251 3898 1353 Size: 792 27 1079 647 3423 Fragments arranged by size: 3423 1079 792 647 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4555 4929 5075 387 Size: 1064 138 2966 374 146 1280 Fragments arranged by size: 2966 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2134 2652 4031 4628 5415 5683 1239 Size: 393 502 518 1379 597 787 268 1524 Fragments arranged by size: 1524 1379 787 597 518 502 393 268 EcoO109I rG'GnC_Cy Cuts at: 2482 2609 2482 Size: 127 5841 EcoRI G'AATT_C Cuts at: 2253 3277 3655 2253 Size: 1024 378 4566 Fragments arranged by size: 4566 1024 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2053 2053 Size: 5968 FspI TGC'GCA Cuts at: 4474 4474 Size: 5968 HaeII r_GCGC'y Cuts at: 1678 5115 1678 Size: 3437 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2663 2695 4609 4641 4683 Size: 32 135 32 1091 32 1914 32 42 Cuts at: 4683 4715 1373 Size: 32 2626 Fragments arranged by size: 2626 1914 1091 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2833 3312 3558 669 Size: 988 281 895 479 246 3079 Fragments arranged by size: 3079 988 895 479 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5968 HpaI GTT'AAC Cuts at: 3558 3558 Size: 5968 KpnI G_GTAC'C Cuts at: 3293 3293 Size: 5968 MfeI C'AATT_G Cuts at: 3567 3567 Size: 5968 MluI A'CGCG_T Cuts at: 5844 5844 Size: 5968 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5082 5266 1305 Size: 3777 184 2007 Fragments arranged by size: 3777 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2145 2372 10 Size: 54 1425 343 313 227 3606 Fragments arranged by size: 3606 1425 343 313 227 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 2883 3926 4285 4444 518 Size: 648 1221 496 1043 359 159 2042 Fragments arranged by size: 2042 1221 1043 648 496 359 159 MspA1I CmG'CkG Cuts at: 1606 2452 3147 3384 4061 5209 5454 1606 Size: 846 695 237 677 1148 245 2120 Fragments arranged by size: 2120 1148 846 695 677 245 237 NcoI C'CATG_G Cuts at: 513 1065 2004 2667 513 Size: 552 939 663 3814 Fragments arranged by size: 3814 939 663 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5968 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5968 NotI GC'GGCC_GC Cuts at: 3415 3415 Size: 5968 NsiI A_TGCA'T Cuts at: 3157 3157 Size: 5968 NspI r_CATG'y Cuts at: 1132 1229 2386 3155 3326 4871 1132 Size: 97 1157 769 171 1545 2229 Fragments arranged by size: 2229 1545 1157 769 171 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5968 PciI A'CATG_T Cuts at: 4867 4867 Size: 5968 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5968 PfoI T'CCnGG_A Cuts at: 2629 2629 Size: 5968 PmeI GTTT'AAAC Cuts at: 3272 3272 Size: 5968 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4037 4069 5550 5582 4037 Size: 32 1481 32 4423 Fragments arranged by size: 4423 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2482 2482 Size: 5968 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5968 PsiI TTA'TAA Cuts at: 3538 3538 Size: 5968 PstI C_TGCA'G Cuts at: 830 2810 3188 3320 830 Size: 1980 378 132 3478 Fragments arranged by size: 3478 1980 378 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5968 PvuII CAG'CTG Cuts at: 2452 3147 2452 Size: 695 5273 SalI G'TCGA_C Cuts at: 1936 3310 1936 Size: 1374 4594 SapI GCTCTTCn'nnn_ Cuts at: 2145 2145 Size: 5968 SbfI CC_TGCA'GG Cuts at: 3320 3320 Size: 5968 ScaI AGT'ACT Cuts at: 4216 5928 4216 Size: 1712 4256 SfcI C'TryA_G Cuts at: 826 1045 2806 3184 3316 4451 5132 5323 826 Size: 219 1761 378 132 1135 681 191 1471 Fragments arranged by size: 1761 1471 1135 681 378 219 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5968 SmaI CCC'GGG Cuts at: 1872 3295 1872 Size: 1423 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2167 4072 4973 5235 820 Size: 197 926 224 1905 901 262 1553 Fragments arranged by size: 1905 1553 926 901 262 224 197 SnaBI TAC'GTA Cuts at: 493 3759 493 Size: 3266 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5968 SphI G_CATG'C Cuts at: 1132 3155 3326 1132 Size: 2023 171 3774 Fragments arranged by size: 3774 2023 171 SspI AAT'ATT Cuts at: 5 52 2705 3892 5 Size: 47 2653 1187 2081 Fragments arranged by size: 2653 2081 1187 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5968 StyI C'CwwG_G Cuts at: 513 1065 2004 2397 2605 2667 3407 513 Size: 552 939 393 208 62 740 3074 Fragments arranged by size: 3074 939 740 552 393 208 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2805 3995 4012 4165 4350 4795 325 Size: 1255 1225 1190 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1225 1190 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 4214 5926 Size: 274 80 33 51 1443 402 1835 1712 Cuts at: 5926 96 Size: 138 Fragments arranged by size: 1835 1712 1443 402 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5928 5960 5928 Size: 32 5936 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5968 XbaI T'CTAG_A Cuts at: 3304 3304 Size: 5968 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5968 XmaI C'CCGG_G Cuts at: 1870 3293 1870 Size: 1423 4545 XmnI GAAnn'nnTTC Cuts at: 2978 4097 2978 Size: 1119 4849 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3776 276 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI