(Circular) (Six-base) MAPSORT of: pFN21AB3401.seq Check: 4599 from: 1 to: 5452 pFN21AB3401 5452 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3262 278 Size: 53 83 186 936 1726 2468 Fragments arranged by size: 2468 1726 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2795 1937 Size: 858 4594 Acc65I G'GTAC_C Cuts at: 2773 2773 Size: 5452 AclI AA'CG_TT Cuts at: 1563 3579 3952 1563 Size: 2016 373 3063 Fragments arranged by size: 3063 2016 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2606 3515 4899 5167 1632 Size: 974 909 1384 268 1917 Fragments arranged by size: 1917 1384 974 909 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5255 AflIII A'CryG_T Cuts at: 1203 2551 4351 5328 1203 Size: 1348 1800 977 1327 Fragments arranged by size: 1800 1348 1327 977 AgeI A'CCGG_T Cuts at: 4283 4283 Size: 5452 AhdI GACnn_n'nnGTC Cuts at: 4181 4181 Size: 5452 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5452 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5452 AlwNI CAG_nnn'CTG Cuts at: 2033 3195 4767 2033 Size: 1162 1572 2718 Fragments arranged by size: 2718 1572 1162 ApaI G_GGCC'C Cuts at: 2408 2408 Size: 5452 ApaLI G'TGCA_C Cuts at: 3509 4665 3509 Size: 1156 4296 ApoI r'AATT_y Cuts at: 1493 2761 2987 3139 5248 1493 Size: 1268 226 152 2109 1697 Fragments arranged by size: 2109 1697 1268 226 152 AseI AT'TA_AT Cuts at: 160 1032 4006 160 Size: 872 2974 1606 Fragments arranged by size: 2974 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5452 AvaI C'yCGr_G Cuts at: 1870 1943 2777 1870 Size: 73 834 4545 Fragments arranged by size: 4545 834 73 AvrII C'CTAG_G Cuts at: 2510 2510 Size: 5452 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2373 2406 2373 Size: 33 5419 BamHI G'GATC_C Cuts at: 2782 3149 2782 Size: 367 5085 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2773 4228 618 Size: 325 217 627 986 1455 1842 Fragments arranged by size: 1842 1455 986 627 325 217 BanII G_rGCy'C Cuts at: 1391 2408 2771 1391 Size: 1017 363 4072 Fragments arranged by size: 4072 1017 363 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3643 3677 1903 Size: 34 1706 34 3678 Fragments arranged by size: 3678 1706 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3344 4554 3344 Size: 1210 4242 BclI T'GATC_A Cuts at: 1548 1548 Size: 5452 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2403 4063 136 Size: 107 122 71 631 605 731 1660 1525 Fragments arranged by size: 1660 1525 731 631 605 122 107 71 BglII A'GATC_T Cuts at: 5447 5447 Size: 5452 BlpI GC'TnA_GC Cuts at: 2869 2869 Size: 5452 Bme1580I G_kGCm'C Cuts at: 1790 2408 3513 4669 1790 Size: 618 1105 1156 2573 Fragments arranged by size: 2573 1156 1105 618 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5452 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3183 4141 442 Size: 416 53 83 399 24 1766 958 1753 Fragments arranged by size: 1766 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2421 1056 Size: 1365 4087 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2292 4112 1239 Size: 1053 1820 2579 Fragments arranged by size: 2579 1820 1053 Bpu10I CC'TnA_GC Cuts at: 2664 2664 Size: 5452 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3577 4442 4740 3577 Size: 865 298 4289 Fragments arranged by size: 4289 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4115 882 Size: 617 2616 2219 Fragments arranged by size: 2616 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2552 3243 493 Size: 711 1348 691 2702 Fragments arranged by size: 2702 1348 711 691 BsaBI GATnn'nnATC Cuts at: 2316 5446 2316 Size: 3130 2322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3259 3641 4276 275 Size: 53 83 186 936 1726 382 635 1451 Fragments arranged by size: 1726 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3885 4283 4557 4704 5115 3885 Size: 398 274 147 411 4222 Fragments arranged by size: 4222 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5227 Fragments arranged by size: 5227 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5452 BseYI C'CCAG_C Cuts at: 2026 4655 5151 2026 Size: 2629 496 2327 Fragments arranged by size: 2629 2327 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4879 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2902 3663 4691 1540 Size: 275 186 901 761 1028 2301 Fragments arranged by size: 2301 1028 901 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3513 3598 4669 3513 Size: 85 1071 4296 Fragments arranged by size: 4296 1071 85 BsmI GAATG_Cn' Cuts at: 2245 2963 3056 2245 Size: 718 93 4641 Fragments arranged by size: 4641 718 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2689 2689 Size: 5452 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2408 2771 3513 3598 4669 1391 Size: 399 618 363 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 618 399 363 85 BspEI T'CCGG_A Cuts at: 5115 5115 Size: 5452 BspHI T'CATG_A Cuts at: 3341 5071 3341 Size: 1730 3722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2807 844 Size: 1963 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3339 1394 Size: 429 1516 3507 Fragments arranged by size: 3507 1516 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3947 4121 65 Size: 1183 2699 174 1396 Fragments arranged by size: 2699 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4096 4283 1700 Size: 110 2286 187 2869 Fragments arranged by size: 2869 2286 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5452 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5452 BssSI C'ACGA_G Cuts at: 3512 4524 3512 Size: 1012 4440 BstAPI GCAn_nnn'nTGC Cuts at: 794 2630 794 Size: 1836 3616 BstBI TT'CG_AA Cuts at: 4256 4256 Size: 5452 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5452 BstYI r'GATC_y Cuts at: 1972 2782 3149 3534 3551 5003 5089 5101 5447 Size: 810 367 385 17 1452 86 12 346 Cuts at: 5447 1972 Size: 1977 Fragments arranged by size: 1977 1452 810 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2212 513 Size: 552 1147 3753 Fragments arranged by size: 3753 1147 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4713 Fragments arranged by size: 4713 683 56 BtsI GCAGTG_nn' Cuts at: 2968 3762 3782 2968 Size: 794 20 4638 Fragments arranged by size: 4638 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5452 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2092 2127 529 Size: 35 1528 35 3854 Fragments arranged by size: 3854 1528 35 35 DraI TTT'AAA Cuts at: 2756 2756 Size: 5452 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4459 809 Size: 507 3143 1802 Fragments arranged by size: 3143 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2215 2643 Size: 54 1425 50 275 18 270 115 428 Cuts at: 2643 2899 3788 5417 8 Size: 256 889 1629 43 Fragments arranged by size: 1629 1425 889 428 275 270 256 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2899 1537 Size: 275 1087 4090 Fragments arranged by size: 4090 1087 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2610 2687 3382 1353 Size: 1257 77 695 3423 Fragments arranged by size: 3423 1257 695 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2213 2453 4039 4413 4559 387 Size: 1064 138 624 240 1586 374 146 1280 Fragments arranged by size: 1586 1280 1064 624 374 240 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 2606 3515 4112 4899 5167 1239 Size: 393 660 314 909 597 787 268 1524 Fragments arranged by size: 1524 909 787 660 597 393 314 268 EcoO109I rG'GnC_Cy Cuts at: 2405 2405 Size: 5452 EcoRI G'AATT_C Cuts at: 2761 3139 2761 Size: 378 5074 FspI TGC'GCA Cuts at: 3958 3958 Size: 5452 HaeII r_GCGC'y Cuts at: 1678 2677 4599 1678 Size: 999 1922 2531 Fragments arranged by size: 2531 1922 999 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2521 2553 4093 4125 4167 Size: 32 135 32 949 32 1540 32 42 Cuts at: 4167 4199 1373 Size: 32 2626 Fragments arranged by size: 2626 1540 949 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2796 3042 669 Size: 988 281 858 246 3079 Fragments arranged by size: 3079 988 858 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5452 HpaI GTT'AAC Cuts at: 3042 3042 Size: 5452 KpnI G_GTAC'C Cuts at: 2777 2777 Size: 5452 MfeI C'AATT_G Cuts at: 3051 3051 Size: 5452 MluI A'CGCG_T Cuts at: 5328 5328 Size: 5452 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4566 4750 1305 Size: 3261 184 2007 Fragments arranged by size: 3261 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2102 2645 10 Size: 54 1425 343 270 543 2817 Fragments arranged by size: 2817 1425 543 343 270 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 2217 3410 3769 3928 518 Size: 648 888 163 1193 359 159 2042 Fragments arranged by size: 2042 1193 888 648 359 163 159 MspA1I CmG'CkG Cuts at: 1606 2868 3545 4693 4938 1606 Size: 1262 677 1148 245 2120 Fragments arranged by size: 2120 1262 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2212 513 Size: 552 1147 3753 Fragments arranged by size: 3753 1147 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5452 NheI G'CTAG_C Cuts at: 1052 2417 1052 Size: 1365 4087 NotI GC'GGCC_GC Cuts at: 2899 2899 Size: 5452 NspI r_CATG'y Cuts at: 1132 1229 2810 4355 1132 Size: 97 1581 1545 2229 Fragments arranged by size: 2229 1581 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5452 PciI A'CATG_T Cuts at: 4351 4351 Size: 5452 PflMI CCAn_nnn'nTGG Cuts at: 1108 2033 1108 Size: 925 4527 PfoI T'CCnGG_A Cuts at: 2368 2368 Size: 5452 PmeI GTTT'AAAC Cuts at: 2756 2756 Size: 5452 PmlI CAC'GTG Cuts at: 2552 2552 Size: 5452 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3521 3553 5034 5066 3521 Size: 32 1481 32 3907 Fragments arranged by size: 3907 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5452 PsiI TTA'TAA Cuts at: 3022 3022 Size: 5452 PspOMI G'GGCC_C Cuts at: 2404 2404 Size: 5452 PstI C_TGCA'G Cuts at: 830 2562 2626 2804 830 Size: 1732 64 178 3478 Fragments arranged by size: 3478 1732 178 64 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5452 SalI G'TCGA_C Cuts at: 1936 2794 1936 Size: 858 4594 SbfI CC_TGCA'GG Cuts at: 2804 2804 Size: 5452 ScaI AGT'ACT Cuts at: 3700 5412 3700 Size: 1712 3740 SfcI C'TryA_G Cuts at: 826 1045 2385 2558 2622 2800 3935 4616 4807 Size: 219 1340 173 64 178 1135 681 191 Cuts at: 4807 826 Size: 1471 Fragments arranged by size: 1471 1340 1135 681 219 191 178 173 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5452 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5452 SmaI CCC'GGG Cuts at: 1872 2779 1872 Size: 907 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3556 4457 4719 820 Size: 197 926 1613 901 262 1553 Fragments arranged by size: 1613 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3243 493 Size: 2750 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5452 SphI G_CATG'C Cuts at: 1132 2810 1132 Size: 1678 3774 SspI AAT'ATT Cuts at: 5 52 3376 5 Size: 47 3324 2081 Fragments arranged by size: 3324 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5452 StyI C'CwwG_G Cuts at: 513 1065 2212 2261 2510 2891 513 Size: 552 1147 49 249 381 3074 Fragments arranged by size: 3074 1147 552 381 249 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2117 2539 3479 3496 3649 3834 4279 Size: 1255 537 422 940 17 153 185 445 Cuts at: 4279 325 Size: 1498 Fragments arranged by size: 1498 1255 940 537 445 422 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3698 5410 96 Size: 274 80 33 51 1443 1721 1712 138 Fragments arranged by size: 1721 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5412 5444 5412 Size: 32 5420 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5452 XbaI T'CTAG_A Cuts at: 2788 2788 Size: 5452 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5452 XmaI C'CCGG_G Cuts at: 1870 2777 1870 Size: 907 4545 XmnI GAAnn'nnTTC Cuts at: 3581 3581 Size: 5452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3260 276 Size: 53 83 186 936 1726 2468 Fragments arranged by size: 2468 1726 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI