################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII AccII AccIII AcyI AhdI Alw44I ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AvrII BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BsaAI BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco52I EcoO109I EcoO65I EcoRI FseI Hin1I KasI KspI MamI MluI MroI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 1055 GTCTAC GT^[AC][GT]AC AclI 1284 AACGTT AA^CGTT AflII 550 CTTAAG C^TTAAG AflIII 169 ACATGT A^C[GA][CT]GT AgeI 985 ACCGGT A^CCGGT AocI 2175 CCTTAGG CC^T[ACGT]AGG Aor51HI 291 AGCGCT AGC^GCT AviII 2258 TGCGCA TGC^GCA BalI 498 TGGCCA TGG^CCA BamHI 2298 GGATCC G^GATCC BfrI 550 CTTAAG C^TTAAG BsaI 325 GGTCTC GGTCTC(1/5) BsaWI 985 ACCGGT [AT]^CCGG[AT] BsgI 2648 GTGCAG GTGCAG(16/14) BspLU11I 169 ACATGT A^CATGT BsrFI 985 ACCGGT [GA]^CCGG[CT] Bsu36I 2175 CCTTAGG CC^T[ACGT]AGG Cfr10I 985 ACCGGT [GA]^CCGG[CT] DrdI 191 GACAGCTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1935 CCATGG C^C[GA][CT]GG EaeI 498 TGGCCA [CT]^GGCC[GA] EarI 3006 GAAGAG CTCTTC(1/4) Eco47III 291 AGCGCT AGC^GCT Eco81I 2175 CCTTAGG CC^T[ACGT]AGG FauI 2752 GCGGG CCCGC(4/6) FspI 2258 TGCGCA TGC^GCA HaeII 291 AGCGCT [GA]GCGC^[CT] HapII 986 CCGG C^CGG HgaI 644 GACGC GACGC(5/10) HindIII 1119 AAGCTT A^AGCTT HpaI 2662 GTTAAC GTT^AAC HpaII 986 CCGG C^CGG Ksp632I 3006 GAAGAG CTCTTC(1/4) MfeI 502 CAATTG C^AATTG MluNI 498 TGGCCA TGG^CCA MscI 498 TGGCCA TGG^CCA MslI 170 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 986 CCGG C^CGG MunI 502 CAATTG C^AATTG NcoI 1935 CCATGG C^CATGG PinAI 985 ACCGGT A^CCGGT Psp1406I 1284 AACGTT AA^CGTT SfcI 1344 CTATAG C^T[GA][CT]AG SpeI 3018 ACTAGT A^CTAGT SphI 2531 GCATGC GCATG^C StuI 1913 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 1034 GGTACC G^GTACC 1354 GGTACC AlwNI 2539 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG 2956 CAGTTTCTG Asp718I 1034 GGTACC G^GTACC 1354 GGTACC BanII 70 GAGCCC G[GA]GC[CT]^C 596 GAGCCC BbsI 2368 GAAGAC GAAGAC(2/6) 472 GTCTTC BglII 718 AGATCT A^GATCT 1193 AGATCT BlpI 1230 GCTGAGC GC^T[ACGT]AGC 2408 GCTTAGC BmyI 70 GAGCCC G[AGT]GC[ACT]^C 596 GAGCCC BpmI 623 CTGGAG CTGGAG(16/14) 803 CTGGAG Bpu1102I 1230 GCTGAGC GC^T[ACGT]AGC 2408 GCTTAGC BpuAI 2368 GAAGAC GAAGAC(2/6) 472 GTCTTC BsmAI 326 GTCTC GTCTC(1/5) 2080 GTCTC Bsp1286I 70 GAGCCC G[AGT]GC[ACT]^C 596 GAGCCC BspMI 228 GCAGGT ACCTGC(4/8) 1440 GCAGGT BstXI 1143 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2385 CCATTTGATTGG CelII 1230 GCTGAGC GC^T[ACGT]AGC 2408 GCTTAGC CfoI 292 GCGC GCG^C 2259 GCGC EcoNI 931 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1605 CCTAGAAAAGG EcoT22I 17 ATGCAT ATGCA^T 369 ATGCAT HhaI 292 GCGC GCG^C 2259 GCGC HinP1I 292 GCGC G^CGC 2259 GCGC HincII 1930 GTCAAC GT[CT]^[GA]AC 2662 GTTAAC HindII 1930 GTCAAC GT[CT]^[GA]AC 2662 GTTAAC KpnI 1034 GGTACC GGTAC^C 1354 GGTACC MaeII 286 ACGT A^CGT 1285 ACGT NsiI 17 ATGCAT ATGCA^T 369 ATGCAT PleI 125 GACTC GAGTC(4/5) 3009 GAGTC ScaI 1626 AGTACT AGT^ACT 1647 AGTACT TaqI 329 TCGA T^CGA 2690 TCGA TthHB8I 329 TCGA T^CGA 2690 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BanI BbvI BclI BfaI BsaJI BseRI BsiYI BslI BsmFI BsmI Bsp1407I BspHI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoRV EcoT14I FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI