Restriction map for
pFN21AB3392
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoT22I FauI
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 463 GTCTAC GT^[AC][GT]AC
AciI 103 GCGG CCGC(-3/-1)
AcsI 16 GAATTC [GA]^AATT[CT]
AfaI 726 GTAC GT^AC
AflIII 656 ACGTGT A^C[GA][CT]GT
AlwI 97 GGATC GGATC(4/5)
ApoI 16 GAATTC [GA]^AATT[CT]
Asp700I 58 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 491 TGGCCA TGG^CCA
BanII 108 GAGCCC G[GA]GC[CT]^C
BbsI 586 GAAGAC GAAGAC(2/6)
BclI 887 TGATCA T^GATCA
BcnI 94 CCCGG CC^[GC]GG
BglII 486 AGATCT A^GATCT
BmyI 108 GAGCCC G[AGT]GC[ACT]^C
BpuAI 586 GAAGAC GAAGAC(2/6)
BseRI 313 GAGGAG GAGGAG(10/8)
BsmAI 449 GAGAC GTCTC(1/5)
BsmI 937 GCATTC GAATGC(1/-1)
Bsp1286I 108 GAGCCC G[AGT]GC[ACT]^C
BsrDI 4 GCAATG GCAATG(2/0)
BstYI 486 AGATCT [GA]^GATC[CT]
Cac8I 27 GCAGGC GC[ACGT]^[ACGT]GC
EaeI 491 TGGCCA [CT]^GGCC[GA]
EcoRI 16 GAATTC G^AATTC
EcoRV 928 GATATC GAT^ATC
EcoT14I 387 CCTTGG C^C[AT][AT]GG
FbaI 887 TGATCA T^GATCA
HapII 95 CCGG C^CGG
HgaI 228 GCGTC GACGC(5/10)
HincII 230 GTCAAC GT[CT]^[GA]AC
HindII 230 GTCAAC GT[CT]^[GA]AC
HindIII 841 AAGCTT A^AGCTT
HinfI 598 GATTC G^A[ACGT]TC
HpaII 95 CCGG C^CGG
MflI 486 AGATCT [GA]^GATC[CT]
MluNI 491 TGGCCA TGG^CCA
MscI 491 TGGCCA TGG^CCA
MspA1I 101 CAGCGG C[AC]G^C[GT]G
MspI 95 CCGG C^CGG
NciI 94 CCCGG CC^[GC]GG
NlaIV 91 GGTCCC GG[ACGT]^[ACGT]CC
RsaI 726 GTAC GT^AC
SapI 49 GAAGAGC GCTCTTC(1/4)
SfcI 465 CTACAG C^T[GA][CT]AG
StuI 29 AGGCCT AGG^CCT
StyI 387 CCTTGG C^C[AT][AT]GG
TfiI 598 GATTC G^A[AT]TC
TspRI 284 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 486 AGATCT [GA]^GATC[CT]
XmnI 58 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 91 GGTCC G^G[AT]CC
756 GGACC
BsmFI 643 GGGAC GGGAC(10/14)
92 GTCCC
BstEII 304 GGTCACC G^GT[ACGT]ACC
556 GGTGACC
BstPI 304 GGTCACC G^GT[ACGT]ACC
556 GGTGACC
CfoI 346 GCGC GCG^C
809 GCGC
DraII 383 AGGGCCT [GA]G^G[ACGT]CC[CT]
610 AGGGCCT
EarI 49 GAAGAG CTCTTC(1/4)
480 GAAGAG
EcoNI 21 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
309 CCTTGAGGAGG
EcoO109I 383 AGGGCCT [GA]G^G[ACGT]CC[CT]
610 AGGGCCT
EcoO65I 304 GGTCACC G^GT[ACGT]ACC
556 GGTGACC
HhaI 346 GCGC GCG^C
809 GCGC
HinP1I 346 GCGC G^CGC
809 GCGC
Ksp632I 49 GAAGAG CTCTTC(1/4)
480 GAAGAG
MaeII 650 ACGT A^CGT
656 ACGT
MseI 170 TTAA T^TAA
455 TTAA
MwoI 809 GCGCCACAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
826 GCTTTCTTTGC
SmlI 22 CTCAAG C^T[CT][GA]AG
310 CTTGAG
Tru9I 170 TTAA T^TAA
455 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BbvI BpmI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HphI ItaI MaeIII MboI
MboII MnlI MvaI NdeII NlaIII
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I Tsp509I