Restriction map for
pFN21AB3392
(Linear) (Six-base) MAPSORT of: KIBB3392.seq Check: 9398 from: 1 to: 1038
KIBB3392.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 464 1038
Size: 464 574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 499 1038
Size: 307 192 539
Fragments arranged by size:
539 307 192
AflIII A'CryG_T
Cuts at: 0 656 1038
Size: 656 382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 371 1038
Size: 371 667
ApoI r'AATT_y
Cuts at: 0 16 1038
Size: 16 1022
BanII G_rGCy'C
Cuts at: 0 112 1038
Size: 112 926
BbsI GAAGACnn'nnnn_
Cuts at: 0 593 1038
Size: 593 445
BclI T'GATC_A
Cuts at: 0 887 1038
Size: 887 151
BglII A'GATC_T
Cuts at: 0 486 1038
Size: 486 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 326 539 781 1038
Size: 238 88 213 242 257
Fragments arranged by size:
257 242 238 213 88
Bpu10I CC'TnA_GC
Cuts at: 0 551 1038
Size: 551 487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 331 1038
Size: 7 324 707
Fragments arranged by size:
707 324 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 1038
Size: 328 710
BsmI GAATG_Cn'
Cuts at: 0 937 1038
Size: 937 101
Bsp1286I G_dGCh'C
Cuts at: 0 112 1038
Size: 112 926
BsrDI GCAATG_nn'
Cuts at: 0 11 1038
Size: 11 1027
BstEII G'GTnAC_C
Cuts at: 0 304 556 1038
Size: 304 252 482
Fragments arranged by size:
482 304 252
BstYI r'GATC_y
Cuts at: 0 486 1038
Size: 486 552
EaeI y'GGCC_r
Cuts at: 0 491 1038
Size: 491 547
EarI CTCTTCn'nnn_
Cuts at: 0 44 475 1038
Size: 44 431 563
Fragments arranged by size:
563 431 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 307 326 499 539 781 1038
Size: 238 69 19 173 40 242 257
Fragments arranged by size:
257 242 238 173 69 40 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 313 1038
Size: 25 288 725
Fragments arranged by size:
725 288 25
EcoO109I rG'GnC_Cy
Cuts at: 0 384 611 1038
Size: 384 227 427
Fragments arranged by size:
427 384 227
EcoRI G'AATT_C
Cuts at: 0 16 1038
Size: 16 1022
EcoRV GAT'ATC
Cuts at: 0 930 1038
Size: 930 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 99 131 490 522 1038
Size: 99 32 359 32 516
Fragments arranged by size:
516 359 99 32 32
HincII GTy'rAC
Cuts at: 0 232 1038
Size: 232 806
HindIII A'AGCT_T
Cuts at: 0 841 1038
Size: 841 197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 846 1038
Size: 846 192
MscI TGG'CCA
Cuts at: 0 493 1038
Size: 493 545
MspA1I CmG'CkG
Cuts at: 0 103 1038
Size: 103 935
PfoI T'CCnGG_A
Cuts at: 0 93 254 980 1038
Size: 93 161 726 58
Fragments arranged by size:
726 161 93 58
SapI GCTCTTCn'nnn_
Cuts at: 0 44 1038
Size: 44 994
SfcI C'TryA_G
Cuts at: 0 465 1038
Size: 465 573
SmlI C'TyrA_G
Cuts at: 0 22 310 1038
Size: 22 288 728
Fragments arranged by size:
728 288 22
StuI AGG'CCT
Cuts at: 0 31 1038
Size: 31 1007
StyI C'CwwG_G
Cuts at: 0 387 1038
Size: 387 651
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 575 1038
Size: 575 463
TatI w'GTAC_w
Cuts at: 0 725 1038
Size: 725 313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 89 121 1038
Size: 89 32 917
Fragments arranged by size:
917 89 32
XmnI GAAnn'nnTTC
Cuts at: 0 62 1038
Size: 62 976
Enzymes that do cut:
AccI AcuI AflIII AlfI ApoI BanII BbsI BclI
BglII BpmI Bpu10I BpuEI BseRI BsmI Bsp1286I BsrDI
BstEII BstYI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV Hin4I HincII HindIII MmeI MscI MspA1I PfoI
SapI SfcI SmlI StuI StyI TaqII TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI FalI FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI ZraI