(Circular) (Six-base) MAPSORT of: pFN21AE0983.seq Check: 5970 from: 1 to: 6469 pFN21AE0983 6469 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2659 2659 Size: 6469 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3733 3812 1937 Size: 1796 79 4594 Fragments arranged by size: 4594 1796 79 Acc65I G'GTAC_C Cuts at: 3790 3790 Size: 6469 AclI AA'CG_TT Cuts at: 1563 2814 4596 4969 1563 Size: 1251 1782 373 3063 Fragments arranged by size: 3063 1782 1251 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 3032 4532 5916 6184 1632 Size: 833 567 1500 1384 268 1917 Fragments arranged by size: 1917 1500 1384 833 567 268 AfeI AGC'GCT Cuts at: 2738 2738 Size: 6469 AflII C'TTAA_G Cuts at: 820 1017 2783 820 Size: 197 1766 4506 Fragments arranged by size: 4506 1766 197 AflIII A'CryG_T Cuts at: 1203 5368 6345 1203 Size: 4165 977 1327 Fragments arranged by size: 4165 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 3073 3610 5198 3073 Size: 537 1588 4344 Fragments arranged by size: 4344 1588 537 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3767 1184 Size: 2583 3886 AlwNI CAG_nnn'CTG Cuts at: 4212 5784 4212 Size: 1572 4897 ApaLI G'TGCA_C Cuts at: 4526 5682 4526 Size: 1156 5313 ApoI r'AATT_y Cuts at: 1493 2276 2505 3778 4004 4156 6265 1493 Size: 783 229 1273 226 152 2109 1697 Fragments arranged by size: 2109 1697 1273 783 229 226 152 AseI AT'TA_AT Cuts at: 160 1032 2493 3333 5023 160 Size: 872 1461 840 1690 1606 Fragments arranged by size: 1690 1606 1461 872 840 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 2607 3794 1870 Size: 73 664 1187 4545 Fragments arranged by size: 4545 1187 664 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2930 2963 2930 Size: 33 6436 BamHI G'GATC_C Cuts at: 3799 4166 3799 Size: 367 6102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2407 2419 3790 5245 618 Size: 325 217 627 620 12 1371 1455 1842 Fragments arranged by size: 1842 1455 1371 627 620 325 217 12 BanII G_rGCy'C Cuts at: 1391 2064 3136 3788 1391 Size: 673 1072 652 4072 Fragments arranged by size: 4072 1072 673 652 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2191 3503 928 Size: 971 292 1312 3894 Fragments arranged by size: 3894 1312 971 292 BbvCI CC'TCA_GC Cuts at: 2757 2757 Size: 6469 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4660 4694 1903 Size: 34 2723 34 3678 Fragments arranged by size: 3678 2723 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4361 5571 4361 Size: 1210 5259 BclI T'GATC_A Cuts at: 1548 2686 1548 Size: 1138 5331 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5080 136 Size: 107 122 71 631 605 3408 1525 Fragments arranged by size: 3408 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 2876 3886 2876 Size: 1010 5459 Bme1580I G_kGCm'C Cuts at: 1790 4530 5686 1790 Size: 2740 1156 2573 Fragments arranged by size: 2740 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6469 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2947 3460 3526 Size: 416 53 83 399 24 1530 513 66 Cuts at: 3526 4200 5158 442 Size: 674 958 1753 Fragments arranged by size: 1753 1530 958 674 513 416 399 83 66 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2415 3120 3255 3323 3657 5129 1239 Size: 1176 705 135 68 334 1472 2579 Fragments arranged by size: 2579 1472 1176 705 334 135 68 Bpu10I CC'TnA_GC Cuts at: 2757 2757 Size: 6469 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3531 4594 5459 5757 3531 Size: 1063 865 298 4243 Fragments arranged by size: 4243 1063 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2604 3243 5132 882 Size: 617 1105 639 1889 2219 Fragments arranged by size: 2219 1889 1105 639 617 BsaAI yAC'GTr Cuts at: 493 1204 2707 3413 4260 493 Size: 711 1503 706 847 2702 Fragments arranged by size: 2702 1503 847 711 706 BsaBI GATnn'nnATC Cuts at: 6463 6463 Size: 6469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4276 4658 5293 275 Size: 53 83 186 936 2743 382 635 1451 Fragments arranged by size: 2743 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2766 2844 4902 5300 5574 5721 6132 2766 Size: 78 2058 398 274 147 411 3103 Fragments arranged by size: 3103 2058 411 398 274 147 78 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6244 Fragments arranged by size: 6244 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2774 1186 Size: 1588 4881 BseYI C'CCAG_C Cuts at: 2070 3541 5672 6168 2070 Size: 1471 2131 496 2371 Fragments arranged by size: 2371 2131 1471 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5896 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3919 4680 5708 1540 Size: 275 186 1918 761 1028 2301 Fragments arranged by size: 2301 1918 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3136 4530 4615 5686 3136 Size: 1394 85 1071 3919 Fragments arranged by size: 3919 1394 1071 85 BsmI GAATG_Cn' Cuts at: 3980 4073 3980 Size: 93 6376 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 3136 3788 4530 4615 5686 1391 Size: 399 274 1072 652 742 85 1071 2174 Fragments arranged by size: 2174 1072 1071 742 652 399 274 85 BspEI T'CCGG_A Cuts at: 2766 2844 6132 2766 Size: 78 3288 3103 Fragments arranged by size: 3288 3103 78 BspHI T'CATG_A Cuts at: 2109 4358 6088 2109 Size: 2249 1730 2490 Fragments arranged by size: 2490 2249 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2659 3824 844 Size: 1815 1165 3489 Fragments arranged by size: 3489 1815 1165 BsrBI CCG'CTC Cuts at: 1394 1823 4356 1394 Size: 429 2533 3507 Fragments arranged by size: 3507 2533 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2734 4964 5138 65 Size: 1183 1486 2230 174 1396 Fragments arranged by size: 2230 1486 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5113 5300 1700 Size: 110 3303 187 2869 Fragments arranged by size: 3303 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6469 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 2019 4529 5541 2019 Size: 2510 1012 2947 Fragments arranged by size: 2947 2510 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6469 BstBI TT'CG_AA Cuts at: 5273 5273 Size: 6469 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6469 BstXI CCAn_nnnn'nTGG Cuts at: 3380 3380 Size: 6469 BstYI r'GATC_y Cuts at: 1972 2763 3799 4166 4551 4568 6020 6106 6118 Size: 791 1036 367 385 17 1452 86 12 Cuts at: 6118 6464 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1036 791 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3734 3734 Size: 6469 BtgI C'CryG_G Cuts at: 513 1065 2004 3040 513 Size: 552 939 1036 3942 Fragments arranged by size: 3942 1036 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5730 Fragments arranged by size: 5730 683 56 BtsI GCAGTG_nn' Cuts at: 2466 3001 3751 3985 4779 4799 2466 Size: 535 750 234 794 20 4136 Fragments arranged by size: 4136 794 750 535 234 20 ClaI AT'CG_AT Cuts at: 1554 2626 1554 Size: 1072 5397 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6434 DraI TTT'AAA Cuts at: 3773 3773 Size: 6469 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5476 809 Size: 507 4160 1802 Fragments arranged by size: 4160 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2673 3916 4805 Size: 54 1425 50 275 18 843 1243 889 Cuts at: 4805 6434 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1243 889 843 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3233 4399 1353 Size: 1880 1166 3423 Fragments arranged by size: 3423 1880 1166 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2047 5056 5430 5576 387 Size: 1064 138 458 3009 374 146 1280 Fragments arranged by size: 3009 1280 1064 458 374 146 138 EcoICRI GAG'CTC Cuts at: 3134 3134 Size: 6469 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2415 2465 3032 3120 3255 3323 3657 Size: 393 783 50 567 88 135 68 334 Cuts at: 3657 4532 5129 5916 6184 1239 Size: 875 597 787 268 1524 Fragments arranged by size: 1524 875 787 783 597 567 393 334 268 135 88 68 50 EcoO109I rG'GnC_Cy Cuts at: 2589 3431 3520 2589 Size: 842 89 5538 Fragments arranged by size: 5538 842 89 EcoRI G'AATT_C Cuts at: 3778 4156 3778 Size: 378 6091 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3579 3579 Size: 6469 FspI TGC'GCA Cuts at: 2726 4975 2726 Size: 2249 4220 HaeII r_GCGC'y Cuts at: 1678 2740 5616 1678 Size: 1062 2876 2531 Fragments arranged by size: 2876 2531 1062 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3059 3091 3210 3242 3529 Size: 32 135 32 1487 32 119 32 287 Cuts at: 3529 3561 3596 3628 5110 5142 5184 5216 1373 Size: 32 35 32 1482 32 42 32 2626 Fragments arranged by size: 2626 1487 1482 287 135 119 42 35 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3813 4059 669 Size: 988 281 1875 246 3079 Fragments arranged by size: 3079 1875 988 281 246 HindIII A'AGCT_T Cuts at: 748 3695 748 Size: 2947 3522 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KpnI G_GTAC'C Cuts at: 3794 3794 Size: 6469 MfeI C'AATT_G Cuts at: 3171 4068 3171 Size: 897 5572 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2415 3661 5583 5767 1305 Size: 1110 1246 1922 184 2007 Fragments arranged by size: 2007 1922 1246 1110 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 10 Size: 54 1425 343 843 3804 Fragments arranged by size: 3804 1425 843 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 4427 4786 4945 518 Size: 648 942 2319 359 159 2042 Fragments arranged by size: 2319 2042 942 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 2266 2756 3081 3885 4562 5710 5955 Size: 471 189 490 325 804 677 1148 245 Cuts at: 5955 1606 Size: 2120 Fragments arranged by size: 2120 1148 804 677 490 471 325 245 189 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4978 Fragments arranged by size: 4978 939 552 NdeI CA'TA_TG Cuts at: 387 3179 387 Size: 2792 3677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 2827 3827 5372 1132 Size: 97 1598 1000 1545 2229 Fragments arranged by size: 2229 1598 1545 1000 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6469 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 2827 3655 3680 1108 Size: 1719 828 25 3897 Fragments arranged by size: 3897 1719 828 25 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4538 4570 6051 6083 4538 Size: 32 1481 32 4924 Fragments arranged by size: 4924 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3431 3520 3431 Size: 89 6380 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6469 PsiI TTA'TAA Cuts at: 4039 4039 Size: 6469 PstI C_TGCA'G Cuts at: 830 3821 830 Size: 2991 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 PvuII CAG'CTG Cuts at: 2266 2756 3081 2266 Size: 490 325 5654 Fragments arranged by size: 5654 490 325 SacI G_AGCT'C Cuts at: 3136 3136 Size: 6469 SalI G'TCGA_C Cuts at: 1936 3811 1936 Size: 1875 4594 SanDI GG'GwC_CC Cuts at: 3431 3520 3431 Size: 89 6380 SbfI CC_TGCA'GG Cuts at: 3821 3821 Size: 6469 ScaI AGT'ACT Cuts at: 3326 4717 6429 3326 Size: 1391 1712 3366 Fragments arranged by size: 3366 1712 1391 SfcI C'TryA_G Cuts at: 826 1045 3127 3160 3817 4952 5633 5824 826 Size: 219 2082 33 657 1135 681 191 1471 Fragments arranged by size: 2082 1471 1135 681 657 219 191 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 3796 1872 Size: 1924 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2607 2783 3546 4573 5474 5736 Size: 197 926 664 176 763 1027 901 262 Cuts at: 5736 820 Size: 1553 Fragments arranged by size: 1553 1027 926 901 763 664 262 197 176 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3827 1132 Size: 2695 3774 SspI AAT'ATT Cuts at: 5 52 4393 5 Size: 47 4341 2081 Fragments arranged by size: 4341 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6469 StyI C'CwwG_G Cuts at: 513 1065 2004 2403 3057 3339 3563 3908 513 Size: 552 939 399 654 282 224 345 3074 Fragments arranged by size: 3074 939 654 552 399 345 282 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2194 2425 4496 4513 4666 4851 5296 Size: 1255 614 231 2071 17 153 185 445 Cuts at: 5296 325 Size: 1498 Fragments arranged by size: 2071 1498 1255 614 445 231 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2149 3324 4715 Size: 274 80 33 51 1443 172 1175 1391 Cuts at: 4715 6427 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1391 1175 274 172 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6429 6461 6429 Size: 32 6437 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6469 XbaI T'CTAG_A Cuts at: 3805 3805 Size: 6469 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3177 3177 Size: 6469 XhoI C'TCGA_G Cuts at: 1943 2607 1943 Size: 664 5805 XmaI C'CCGG_G Cuts at: 1870 3794 1870 Size: 1924 4545 XmnI GAAnn'nnTTC Cuts at: 3024 4598 3024 Size: 1574 4895 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI