################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccIII AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BspDI BspEI BspHI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstPI CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NcoI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 180 CGCG CG^CG AclI 1540 AACGTT AA^CGTT AcsI 235 AAATTT [GA]^AATT[CT] AocI 1242 CCTGAGG CC^T[ACGT]AGG ApoI 235 AAATTT [GA]^AATT[CT] BamHI 60 GGATCC G^GATCC BfaI 44 CTAG C^TAG BsaAI 786 CACGTA [CT]AC^GT[GA] BseRI 73 GAGGAG GAGGAG(10/8) BsmI 1383 GCATTC GAATGC(1/-1) Bsp1407I 890 TGTACA T^GTACA BspLU11I 857 ACATGT A^CATGT BsrDI 297 CATTGC GCAATG(2/0) BsrGI 890 TGTACA T^GTACA BsrI 1443 ACTGG ACTGG(1/-1) Bst1107I 781 GTATAC GTA^TAC BstF5I 792 CATCC GGATG(2/0) BstUI 180 CGCG CG^CG BstZ17I 781 GTATAC GTA^TAC Bsu36I 1242 CCTGAGG CC^T[ACGT]AGG CfoI 624 GCGC GCG^C Eco81I 1242 CCTGAGG CC^T[ACGT]AGG FokI 792 CATCC GGATG(9/13) HaeII 623 GGCGCT [GA]GCGC^[CT] HgaI 1450 GACGC GACGC(5/10) HhaI 624 GCGC GCG^C HinP1I 624 GCGC G^CGC HindIII 127 AAGCTT A^AGCTT MaeI 44 CTAG C^TAG MvnI 180 CGCG CG^CG PaeR7I 1308 CTCGAG C^TCGAG PleI 102 GACTC GAGTC(4/5) Psp1406I 1540 AACGTT AA^CGTT SfcI 1343 CTACAG C^T[GA][CT]AG SmaI 913 CCCGGG CCC^GGG SphI 1188 GCATGC GCATG^C SspBI 890 TGTACA T^GTACA XhoI 1308 CTCGAG C^TCGAG XmaCI 913 CCCGGG C^CCGGG XmaI 913 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccI 781 GTATAC GT^[AC][GT]AC 1447 GTAGAC AfaI 789 GTAC GT^AC 891 GTAC AflIII 785 ACACGT A^C[GA][CT]GT 857 ACATGT AlwNI 430 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1237 CAGACCCTG AspHI 13 GAGCTC G[AT]GC[AT]^C 550 GAGCTC AvaI 913 CCCGGG C^[CT]CG[GA]G 1308 CTCGAG BalI 353 TGGCCA TGG^CCA 848 TGGCCA BanII 13 GAGCTC G[GA]GC[CT]^C 550 GAGCTC BclI 291 TGATCA T^GATCA 1392 TGATCA BmyI 13 GAGCTC G[AGT]GC[ACT]^C 550 GAGCTC BsiHKAI 13 GAGCTC G[AT]GC[AT]^C 550 GAGCTC BsoBI 913 CCCGGG C^[CT]CG[GA]G 1308 CTCGAG Bsp1286I 13 GAGCTC G[AGT]GC[ACT]^C 550 GAGCTC BstXI 356 CCAAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1112 CCAGCTTCCTGG BstYI 60 GGATCC [GA]^GATC[CT] 449 AGATCC EaeI 353 TGGCCA [CT]^GGCC[GA] 848 TGGCCA EarI 1157 CTCTTC CTCTTC(1/4) 414 GAAGAG EcoT14I 65 CCAAGG C^C[AT][AT]GG 1076 CCAAGG FbaI 291 TGATCA T^GATCA 1392 TGATCA Ksp632I 1157 CTCTTC CTCTTC(1/4) 414 GAAGAG MaeIII 81 GTCAC ^GT[ACGT]AC 100 GTGAC MflI 60 GGATCC [GA]^GATC[CT] 449 AGATCC MluNI 353 TGGCCA TGG^CCA 848 TGGCCA MscI 353 TGGCCA TGG^CCA 848 TGGCCA NaeI 3 GCCGGC GCC^GGC 973 GCCGGC NdeI 152 CATATG CA^TATG 502 CATATG NgoMIV 3 GCCGGC G^CCGGC 973 GCCGGC RsaI 789 GTAC GT^AC 891 GTAC SacI 13 GAGCTC GAGCT^C 550 GAGCTC StyI 65 CCAAGG C^C[AT][AT]GG 1076 CCAAGG TaqI 218 TCGA T^CGA 1309 TCGA Tsp45I 81 GTCAC ^GT[GC]AC 100 GTGAC Tsp509I 236 AATT ^AATT 1255 AATT TspRI 682 CACTG CA[GC]TG[ACGT][ACGT]^ 1425 CACTG TthHB8I 218 TCGA T^CGA 1309 TCGA XhoII 60 GGATCC [GA]^GATC[CT] 449 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BcnI BpmI BsaJI BsiYI BslI BsmAI BsrFI BssKI BstNI Cac8I Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI SmlI TfiI Tru9I TseI