(Circular) (Six-base) MAPSORT of: pFN21AE0968.seq Check: 3562 from: 1 to: 6265 pFN21AE0968 6265 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2787 3453 3608 1937 Size: 850 666 155 4594 Fragments arranged by size: 4594 850 666 155 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 3546 4392 4765 1563 Size: 1983 846 373 3063 Fragments arranged by size: 3063 1983 846 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2013 2303 2471 2593 4328 5712 5980 1632 Size: 381 290 168 122 1735 1384 268 1917 Fragments arranged by size: 1917 1735 1384 381 290 268 168 122 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 2790 2862 5164 6141 1203 Size: 1587 72 2302 977 1327 Fragments arranged by size: 2302 1587 1327 977 72 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 3095 1166 Size: 1929 4336 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2180 2212 2180 Size: 32 6233 AlwNI CAG_nnn'CTG Cuts at: 2440 3247 4008 5580 2440 Size: 807 761 1572 3125 Fragments arranged by size: 3125 1572 807 761 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 2240 3574 3800 3952 6061 1493 Size: 747 1334 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 747 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2918 3313 3590 1870 Size: 73 975 395 277 4545 Fragments arranged by size: 4545 975 395 277 73 BamHI G'GATC_C Cuts at: 2065 3595 3962 2065 Size: 1530 367 4368 Fragments arranged by size: 4368 1530 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3586 5041 618 Size: 325 217 627 1799 1455 1842 Fragments arranged by size: 1842 1799 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2559 3584 1391 Size: 631 537 1025 4072 Fragments arranged by size: 4072 1025 631 537 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4456 4490 1903 Size: 34 50 34 2435 34 3678 Fragments arranged by size: 3678 2435 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 2296 3397 1548 Size: 748 1101 4416 Fragments arranged by size: 4416 1101 748 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4876 136 Size: 107 122 71 631 605 337 2867 1525 Fragments arranged by size: 2867 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 4326 5482 1790 Size: 2536 1156 2573 Fragments arranged by size: 2573 2536 1156 BmgBI CAC'GTC Cuts at: 1318 2851 1318 Size: 1533 4732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3996 4954 442 Size: 416 53 83 399 24 2579 958 1753 Fragments arranged by size: 2579 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2983 3398 4925 1239 Size: 1106 638 415 1527 2579 Fragments arranged by size: 2579 1527 1106 638 415 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2006 2681 3173 3206 3518 4390 5255 5553 2006 Size: 675 492 33 312 872 865 298 2718 Fragments arranged by size: 2718 872 865 675 492 312 298 33 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2793 4056 493 Size: 711 1589 1263 2702 Fragments arranged by size: 2702 1589 1263 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 1178 Size: 30 165 30 777 30 5233 Fragments arranged by size: 5233 777 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 1186 Size: 907 5358 BseYI C'CCAG_C Cuts at: 2962 5468 5964 2962 Size: 2506 496 3263 Fragments arranged by size: 3263 2506 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5692 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 2559 4326 4411 5482 2022 Size: 537 1767 85 1071 2805 Fragments arranged by size: 2805 1767 1071 537 85 BsmI GAATG_Cn' Cuts at: 3388 3776 3869 3388 Size: 388 93 5784 Fragments arranged by size: 5784 388 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2559 3584 4326 4411 5482 1391 Size: 399 232 537 1025 742 85 1071 2174 Fragments arranged by size: 2174 1071 1025 742 537 399 232 85 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3620 844 Size: 2776 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4152 1394 Size: 429 2329 3507 Fragments arranged by size: 3507 2329 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2301 4760 4934 65 Size: 1183 1053 2459 174 1396 Fragments arranged by size: 2459 1396 1183 1053 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 2805 2978 4909 5096 1700 Size: 110 198 797 173 1931 187 2869 Fragments arranged by size: 2869 1931 797 198 187 173 110 BsrGI T'GTAC_A Cuts at: 96 2895 96 Size: 2799 3466 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6265 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstXI CCAn_nnnn'nTGG Cuts at: 2368 3124 2368 Size: 756 5509 BstYI r'GATC_y Cuts at: 1972 2065 2454 3595 3962 4347 4364 5816 5902 Size: 93 389 1141 367 385 17 1452 86 Cuts at: 5902 5914 6260 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1141 389 385 367 346 93 86 17 12 BstZ17I GTA'TAC Cuts at: 2788 2788 Size: 6265 Bsu36I CC'TnA_GG Cuts at: 3248 3248 Size: 6265 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5713 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 3781 4575 4595 3781 Size: 794 20 5451 Fragments arranged by size: 5451 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 2853 3712 Size: 54 1425 50 275 18 528 495 859 Cuts at: 3712 4601 6230 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 859 528 495 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2414 3168 4195 1353 Size: 1061 754 1027 3423 Fragments arranged by size: 3423 1061 1027 754 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2903 3503 4852 5226 5372 387 Size: 1064 138 1314 600 1349 374 146 1280 Fragments arranged by size: 1349 1314 1280 1064 600 374 146 138 EcoICRI GAG'CTC Cuts at: 2020 2557 2020 Size: 537 5728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2013 2303 2345 2471 2593 2983 3398 Size: 393 381 290 42 126 122 390 415 Cuts at: 3398 4328 4925 5712 5980 1239 Size: 930 597 787 268 1524 Fragments arranged by size: 1524 930 787 597 415 393 390 381 290 268 126 122 42 EcoO109I rG'GnC_Cy Cuts at: 2125 2287 2343 2437 3085 3184 3237 2125 Size: 162 56 94 648 99 53 5153 Fragments arranged by size: 5153 648 162 99 94 56 53 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2362 2362 Size: 6265 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2632 5412 1678 Size: 954 2780 2531 Fragments arranged by size: 2780 2531 954 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 2744 2776 4906 Size: 32 135 32 497 32 643 32 2130 Cuts at: 4906 4938 4980 5012 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2130 643 497 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 2132 748 Size: 1384 4881 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5379 5563 1305 Size: 4074 184 2007 Fragments arranged by size: 4074 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 2855 10 Size: 54 1425 343 528 495 3420 Fragments arranged by size: 3420 1425 528 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2861 2957 3095 3393 4223 4582 4741 Size: 648 1695 96 138 298 830 359 159 Cuts at: 4741 518 Size: 2042 Fragments arranged by size: 2042 1695 830 648 359 298 159 138 96 MspA1I CmG'CkG Cuts at: 1606 2117 2149 2941 3439 3479 3681 4358 5506 Size: 511 32 792 498 40 202 677 1148 Cuts at: 5506 5751 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 792 677 511 498 245 202 40 32 NaeI GCC'GGC Cuts at: 2010 2980 2010 Size: 970 5295 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5713 NdeI CA'TA_TG Cuts at: 387 2158 2508 387 Size: 1771 350 4144 Fragments arranged by size: 4144 1771 350 NgoMIV G'CCGG_C Cuts at: 2008 2978 2008 Size: 970 5295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2866 3197 3383 3623 5168 1132 Size: 97 1637 331 186 240 1545 2229 Fragments arranged by size: 2229 1637 1545 331 240 186 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 2862 5164 2862 Size: 2302 3963 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6265 PfoI T'CCnGG_A Cuts at: 2457 2999 3123 2457 Size: 542 124 5599 Fragments arranged by size: 5599 542 124 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2180 2212 4334 4366 5847 5879 2180 Size: 32 2122 32 1481 32 2566 Fragments arranged by size: 2566 2122 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2287 2343 3085 2287 Size: 56 742 5467 Fragments arranged by size: 5467 742 56 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspXI vC'TCGA_Gb Cuts at: 3313 3313 Size: 6265 PstI C_TGCA'G Cuts at: 830 3617 830 Size: 2787 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2149 2941 3439 3479 2149 Size: 792 498 40 4935 Fragments arranged by size: 4935 792 498 40 SacI G_AGCT'C Cuts at: 2022 2559 2022 Size: 537 5728 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SfcI C'TryA_G Cuts at: 826 1045 3348 3613 4748 5429 5620 826 Size: 219 2303 265 1135 681 191 1471 Fragments arranged by size: 2303 1471 1135 681 265 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 2920 3592 1872 Size: 1048 672 4545 Fragments arranged by size: 4545 1048 672 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 2696 3188 3221 3313 3533 Size: 197 926 78 675 492 33 92 220 Cuts at: 3533 4369 5270 5532 820 Size: 836 901 262 1553 Fragments arranged by size: 1553 926 901 836 675 492 262 220 197 92 78 33 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3197 3623 1132 Size: 2065 426 3774 Fragments arranged by size: 3774 2065 426 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6265 StyI C'CwwG_G Cuts at: 513 1065 2070 3081 3704 513 Size: 552 1005 1011 623 3074 Fragments arranged by size: 3074 1011 1005 623 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4292 4309 4462 4647 5092 325 Size: 1255 2712 17 153 185 445 1498 Fragments arranged by size: 2712 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2895 4511 6223 Size: 274 80 33 51 1443 918 1616 1712 Cuts at: 6223 96 Size: 138 Fragments arranged by size: 1712 1616 1443 918 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 3313 1943 Size: 1370 4895 XmaI C'CCGG_G Cuts at: 1870 2918 3590 1870 Size: 1048 672 4545 Fragments arranged by size: 4545 1048 672 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI