(Circular) (Six-base) MAPSORT of: pFN21AE0948.seq Check: 5257 from: 1 to: 5983 pFN21AE0948 5983 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2106 3326 1937 Size: 169 1220 4594 Fragments arranged by size: 4594 1220 169 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 2819 3157 4046 5430 5698 1632 Size: 767 420 338 889 1384 268 1917 Fragments arranged by size: 1917 1384 889 767 420 338 268 AfeI AGC'GCT Cuts at: 2870 2870 Size: 5983 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 4882 5859 1203 Size: 3679 977 1327 Fragments arranged by size: 3679 1327 977 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 2599 4712 2599 Size: 2113 3870 AleI CACnn'nnGTG Cuts at: 1166 2528 1166 Size: 1362 4621 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5983 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2397 2429 2397 Size: 32 5951 AlwNI CAG_nnn'CTG Cuts at: 3076 3726 5298 3076 Size: 650 1572 3761 Fragments arranged by size: 3761 1572 650 ApaI G_GGCC'C Cuts at: 2954 2954 Size: 5983 ApaLI G'TGCA_C Cuts at: 3221 4040 5196 3221 Size: 819 1156 4008 Fragments arranged by size: 4008 1156 819 ApoI r'AATT_y Cuts at: 1493 3292 3518 3670 5779 1493 Size: 1799 226 152 2109 1697 Fragments arranged by size: 2109 1799 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 2040 3308 1870 Size: 73 97 1268 4545 Fragments arranged by size: 4545 1268 97 73 BamHI G'GATC_C Cuts at: 3313 3680 3313 Size: 367 5616 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2534 2551 3304 4759 618 Size: 325 217 627 747 17 753 1455 1842 Fragments arranged by size: 1842 1455 753 747 627 325 217 17 BanII G_rGCy'C Cuts at: 1391 2061 2093 2796 2853 2954 3048 3111 3302 Size: 670 32 703 57 101 94 63 191 Cuts at: 3302 1391 Size: 4072 Fragments arranged by size: 4072 703 670 191 101 94 63 57 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2837 3091 928 Size: 971 938 254 3820 Fragments arranged by size: 3820 971 938 254 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4174 4208 1903 Size: 34 2237 34 3678 Fragments arranged by size: 3678 2237 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4594 136 Size: 107 122 71 631 605 2922 1525 Fragments arranged by size: 2922 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5978 5978 Size: 5983 BlpI GC'TnA_GC Cuts at: 2308 3400 2308 Size: 1092 4891 Bme1580I G_kGCm'C Cuts at: 1790 2537 2954 3225 4044 5200 1790 Size: 747 417 271 819 1156 2573 Fragments arranged by size: 2573 1156 819 747 417 271 BmgBI CAC'GTC Cuts at: 1318 2464 1318 Size: 1146 4837 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3064 3714 4672 Size: 416 53 83 399 24 1647 650 958 Cuts at: 4672 442 Size: 1753 Fragments arranged by size: 1753 1647 958 650 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2920 2920 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2360 2729 3173 3182 4643 1239 Size: 944 177 369 444 9 1461 2579 Fragments arranged by size: 2579 1461 944 444 369 177 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4108 4973 5271 4108 Size: 865 298 4820 Fragments arranged by size: 4820 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4646 882 Size: 617 3147 2219 Fragments arranged by size: 3147 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3774 493 Size: 711 2570 2702 Fragments arranged by size: 2702 2570 711 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3790 4172 4807 275 Size: 53 83 186 936 2257 382 635 1451 Fragments arranged by size: 2257 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4416 4814 5088 5235 5646 4416 Size: 398 274 147 411 4753 Fragments arranged by size: 4753 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 3130 3160 1178 Size: 30 165 30 834 30 863 30 4001 Fragments arranged by size: 4001 863 834 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2180 2357 2909 3170 3200 1186 Size: 883 111 177 552 261 30 3969 Fragments arranged by size: 3969 883 552 261 177 111 30 BseYI C'CCAG_C Cuts at: 5186 5682 5186 Size: 496 5487 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 3005 3260 1163 Size: 573 645 624 255 3886 Fragments arranged by size: 3886 645 624 573 255 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2077 2860 2984 3433 4194 5222 Size: 275 186 76 783 124 449 761 1028 Cuts at: 5222 1540 Size: 2301 Fragments arranged by size: 2301 1028 783 761 449 275 186 124 76 BsiHKAI G_wGCw'C Cuts at: 2061 2270 2796 2853 3111 3225 4044 4129 5200 Size: 209 526 57 258 114 819 85 1071 Cuts at: 5200 2061 Size: 2844 Fragments arranged by size: 2844 1071 819 526 258 209 114 85 57 BsmI GAATG_Cn' Cuts at: 3494 3587 3494 Size: 93 5890 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2093 2270 2537 2796 2853 2954 Size: 399 271 32 177 267 259 57 101 Cuts at: 2954 3048 3111 3225 3302 4044 4129 5200 1391 Size: 94 63 114 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 271 267 259 177 114 101 94 85 77 63 57 32 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 3872 5602 3872 Size: 1730 4253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3338 844 Size: 2494 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3132 3870 1394 Size: 429 1309 738 3507 Fragments arranged by size: 3507 1309 738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2352 4478 4652 65 Size: 1183 1104 2126 174 1396 Fragments arranged by size: 2126 1396 1183 1104 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4627 4814 1700 Size: 110 2817 187 2869 Fragments arranged by size: 2869 2817 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 4043 5055 4043 Size: 1012 4971 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5983 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstXI CCAn_nnnn'nTGG Cuts at: 2626 2626 Size: 5983 BstYI r'GATC_y Cuts at: 1972 2412 3313 3680 4065 4082 5534 5620 5632 Size: 440 901 367 385 17 1452 86 12 Cuts at: 5632 5978 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 901 440 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2052 2556 2052 Size: 504 5479 BtgI C'CryG_G Cuts at: 513 1065 2004 2781 513 Size: 552 939 777 3715 Fragments arranged by size: 3715 939 777 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5244 Fragments arranged by size: 5244 683 56 BtsI GCAGTG_nn' Cuts at: 3499 4293 4313 3499 Size: 794 20 5169 Fragments arranged by size: 5169 794 20 ClaI AT'CG_AT Cuts at: 1554 2118 1554 Size: 564 5419 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2118 2153 529 Size: 35 1554 35 4359 Fragments arranged by size: 4359 1554 35 35 DraI TTT'AAA Cuts at: 3287 3287 Size: 5983 DraIII CAC_nnn'GTG Cuts at: 3229 3229 Size: 5983 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2628 2784 Size: 54 1425 50 275 18 312 486 156 Cuts at: 2784 2981 3180 3430 4319 5948 8 Size: 197 199 250 889 1629 43 Fragments arranged by size: 1629 1425 889 486 312 275 250 199 197 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2981 3430 1537 Size: 275 1169 449 4090 Fragments arranged by size: 4090 1169 449 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 3913 1353 Size: 713 1847 3423 Fragments arranged by size: 3423 1847 713 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2489 2948 4570 4944 5090 387 Size: 1064 138 900 459 1622 374 146 1280 Fragments arranged by size: 1622 1280 1064 900 459 374 146 138 EcoICRI GAG'CTC Cuts at: 2059 2794 2851 3109 2059 Size: 735 57 258 4933 Fragments arranged by size: 4933 735 258 57 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2360 2399 2729 2819 3157 3173 Size: 393 551 177 39 330 90 338 16 Cuts at: 3173 3182 4046 4643 5430 5698 1239 Size: 9 864 597 787 268 1524 Fragments arranged by size: 1524 864 787 597 551 393 338 330 268 177 90 39 16 9 EcoNI CCTnn'n_nnAGG Cuts at: 2165 3182 2165 Size: 1017 4966 EcoO109I rG'GnC_Cy Cuts at: 2333 2383 2950 2951 3066 2333 Size: 50 567 1 115 5250 Fragments arranged by size: 5250 567 115 50 1 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2854 2854 Size: 5983 FspI TGC'GCA Cuts at: 4489 4489 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 2872 5130 1678 Size: 1194 2258 2531 Fragments arranged by size: 2531 2258 1194 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 4624 4656 4698 Size: 32 135 32 1013 32 2007 32 42 Cuts at: 4698 4730 1373 Size: 32 2626 Fragments arranged by size: 2626 2007 1013 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 2999 748 Size: 2251 3732 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 3582 3582 Size: 5983 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5097 5281 1305 Size: 3792 184 2007 Fragments arranged by size: 3792 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 2630 10 Size: 54 1425 343 312 486 3363 Fragments arranged by size: 3363 1425 486 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 3941 4300 4459 518 Size: 648 1362 1413 359 159 2042 Fragments arranged by size: 2042 1413 1362 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3399 4076 5224 5469 1606 Size: 1793 677 1148 245 2120 Fragments arranged by size: 2120 1793 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4492 Fragments arranged by size: 4492 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 3341 4886 1132 Size: 97 2112 1545 2229 Fragments arranged by size: 2229 2112 1545 97 PasI CC'CwG_GG Cuts at: 1348 2605 1348 Size: 1257 4726 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 2277 1108 Size: 1169 4814 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4052 4084 5565 5597 4052 Size: 32 1481 32 4438 Fragments arranged by size: 4438 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2333 3066 2333 Size: 733 5250 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PspOMI G'GGCC_C Cuts at: 2950 2950 Size: 5983 PstI C_TGCA'G Cuts at: 830 3335 830 Size: 2505 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 SacI G_AGCT'C Cuts at: 2061 2796 2853 3111 2061 Size: 735 57 258 4933 Fragments arranged by size: 4933 735 258 57 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SapI GCTCTTCn'nnn_ Cuts at: 2066 2066 Size: 5983 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SexAI A'CCwGG_T Cuts at: 2329 3225 2329 Size: 896 5087 SfcI C'TryA_G Cuts at: 826 1045 3331 4466 5147 5338 826 Size: 219 2286 1135 681 191 1471 Fragments arranged by size: 2286 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 3310 1872 Size: 1438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2040 4087 4988 5250 820 Size: 197 926 97 2047 901 262 1553 Fragments arranged by size: 2047 1553 926 901 262 197 97 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 3341 1132 Size: 2209 3774 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 2750 1517 Size: 1233 4750 StyI C'CwwG_G Cuts at: 513 1065 2004 2631 2787 3422 513 Size: 552 939 627 156 635 3074 Fragments arranged by size: 3074 939 635 627 552 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2716 3134 4010 4027 4180 4365 4810 Size: 1255 1136 418 876 17 153 185 445 Cuts at: 4810 325 Size: 1498 Fragments arranged by size: 1498 1255 1136 876 445 418 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4229 5941 96 Size: 274 80 33 51 1443 2252 1712 138 Fragments arranged by size: 2252 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3130 3162 3257 3289 5943 5975 3130 Size: 32 95 32 2654 32 3138 Fragments arranged by size: 3138 2654 95 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XhoI C'TCGA_G Cuts at: 1943 2040 1943 Size: 97 5886 XmaI C'CCGG_G Cuts at: 1870 3308 1870 Size: 1438 4545 XmnI GAAnn'nnTTC Cuts at: 2525 3170 4112 2525 Size: 645 942 4396 Fragments arranged by size: 4396 942 645 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI