Restriction map for pFN21AE0945
 (Circular) (Six-base) MAPSORT of: pFN21AE0945.seq  Check: 2818  from: 1  to: 5755



pFN21AE0945  5755 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:38  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3565     278 
   Size:         53      83     186     936    2029    2468

  Fragments arranged by size: 

                2468    2029     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3098    1937 
   Size:       1161    4594
 
Acc65I G'GTAC_C

Cuts at:   3076    3076 
   Size:       5755
 
AclI AA'CG_TT

Cuts at:   1563    3882    4255    1563 
   Size:       2319     373    3063

  Fragments arranged by size: 

                3063    2319     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2167    2276    2650    3818    5202    5470    1632 
   Size:        535     109     374    1168    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1168     535     374     268     109
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5558
 
AflIII A'CryG_T

Cuts at:   1203    2401    2523    4654    5631    1203 
   Size:       1198     122    2131     977    1327

  Fragments arranged by size: 

                2131    1327    1198     977     122
 
AgeI A'CCGG_T

Cuts at:   4586    4586 
   Size:       5755
 
AhdI GACnn_n'nnGTC

Cuts at:   4484    4484 
   Size:       5755
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5755
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2334    1184 
   Size:       1150    4605
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2699    2731    2699 
   Size:         32    5723
 
AlwNI CAG_nnn'CTG

Cuts at:   2380    2650    3498    5070    2380 
   Size:        270     848    1572    3065

  Fragments arranged by size: 

                3065    1572     848     270
 
ApaLI G'TGCA_C

Cuts at:   3812    4968    3812 
   Size:       1156    4599
 
ApoI r'AATT_y

Cuts at:   1493    3064    3290    3442    5551    1493 
   Size:       1571     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1571     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2571    4309     160 
   Size:        872    1539    1738    1606

  Fragments arranged by size: 

                1738    1606    1539     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5755
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2046    3080    1870 
   Size:         73     103    1034    4545

  Fragments arranged by size: 

                4545    1034     103      73
 
BamHI G'GATC_C

Cuts at:   3085    3452    3085 
   Size:        367    5388
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2053    3076    4531     618 
   Size:        325     217     627     266    1023    1455    1842

  Fragments arranged by size: 

                1842    1455    1023     627     325     266     217
 
BanII G_rGCy'C

Cuts at:   1391    3074    1391 
   Size:       1683    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2639     928 
   Size:        971     740    4044

  Fragments arranged by size: 

                4044     971     740
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3946    3980    1903 
   Size:         34    2009      34    3678

  Fragments arranged by size: 

                3678    2009      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3004    3647    4857    3004 
   Size:        643    1210    3902

  Fragments arranged by size: 

                3902    1210     643
 
BclI T'GATC_A

Cuts at:   1548    2079    1548 
   Size:        531    5224
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2595    4366     136 
   Size:        107     122      71     631     605     923    1771    1525

  Fragments arranged by size: 

                1771    1525     923     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5750    5750 
   Size:       5755
 
BlpI GC'TnA_GC

Cuts at:   3172    3172 
   Size:       5755
 
Bme1580I G_kGCm'C

Cuts at:   1790    2600    2889    3816    4972    1790 
   Size:        810     289     927    1156    2573

  Fragments arranged by size: 

                2573    1156     927     810     289
 
BmgBI CAC'GTC

Cuts at:   1318    2800    1318 
   Size:       1482    4273
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2638    3486    4444 
   Size:        416      53      83     399      24    1221     848     958

Cuts at:   4444     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1221     958     848     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5755
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2970    2970 
   Size:       5755
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2363    2561    2754    2854    3008    4415    1239 
   Size:       1124     198     193     100     154    1407    2579

  Fragments arranged by size: 

                2579    1407    1124     198     193     154     100
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2494    3880    4745    5043    2494 
   Size:       1386     865     298    3206

  Fragments arranged by size: 

                3206    1386     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2984    4418     882 
   Size:        617    1485    1434    2219

  Fragments arranged by size: 

                2219    1485    1434     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2526    3546     493 
   Size:        711    1322    1020    2702

  Fragments arranged by size: 

                2702    1322    1020     711
 
BsaBI GATnn'nnATC

Cuts at:   5749    5749 
   Size:       5755
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2691    3562    3944    4579 
   Size:         53      83     186     936    1158     871     382     635

Cuts at:   4579     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1158     936     871     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4188    4586    4860    5007    5418    4188 
   Size:        398     274     147     411    4525

  Fragments arranged by size: 

                4525     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2699    2729    2784    2814    1178 
   Size:         30     165      30    1296      30      55      30    4119

  Fragments arranged by size: 

                4119    1296     165      55      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2127    2243    1186 
   Size:        941     116    4698

  Fragments arranged by size: 

                4698     941     116
 
BseYI C'CCAG_C

Cuts at:   4958    5454    4958 
   Size:        496    5259
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2564    1163 
   Size:        573     828    4354

  Fragments arranged by size: 

                4354     828     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3205    3966    4994    1540 
   Size:        275     186    1204     761    1028    2301

  Fragments arranged by size: 

                2301    1204    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2313    3816    3901    4972    2313 
   Size:       1503      85    1071    3096

  Fragments arranged by size: 

                3096    1503    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3266    3359    3266 
   Size:         93    5662
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2313    2600    2889    3074    3816    3901    4972 
   Size:        399     523     287     289     185     742      85    1071

Cuts at:   4972    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     523     399     289     287     185
                  85
 
BspEI T'CCGG_A

Cuts at:   5418    5418 
   Size:       5755
 
BspHI T'CATG_A

Cuts at:   3644    5374    3644 
   Size:       1730    4025
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2586    2634    3110     844 
   Size:       1742      48     476    3489

  Fragments arranged by size: 

                3489    1742     476      48
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3642    1394 
   Size:        429    1819    3507

  Fragments arranged by size: 

                3507    1819     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4250    4424      65 
   Size:       1183    3002     174    1396

  Fragments arranged by size: 

                3002    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4399    4586    1700 
   Size:        110    2589     187    2869

  Fragments arranged by size: 

                2869    2589     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5755
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5755
 
BssSI C'ACGA_G

Cuts at:   3815    4827    3815 
   Size:       1012    4743
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5755
 
BstBI TT'CG_AA

Cuts at:   4559    4559 
   Size:       5755
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5755
 
BstYI r'GATC_y

Cuts at:   1972    2856    3085    3452    3837    3854    5306    5392    5404 
   Size:        884     229     367     385      17    1452      86      12

Cuts at:   5404    5750    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     884     385     367     346     229      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2365    2550     513 
   Size:        552     939     361     185    3718

  Fragments arranged by size: 

                3718     939     552     361     185
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2116     487 
   Size:        683      56     890    4126

  Fragments arranged by size: 

                4126     890     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2970    3271    4065    4085    2970 
   Size:        301     794      20    4640

  Fragments arranged by size: 

                4640     794     301      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5755
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5720
 
DraI TTT'AAA

Cuts at:   3059    3059 
   Size:       5755
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4762     809 
   Size:        507    3446    1802

  Fragments arranged by size: 

                3446    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2547    2737    3202 
   Size:         54    1425      50     275      18     717     190     465

Cuts at:   3202    4091    5720       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     717     465     275     190      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3202    1537 
   Size:        275    1390    4090

  Fragments arranged by size: 

                4090    1390     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3685    1353 
   Size:       2332    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4342    4716    4862     387 
   Size:       1064     138    2753     374     146    1280

  Fragments arranged by size: 

                2753    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2167    2276    2363    2561    2650    2754    2854 
   Size:        393     535     109      87     198      89     104     100

Cuts at:   2854    3008    3818    4415    5202    5470    1239 
   Size:        154     810     597     787     268    1524

  Fragments arranged by size: 

                1524     810     787     597     535     393     268     198
                 154     109     104     100      89      87
 
EcoRI G'AATT_C

Cuts at:   3064    3442    3064 
   Size:        378    5377
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2305    2305 
   Size:       5755
 
FspI TGC'GCA

Cuts at:   4261    4261 
   Size:       5755
 
HaeII r_GCGC'y

Cuts at:   1678    4902    1678 
   Size:       3224    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2083    2115    4396    4428    4470 
   Size:         32     135      32     511      32    2281      32      42

Cuts at:   4470    4502    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2281     511     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3099    3345     669 
   Size:        988     281    1161     246    3079

  Fragments arranged by size: 

                3079    1161     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5755
 
HpaI GTT'AAC

Cuts at:   3345    3345 
   Size:       5755
 
KpnI G_GTAC'C

Cuts at:   3080    3080 
   Size:       5755
 
MfeI C'AATT_G

Cuts at:   3354    3354 
   Size:       5755
 
MluI A'CGCG_T

Cuts at:   5631    5631 
   Size:       5755
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2643    4869    5053    1305 
   Size:       1338    2226     184    2007

  Fragments arranged by size: 

                2226    2007    1338     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2549      10 
   Size:         54    1425     343     717    3216

  Fragments arranged by size: 

                3216    1425     717     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3713    4072    4231     518 
   Size:        648    2547     359     159    2042

  Fragments arranged by size: 

                2547    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2063    2254    3171    3848    4996    5241    1606 
   Size:        457     191     917     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     917     677     457     245     191
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2365    2550     513 
   Size:        552     939     361     185    3718

  Fragments arranged by size: 

                3718     939     552     361     185
 
NdeI CA'TA_TG

Cuts at:    387    2830     387 
   Size:       2443    3312
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5755
 
NotI GC'GGCC_GC

Cuts at:   3202    3202 
   Size:       5755
 
NspI r_CATG'y

Cuts at:   1132    1229    2405    2943    3113    4658    1132 
   Size:         97    1176     538     170    1545    2229

  Fragments arranged by size: 

                2229    1545    1176     538     170      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5755
 
PciI A'CATG_T

Cuts at:   2401    4654    2401 
   Size:       2253    3502
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2066    1108 
   Size:        958    4797
 
PfoI T'CCnGG_A

Cuts at:   2717    2717 
   Size:       5755
 
PmeI GTTT'AAAC

Cuts at:   3059    3059 
   Size:       5755
 
PmlI CAC'GTG

Cuts at:   2526    2526 
   Size:       5755
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2699    2731    3824    3856    5337    5369    2699 
   Size:         32    1093      32    1481      32    3085

  Fragments arranged by size: 

                3085    1481    1093      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5755
 
PsiI TTA'TAA

Cuts at:   3325    3325 
   Size:       5755
 
PstI C_TGCA'G

Cuts at:    830    2376    3107     830 
   Size:       1546     731    3478

  Fragments arranged by size: 

                3478    1546     731
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5755
 
PvuII CAG'CTG

Cuts at:   2063    2254    2063 
   Size:        191    5564
 
SalI G'TCGA_C

Cuts at:   1936    3097    1936 
   Size:       1161    4594
 
SbfI CC_TGCA'GG

Cuts at:   3107    3107 
   Size:       5755
 
ScaI AGT'ACT

Cuts at:   4003    5715    4003 
   Size:       1712    4043
 
SfcI C'TryA_G

Cuts at:    826    1045    2372    3103    4238    4919    5110     826 
   Size:        219    1327     731    1135     681     191    1471

  Fragments arranged by size: 

                1471    1327    1135     731     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5755
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5755
 
SmaI CCC'GGG

Cuts at:   1872    3082    1872 
   Size:       1210    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2473    3859    4760    5022     820 
   Size:        197     926     530    1386     901     262    1553

  Fragments arranged by size: 

                1553    1386     926     901     530     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3546     493 
   Size:       3053    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5755
 
SphI G_CATG'C

Cuts at:   1132    2943    3113    1132 
   Size:       1811     170    3774

  Fragments arranged by size: 

                3774    1811     170
 
SspI AAT'ATT

Cuts at:      5      52    3679       5 
   Size:         47    3627    2081

  Fragments arranged by size: 

                3627    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2037    1517 
   Size:        520    5235
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2213    2365    2392    2550    3194     513 
   Size:        552     939     209     152      27     158     644    3074

  Fragments arranged by size: 

                3074     939     644     552     209     158     152      27
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3782    3799    3952    4137    4582     325 
   Size:       1255    2202      17     153     185     445    1498

  Fragments arranged by size: 

                2202    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4001    5713      96 
   Size:        274      80      33      51    1443    2024    1712     138

  Fragments arranged by size: 

                2024    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2152    2184    2784    2816    5715    5747    2152 
   Size:         32     600      32    2899      32    2160

  Fragments arranged by size: 

                2899    2160     600      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2558    2638    1316 
   Size:       1242      80    4433

  Fragments arranged by size: 

                4433    1242      80
 
XbaI T'CTAG_A

Cuts at:   3091    3091 
   Size:       5755
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2778    2878    2778 
   Size:        100    5655
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5755
 
XmaI C'CCGG_G

Cuts at:   1870    3080    1870 
   Size:       1210    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3884    3884 
   Size:       5755
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3563     276 
   Size:         53      83     186     936    2029    2468

  Fragments arranged by size: 

                2468    2029     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI    PpuMI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI
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