(Circular) (Six-base) MAPSORT of: pFN21AE0945.seq Check: 2818 from: 1 to: 5755 pFN21AE0945 5755 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2167 2276 2650 3818 5202 5470 1632 Size: 535 109 374 1168 1384 268 1917 Fragments arranged by size: 1917 1384 1168 535 374 268 109 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 2401 2523 4654 5631 1203 Size: 1198 122 2131 977 1327 Fragments arranged by size: 2131 1327 1198 977 122 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2334 1184 Size: 1150 4605 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2699 2731 2699 Size: 32 5723 AlwNI CAG_nnn'CTG Cuts at: 2380 2650 3498 5070 2380 Size: 270 848 1572 3065 Fragments arranged by size: 3065 1572 848 270 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 3064 3290 3442 5551 1493 Size: 1571 226 152 2109 1697 Fragments arranged by size: 2109 1697 1571 226 152 AseI AT'TA_AT Cuts at: 160 1032 2571 4309 160 Size: 872 1539 1738 1606 Fragments arranged by size: 1738 1606 1539 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2046 3080 1870 Size: 73 103 1034 4545 Fragments arranged by size: 4545 1034 103 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 3076 4531 618 Size: 325 217 627 266 1023 1455 1842 Fragments arranged by size: 1842 1455 1023 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 3074 1391 Size: 1683 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2639 928 Size: 971 740 4044 Fragments arranged by size: 4044 971 740 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3946 3980 1903 Size: 34 2009 34 3678 Fragments arranged by size: 3678 2009 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3004 3647 4857 3004 Size: 643 1210 3902 Fragments arranged by size: 3902 1210 643 BclI T'GATC_A Cuts at: 1548 2079 1548 Size: 531 5224 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2595 4366 136 Size: 107 122 71 631 605 923 1771 1525 Fragments arranged by size: 1771 1525 923 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 2600 2889 3816 4972 1790 Size: 810 289 927 1156 2573 Fragments arranged by size: 2573 1156 927 810 289 BmgBI CAC'GTC Cuts at: 1318 2800 1318 Size: 1482 4273 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2638 3486 4444 Size: 416 53 83 399 24 1221 848 958 Cuts at: 4444 442 Size: 1753 Fragments arranged by size: 1753 1221 958 848 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2970 2970 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2561 2754 2854 3008 4415 1239 Size: 1124 198 193 100 154 1407 2579 Fragments arranged by size: 2579 1407 1124 198 193 154 100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2494 3880 4745 5043 2494 Size: 1386 865 298 3206 Fragments arranged by size: 3206 1386 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2984 4418 882 Size: 617 1485 1434 2219 Fragments arranged by size: 2219 1485 1434 617 BsaAI yAC'GTr Cuts at: 493 1204 2526 3546 493 Size: 711 1322 1020 2702 Fragments arranged by size: 2702 1322 1020 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2691 3562 3944 4579 Size: 53 83 186 936 1158 871 382 635 Cuts at: 4579 275 Size: 1451 Fragments arranged by size: 1451 1158 936 871 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2699 2729 2784 2814 1178 Size: 30 165 30 1296 30 55 30 4119 Fragments arranged by size: 4119 1296 165 55 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2127 2243 1186 Size: 941 116 4698 Fragments arranged by size: 4698 941 116 BseYI C'CCAG_C Cuts at: 4958 5454 4958 Size: 496 5259 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2564 1163 Size: 573 828 4354 Fragments arranged by size: 4354 828 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 3816 3901 4972 2313 Size: 1503 85 1071 3096 Fragments arranged by size: 3096 1503 1071 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2313 2600 2889 3074 3816 3901 4972 Size: 399 523 287 289 185 742 85 1071 Cuts at: 4972 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 523 399 289 287 185 85 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2586 2634 3110 844 Size: 1742 48 476 3489 Fragments arranged by size: 3489 1742 476 48 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4399 4586 1700 Size: 110 2589 187 2869 Fragments arranged by size: 2869 2589 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstYI r'GATC_y Cuts at: 1972 2856 3085 3452 3837 3854 5306 5392 5404 Size: 884 229 367 385 17 1452 86 12 Cuts at: 5404 5750 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 884 385 367 346 229 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2365 2550 513 Size: 552 939 361 185 3718 Fragments arranged by size: 3718 939 552 361 185 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2116 487 Size: 683 56 890 4126 Fragments arranged by size: 4126 890 683 56 BtsI GCAGTG_nn' Cuts at: 2970 3271 4065 4085 2970 Size: 301 794 20 4640 Fragments arranged by size: 4640 794 301 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2547 2737 3202 Size: 54 1425 50 275 18 717 190 465 Cuts at: 3202 4091 5720 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 717 465 275 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3685 1353 Size: 2332 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2167 2276 2363 2561 2650 2754 2854 Size: 393 535 109 87 198 89 104 100 Cuts at: 2854 3008 3818 4415 5202 5470 1239 Size: 154 810 597 787 268 1524 Fragments arranged by size: 1524 810 787 597 535 393 268 198 154 109 104 100 89 87 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2305 2305 Size: 5755 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 4902 1678 Size: 3224 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2083 2115 4396 4428 4470 Size: 32 135 32 511 32 2281 32 42 Cuts at: 4470 4502 1373 Size: 32 2626 Fragments arranged by size: 2626 2281 511 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2643 4869 5053 1305 Size: 1338 2226 184 2007 Fragments arranged by size: 2226 2007 1338 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2549 10 Size: 54 1425 343 717 3216 Fragments arranged by size: 3216 1425 717 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3713 4072 4231 518 Size: 648 2547 359 159 2042 Fragments arranged by size: 2547 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2254 3171 3848 4996 5241 1606 Size: 457 191 917 677 1148 245 2120 Fragments arranged by size: 2120 1148 917 677 457 245 191 NcoI C'CATG_G Cuts at: 513 1065 2004 2365 2550 513 Size: 552 939 361 185 3718 Fragments arranged by size: 3718 939 552 361 185 NdeI CA'TA_TG Cuts at: 387 2830 387 Size: 2443 3312 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2405 2943 3113 4658 1132 Size: 97 1176 538 170 1545 2229 Fragments arranged by size: 2229 1545 1176 538 170 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 2401 4654 2401 Size: 2253 3502 PflMI CCAn_nnn'nTGG Cuts at: 1108 2066 1108 Size: 958 4797 PfoI T'CCnGG_A Cuts at: 2717 2717 Size: 5755 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PmlI CAC'GTG Cuts at: 2526 2526 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2699 2731 3824 3856 5337 5369 2699 Size: 32 1093 32 1481 32 3085 Fragments arranged by size: 3085 1481 1093 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PstI C_TGCA'G Cuts at: 830 2376 3107 830 Size: 1546 731 3478 Fragments arranged by size: 3478 1546 731 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 PvuII CAG'CTG Cuts at: 2063 2254 2063 Size: 191 5564 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2372 3103 4238 4919 5110 826 Size: 219 1327 731 1135 681 191 1471 Fragments arranged by size: 1471 1327 1135 731 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2473 3859 4760 5022 820 Size: 197 926 530 1386 901 262 1553 Fragments arranged by size: 1553 1386 926 901 530 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 2943 3113 1132 Size: 1811 170 3774 Fragments arranged by size: 3774 1811 170 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 2037 1517 Size: 520 5235 StyI C'CwwG_G Cuts at: 513 1065 2004 2213 2365 2392 2550 3194 513 Size: 552 939 209 152 27 158 644 3074 Fragments arranged by size: 3074 939 644 552 209 158 152 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3782 3799 3952 4137 4582 325 Size: 1255 2202 17 153 185 445 1498 Fragments arranged by size: 2202 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2152 2184 2784 2816 5715 5747 2152 Size: 32 600 32 2899 32 2160 Fragments arranged by size: 2899 2160 600 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2558 2638 1316 Size: 1242 80 4433 Fragments arranged by size: 4433 1242 80 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2778 2878 2778 Size: 100 5655 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI