Restriction map for pFN21AE0945
 (Linear) (Six-base) MAPSORT of: KIEE0945.seq  Check: 8372  from: 1  to: 1050



KIEE0945.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     271     645    1050 
   Size:        162     109     374     405

  Fragments arranged by size: 

                 405     374     162     109
 
AflIII A'CryG_T

Cuts at:      0     396     518    1050 
   Size:        396     122     532

  Fragments arranged by size: 

                 532     396     122
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     329    1050 
   Size:        329     721
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     694     726    1050 
   Size:        694      32     324

  Fragments arranged by size: 

                 694     324      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     375     645    1050 
   Size:        375     270     405

  Fragments arranged by size: 

                 405     375     270
 
AseI AT'TA_AT

Cuts at:      0     566    1050 
   Size:        566     484
 
AvaI C'yCGr_G

Cuts at:      0      41    1050 
   Size:         41    1009
 
BanI G'GyrC_C

Cuts at:      0      48    1050 
   Size:         48    1002
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     634    1050 
   Size:        634     416
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     999    1050 
   Size:        999      51
 
BclI T'GATC_A

Cuts at:      0      74    1050 
   Size:         74     976
 
BglI GCCn_nnn'nGGC

Cuts at:      0     590    1050 
   Size:        590     460
 
Bme1580I G_kGCm'C

Cuts at:      0     595     884    1050 
   Size:        595     289     166

  Fragments arranged by size: 

                 595     289     166
 
BmgBI CAC'GTC

Cuts at:      0     795    1050 
   Size:        795     255
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     633    1050 
   Size:        633     417
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     965    1050 
   Size:        965      85
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     556     749     849    1003    1050 
   Size:        358     198     193     100     154      47

  Fragments arranged by size: 

                 358     198     193     154     100      47
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     489    1050 
   Size:        489     561
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     979    1050 
   Size:        979      71
 
BsaAI yAC'GTr

Cuts at:      0     521    1050 
   Size:        521     529
 
BsaHI Gr'CG_yC

Cuts at:      0     686    1050 
   Size:        686     364
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     694     724     779     809    1050 
   Size:        694      30      55      30     241

  Fragments arranged by size: 

                 694     241      55      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     122     238    1050 
   Size:        122     116     812

  Fragments arranged by size: 

                 812     122     116
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559    1050 
   Size:        559     491
 
BsiHKAI G_wGCw'C

Cuts at:      0     308    1050 
   Size:        308     742
 
Bsp1286I G_dGCh'C

Cuts at:      0     308     595     884    1050 
   Size:        308     287     289     166

  Fragments arranged by size: 

                 308     289     287     166
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     581     629    1050 
   Size:        581      48     421

  Fragments arranged by size: 

                 581     421      48
 
BstYI r'GATC_y

Cuts at:      0     851    1050 
   Size:        851     199
 
BtgI C'CryG_G

Cuts at:      0     360     545    1050 
   Size:        360     185     505

  Fragments arranged by size: 

                 505     360     185
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     111    1050 
   Size:        111     939
 
BtsI GCAGTG_nn'

Cuts at:      0     965    1050 
   Size:        965      85
 
EaeI y'GGCC_r

Cuts at:      0     542     732    1050 
   Size:        542     190     318

  Fragments arranged by size: 

                 542     318     190
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     271     358     556     645     749     849    1003 
   Size:        162     109      87     198      89     104     100     154

Cuts at:   1003    1050 
   Size:         47

  Fragments arranged by size: 

                 198     162     154     109     104     100      89      87
                  47
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     300    1050 
   Size:        300     750
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      78     110    1050 
   Size:         78      32     940

  Fragments arranged by size: 

                 940      78      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     638    1050 
   Size:        638     412
 
MscI TGG'CCA

Cuts at:      0     544    1050 
   Size:        544     506
 
MspA1I CmG'CkG

Cuts at:      0      58     249    1050 
   Size:         58     191     801

  Fragments arranged by size: 

                 801     191      58
 
NcoI C'CATG_G

Cuts at:      0     360     545    1050 
   Size:        360     185     505

  Fragments arranged by size: 

                 505     360     185
 
NdeI CA'TA_TG

Cuts at:      0     825    1050 
   Size:        825     225
 
NspI r_CATG'y

Cuts at:      0     400     938    1050 
   Size:        400     538     112

  Fragments arranged by size: 

                 538     400     112
 
PciI A'CATG_T

Cuts at:      0     396    1050 
   Size:        396     654
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      61    1050 
   Size:         61     989
 
PfoI T'CCnGG_A

Cuts at:      0     712    1050 
   Size:        712     338
 
PmlI CAC'GTG

Cuts at:      0     521    1050 
   Size:        521     529
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     694     726    1050 
   Size:        694      32     324

  Fragments arranged by size: 

                 694     324      32
 
PstI C_TGCA'G

Cuts at:      0     371    1050 
   Size:        371     679
 
PvuII CAG'CTG

Cuts at:      0      58     249    1050 
   Size:         58     191     801

  Fragments arranged by size: 

                 801     191      58
 
SfcI C'TryA_G

Cuts at:      0     367    1050 
   Size:        367     683
 
SmlI C'TyrA_G

Cuts at:      0     468    1050 
   Size:        468     582
 
SphI G_CATG'C

Cuts at:      0     938    1050 
   Size:        938     112
 
StuI AGG'CCT

Cuts at:      0      32    1050 
   Size:         32    1018
 
StyI C'CwwG_G

Cuts at:      0     208     360     387     545    1050 
   Size:        208     152      27     158     505

  Fragments arranged by size: 

                 505     208     158     152      27
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     147     179     779     811    1050 
   Size:        147      32     600      32     239

  Fragments arranged by size: 

                 600     239     147      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     553     633    1050 
   Size:        553      80     417

  Fragments arranged by size: 

                 553     417      80
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     773     873    1050 
   Size:        773     100     177

  Fragments arranged by size: 

                 773     177     100
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     AloI    AlwNI     AseI     AvaI     BanI
     BbsI    BciVI     BclI     BglI Bme1580I    BmgBI     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI     BsgI
  BsiHKAI Bsp1286I    BspMI    BstYI     BtgI    BtgZI     BtsI     EaeI
  Eco57MI     FalI    Hin4I     MmeI     MscI   MspA1I     NcoI     NdeI
     NspI     PciI    PflMI     PfoI     PmlI     PpiI     PstI    PvuII
     SfcI     SmlI     SphI     StuI     StyI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     ApaI    ApaLI     ApoI     AscI    AsiSI    AvrII
     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BfrBI    BglII
     BlpI     BmtI   Bpu10I    BsaBI    BsaWI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MslI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PmeI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII     TatI     XbaI
     XhoI     XmaI     XmnI     ZraI
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