(Circular) (Six-base) MAPSORT of: pFN21AE0944.seq Check: 7985 from: 1 to: 5734 pFN21AE0944 5734 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2441 2441 Size: 5734 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3000 3544 278 Size: 53 83 186 936 1464 544 2468 Fragments arranged by size: 2468 1464 936 544 186 83 53 AccI GT'mk_AC Cuts at: 1937 2021 3077 1937 Size: 84 1056 4594 Fragments arranged by size: 4594 1056 84 Acc65I G'GTAC_C Cuts at: 2757 3055 2757 Size: 298 5436 AclI AA'CG_TT Cuts at: 1563 3033 3861 4234 1563 Size: 1470 828 373 3063 Fragments arranged by size: 3063 1470 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2776 3797 5181 5449 1632 Size: 1144 1021 1384 268 1917 Fragments arranged by size: 1917 1384 1144 1021 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 2712 4633 5610 1203 Size: 1509 1921 977 1327 Fragments arranged by size: 1921 1509 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5734 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2120 2152 2120 Size: 32 5702 AlwNI CAG_nnn'CTG Cuts at: 3477 5049 3477 Size: 1572 4162 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 2543 2907 2950 3043 3269 3421 5530 1493 Size: 1050 364 43 93 226 152 2109 1697 Fragments arranged by size: 2109 1697 1050 364 226 152 93 43 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2140 2150 2571 2695 3059 1870 Size: 73 197 10 421 124 364 4545 Fragments arranged by size: 4545 421 364 197 124 73 10 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2308 2757 2989 3055 4510 Size: 325 217 627 521 449 232 66 1455 Cuts at: 4510 618 Size: 1842 Fragments arranged by size: 1842 1455 627 521 449 325 232 217 66 BanII G_rGCy'C Cuts at: 1391 2109 2147 2883 3053 1391 Size: 718 38 736 170 4072 Fragments arranged by size: 4072 736 718 170 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2601 928 Size: 971 702 4061 Fragments arranged by size: 4061 971 702 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3925 3959 1903 Size: 34 1988 34 3678 Fragments arranged by size: 3678 1988 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BfrBI ATG'CAT Cuts at: 2416 2416 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4345 136 Size: 107 122 71 631 605 2673 1525 Fragments arranged by size: 2673 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2670 5729 2670 Size: 3059 2675 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 3795 4951 1790 Size: 2005 1156 2573 Fragments arranged by size: 2573 2005 1156 BmgBI CAC'GTC Cuts at: 1318 2713 1318 Size: 1395 4339 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3465 4423 442 Size: 416 53 83 399 24 2048 958 1753 Fragments arranged by size: 2048 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 4394 1239 Size: 995 2160 2579 Fragments arranged by size: 2579 2160 995 Bpu10I CC'TnA_GC Cuts at: 2967 2967 Size: 5734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2516 3859 4724 5022 2516 Size: 1343 865 298 3228 Fragments arranged by size: 3228 1343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2214 2366 4397 882 Size: 617 715 152 2031 2219 Fragments arranged by size: 2219 2031 715 617 152 BsaAI yAC'GTr Cuts at: 493 1204 2659 3525 493 Size: 711 1455 866 2702 Fragments arranged by size: 2702 1455 866 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2598 2997 3541 3923 Size: 53 83 186 936 1065 399 544 382 Cuts at: 3923 4558 275 Size: 635 1451 Fragments arranged by size: 1451 1065 936 635 544 399 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4167 4565 4839 4986 5397 4167 Size: 398 274 147 411 4504 Fragments arranged by size: 4504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 2211 2241 2252 Size: 30 165 30 717 30 61 30 11 Cuts at: 2252 2282 1178 Size: 30 4630 Fragments arranged by size: 4630 717 165 61 30 30 30 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2273 2465 2468 1186 Size: 1087 192 3 4452 Fragments arranged by size: 4452 1087 192 3 BseYI C'CCAG_C Cuts at: 2108 2780 4937 5433 2108 Size: 672 2157 496 2409 Fragments arranged by size: 2409 2157 672 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2231 1163 Size: 573 495 4666 Fragments arranged by size: 4666 573 495 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3184 3945 4973 1540 Size: 275 186 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3795 3880 4951 3795 Size: 85 1071 4578 Fragments arranged by size: 4578 1071 85 BsmI GAATG_Cn' Cuts at: 2416 2865 3245 3338 2416 Size: 449 380 93 4812 Fragments arranged by size: 4812 449 380 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2950 2950 Size: 5734 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2147 2883 3053 3795 3880 4951 Size: 399 319 38 736 170 742 85 1071 Cuts at: 4951 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 736 399 319 170 85 38 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2441 3089 844 Size: 1597 648 3489 Fragments arranged by size: 3489 1597 648 BsrBI CCG'CTC Cuts at: 1394 1823 2890 3621 1394 Size: 429 1067 731 3507 Fragments arranged by size: 3507 1067 731 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2170 4229 4403 65 Size: 1183 922 2059 174 1396 Fragments arranged by size: 2059 1396 1183 922 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4378 4565 1700 Size: 110 2568 187 2869 Fragments arranged by size: 2869 2568 187 110 BsrGI T'GTAC_A Cuts at: 96 2736 96 Size: 2640 3094 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 2963 794 Size: 2169 3565 BstBI TT'CG_AA Cuts at: 2076 4538 2076 Size: 2462 3272 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstXI CCAn_nnnn'nTGG Cuts at: 2749 2749 Size: 5734 BstYI r'GATC_y Cuts at: 1972 2670 2799 3064 3431 3816 3833 5285 5371 Size: 698 129 265 367 385 17 1452 86 Cuts at: 5371 5383 5729 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 698 385 367 346 265 129 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2100 513 Size: 552 1035 4147 Fragments arranged by size: 4147 1035 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4995 Fragments arranged by size: 4995 683 56 BtsI GCAGTG_nn' Cuts at: 2496 2497 3250 4044 4064 2496 Size: 1 753 794 20 4166 Fragments arranged by size: 4166 794 753 20 1 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5699 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2809 3181 4070 Size: 54 1425 50 275 18 979 372 889 Cuts at: 4070 5699 8 Size: 1629 43 Fragments arranged by size: 1629 1425 979 889 372 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2073 2556 2795 3003 3664 1353 Size: 720 483 239 208 661 3423 Fragments arranged by size: 3423 720 661 483 239 208 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4321 4695 4841 387 Size: 1064 138 2732 374 146 1280 Fragments arranged by size: 2732 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2776 3797 4394 5181 5449 1239 Size: 393 602 542 1021 597 787 268 1524 Fragments arranged by size: 1524 1021 787 602 597 542 393 268 EcoO109I rG'GnC_Cy Cuts at: 2527 2527 Size: 5734 EcoRI G'AATT_C Cuts at: 2950 3043 3421 2950 Size: 93 378 5263 Fragments arranged by size: 5263 378 93 EcoRV GAT'ATC Cuts at: 2284 2284 Size: 5734 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 4881 1678 Size: 3203 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 2615 2647 2819 Size: 32 135 32 500 32 511 32 172 Cuts at: 2819 2851 4375 4407 4449 4481 1373 Size: 32 1524 32 42 32 2626 Fragments arranged by size: 2626 1524 511 500 172 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3001 3078 3324 669 Size: 988 281 1063 77 246 3079 Fragments arranged by size: 3079 1063 988 281 246 77 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 2761 3059 2761 Size: 298 5436 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2184 2658 4848 5032 1305 Size: 879 474 2190 184 2007 Fragments arranged by size: 2190 2007 879 474 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2811 10 Size: 54 1425 343 979 2933 Fragments arranged by size: 2933 1425 979 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2502 2747 3692 4051 4210 518 Size: 648 1336 245 945 359 159 2042 Fragments arranged by size: 2042 1336 945 648 359 245 159 MspA1I CmG'CkG Cuts at: 1606 2102 2253 2289 2986 3150 3827 4975 5220 Size: 496 151 36 697 164 677 1148 245 Cuts at: 5220 1606 Size: 2120 Fragments arranged by size: 2120 1148 697 677 496 245 164 151 36 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5182 NdeI CA'TA_TG Cuts at: 387 2959 387 Size: 2572 3162 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NsiI A_TGCA'T Cuts at: 2418 2418 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 2416 3092 4637 1132 Size: 97 1187 676 1545 2229 Fragments arranged by size: 2229 1545 1187 676 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5734 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5734 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2120 2152 3803 3835 5316 5348 2120 Size: 32 1651 32 1481 32 2506 Fragments arranged by size: 2506 1651 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 2527 Size: 5734 PshAI GACnn'nnGTC Cuts at: 1613 2573 1613 Size: 960 4774 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PstI C_TGCA'G Cuts at: 830 3086 830 Size: 2256 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 PvuII CAG'CTG Cuts at: 2289 2986 2289 Size: 697 5037 SacII CC_GC'GG Cuts at: 2103 2103 Size: 5734 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 2931 3082 4217 4898 5089 826 Size: 219 1886 151 1135 681 191 1471 Fragments arranged by size: 1886 1471 1135 681 219 191 151 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 3061 1872 Size: 1189 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2531 3838 4739 5001 820 Size: 197 926 588 1307 901 262 1553 Fragments arranged by size: 1553 1307 926 901 588 262 197 SnaBI TAC'GTA Cuts at: 493 2659 3525 493 Size: 2166 866 2702 Fragments arranged by size: 2702 2166 866 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5734 StyI C'CwwG_G Cuts at: 513 1065 2841 2921 3173 513 Size: 552 1776 80 252 3074 Fragments arranged by size: 3074 1776 552 252 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2357 3761 3778 3931 4116 4561 325 Size: 1255 777 1404 17 153 185 445 1498 Fragments arranged by size: 1498 1404 1255 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2736 3980 5692 Size: 274 80 33 51 1443 759 1244 1712 Cuts at: 5692 96 Size: 138 Fragments arranged by size: 1712 1443 1244 759 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2252 2284 5694 5726 2252 Size: 32 3410 32 2260 Fragments arranged by size: 3410 2260 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2833 1316 Size: 1517 4217 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 3059 1870 Size: 1189 4545 XmnI GAAnn'nnTTC Cuts at: 3863 3863 Size: 5734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2998 3542 276 Size: 53 83 186 936 1464 544 2468 Fragments arranged by size: 2468 1464 936 544 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI