Restriction map for
pFN21AE0930
(Circular) (Six-base) MAPSORT of: pFN21AE0930.seq Check: 6100 from: 1 to: 5860
pFN21AE0930 5860 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3923 5307 5575 1632
Size: 2291 1384 268 1917
Fragments arranged by size:
2291 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 2479 3034 3603 5175 2479
Size: 555 569 1572 3164
Fragments arranged by size:
3164 1572 569 555
ApaI G_GGCC'C
Cuts at: 2530 2615 2530
Size: 85 5775
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 2150 2938 3169 3395 3547 5656 1493
Size: 657 788 231 226 152 2109 1697
Fragments arranged by size:
2109 1697 788 657 231 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 3087 3185 1870
Size: 73 1144 98 4545
Fragments arranged by size:
4545 1144 98 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2842 2957 3181 4636 618
Size: 325 217 627 1055 115 224 1455 1842
Fragments arranged by size:
1842 1455 1055 627 325 224 217 115
BanII G_rGCy'C
Cuts at: 1391 2530 2615 3179 1391
Size: 1139 85 564 4072
Fragments arranged by size:
4072 1139 564 85
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4889
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 2347 1548
Size: 799 5061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 2530 2615 3921 5077 1790
Size: 740 85 1306 1156 2573
Fragments arranged by size:
2573 1306 1156 740 85
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2965 3033 3591
Size: 416 53 83 399 24 1548 68 558
Cuts at: 3591 4549 442
Size: 958 1753
Fragments arranged by size:
1753 1548 958 558 416 399 83 68
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2680 2813 2834 4520 1239
Size: 1262 179 133 21 1686 2579
Fragments arranged by size:
2579 1686 1262 179 133 21
Bpu10I CC'TnA_GC
Cuts at: 2463 3016 2463
Size: 553 5307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2236 3985 4850 5148 2236
Size: 1749 865 298 2948
Fragments arranged by size:
2948 1749 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3089 4523 882
Size: 617 1590 1434 2219
Fragments arranged by size:
2219 1590 1434 617
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3667 4049 4684 275
Size: 53 83 186 936 2134 382 635 1451
Fragments arranged by size:
2134 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4293 4691 4965 5112 5523 4293
Size: 398 274 147 411 4630
Fragments arranged by size:
4630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5635
Fragments arranged by size:
5635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3139 3152 1186
Size: 1953 13 3894
Fragments arranged by size:
3894 1953 13
BseYI C'CCAG_C
Cuts at: 2118 2529 5063 5559 2118
Size: 411 2534 496 2419
Fragments arranged by size:
2534 2419 496 411
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2058 1163
Size: 573 322 4965
Fragments arranged by size:
4965 573 322
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3921 4006 5077 3921
Size: 85 1071 4704
Fragments arranged by size:
4704 1071 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2530 2615 3179 3921 4006 5077 1391
Size: 399 740 85 564 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 740 564 399 85 85
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3215 844
Size: 2371 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2088 2406 4355 4529 65
Size: 1183 840 318 1949 174 1396
Fragments arranged by size:
1949 1396 1183 840 318 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2617 4504 4691 1700
Size: 110 807 1887 187 2869
Fragments arranged by size:
2869 1887 807 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 2742 2818 3190 3557 3942 3959 5411 5497
Size: 770 76 372 367 385 17 1452 86
Cuts at: 5497 5509 5855 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 770 385 372 367 346 86
76 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5308
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 3067 3073 3376 4170 4190 3067
Size: 6 303 794 20 4737
Fragments arranged by size:
4737 794 303 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 2385 3164 2385
Size: 779 5081
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3307 4196 5825
Size: 54 1425 50 275 18 1477 889 1629
Cuts at: 5825 8
Size: 43
Fragments arranged by size:
1629 1477 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2362 3790 1353
Size: 1009 1428 3423
Fragments arranged by size:
3423 1428 1009
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2515 4447 4821 4967 387
Size: 1064 138 926 1932 374 146 1280
Fragments arranged by size:
1932 1280 1064 926 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 2680 2813 2834 3923 4520 5307
Size: 393 869 179 133 21 1089 597 787
Cuts at: 5307 5575 1239
Size: 268 1524
Fragments arranged by size:
1524 1089 869 787 597 393 268 179
133 21
EcoO109I rG'GnC_Cy
Cuts at: 2122 2611 2885 2122
Size: 489 274 5097
Fragments arranged by size:
5097 489 274
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
EcoRV GAT'ATC
Cuts at: 2875 2875
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 5007 1678
Size: 3329 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2044 2076 2282 2314 3134
Size: 32 135 32 472 32 206 32 820
Cuts at: 3134 3166 4501 4533 4575 4607 1373
Size: 32 1335 32 42 32 2626
Fragments arranged by size:
2626 1335 820 472 206 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4038
Fragments arranged by size:
4038 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3818 4177 4336 518
Size: 648 2652 359 159 2042
Fragments arranged by size:
2652 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2533 2641 3276 3953 5101 5346 1606
Size: 927 108 635 677 1148 245 2120
Fragments arranged by size:
2120 1148 927 677 635 245 108
NaeI GCC'GGC
Cuts at: 2619 2619
Size: 5860
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5308
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NgoMIV G'CCGG_C
Cuts at: 2617 2617
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 3218 4763 1132
Size: 97 1989 1545 2229
Fragments arranged by size:
2229 1989 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 2885 2122
Size: 763 5097
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 2012 3430 2012
Size: 1418 4442
PspOMI G'GGCC_C
Cuts at: 2526 2611 2526
Size: 85 5775
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2532 2564 2532
Size: 32 5828
PstI C_TGCA'G
Cuts at: 830 3212 830
Size: 2382 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2533 2641 2533
Size: 108 5752
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SanDI GG'GwC_CC
Cuts at: 2122 2122
Size: 5860
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 2763 4108 5820 2763
Size: 1345 1712 2803
Fragments arranged by size:
2803 1712 1345
SexAI A'CCwGG_T
Cuts at: 2571 2571
Size: 5860
SfcI C'TryA_G
Cuts at: 826 1045 2549 3208 4343 5024 5215 826
Size: 219 1504 659 1135 681 191 1471
Fragments arranged by size:
1504 1471 1135 681 659 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2215 3964 4865 5127 820
Size: 197 926 272 1749 901 262 1553
Fragments arranged by size:
1749 1553 926 901 272 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 2986 3299 513
Size: 552 1921 313 3074
Fragments arranged by size:
3074 1921 552 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3887 3904 4057 4242 4687 325
Size: 1255 2307 17 153 185 445 1498
Fragments arranged by size:
2307 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2761 4106 5818
Size: 274 80 33 51 1443 784 1345 1712
Cuts at: 5818 96
Size: 138
Fragments arranged by size:
1712 1443 1345 784 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5820 5852 5820
Size: 32 5828
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspXI RsrII SacI SacII SapI
SfiI SfoI SrfI SwaI XcmI